Rh6BG431300

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000265
Physical Location & Seq
Forward (+)
65072153 .. 65074646
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG431300.1

Sequence Viewer

Length: 1326 bp
ATGGAGATGCCTATCCACGGATCCGCTTCTCTTCCAAATAATTTTATCAAGAGAGAAAGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATCAGAGGAATTATCCCCGGTACTGTTCTCAGTTTCCTTGAGTCTGAGCTTCAGAAGCTGGATGGTGAGGATGCAAGACTGGCAGATTATTTTGATGTCATTGCAGGAACTAGTACTGGTGGCCTTGTGACTGCCATGCTTACCGCCCCGGATGAAAACGGCCGTCCATTATTTGCTGCTAAAGATATCAACAAATTTTACCTTGAACACTGCCCTAAGATATTTCCCCAAGATAGTTGCAAAATTGATTGTCTTCCATTAACTCCAATAACTGATGCGGTGGCAGCGGTAAAAGCTTTCAGAGGGCCAAAGTATGATGGGGAGTATTTACATAATATAGTGAAGGAAAAGTTAGGAGACACAAGATTGCATCAGACATTGACCAACGTCGTAATTCCCACGTTTGATATAAAGAGGCTCCAACCAACCATCTTCTCCAGCTTTGAGGTGAGAAAGAACAGTAGCCTAGATGCTTTACTCTCGGATATATGCATTGCAACTTCAGCAGCCCCAACCTTTTTTCCAGCCCATCATTTTGAAACCGAAAATTCCACGACTGGTCAAATAAGAGAATTTGATCTCATTGATGGTGGTGTTGCTGCTAATAACCCAGCTTTGGTGGCTATAAGTGAAGTGTCAAAAGAAATTTACAAGGGAAATCCCGACTTTTTTACATTGAAGGCAACTGAATACGCCCGGTTTCTAGTTGTATCATTGGGTACTGGTTCAGCAAAATGTGATGAGAAGTACGATGCCCAAAATGCAGCTAAGTGGGGTATTTTGGGATGGTTGATCGGAGGCGGTTCCTGTCCGTTGGTTGATATATTTACTCAAGCAAGTAGTGATATGGTTGACTTCCACCTTTCTACAGTGTACCAAGCCCTTCACTCTGAGAAAAATTACCTAAGAATCCAGGATGATGATTTGGGTAAGACATTGTGTTCTGTGGATATTGCTACAGAAGAAAACTTGCAAAATCTTGTAGAAGTTGGAGAAAATTTGTTAAAGAAACCAGTTTCCCGAGTAAACTTGGAAACCGGTATCCATGAGCCTGCCCATCATTCTACCAACGAAGAGGCTCTAGTAAGGGTAGCTGGAATTTTGTCCAAGGAAAGGAGAGTCCGTGAAATGAGATCGCCACAGGGAAAGAAGGCCACAACAACAACCAGCTCCAACTTGTCATTCTCCTTCATCAATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

441

Amino Acids

48.28

Weight (kDa)

5.45

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 35 - 250 2.4e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 24, 267, 401, 410, 924
AclWI GGATC 2 cut(s) 15, 28
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 6 cut(s) 317, 670, 695, 768, 1120, 1221
AcuI CTGAAG 3 cut(s) 53, 158, 609
AfaI GTAC 5 cut(s) 145, 238, 844, 872, 998
AfiI CCNNNNNNNGG 4 cut(s) 17, 83, 739, 1236
AgeI ACCGGT 1 cut(s) 1160
AgsI TTSAA 3 cut(s) 329, 662, 802
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 1 cut(s) 1035
AjuI GAANNNNNNNTTGG 2 cut(s) 1289, 1321
AluBI AGCT 8 cut(s) 172, 181, 419, 564, 737, 890, 1217, 1293
AluI AGCT 8 cut(s) 172, 181, 419, 564, 737, 890, 1217, 1293
Alw26I GTCTC 2 cut(s) 78, 474
AlwI GGATC 2 cut(s) 15, 28
AlwNI CAGNNNCTG 1 cut(s) 181
Ama87I CYCGRG 1 cut(s) 1143
AoxI GGCC 4 cut(s) 244, 283, 428, 1275
ApeKI GCWGC 5 cut(s) 299, 407, 629, 722, 887
ApoI RAATTY 6 cut(s) 317, 670, 695, 768, 1120, 1221
AsiGI ACCGGT 1 cut(s) 1160
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 3 cut(s) 141, 272, 820
AsuHPI GGTGA 2 cut(s) 200, 583
AvaI CYCGRG 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 5 cut(s) 286, 419, 641, 709, 899
BccI CCATC 8 cut(s) 107, 179, 434, 560, 660, 704, 903, 1188
BceAI ACGGC 2 cut(s) 270, 298
BciT130I CCWGG 1 cut(s) 1037
BciVI GTATCC 1 cut(s) 1175
BcnI CCSGG 3 cut(s) 141, 272, 820
BcoDI GTCTC 2 cut(s) 78, 474
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 4 cut(s) 234, 590, 827, 1205
BfmI CTRYAG 2 cut(s) 990, 1080
BfuI GTATCC 1 cut(s) 1175
BisI GCNGC 5 cut(s) 300, 408, 630, 723, 888
BlsI GCNGC 5 cut(s) 301, 409, 631, 724, 889
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 4 cut(s) 141, 272, 820, 1037
BmeT110I CYCGRG 1 cut(s) 1143
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 22, 542, 928
BmrFI CCNGG 4 cut(s) 141, 272, 820, 1037
BmsI GCATC 5 cut(s) 184, 388, 502, 583, 865
BoxI GACNNNNGTC 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 1 cut(s) 544
BpuEI CTTGAG 2 cut(s) 182, 939
BpuMI CCSGG 3 cut(s) 141, 272, 820
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 16, 139, 270, 1230
BsaWI WCCGGW 1 cut(s) 1160
BsaXI ACNNNNNCTCC 2 cut(s) 1231, 1261
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 83, 739, 1236
Bse118I RCCGGY 1 cut(s) 1160
Bse1I ACTGG 5 cut(s) 207, 244, 685, 850, 1136
Bse3DI GCAATG 2 cut(s) 222, 615
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 1 cut(s) 1037
BseDI CCNNGG 4 cut(s) 16, 139, 270, 1230
BseGI GGATG 5 cut(s) 190, 199, 280, 914, 1045
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 17, 83, 739, 1236
BseMI GCAATG 2 cut(s) 222, 615
BseMII CTCAG 3 cut(s) 159, 166, 1005
BseNI ACTGG 5 cut(s) 207, 244, 685, 850, 1136
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 5 cut(s) 286, 419, 641, 709, 899
BseYI CCCAGC 1 cut(s) 733
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 4 cut(s) 246, 285, 430, 1277
BshTI ACCGGT 1 cut(s) 1160
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 1143
BsiSI CCGG 4 cut(s) 141, 272, 820, 1161
BslI CCNNNNNNNGG 4 cut(s) 17, 83, 739, 1236
BsmAI GTCTC 2 cut(s) 78, 474
BsnI GGCC 4 cut(s) 246, 285, 430, 1277
BsoBI CYCGRG 1 cut(s) 1143
Bsp143I GATC 5 cut(s) 20, 59, 700, 915, 1256
BspACI CCGC 5 cut(s) 24, 267, 401, 410, 924
BspANI GGCC 4 cut(s) 246, 285, 430, 1277
BspCNI CTCAG 3 cut(s) 160, 165, 1006
BspLI GGNNCC 3 cut(s) 22, 542, 928
BspPI GGATC 2 cut(s) 15, 28
BsrDI GCAATG 2 cut(s) 222, 615
BsrFI RCCGGY 1 cut(s) 1160
BsrI ACTGG 5 cut(s) 207, 244, 685, 850, 1136
BssAI RCCGGY 1 cut(s) 1160
BssECI CCNNGG 4 cut(s) 16, 139, 270, 1230
BssMI GATC 5 cut(s) 20, 59, 700, 915, 1256
BssT1I CCWWGG 1 cut(s) 1230
Bst2UI CCWGG 1 cut(s) 1037
Bst4CI ACNGT 4 cut(s) 100, 148, 584, 994
Bst6I CTCTTC 2 cut(s) 36, 1191
BstC8I GCNNGC 1 cut(s) 1176
BstDEI CTNAG 7 cut(s) 104, 152, 168, 339, 891, 1014, 1028
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 5 cut(s) 190, 199, 280, 914, 1045
BstKTI GATC 5 cut(s) 23, 62, 703, 918, 1259
BstMAI GTCTC 2 cut(s) 78, 474
BstMBI GATC 5 cut(s) 20, 59, 700, 915, 1256
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 7 cut(s) 178, 203, 407, 416, 626, 743, 884
BstNI CCWGG 1 cut(s) 1037
BstPAI GACNNNNGTC 1 cut(s) 509
BstSCI CCNGG 4 cut(s) 139, 270, 818, 1035
BstSFI CTRYAG 2 cut(s) 990, 1080
BstV1I GCAGC 5 cut(s) 286, 419, 641, 709, 899
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BstZI CGGCCG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 1175
BsuRI GGCC 4 cut(s) 246, 285, 430, 1277
BtgI CCRYGG 1 cut(s) 16
BtsCI GGATG 5 cut(s) 190, 199, 280, 914, 1045
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 96, 331, 999
Cac8I GCNNGC 1 cut(s) 1176
CaiI CAGNNNCTG 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 1160
Cfr13I GGNCC 1 cut(s) 428
Csp6I GTAC 5 cut(s) 144, 237, 843, 871, 997
CspAI ACCGGT 1 cut(s) 1160
CviAII CATG 2 cut(s) 259, 1169
CviQI GTAC 5 cut(s) 144, 237, 843, 871, 997
DdeI CTNAG 7 cut(s) 104, 152, 168, 339, 891, 1014, 1028
DpnI GATC 5 cut(s) 22, 61, 702, 917, 1258
DpnII GATC 5 cut(s) 20, 59, 700, 915, 1256
EaeI YGGCCR 1 cut(s) 283
EagI CGGCCG 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 36, 1191
EarI CTCTTC 2 cut(s) 36, 1191
EclXI CGGCCG 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 1230
Eco32I GATATC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 283
Eco57I CTGAAG 3 cut(s) 53, 158, 609
Eco88I CYCGRG 1 cut(s) 1143
EcoRII CCWGG 1 cut(s) 1035
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 1230
EcoT22I ATGCAT 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 1230
FaeI CATG 2 cut(s) 262, 1172
FatI CATG 2 cut(s) 258, 1168
Fnu4HI GCNGC 5 cut(s) 300, 408, 630, 723, 888
FokI GGATG 5 cut(s) 197, 206, 287, 921, 1052
Fsp4HI GCNGC 5 cut(s) 300, 408, 630, 723, 888
FspBI CTAG 4 cut(s) 234, 590, 827, 1205
GluI GCNGC 5 cut(s) 300, 408, 630, 723, 888
GsaI CCCAGC 1 cut(s) 737
GsuI CTGGAG 1 cut(s) 544
HaeIII GGCC 4 cut(s) 246, 285, 430, 1277
HapII CCGG 4 cut(s) 141, 272, 820, 1161
Hin1II CATG 2 cut(s) 262, 1172
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 4 cut(s) 123, 164, 1032, 1242
HpaII CCGG 4 cut(s) 141, 272, 820, 1161
HphI GGTGA 2 cut(s) 200, 583
Hpy166II GTNNAC 3 cut(s) 976, 997, 1150
Hpy188I TCNGA 8 cut(s) 128, 169, 177, 425, 498, 607, 920, 1015
Hpy188III TCNNGA 3 cut(s) 49, 785, 1143
Hpy8I GTNNAC 3 cut(s) 976, 997, 1150
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 6 cut(s) 77, 460, 796, 1016, 1267, 1321
HpyCH4III ACNGT 4 cut(s) 100, 148, 584, 994
HpyCH4IV ACGT 2 cut(s) 510, 524
HpyCH4V TGCA 8 cut(s) 197, 227, 363, 493, 615, 620, 887, 1096
HpyF10VI GCNNNNNNNGC 7 cut(s) 178, 203, 407, 416, 626, 743, 884
HpyF3I CTNAG 7 cut(s) 104, 152, 168, 339, 891, 1014, 1028
HpySE526I ACGT 2 cut(s) 510, 524
Hsp92II CATG 2 cut(s) 262, 1172
Kzo9I GATC 5 cut(s) 20, 59, 700, 915, 1256
LmnI GCTCC 2 cut(s) 546, 1298
Lsp1109I GCAGC 5 cut(s) 286, 419, 641, 709, 899
LweI GCATC 5 cut(s) 184, 388, 502, 583, 865
MaeI CTAG 4 cut(s) 234, 590, 827, 1205
MaeII ACGT 2 cut(s) 510, 524
MaeIII GTNAC 2 cut(s) 94, 250
MalI GATC 5 cut(s) 22, 61, 702, 917, 1258
MboI GATC 5 cut(s) 20, 59, 700, 915, 1256
MboII GAAGA 5 cut(s) 23, 368, 547, 1097, 1208
MflI RGATCY 1 cut(s) 20
MlyI GAGTC 2 cut(s) 173, 1251
MmeI TCCRAC 3 cut(s) 568, 1093, 1320
MnlI CCTC 9 cut(s) 101, 110, 122, 184, 419, 531, 562, 914, 1192
Mph1103I ATGCAT 1 cut(s) 617
MseI TTAA 3 cut(s) 383, 1127, 1324
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 4 cut(s) 141, 272, 820, 1161
MspR9I CCNGG 4 cut(s) 141, 272, 820, 1037
MvaI CCWGG 1 cut(s) 1037
MwoI GCNNNNNNNGC 7 cut(s) 178, 203, 407, 416, 626, 743, 884
NciI CCSGG 3 cut(s) 141, 272, 820
NdeII GATC 5 cut(s) 20, 59, 700, 915, 1256
NlaIII CATG 2 cut(s) 262, 1172
NlaIV GGNNCC 3 cut(s) 22, 542, 928
NmuCI GTSAC 2 cut(s) 94, 250
NsiI ATGCAT 1 cut(s) 617
PfeI GAWTC 2 cut(s) 123, 1032
PfoI TCCNGGA 1 cut(s) 1035
PinAI ACCGGT 1 cut(s) 1160
PkrI GCNGC 5 cut(s) 301, 409, 631, 724, 889
PleI GAGTC 2 cut(s) 172, 1250
PpsI GAGTC 2 cut(s) 172, 1250
PshAI GACNNNNGTC 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 1035
PspFI CCCAGC 1 cut(s) 733
PspGI CCWGG 1 cut(s) 1035
PspN4I GGNNCC 3 cut(s) 22, 542, 928
PspPI GGNCC 1 cut(s) 428
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 20
RsaI GTAC 5 cut(s) 145, 238, 844, 872, 998
RsaNI GTAC 5 cut(s) 144, 237, 843, 871, 997
SaqAI TTAA 3 cut(s) 383, 1127, 1324
SatI GCNGC 5 cut(s) 300, 408, 630, 723, 888
Sau3AI GATC 5 cut(s) 20, 59, 700, 915, 1256
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 2 cut(s) 173, 1251
ScrFI CCNGG 4 cut(s) 141, 272, 820, 1037
SfaNI GCATC 5 cut(s) 184, 388, 502, 583, 865
SfcI CTRYAG 2 cut(s) 990, 1080
SmlI CTYRAG 2 cut(s) 161, 954
SmoI CTYRAG 2 cut(s) 161, 954
SpeI ACTAGT 1 cut(s) 233
SsiI CCGC 5 cut(s) 24, 267, 401, 410, 924
SspMI CTAG 4 cut(s) 234, 590, 827, 1205
StyD4I CCNGG 4 cut(s) 139, 270, 818, 1035
StyI CCWWGG 1 cut(s) 1230
TaaI ACNGT 4 cut(s) 100, 148, 584, 994
TaiI ACGT 2 cut(s) 513, 527
TatI WGTACW 1 cut(s) 236
TfiI GAWTC 2 cut(s) 123, 1032
Tru1I TTAA 3 cut(s) 383, 1127, 1324
Tru9I TTAA 3 cut(s) 383, 1127, 1324
TscAI CASTG 3 cut(s) 103, 338, 999
TseFI GTSAC 2 cut(s) 94, 250
TseI GCWGC 5 cut(s) 299, 407, 629, 722, 887
Tsp45I GTSAC 2 cut(s) 94, 250
TspDTI ATGAA 2 cut(s) 291, 1303
TspGWI ACGGA 3 cut(s) 33, 924, 1235
TspRI CASTG 3 cut(s) 103, 338, 999
XapI RAATTY 6 cut(s) 317, 670, 695, 768, 1120, 1221
XspI CTAG 4 cut(s) 234, 590, 827, 1205
ZrmI AGTACT 1 cut(s) 238
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.