Rroxscaffold_7G00161040

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000265
Physical Location & Seq
Forward (+)
3872678 .. 3875842
3165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161040.1

Sequence Viewer

Length: 1164 bp
ATGGAGATGCCTATCCACGGATCCGCTTCTCTTCCAAATAATTTCATCAAGAGAGAAAGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATCAGAGGAATTATCCCTGGTACTCTTCTCAGTTTCCTTGAGTCTGAGCTTCAGAAGCTGGATGGTGAGGATGCAAGACTGGCAGATTATTTTGATGTCATTGCAGGAACTAGTACTGGTGGCCTTGTGACTGCCATGCTTACCGCCCCGGATGAAAACGGCCGTCCATTATTTGCTGCTAAAGATATCAACAAATTTTACCTTGAACACTGCCCTAAGATATTTCCCCAAGATAGTTGCAAAATTGATTGTCTTCCATTAACTCCAATAACTGATGCGGTGGCGGCGGTAAAAGCTTTCAGAGGGCCAAAGTATGATGGGGAGTATTTACATAATATAGTGAAGGAAAAGTTAGGAGACATAAGATTGCATCAGACATTGACCAACGTCATAATTCCCACGTTTGATATAAAGAGGCTCCAACCAACCGTCTTCTCCAGCTATGAGGCTTTGGTGGCTATAAGTGAAGTGTCAAAAGAAATTTACAAGGGAAATCCCGACTTTTTTACAATGAAGGCAACTGAATACGCCCGGTTTCTAGTTGTATCATTGGGTACTGGTTCAGCAAAATGTGATGAGAAGTACGATGCCCAAAATGCAGCTAAGTGGGGTATTTTGGGATGGTTGATCGGGGGCGGTTCCTGTCCGTTGGTTGATATATTTACTCAAGCAAGTAGTGATATGGTTGACTTCCACCTTTCTACAGTGTTCCAAGCCCTTCACTCTGAGAAAAATTACCTAAGAATCCAGGATGATGATTTGGGTAAGACATTGTGTTCTGTGGATATTGCTACAGAAGAAAACTTGCAAAATCTTGTAAAAGTTGGAGAAAATTTGTTAAAGAAACCAGTTTCCCGAGTAAACTTGGAAACCGGTATCCATGAGCCTGCCCATCATTCTACCAACGAAGAGGCTCTAGTAAGGGTAGCTGGAATTTTGTCCAAGGAAAGGAGAGTCCGTGAAATGAGATCGCCACAGGAAAGAAGGCCACAACAACAACCAGCTCCAACTTGTCATTCTCCTTCATCAATGTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.77

Weight (kDa)

5.85

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 35 - 179 2.6e-12 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 24, 267, 401, 407, 410, 759
AclWI GGATC 2 cut(s) 15, 28
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 4 cut(s) 317, 603, 955, 1056
AcuI CTGAAG 2 cut(s) 53, 158
AfaI GTAC 4 cut(s) 145, 238, 679, 707
AfiI CCNNNNNNNGG 3 cut(s) 17, 83, 1071
AgeI ACCGGT 1 cut(s) 995
AgsI TTSAA 1 cut(s) 329
AhlI ACTAGT 1 cut(s) 233
AjnI CCWGG 2 cut(s) 139, 870
AjuI GAANNNNNNNTTGG 2 cut(s) 1123, 1155
AluBI AGCT 7 cut(s) 172, 181, 419, 564, 725, 1052, 1127
AluI AGCT 7 cut(s) 172, 181, 419, 564, 725, 1052, 1127
Alw26I GTCTC 2 cut(s) 78, 474
AlwI GGATC 2 cut(s) 15, 28
AlwNI CAGNNNCTG 1 cut(s) 181
Ama87I CYCGRG 1 cut(s) 978
AoxI GGCC 4 cut(s) 244, 283, 428, 1109
ApeKI GCWGC 2 cut(s) 299, 722
ApoI RAATTY 4 cut(s) 317, 603, 955, 1056
AsiGI ACCGGT 1 cut(s) 995
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 2 cut(s) 272, 655
AsuHPI GGTGA 1 cut(s) 200
AvaI CYCGRG 1 cut(s) 978
BamHI GGATCC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 368, 547
BbvI GCAGC 2 cut(s) 286, 734
BccI CCATC 5 cut(s) 107, 179, 434, 738, 1023
BceAI ACGGC 2 cut(s) 270, 298
BciT130I CCWGG 2 cut(s) 141, 872
BciVI GTATCC 1 cut(s) 1010
BcnI CCSGG 2 cut(s) 272, 655
BcoDI GTCTC 2 cut(s) 78, 474
BcuI ACTAGT 1 cut(s) 233
BfaI CTAG 3 cut(s) 234, 662, 1040
BfmI CTRYAG 2 cut(s) 825, 915
BfuI GTATCC 1 cut(s) 1010
BisI GCNGC 3 cut(s) 300, 408, 723
BlsI GCNGC 3 cut(s) 301, 409, 724
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 4 cut(s) 141, 272, 655, 872
BmeT110I CYCGRG 1 cut(s) 978
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 3 cut(s) 22, 542, 763
BmrFI CCNGG 4 cut(s) 141, 272, 655, 872
BmsI GCATC 4 cut(s) 184, 388, 502, 700
BoxI GACNNNNGTC 1 cut(s) 509
BpiI GAAGAC 2 cut(s) 368, 547
BpmI CTGGAG 1 cut(s) 544
BpuEI CTTGAG 2 cut(s) 182, 774
BpuMI CCSGG 2 cut(s) 272, 655
BsaBI GATNNNNATC 1 cut(s) 11
BsaJI CCNNGG 4 cut(s) 16, 139, 270, 1065
BsaWI WCCGGW 1 cut(s) 995
BsaXI ACNNNNNCTCC 2 cut(s) 1066, 1096
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 83, 1071
Bse118I RCCGGY 1 cut(s) 995
Bse1I ACTGG 4 cut(s) 207, 244, 685, 971
Bse3DI GCAATG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 2 cut(s) 141, 872
BseDI CCNNGG 4 cut(s) 16, 139, 270, 1065
BseGI GGATG 5 cut(s) 190, 199, 280, 749, 880
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 3 cut(s) 17, 83, 1071
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 3 cut(s) 159, 166, 840
BseNI ACTGG 4 cut(s) 207, 244, 685, 971
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 286, 734
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 4 cut(s) 246, 285, 430, 1111
BshTI ACCGGT 1 cut(s) 995
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 978
BsiSI CCGG 3 cut(s) 272, 655, 996
BslI CCNNNNNNNGG 3 cut(s) 17, 83, 1071
BsmAI GTCTC 2 cut(s) 78, 474
BsnI GGCC 4 cut(s) 246, 285, 430, 1111
BsoBI CYCGRG 1 cut(s) 978
Bsp143I GATC 4 cut(s) 20, 59, 750, 1091
BspACI CCGC 6 cut(s) 24, 267, 401, 407, 410, 759
BspANI GGCC 4 cut(s) 246, 285, 430, 1111
BspCNI CTCAG 3 cut(s) 160, 165, 841
BspLI GGNNCC 3 cut(s) 22, 542, 763
BspPI GGATC 2 cut(s) 15, 28
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 995
BsrI ACTGG 4 cut(s) 207, 244, 685, 971
BssAI RCCGGY 1 cut(s) 995
BssECI CCNNGG 4 cut(s) 16, 139, 270, 1065
BssMI GATC 4 cut(s) 20, 59, 750, 1091
BssT1I CCWWGG 1 cut(s) 1065
Bst2UI CCWGG 2 cut(s) 141, 872
Bst4CI ACNGT 3 cut(s) 100, 553, 829
Bst6I CTCTTC 3 cut(s) 36, 153, 1026
BstC8I GCNNGC 1 cut(s) 1011
BstDEI CTNAG 7 cut(s) 104, 152, 168, 339, 726, 849, 863
BstDSI CCRYGG 1 cut(s) 16
BstF5I GGATG 5 cut(s) 190, 199, 280, 749, 880
BstKTI GATC 4 cut(s) 23, 62, 753, 1094
BstMAI GTCTC 2 cut(s) 78, 474
BstMBI GATC 4 cut(s) 20, 59, 750, 1091
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 6 cut(s) 178, 203, 407, 416, 578, 719
BstNI CCWGG 2 cut(s) 141, 872
BstPAI GACNNNNGTC 1 cut(s) 509
BstSCI CCNGG 4 cut(s) 139, 270, 653, 870
BstSFI CTRYAG 2 cut(s) 825, 915
BstV1I GCAGC 2 cut(s) 286, 734
BstV2I GAAGAC 2 cut(s) 368, 547
BstX2I RGATCY 1 cut(s) 20
BstYI RGATCY 1 cut(s) 20
BstZI CGGCCG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 1010
BsuRI GGCC 4 cut(s) 246, 285, 430, 1111
BtgI CCRYGG 1 cut(s) 16
BtsCI GGATG 5 cut(s) 190, 199, 280, 749, 880
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 96, 331, 834
Cac8I GCNNGC 1 cut(s) 1011
CaiI CAGNNNCTG 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 995
Cfr13I GGNCC 1 cut(s) 428
Csp6I GTAC 4 cut(s) 144, 237, 678, 706
CspAI ACCGGT 1 cut(s) 995
CviAII CATG 2 cut(s) 259, 1004
CviQI GTAC 4 cut(s) 144, 237, 678, 706
DdeI CTNAG 7 cut(s) 104, 152, 168, 339, 726, 849, 863
DpnI GATC 4 cut(s) 22, 61, 752, 1093
DpnII GATC 4 cut(s) 20, 59, 750, 1091
EaeI YGGCCR 1 cut(s) 283
EagI CGGCCG 1 cut(s) 283
Eam1104I CTCTTC 3 cut(s) 36, 153, 1026
EarI CTCTTC 3 cut(s) 36, 153, 1026
EclXI CGGCCG 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 1065
Eco32I GATATC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 53, 158
Eco88I CYCGRG 1 cut(s) 978
EcoRII CCWGG 2 cut(s) 139, 870
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 1065
ErhI CCWWGG 1 cut(s) 1065
FaeI CATG 2 cut(s) 262, 1007
FatI CATG 2 cut(s) 258, 1003
Fnu4HI GCNGC 3 cut(s) 300, 408, 723
FokI GGATG 5 cut(s) 197, 206, 287, 756, 887
Fsp4HI GCNGC 3 cut(s) 300, 408, 723
FspBI CTAG 3 cut(s) 234, 662, 1040
GluI GCNGC 3 cut(s) 300, 408, 723
GsuI CTGGAG 1 cut(s) 544
HaeIII GGCC 4 cut(s) 246, 285, 430, 1111
HapII CCGG 3 cut(s) 272, 655, 996
Hin1II CATG 2 cut(s) 262, 1007
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 4 cut(s) 123, 164, 867, 1077
HpaII CCGG 3 cut(s) 272, 655, 996
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 811, 985
Hpy188I TCNGA 6 cut(s) 128, 169, 177, 425, 498, 850
Hpy188III TCNNGA 3 cut(s) 49, 620, 978
Hpy8I GTNNAC 2 cut(s) 811, 985
HpyAV CCTTC 6 cut(s) 77, 460, 631, 851, 1101, 1155
HpyCH4III ACNGT 3 cut(s) 100, 553, 829
HpyCH4IV ACGT 2 cut(s) 510, 524
HpyCH4V TGCA 6 cut(s) 197, 227, 363, 493, 722, 931
HpyF10VI GCNNNNNNNGC 6 cut(s) 178, 203, 407, 416, 578, 719
HpyF3I CTNAG 7 cut(s) 104, 152, 168, 339, 726, 849, 863
HpySE526I ACGT 2 cut(s) 510, 524
Hsp92II CATG 2 cut(s) 262, 1007
Kzo9I GATC 4 cut(s) 20, 59, 750, 1091
LmnI GCTCC 2 cut(s) 546, 1132
Lsp1109I GCAGC 2 cut(s) 286, 734
LweI GCATC 4 cut(s) 184, 388, 502, 700
MaeI CTAG 3 cut(s) 234, 662, 1040
MaeII ACGT 2 cut(s) 510, 524
MaeIII GTNAC 2 cut(s) 94, 250
MalI GATC 4 cut(s) 22, 61, 752, 1093
MboI GATC 4 cut(s) 20, 59, 750, 1091
MboII GAAGA 6 cut(s) 23, 140, 368, 547, 932, 1043
MflI RGATCY 1 cut(s) 20
MlyI GAGTC 2 cut(s) 173, 1086
MmeI TCCRAC 3 cut(s) 568, 928, 1154
MnlI CCTC 8 cut(s) 101, 110, 122, 184, 419, 531, 562, 1027
MseI TTAA 4 cut(s) 383, 962, 1158, 1162
MspI CCGG 3 cut(s) 272, 655, 996
MspR9I CCNGG 4 cut(s) 141, 272, 655, 872
MvaI CCWGG 2 cut(s) 141, 872
MwoI GCNNNNNNNGC 6 cut(s) 178, 203, 407, 416, 578, 719
NciI CCSGG 2 cut(s) 272, 655
NdeII GATC 4 cut(s) 20, 59, 750, 1091
NlaIII CATG 2 cut(s) 262, 1007
NlaIV GGNNCC 3 cut(s) 22, 542, 763
NmuCI GTSAC 2 cut(s) 94, 250
PacI TTAATTAA 1 cut(s) 1162
PfeI GAWTC 2 cut(s) 123, 867
PfoI TCCNGGA 1 cut(s) 870
PinAI ACCGGT 1 cut(s) 995
PkrI GCNGC 3 cut(s) 301, 409, 724
PleI GAGTC 2 cut(s) 172, 1085
PpsI GAGTC 2 cut(s) 172, 1085
PshAI GACNNNNGTC 1 cut(s) 509
Psp6I CCWGG 2 cut(s) 139, 870
PspGI CCWGG 2 cut(s) 139, 870
PspN4I GGNNCC 3 cut(s) 22, 542, 763
PspPI GGNCC 1 cut(s) 428
PstNI CAGNNNCTG 1 cut(s) 181
PsuI RGATCY 1 cut(s) 20
RsaI GTAC 4 cut(s) 145, 238, 679, 707
RsaNI GTAC 4 cut(s) 144, 237, 678, 706
SaqAI TTAA 4 cut(s) 383, 962, 1158, 1162
SatI GCNGC 3 cut(s) 300, 408, 723
Sau3AI GATC 4 cut(s) 20, 59, 750, 1091
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 238
SchI GAGTC 2 cut(s) 173, 1086
ScrFI CCNGG 4 cut(s) 141, 272, 655, 872
SfaNI GCATC 4 cut(s) 184, 388, 502, 700
SfcI CTRYAG 2 cut(s) 825, 915
SmlI CTYRAG 2 cut(s) 161, 789
SmoI CTYRAG 2 cut(s) 161, 789
SpeI ACTAGT 1 cut(s) 233
SsiI CCGC 6 cut(s) 24, 267, 401, 407, 410, 759
SspMI CTAG 3 cut(s) 234, 662, 1040
StyD4I CCNGG 4 cut(s) 139, 270, 653, 870
StyI CCWWGG 1 cut(s) 1065
TaaI ACNGT 3 cut(s) 100, 553, 829
TaiI ACGT 2 cut(s) 513, 527
TatI WGTACW 1 cut(s) 236
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 2 cut(s) 123, 867
Tru1I TTAA 4 cut(s) 383, 962, 1158, 1162
Tru9I TTAA 4 cut(s) 383, 962, 1158, 1162
TscAI CASTG 3 cut(s) 103, 338, 834
TseFI GTSAC 2 cut(s) 94, 250
TseI GCWGC 2 cut(s) 299, 722
Tsp45I GTSAC 2 cut(s) 94, 250
TspDTI ATGAA 4 cut(s) 34, 291, 650, 1137
TspGWI ACGGA 3 cut(s) 33, 759, 1070
TspRI CASTG 3 cut(s) 103, 338, 834
XapI RAATTY 4 cut(s) 317, 603, 955, 1056
XspI CTAG 3 cut(s) 234, 662, 1040
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.