MD08G1105500.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000265
Physical Location & Seq
Reverse (-)
8999343 .. 9001268
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1105500.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGAAAGAAGCGGTAGAGTAGAGCCCCCCACAGTCTTTGGAAAGATAGTCACAATTCTCAGTATCGATGGGGGCGGAATAAGAGGCCTCATTCCGGCAACCATTCTTGCTTTCCTCGAATCTGAACTTCAGAAGCTGGATGGTGAAGATGCAAGAATCGCGGACTATTTTGATGTGATTGCAGGAACAAGCACAGGTGGTCTTGTGACTGCTATGCTCGCAGCCCCAAACGAGAATAACCGCCCACTGTTCGCCGCCAAAGATATTATAGACTTCTACTTTAATCAATGCCCTAAAATCTTCCCCCAAAACACTTGGCCAATTTTTTCTAATACGACAAAGATTATCAAAGCTCTAGCAGGACCAAAATACAATGGCAAGTATTTGCATCGCTTGATTAGGGAAAGGCTGGGCAACAAAAAACTGCACGACACGTTGACTAATGTTGTAATTCCCACATTTGACATCAAGAATCTCCAGCCAGCTATCTTCTCCAACTTTAAGGTGAAAAAGAAGCCATCATACGATGCCTTACTCTCTGACATATGCATTGGAACCTCAGCAGCACCAACTTATTTTCCAGCTCATTATTTTGAAACCAAGAACGCTGCAGGCAGAACTAGGGAATTCAACCTTATAGATGGCGGAATGGCTGCAAATAATCCGACTTCACTTGCTCTTTGTGAAGTTACAAATGAAATAAGGAAGGCGAATCCAGACGTCTTTCCTGTGAAACCTATGGACTATGGGAGATTTCTAGTGATATCATTAGGAACTGGTTCATCAAAAGCTGAAATGAAATACCAAGCACGCTCTGCAGCGAAATGGGGCCTGTTGAATTGGTTAACCAGCGGTGGCTCCACCCCAATCATTAATATATTCAGTCAAGCAGGCGCCGATATGGTTGATCTTCACCTTTCTGCAGCTTTCCAAGCCCTTCATTCTGAAGAAAATTATCTTAGAATTCAGGATGATACCCTTCGTGGGACAATAACTTCTGTGGATAGAGCCACAAAGAAGAATTTGACCAATCTTGTGAAAGTTGGTGAAGGGCTATTGAAAAAACCAGTTTCTCGGGTTAATTTGGACAGTGGCAGGTTTGAGGCTACTAAGCATGAGACAAATGCAGAGGCTCTTACAAGGTTTGCTAAACTACTGTCGGAAGAGAAGAGGAGGCGCCAAGCAGGGTCAACCAACGGGCATGCTGAAAATAATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

407

Amino Acids

44.69

Weight (kDa)

9.32

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 227 5.6e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 723
Acc36I ACCTGC 1 cut(s) 1086
AccB1I GGYRCC 2 cut(s) 893, 1176
AccII CGCG 1 cut(s) 161
AciI CCGC 7 cut(s) 12, 75, 161, 241, 255, 645, 852
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 3 cut(s) 626, 963, 1021
AcuI CTGAAG 2 cut(s) 113, 966
AcyI GRCGYC 3 cut(s) 720, 894, 1177
AfiI CCNNNNNNNGG 2 cut(s) 94, 984
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 4 cut(s) 596, 631, 838, 1060
AluBI AGCT 6 cut(s) 136, 353, 485, 584, 791, 926
AluI AGCT 6 cut(s) 136, 353, 485, 584, 791, 926
Alw26I GTCTC 1 cut(s) 1112
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 1 cut(s) 1074
AoxI GGCC 3 cut(s) 85, 317, 829
ApeKI GCWGC 6 cut(s) 221, 563, 608, 653, 818, 923
ApoI RAATTY 3 cut(s) 626, 963, 1021
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 1 cut(s) 778
AspLEI GCGC 2 cut(s) 896, 1179
AspS9I GGNCC 2 cut(s) 362, 829
AsuHPI GGTGA 4 cut(s) 155, 517, 905, 1058
AvaI CYCGRG 1 cut(s) 1074
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 319
BanI GGYRCC 2 cut(s) 893, 1176
BanII GRGCYC 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbvCI CCTCAGC 1 cut(s) 559
BbvI GCAGC 6 cut(s) 233, 575, 595, 640, 830, 935
BccI CCATC 4 cut(s) 62, 134, 526, 635
BcoDI GTCTC 1 cut(s) 1112
BfaI CTAG 3 cut(s) 356, 621, 758
BfmI CTRYAG 3 cut(s) 609, 816, 921
BfoI RGCGCY 2 cut(s) 897, 1180
BfuAI ACCTGC 1 cut(s) 1086
BisI GCNGC 7 cut(s) 222, 255, 564, 609, 654, 819, 924
BlsI GCNGC 7 cut(s) 223, 256, 565, 610, 655, 820, 925
Bme18I GGWCC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 1074
BmgT120I GGNCC 2 cut(s) 362, 829
BmiI GGNNCC 5 cut(s) 556, 830, 859, 895, 1178
BmsI GCATC 3 cut(s) 139, 397, 517
BpmI CTGGAG 1 cut(s) 461
Bpu10I CCTNAGC 1 cut(s) 559
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 3 cut(s) 720, 894, 1177
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 984
Bse1I ACTGG 2 cut(s) 781, 1067
BseCI ATCGAT 1 cut(s) 66
BseGI GGATG 2 cut(s) 145, 976
BseLI CCNNNNNNNGG 2 cut(s) 94, 984
BseMII CTCAG 2 cut(s) 73, 573
BseNI ACTGG 2 cut(s) 781, 1067
BseRI GAGGAG 1 cut(s) 1186
BseXI GCAGC 6 cut(s) 233, 575, 595, 640, 830, 935
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 3 cut(s) 87, 319, 831
BshNI GGYRCC 2 cut(s) 893, 1176
BshVI ATCGAT 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 1074
BsiSI CCGG 1 cut(s) 95
BslFI GGGAC 1 cut(s) 1000
BslI CCNNNNNNNGG 2 cut(s) 94, 984
BsmAI GTCTC 1 cut(s) 1112
BsmFI GGGAC 1 cut(s) 1000
BsnI GGCC 3 cut(s) 87, 319, 831
BsoBI CYCGRG 1 cut(s) 1074
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 1 cut(s) 907
BspACI CCGC 7 cut(s) 12, 75, 161, 241, 255, 645, 852
BspANI GGCC 3 cut(s) 87, 319, 831
BspCNI CTCAG 2 cut(s) 72, 572
BspDI ATCGAT 1 cut(s) 66
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 5 cut(s) 556, 830, 859, 895, 1178
BspMAI CTGCAG 3 cut(s) 613, 820, 925
BspMI ACCTGC 1 cut(s) 1086
BspT107I GGYRCC 2 cut(s) 893, 1176
BsrI ACTGG 2 cut(s) 781, 1067
BssMI GATC 1 cut(s) 907
BssNI GRCGYC 3 cut(s) 720, 894, 1177
Bst4CI ACNGT 4 cut(s) 34, 249, 1091, 1158
Bst6I CTCTTC 2 cut(s) 1158, 1163
BstACI GRCGYC 3 cut(s) 720, 894, 1177
BstAPI GCANNNNNTGC 1 cut(s) 815
BstC8I GCNNGC 6 cut(s) 219, 483, 613, 811, 892, 1203
BstDEI CTNAG 4 cut(s) 59, 559, 959, 1110
BstF5I GGATG 2 cut(s) 145, 976
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 2 cut(s) 897, 1180
BstHHI GCGC 2 cut(s) 896, 1179
BstKTI GATC 1 cut(s) 910
BstMAI GTCTC 1 cut(s) 1112
BstMBI GATC 1 cut(s) 907
BstMWI GCNNNNNNNGC 4 cut(s) 158, 218, 815, 932
BstNSI RCATGY 1 cut(s) 1205
BstSFI CTRYAG 3 cut(s) 609, 816, 921
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 6 cut(s) 233, 575, 595, 640, 830, 935
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 87, 319, 831
BsuTUI ATCGAT 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 145, 976
BtsIMutI CAGTG 2 cut(s) 245, 1096
BveI ACCTGC 1 cut(s) 1086
Cac8I GCNNGC 6 cut(s) 219, 483, 613, 811, 892, 1203
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 2 cut(s) 896, 1179
Cfr13I GGNCC 2 cut(s) 362, 829
ClaI ATCGAT 1 cut(s) 66
CspCI CAANNNNNGTGG 2 cut(s) 19, 54
CviAII CATG 2 cut(s) 1115, 1202
DdeI CTNAG 4 cut(s) 59, 559, 959, 1110
DinI GGCGCC 2 cut(s) 895, 1178
DpnI GATC 1 cut(s) 909
DpnII GATC 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 2 cut(s) 1158, 1163
EarI CTCTTC 2 cut(s) 1158, 1163
EciI GGCGGA 2 cut(s) 90, 660
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 113, 966
Eco88I CYCGRG 1 cut(s) 1074
EcoO109I RGGNCCY 1 cut(s) 829
EcoRI GAATTC 2 cut(s) 626, 963
EcoRV GATATC 1 cut(s) 765
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 27
EgeI GGCGCC 2 cut(s) 895, 1178
EheI GGCGCC 2 cut(s) 895, 1178
FaeI CATG 2 cut(s) 1118, 1205
FaqI GGGAC 1 cut(s) 1000
FatI CATG 2 cut(s) 1114, 1201
FauNDI CATATG 1 cut(s) 545
Fnu4HI GCNGC 7 cut(s) 222, 255, 564, 609, 654, 819, 924
FokI GGATG 2 cut(s) 152, 983
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 7 cut(s) 222, 255, 564, 609, 654, 819, 924
FspBI CTAG 3 cut(s) 356, 621, 758
GlaI GCGC 2 cut(s) 895, 1178
GluI GCNGC 7 cut(s) 222, 255, 564, 609, 654, 819, 924
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 461
HaeII RGCGCY 2 cut(s) 897, 1180
HaeIII GGCC 3 cut(s) 87, 319, 831
HapII CCGG 1 cut(s) 95
HhaI GCGC 2 cut(s) 896, 1179
Hin1I GRCGYC 3 cut(s) 720, 894, 1177
Hin1II CATG 2 cut(s) 1118, 1205
Hin6I GCGC 2 cut(s) 894, 1177
HinP1I GCGC 2 cut(s) 894, 1177
HincII GTYRAC 3 cut(s) 438, 846, 1191
HindII GTYRAC 3 cut(s) 438, 846, 1191
HinfI GANTC 4 cut(s) 119, 156, 472, 712
HpaI GTTAAC 1 cut(s) 846
HpaII CCGG 1 cut(s) 95
HphI GGTGA 4 cut(s) 155, 517, 905, 1058
Hpy166II GTNNAC 3 cut(s) 438, 846, 1191
Hpy188I TCNGA 6 cut(s) 124, 132, 541, 666, 946, 1162
Hpy188III TCNNGA 3 cut(s) 469, 716, 968
Hpy8I GTNNAC 3 cut(s) 438, 846, 1191
HpyAV CCTTC 4 cut(s) 700, 947, 989, 1043
HpyCH4III ACNGT 4 cut(s) 34, 249, 1091, 1158
HpyCH4IV ACGT 2 cut(s) 434, 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 218, 815, 932
HpyF3I CTNAG 4 cut(s) 59, 559, 959, 1110
HpySE526I ACGT 2 cut(s) 434, 720
Hsp92I GRCGYC 3 cut(s) 720, 894, 1177
Hsp92II CATG 2 cut(s) 1118, 1205
HspAI GCGC 2 cut(s) 894, 1177
KasI GGCGCC 2 cut(s) 893, 1176
KspAI GTTAAC 1 cut(s) 846
Kzo9I GATC 1 cut(s) 907
LmnI GCTCC 1 cut(s) 863
Lsp1109I GCAGC 6 cut(s) 233, 575, 595, 640, 830, 935
LweI GCATC 3 cut(s) 139, 397, 517
MaeI CTAG 3 cut(s) 356, 621, 758
MaeII ACGT 2 cut(s) 434, 720
MaeIII GTNAC 3 cut(s) 49, 205, 688
MalI GATC 1 cut(s) 909
MboI GATC 1 cut(s) 907
MboII GAAGA 8 cut(s) 158, 292, 481, 902, 959, 1030, 1175, 1180
MhlI GDGCHC 1 cut(s) 27
MlsI TGGCCA 1 cut(s) 319
MluNI TGGCCA 1 cut(s) 319
Mly113I GGCGCC 2 cut(s) 894, 1177
MmeI TCCRAC 3 cut(s) 519, 689, 1140
MnlI CCTC 8 cut(s) 77, 98, 125, 568, 1096, 1123, 1164, 1167
Mox20I TGGCCA 1 cut(s) 319
Mph1103I ATGCAT 1 cut(s) 551
MroXI GAANNNNTTC 1 cut(s) 778
MscI TGGCCA 1 cut(s) 319
MseI TTAA 5 cut(s) 282, 501, 845, 873, 1080
Msp20I TGGCCA 1 cut(s) 319
MspA1I CMGCKG 1 cut(s) 852
MspI CCGG 1 cut(s) 95
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 4 cut(s) 158, 218, 815, 932
NarI GGCGCC 2 cut(s) 894, 1177
NdeI CATATG 1 cut(s) 545
NdeII GATC 1 cut(s) 907
NlaIII CATG 2 cut(s) 1118, 1205
NlaIV GGNNCC 5 cut(s) 556, 830, 859, 895, 1178
NmuCI GTSAC 2 cut(s) 49, 205
NsiI ATGCAT 1 cut(s) 551
NspI RCATGY 1 cut(s) 1205
PaeI GCATGC 1 cut(s) 1205
PceI AGGCCT 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 4 cut(s) 119, 156, 472, 712
PkrI GCNGC 7 cut(s) 223, 256, 565, 610, 655, 820, 925
PluTI GGCGCC 2 cut(s) 897, 1180
PshBI ATTAAT 1 cut(s) 873
PspFI CCCAGC 1 cut(s) 409
PspN4I GGNNCC 5 cut(s) 556, 830, 859, 895, 1178
PspPI GGNCC 2 cut(s) 362, 829
PstI CTGCAG 3 cut(s) 613, 820, 925
PstNI CAGNNNCTG 1 cut(s) 136
SaqAI TTAA 5 cut(s) 282, 501, 845, 873, 1080
SatI GCNGC 7 cut(s) 222, 255, 564, 609, 654, 819, 924
Sau3AI GATC 1 cut(s) 907
Sau96I GGNCC 2 cut(s) 362, 829
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 3 cut(s) 139, 397, 517
SfcI CTRYAG 3 cut(s) 609, 816, 921
SfoI GGCGCC 2 cut(s) 895, 1178
SinI GGWCC 1 cut(s) 362
SphI GCATGC 1 cut(s) 1205
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 7 cut(s) 12, 75, 161, 241, 255, 645, 852
SspDI GGCGCC 2 cut(s) 893, 1176
SspMI CTAG 3 cut(s) 356, 621, 758
StuI AGGCCT 1 cut(s) 87
TaaI ACNGT 4 cut(s) 34, 249, 1091, 1158
TaiI ACGT 2 cut(s) 437, 723
TaqI TCGA 2 cut(s) 66, 117
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 4 cut(s) 119, 156, 472, 712
Tru1I TTAA 5 cut(s) 282, 501, 845, 873, 1080
Tru9I TTAA 5 cut(s) 282, 501, 845, 873, 1080
TscAI CASTG 2 cut(s) 252, 1096
TseFI GTSAC 2 cut(s) 49, 205
TseI GCWGC 6 cut(s) 221, 563, 608, 653, 818, 923
Tsp45I GTSAC 2 cut(s) 49, 205
TspDTI ATGAA 4 cut(s) 711, 771, 812, 929
TspRI CASTG 2 cut(s) 252, 1096
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 3 cut(s) 626, 963, 1021
XceI RCATGY 1 cut(s) 1205
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 3 cut(s) 356, 621, 758
ZraI GACGTC 1 cut(s) 721
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.