Lipolytic acyl hydrolase (LAH)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 153 |
| AclWI | GGATC | 2 cut(s) | 12, 258 |
| AcsI | RAATTY | 1 cut(s) | 222 |
| AcuI | CTGAAG | 1 cut(s) | 261 |
| AdeI | CACNNNGTG | 1 cut(s) | 263 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 81, 202 |
| AgsI | TTSAA | 1 cut(s) | 190 |
| AjnI | CCWGG | 1 cut(s) | 351 |
| AluBI | AGCT | 1 cut(s) | 47 |
| AluI | AGCT | 1 cut(s) | 47 |
| Alw26I | GTCTC | 1 cut(s) | 332 |
| AlwI | GGATC | 2 cut(s) | 12, 258 |
| ApoI | RAATTY | 1 cut(s) | 222 |
| AspS9I | GGNCC | 1 cut(s) | 307 |
| AsuHPI | GGTGA | 1 cut(s) | 28 |
| AsuII | TTCGAA | 1 cut(s) | 220 |
| AvaII | GGWCC | 1 cut(s) | 307 |
| AxyI | CCTNAGG | 1 cut(s) | 25 |
| BccI | CCATC | 2 cut(s) | 224, 318 |
| BciT130I | CCWGG | 1 cut(s) | 353 |
| BclI | TGATCA | 1 cut(s) | 126 |
| BcoDI | GTCTC | 1 cut(s) | 332 |
| BisI | GCNGC | 1 cut(s) | 153 |
| BlsI | GCNGC | 1 cut(s) | 154 |
| Bme1390I | CCNGG | 1 cut(s) | 353 |
| Bme18I | GGWCC | 1 cut(s) | 307 |
| BmgT120I | GGNCC | 1 cut(s) | 307 |
| BmiI | GGNNCC | 1 cut(s) | 115 |
| BmrFI | CCNGG | 1 cut(s) | 353 |
| BmsI | GCATC | 1 cut(s) | 181 |
| Bpu14I | TTCGAA | 1 cut(s) | 220 |
| BsaBI | GATNNNNATC | 1 cut(s) | 171 |
| BsaJI | CCNNGG | 1 cut(s) | 352 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 81, 202 |
| Bse1I | ACTGG | 2 cut(s) | 37, 345 |
| Bse21I | CCTNAGG | 1 cut(s) | 25 |
| Bse8I | GATNNNNATC | 1 cut(s) | 171 |
| BseBI | CCWGG | 1 cut(s) | 353 |
| BseDI | CCNNGG | 1 cut(s) | 352 |
| BseGI | GGATG | 1 cut(s) | 235 |
| BseJI | GATNNNNATC | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 81, 202 |
| BseMII | CTCAG | 1 cut(s) | 331 |
| BseNI | ACTGG | 2 cut(s) | 37, 345 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 81, 202 |
| BsmAI | GTCTC | 1 cut(s) | 332 |
| Bsp119I | TTCGAA | 1 cut(s) | 220 |
| Bsp143I | GATC | 3 cut(s) | 4, 126, 250 |
| BspACI | CCGC | 1 cut(s) | 153 |
| BspCNI | CTCAG | 1 cut(s) | 330 |
| BspLI | GGNNCC | 1 cut(s) | 115 |
| BspPI | GGATC | 2 cut(s) | 12, 258 |
| BspT104I | TTCGAA | 1 cut(s) | 220 |
| BsrI | ACTGG | 2 cut(s) | 37, 345 |
| BssECI | CCNNGG | 1 cut(s) | 352 |
| BssMI | GATC | 3 cut(s) | 4, 126, 250 |
| Bst2UI | CCWGG | 1 cut(s) | 353 |
| BstBI | TTCGAA | 1 cut(s) | 220 |
| BstDEI | CTNAG | 3 cut(s) | 25, 260, 317 |
| BstF5I | GGATG | 1 cut(s) | 235 |
| BstKTI | GATC | 3 cut(s) | 7, 129, 253 |
| BstMAI | GTCTC | 1 cut(s) | 332 |
| BstMBI | GATC | 3 cut(s) | 4, 126, 250 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 71, 152 |
| BstNI | CCWGG | 1 cut(s) | 353 |
| BstSCI | CCNGG | 1 cut(s) | 351 |
| Bsu36I | CCTNAGG | 1 cut(s) | 25 |
| BtsCI | GGATG | 1 cut(s) | 235 |
| Cfr13I | GGNCC | 1 cut(s) | 307 |
| CviAII | CATG | 1 cut(s) | 68 |
| CviJI | RGCY | 3 cut(s) | 36, 47, 194 |
| CviKI_1 | RGCY | 3 cut(s) | 36, 47, 194 |
| DdeI | CTNAG | 3 cut(s) | 25, 260, 317 |
| DpnI | GATC | 3 cut(s) | 6, 128, 252 |
| DpnII | GATC | 3 cut(s) | 4, 126, 250 |
| DraIII | CACNNNGTG | 1 cut(s) | 263 |
| Eco32I | GATATC | 1 cut(s) | 21 |
| Eco47I | GGWCC | 1 cut(s) | 307 |
| Eco57I | CTGAAG | 1 cut(s) | 261 |
| Eco81I | CCTNAGG | 1 cut(s) | 25 |
| EcoRI | GAATTC | 1 cut(s) | 222 |
| EcoRII | CCWGG | 1 cut(s) | 351 |
| EcoRV | GATATC | 1 cut(s) | 21 |
| FaeI | CATG | 1 cut(s) | 71 |
| FaiI | YATR | 4 cut(s) | 14, 69, 161, 287 |
| FatI | CATG | 1 cut(s) | 67 |
| FbaI | TGATCA | 1 cut(s) | 126 |
| Fnu4HI | GCNGC | 1 cut(s) | 153 |
| FokI | GGATG | 1 cut(s) | 242 |
| Fsp4HI | GCNGC | 1 cut(s) | 153 |
| GluI | GCNGC | 1 cut(s) | 153 |
| Hin1II | CATG | 1 cut(s) | 71 |
| HincII | GTYRAC | 1 cut(s) | 102 |
| HindII | GTYRAC | 1 cut(s) | 102 |
| HinfI | GANTC | 1 cut(s) | 301 |
| HpaI | GTTAAC | 1 cut(s) | 102 |
| HphI | GGTGA | 1 cut(s) | 28 |
| Hpy166II | GTNNAC | 1 cut(s) | 102 |
| Hpy188I | TCNGA | 3 cut(s) | 9, 205, 320 |
| Hpy188III | TCNNGA | 2 cut(s) | 227, 305 |
| Hpy8I | GTNNAC | 1 cut(s) | 102 |
| HpyAV | CCTTC | 1 cut(s) | 206 |
| HpyCH4V | TGCA | 2 cut(s) | 172, 247 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 71, 152 |
| HpyF3I | CTNAG | 3 cut(s) | 25, 260, 317 |
| Hsp92II | CATG | 1 cut(s) | 71 |
| Ksp22I | TGATCA | 1 cut(s) | 126 |
| KspAI | GTTAAC | 1 cut(s) | 102 |
| Kzo9I | GATC | 3 cut(s) | 4, 126, 250 |
| LpnPI | CCDG | 5 cut(s) | 18, 212, 233, 290, 338 |
| LweI | GCATC | 1 cut(s) | 181 |
| MalI | GATC | 3 cut(s) | 6, 128, 252 |
| MboI | GATC | 3 cut(s) | 4, 126, 250 |
| MboII | GAAGA | 4 cut(s) | 209, 267, 305, 310 |
| MluCI | AATT | 2 cut(s) | 94, 222 |
| MnlI | CCTC | 3 cut(s) | 85, 130, 321 |
| MseI | TTAA | 2 cut(s) | 101, 358 |
| MspR9I | CCNGG | 1 cut(s) | 353 |
| MvaI | CCWGG | 1 cut(s) | 353 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 71, 152 |
| NdeII | GATC | 3 cut(s) | 4, 126, 250 |
| NlaIII | CATG | 1 cut(s) | 71 |
| NlaIV | GGNNCC | 1 cut(s) | 115 |
| NspV | TTCGAA | 1 cut(s) | 220 |
| PfeI | GAWTC | 1 cut(s) | 301 |
| PkrI | GCNGC | 1 cut(s) | 154 |
| Psp6I | CCWGG | 1 cut(s) | 351 |
| PspGI | CCWGG | 1 cut(s) | 351 |
| PspN4I | GGNNCC | 1 cut(s) | 115 |
| PspPI | GGNCC | 1 cut(s) | 307 |
| SaqAI | TTAA | 2 cut(s) | 101, 358 |
| SatI | GCNGC | 1 cut(s) | 153 |
| Sau3AI | GATC | 3 cut(s) | 4, 126, 250 |
| Sau96I | GGNCC | 1 cut(s) | 307 |
| ScrFI | CCNGG | 1 cut(s) | 353 |
| SetI | ASST | 2 cut(s) | 49, 122 |
| SfaNI | GCATC | 1 cut(s) | 181 |
| SfuI | TTCGAA | 1 cut(s) | 220 |
| SgeI | CNNG | 9 cut(s) | 45, 80, 117, 155, 159, 214, 239, 260, 317 |
| SinI | GGWCC | 1 cut(s) | 307 |
| Sse9I | AATT | 2 cut(s) | 94, 222 |
| SsiI | CCGC | 1 cut(s) | 153 |
| StyD4I | CCNGG | 1 cut(s) | 351 |
| TaqI | TCGA | 3 cut(s) | 41, 165, 220 |
| TasI | AATT | 2 cut(s) | 94, 222 |
| TauI | GCSGC | 1 cut(s) | 155 |
| TfiI | GAWTC | 1 cut(s) | 301 |
| Tru1I | TTAA | 2 cut(s) | 101, 358 |
| Tru9I | TTAA | 2 cut(s) | 101, 358 |
| VpaK11BI | GGWCC | 1 cut(s) | 307 |
| XapI | RAATTY | 1 cut(s) | 222 |