MD15G1085800.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000265
Physical Location & Seq
Reverse (-)
5918936 .. 5921920
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1085800.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGACAGAACCAAGAGTATACCATTACAGGCCCCAACTTACGGAAACTTAATAACGATTCTCAGCATTGATGGGGGTGGAATAAGAGGACTTATCCCAGGAACCATTCTTGGTTACCTTGAATCTGAGCTTCAGAAGTTGGATGGTGAGGATGCAAGAATCGCCGACTATTTTGATGTGATTGCAGGAACAAGCACCGGTGGTCTTGTGACTGCAATGCTTGCAACCCCAGATGACAACAACCGCCCTGTATTTGCTGCCAAGGATATTAAGGATTTCTACCTCACTCACTGCCCCAAAATTTTCCCCCAGAAACATTGTCCATTGTTTCCGAAAATGACAAAGATTATCAAAGCCCTATCAGGGCCAAAATATGATGGGAAGTATCTGCATGACTTGGTTGGGCAGAAACTTGGTTCTAAGAAACTGCATCAGACATTGACTAATGTTGTCATTCCCACATTTGACATAAAACAGCTCCAGCCAACTATATTTTCCAGCTATGAGGTGAAAAGTAAACCATTCTTTGATGCTTTACTCTCAGACATATGCATTGCAACCTCAGCTGCACCTACTTATCTACCAGCTCACTATTTTGAAACAATTGACCCAGAAGGGAATGTAAAAGACTTTAACCTTATAGACGGCGGTGTGGCTGCGAATAATCCGACTTTACTTGCAATTGGTGAAGTGACAAAGACAATAATAAGTGGGAGTGAAGATTTCTTCCCTATAAAGCCAATGGACTATGGGCGATTTCTAGTGATATCTCTAGGAACTGGCTCGGCAAAAGCTGAGTTCAAATACCGTGCGCATTCTGCAGCCAAGTGGGGTTTATTAGATTGGTTAACCAGCGGTGGTGGAACCCCAATAATTGATGTTTTCAGTCAATCAAGTTCTGATATGGTCGATTATCATCTTTCTGTGGTTTTCCAAGCCCTTCATTCGGAGAATAATTACCTTCGAATTCAGGATGACACATTAACTGGACAAGTATCTTCTGTGGACATAGCCGATAAGAAGAATTTGGATGATCTTCTCAAAGTGGGTGAAGGCCTGTTGAAAAAACCAGTTTCTAGGGTTAATTTGGAGACTGGCAGGGTGGAGCCTTCAAAACACGAGTCCAATGAAGAGGCTCTCACTAGGTTTGCAAAACTATTATCTGAAGAGAAGTGGCTTCGCCTTGCAAAGTCGCCTGTTGGACACGCCGCAAAGCCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

408

Amino Acids

44.53

Weight (kDa)

6.22

Isoelectric Point (pI)

27.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 228 3.7e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 813
AccI GTMKAC 1 cut(s) 19
AciI CCGC 4 cut(s) 244, 648, 855, 1209
AcsI RAATTY 3 cut(s) 300, 966, 1024
AcuI CTGAAG 2 cut(s) 116, 1185
AfiI CCNNNNNNNGG 3 cut(s) 41, 363, 946
AgeI ACCGGT 1 cut(s) 197
AgsI TTSAA 5 cut(s) 122, 599, 802, 1063, 1113
AjnI CCWGG 1 cut(s) 97
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 6 cut(s) 130, 478, 501, 566, 587, 794
AluI AGCT 6 cut(s) 130, 478, 501, 566, 587, 794
Alw26I GTCTC 1 cut(s) 1085
AoxI GGCC 3 cut(s) 30, 365, 1054
ApeKI GCWGC 4 cut(s) 257, 566, 656, 821
ApoI RAATTY 3 cut(s) 300, 966, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
AsiGI ACCGGT 1 cut(s) 197
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 2 cut(s) 31, 365
AsuHPI GGTGA 4 cut(s) 158, 520, 698, 1061
AsuII TTCGAA 1 cut(s) 964
BauI CACGAG 1 cut(s) 1118
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 4 cut(s) 244, 553, 643, 833
BccI CCATC 3 cut(s) 65, 137, 371
BceAI ACGGC 1 cut(s) 661
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 4 cut(s) 761, 773, 1077, 1143
BfmI CTRYAG 1 cut(s) 819
BisI GCNGC 5 cut(s) 258, 567, 657, 822, 1209
BlsI GCNGC 5 cut(s) 259, 568, 658, 823, 1210
Bme1390I CCNGG 1 cut(s) 99
BmgT120I GGNCC 2 cut(s) 31, 365
BmiI GGNNCC 4 cut(s) 33, 103, 865, 1107
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 3 cut(s) 142, 439, 520
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 1 cut(s) 562
Bpu14I TTCGAA 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 2 cut(s) 97, 261
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 363, 946
Bse118I RCCGGY 1 cut(s) 197
Bse1I ACTGG 4 cut(s) 784, 991, 1070, 1099
Bse3DI GCAATG 2 cut(s) 222, 552
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 97, 261
BseGI GGATG 4 cut(s) 148, 157, 979, 1036
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 41, 363, 946
BseMI GCAATG 2 cut(s) 222, 552
BseMII CTCAG 5 cut(s) 76, 117, 555, 576, 786
BseNI ACTGG 4 cut(s) 784, 991, 1070, 1099
BseXI GCAGC 4 cut(s) 244, 553, 643, 833
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 3 cut(s) 32, 367, 1056
BshTI ACCGGT 1 cut(s) 197
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 3 cut(s) 41, 363, 946
BsmAI GTCTC 1 cut(s) 1085
BsmI GAATGC 1 cut(s) 814
BsnI GGCC 3 cut(s) 32, 367, 1056
Bsp119I TTCGAA 1 cut(s) 964
Bsp143I GATC 1 cut(s) 1033
BspACI CCGC 4 cut(s) 244, 648, 855, 1209
BspANI GGCC 3 cut(s) 32, 367, 1056
BspCNI CTCAG 5 cut(s) 75, 118, 554, 575, 787
BspLI GGNNCC 4 cut(s) 33, 103, 865, 1107
BspMAI CTGCAG 1 cut(s) 823
BspT104I TTCGAA 1 cut(s) 964
BsrDI GCAATG 2 cut(s) 222, 552
BsrFI RCCGGY 1 cut(s) 197
BsrI ACTGG 4 cut(s) 784, 991, 1070, 1099
BssAI RCCGGY 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 97, 261
BssMI GATC 1 cut(s) 1033
BssNAI GTATAC 1 cut(s) 20
BssSI CACGAG 1 cut(s) 1118
BssT1I CCWWGG 1 cut(s) 261
Bst1107I GTATAC 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 1118
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 809
Bst6I CTCTTC 2 cut(s) 1125, 1161
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBI TTCGAA 1 cut(s) 964
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 6 cut(s) 62, 126, 420, 541, 562, 795
BstEII GGTNACC 1 cut(s) 113
BstF5I GGATG 4 cut(s) 148, 157, 979, 1036
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 1 cut(s) 1036
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 1 cut(s) 1033
BstMWI GCNNNNNNNGC 4 cut(s) 161, 221, 563, 818
BstNI CCWGG 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 97
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 4 cut(s) 244, 553, 643, 833
BstZ17I GTATAC 1 cut(s) 20
BsuRI GGCC 3 cut(s) 32, 367, 1056
BtsCI GGATG 4 cut(s) 148, 157, 979, 1036
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 31, 365
CspAI ACCGGT 1 cut(s) 197
CviAII CATG 1 cut(s) 392
DdeI CTNAG 6 cut(s) 62, 126, 420, 541, 562, 795
DpnI GATC 1 cut(s) 1035
DpnII GATC 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 1125, 1161
EarI CTCTTC 2 cut(s) 1125, 1161
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 1056
Eco32I GATATC 1 cut(s) 768
Eco57I CTGAAG 2 cut(s) 116, 1185
Eco91I GGTNACC 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 113
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 1 cut(s) 395
FatI CATG 1 cut(s) 391
FauNDI CATATG 1 cut(s) 548
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 5 cut(s) 258, 567, 657, 822, 1209
FokI GGATG 4 cut(s) 155, 164, 986, 1043
Fsp4HI GCNGC 5 cut(s) 258, 567, 657, 822, 1209
FspAI RTGCGCAY 1 cut(s) 813
FspBI CTAG 4 cut(s) 761, 773, 1077, 1143
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 1 cut(s) 813
GluI GCNGC 5 cut(s) 258, 567, 657, 822, 1209
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 3 cut(s) 32, 367, 1056
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 814
Hin1II CATG 1 cut(s) 395
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HinfI GANTC 4 cut(s) 58, 122, 159, 1121
HpaI GTTAAC 1 cut(s) 849
HpaII CCGG 1 cut(s) 198
HphI GGTGA 4 cut(s) 158, 520, 698, 1061
Hpy166II GTNNAC 4 cut(s) 20, 518, 849, 1006
Hpy188I TCNGA 9 cut(s) 127, 135, 333, 435, 544, 669, 901, 949, 1165
Hpy188III TCNNGA 1 cut(s) 971
Hpy8I GTNNAC 4 cut(s) 20, 518, 849, 1006
HpyAV CCTTC 5 cut(s) 608, 950, 971, 1046, 1119
HpyCH4III ACNGT 1 cut(s) 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 221, 563, 818
HpyF3I CTNAG 6 cut(s) 62, 126, 420, 541, 562, 795
Hsp92II CATG 1 cut(s) 395
HspAI GCGC 1 cut(s) 812
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 1 cut(s) 1033
LmnI GCTCC 2 cut(s) 483, 1105
Lsp1109I GCAGC 4 cut(s) 244, 553, 643, 833
LweI GCATC 3 cut(s) 142, 439, 520
MaeI CTAG 4 cut(s) 761, 773, 1077, 1143
MaeIII GTNAC 3 cut(s) 113, 208, 691
MalI GATC 1 cut(s) 1035
MboI GATC 1 cut(s) 1033
MboII GAAGA 7 cut(s) 718, 731, 990, 1028, 1033, 1142, 1178
MfeI CAATTG 2 cut(s) 603, 681
MluCI AATT 9 cut(s) 300, 603, 681, 873, 955, 966, 1024, 1084, 1219
MlyI GAGTC 1 cut(s) 1130
MmeI TCCRAC 3 cut(s) 120, 692, 1180
MnlI CCTC 6 cut(s) 80, 142, 293, 499, 571, 1126
Mph1103I ATGCAT 1 cut(s) 554
MseI TTAA 7 cut(s) 50, 270, 633, 848, 983, 1083, 1222
MspA1I CMGCKG 2 cut(s) 566, 855
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 99
MunI CAATTG 2 cut(s) 603, 681
Mva1269I GAATGC 1 cut(s) 814
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 161, 221, 563, 818
NdeI CATATG 1 cut(s) 548
NdeII GATC 1 cut(s) 1033
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 4 cut(s) 33, 103, 865, 1107
NmeAIII GCCGAG 1 cut(s) 764
NmuCI GTSAC 2 cut(s) 208, 691
NsbI TGCGCA 1 cut(s) 813
NsiI ATGCAT 1 cut(s) 554
NspV TTCGAA 1 cut(s) 964
PceI AGGCCT 1 cut(s) 1056
PctI GAATGC 1 cut(s) 814
PfeI GAWTC 3 cut(s) 58, 122, 159
PinAI ACCGGT 1 cut(s) 197
PkrI GCNGC 5 cut(s) 259, 568, 658, 823, 1210
PleI GAGTC 1 cut(s) 1129
PpsI GAGTC 1 cut(s) 1129
Psp6I CCWGG 1 cut(s) 97
PspEI GGTNACC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 4 cut(s) 33, 103, 865, 1107
PspPI GGNCC 2 cut(s) 31, 365
PstI CTGCAG 1 cut(s) 823
PvuII CAGCTG 1 cut(s) 566
SaqAI TTAA 7 cut(s) 50, 270, 633, 848, 983, 1083, 1222
SatI GCNGC 5 cut(s) 258, 567, 657, 822, 1209
Sau3AI GATC 1 cut(s) 1033
Sau96I GGNCC 2 cut(s) 31, 365
SchI GAGTC 1 cut(s) 1130
ScrFI CCNGG 1 cut(s) 99
SfaNI GCATC 3 cut(s) 142, 439, 520
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 964
SgrAI CRCCGGYG 1 cut(s) 197
Sse9I AATT 9 cut(s) 300, 603, 681, 873, 955, 966, 1024, 1084, 1219
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 4 cut(s) 244, 648, 855, 1209
SspMI CTAG 4 cut(s) 761, 773, 1077, 1143
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 809
TaqI TCGA 2 cut(s) 909, 964
TasI AATT 9 cut(s) 300, 603, 681, 873, 955, 966, 1024, 1084, 1219
TauI GCSGC 1 cut(s) 1211
TfiI GAWTC 3 cut(s) 58, 122, 159
Tru1I TTAA 7 cut(s) 50, 270, 633, 848, 983, 1083, 1222
Tru9I TTAA 7 cut(s) 50, 270, 633, 848, 983, 1083, 1222
TscAI CASTG 1 cut(s) 296
TseFI GTSAC 2 cut(s) 208, 691
TseI GCWGC 4 cut(s) 257, 566, 656, 821
Tsp45I GTSAC 2 cut(s) 208, 691
TspDTI ATGAA 2 cut(s) 932, 1143
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 296
XapI RAATTY 3 cut(s) 300, 966, 1024
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 4 cut(s) 761, 773, 1077, 1143
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.