RchiOBHm_Chr6g0307391

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000265
Physical Location & Seq
Reverse (-)
65806183 .. 65809183
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27635

Sequence Viewer

Length: 1236 bp
ATGGAGAGAGCAAGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATAAGAGGGATTATCCCCGGAACTATGCTCAGCTTCTTAGAGTCCGAGCTTCAGAAGCTGGATGGTGAAGATGCAAGACTAGCAGATTATTTTGATGTAATTGCAGGGACCAGCACTGGTGGTCTTGTCACTGCTATGCTTACTGCCCCAGATGAAAATAACCGTCCAATATTTGCTGCTAAAGATATTAACGATTTTTATCTTGAACACTGTCCTAAAATCTTCCCCCAAGACAGCACCTCAATAATTGGTGAAGCTGTAGAAAAGTTGAAAGTTGTGAGAGGGCCAAAATATGATGGGGAGTATTTACATAAGATAGTCAGGGAAAAGTTGGGAGACATACGATTGAACGAGACATTGACTAACGTTGTAATCCCAACTTTTGATATAAAGAGGCTTCAGCCAGTCATCTTCTCCAGCTATGAGGTTAAGAAGAATGATAGCTTAGATGCTTTACTCTCCGACATATGCATCGGAACTTCAGCAGCCCCAACTTATCTCCCAGCGCATCATTTTGAAACCCAAAATTCCAGAACTGGAGAAGCAAGAGAATTCAATCTGATTGACGGTGCAGTCGCTGCTAATAATCCGGCTTTGGTCGCTATAAACGAAGTAACAAAAGAAATCCACCAAGGAAACACCGACTTCTTTCCTATGAGGCCAACTGAGTATGGGCGATTCCTAGTTATATCACTGGGTACTGGTTCGGGGAAATGCGAGGAGAAGTATGCCGCCGCAAATGCTGCTAAGTGGGGCGTTTTGGGGTGGCTAACCGCAGGTGGTTCATGTCCATTGGTTGACGTATTTACTCAAGCAAGTAGTGACATGGTCGACTTCCACCTAGCCACCGTTTTCCAAGCTCTTCATTCTGAGAAAAATTACCTTCGGATTCAAGACGATTCACTGGAGGGAACAGTGTCTTCAGTGGACATTGCTACGCATGAGAACTTGAACAACCTTGTAAAAGTTGGAGAGGAGTTGTTAAAGAAACCGGTTTCAAGGGTGAATATGGAAACTGGTATATATGAGCCTGCCCATCAGTCCACAAATGAAGAGGCTCTCGTAAGGGTAGCGGGAATTCTGTCTAAAGAGAGGAGAGTTCGTGAAATGAGATCACCACATGGGAAGGCCGAAGGCTGCAGGCGCAGTTACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

411

Amino Acids

45.21

Weight (kDa)

5.41

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 20 - 228 9.3e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 845
AasI GACNNNNNNGTC 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 845
AccI GTMKAC 1 cut(s) 909
AciI CCGC 4 cut(s) 810, 813, 852, 1151
AclI AACGTT 1 cut(s) 444
AcsI RAATTY 3 cut(s) 604, 629, 1155
AcuI CTGAAG 5 cut(s) 8, 113, 461, 543, 984
AfaI GTAC 1 cut(s) 778
AfiI CCNNNNNNNGG 1 cut(s) 38
AgeI ACCGGT 1 cut(s) 1069
AgsI TTSAA 8 cut(s) 284, 349, 427, 596, 634, 971, 1030, 1077
AluBI AGCT 7 cut(s) 111, 127, 136, 335, 498, 522, 938
AluI AGCT 7 cut(s) 111, 127, 136, 335, 498, 522, 938
Alw26I GTCTC 3 cut(s) 33, 408, 425
AlwNI CAGNNNCTG 2 cut(s) 136, 656
AoxI GGCC 3 cut(s) 362, 737, 1206
ApeKI GCWGC 5 cut(s) 254, 563, 656, 821, 1215
ApoI RAATTY 3 cut(s) 604, 629, 1155
AsiGI ACCGGT 1 cut(s) 1069
AspLEI GCGC 2 cut(s) 586, 1224
AspS9I GGNCC 2 cut(s) 186, 362
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 4 cut(s) 155, 341, 1093, 1185
AvaII GGWCC 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 990
BbvI GCAGC 5 cut(s) 241, 575, 643, 808, 1202
BccI CCATC 4 cut(s) 62, 134, 368, 1122
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 33, 408, 425
BfaI CTAG 3 cut(s) 158, 761, 920
BfmI CTRYAG 2 cut(s) 336, 1216
BfuAI ACCTGC 1 cut(s) 845
BisI GCNGC 7 cut(s) 255, 564, 657, 810, 813, 822, 1216
BlpI GCTNAGC 1 cut(s) 107
BlsI GCNGC 7 cut(s) 256, 565, 658, 811, 814, 823, 1217
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 186
BmgT120I GGNCC 2 cut(s) 186, 362
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 96
BmrI ACTGGG 1 cut(s) 782
BmsI GCATC 4 cut(s) 139, 517, 558, 595
BmuI ACTGGG 1 cut(s) 782
BpiI GAAGAC 1 cut(s) 990
BpmI CTGGAG 3 cut(s) 478, 636, 1004
Bpu1102I GCTNAGC 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 873
BpuMI CCSGG 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 94, 709
BsaWI WCCGGW 1 cut(s) 1069
BsaXI ACNNNNNCTCC 2 cut(s) 1165, 1195
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse118I RCCGGY 1 cut(s) 1069
Bse1I ACTGG 7 cut(s) 199, 482, 619, 777, 784, 987, 1099
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 94, 709
BseGI GGATG 1 cut(s) 145
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 3 cut(s) 121, 735, 939
BseNI ACTGG 7 cut(s) 199, 482, 619, 777, 784, 987, 1099
BseRI GAGGAG 3 cut(s) 812, 1067, 1186
BseXI GCAGC 5 cut(s) 241, 575, 643, 808, 1202
BseYI CCCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 3 cut(s) 364, 739, 1208
BshTI ACCGGT 1 cut(s) 1069
BsiSI CCGG 3 cut(s) 96, 668, 1070
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 38
BsmAI GTCTC 3 cut(s) 33, 408, 425
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 3 cut(s) 364, 739, 1208
Bsp143I GATC 2 cut(s) 14, 1190
Bsp1720I GCTNAGC 1 cut(s) 107
BspACI CCGC 4 cut(s) 810, 813, 852, 1151
BspANI GGCC 3 cut(s) 364, 739, 1208
BspCNI CTCAG 3 cut(s) 120, 736, 940
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 1220
BspMI ACCTGC 1 cut(s) 845
BspQI GCTCTTC 1 cut(s) 945
BsrDI GCAATG 1 cut(s) 1008
BsrFI RCCGGY 1 cut(s) 1069
BsrI ACTGG 7 cut(s) 199, 482, 619, 777, 784, 987, 1099
BssAI RCCGGY 1 cut(s) 1069
BssECI CCNNGG 2 cut(s) 94, 709
BssMI GATC 2 cut(s) 14, 1190
BssT1I CCWWGG 1 cut(s) 709
Bst4CI ACNGT 6 cut(s) 55, 242, 290, 647, 928, 994
Bst6I CTCTTC 2 cut(s) 945, 1125
BstAPI GCANNNNNTGC 2 cut(s) 656, 821
BstC8I GCNNGC 2 cut(s) 1110, 1220
BstDEI CTNAG 7 cut(s) 59, 107, 115, 523, 744, 825, 948
BstF5I GGATG 1 cut(s) 145
BstHHI GCGC 2 cut(s) 586, 1224
BstKTI GATC 2 cut(s) 17, 1193
BstMAI GTCTC 3 cut(s) 33, 408, 425
BstMBI GATC 2 cut(s) 14, 1190
BstMWI GCNNNNNNNGC 7 cut(s) 133, 158, 656, 677, 818, 821, 1221
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 2 cut(s) 336, 1216
BstV1I GCAGC 5 cut(s) 241, 575, 643, 808, 1202
BstV2I GAAGAC 1 cut(s) 990
BsuRI GGCC 3 cut(s) 364, 739, 1208
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 8 cut(s) 51, 192, 207, 286, 770, 980, 999, 1008
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 1110, 1220
CaiI CAGNNNCTG 2 cut(s) 136, 656
CfoI GCGC 2 cut(s) 586, 1224
Cfr10I RCCGGY 1 cut(s) 1069
Cfr13I GGNCC 2 cut(s) 186, 362
Csp6I GTAC 1 cut(s) 777
CspAI ACCGGT 1 cut(s) 1069
CviAII CATG 4 cut(s) 864, 904, 1019, 1199
CviQI GTAC 1 cut(s) 777
DdeI CTNAG 7 cut(s) 59, 107, 115, 523, 744, 825, 948
DpnI GATC 2 cut(s) 16, 1192
DpnII GATC 2 cut(s) 14, 1190
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
Eam1104I CTCTTC 2 cut(s) 945, 1125
EarI CTCTTC 2 cut(s) 945, 1125
Eco130I CCWWGG 1 cut(s) 709
Eco47I GGWCC 1 cut(s) 186
Eco57I CTGAAG 5 cut(s) 8, 113, 461, 543, 984
EcoRI GAATTC 2 cut(s) 629, 1155
EcoT14I CCWWGG 1 cut(s) 709
EcoT22I ATGCAT 1 cut(s) 551
ErhI CCWWGG 1 cut(s) 709
FaeI CATG 4 cut(s) 867, 907, 1022, 1202
FaqI GGGAC 1 cut(s) 199
FatI CATG 4 cut(s) 863, 903, 1018, 1198
FauI CCCGC 1 cut(s) 1144
FauNDI CATATG 1 cut(s) 545
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 7 cut(s) 255, 564, 657, 810, 813, 822, 1216
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 7 cut(s) 255, 564, 657, 810, 813, 822, 1216
FspBI CTAG 3 cut(s) 158, 761, 920
GlaI GCGC 2 cut(s) 585, 1223
GluI GCNGC 7 cut(s) 255, 564, 657, 810, 813, 822, 1216
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 3 cut(s) 478, 636, 1004
HaeIII GGCC 3 cut(s) 364, 739, 1208
HapII CCGG 3 cut(s) 96, 668, 1070
HhaI GCGC 2 cut(s) 586, 1224
Hin1II CATG 4 cut(s) 867, 907, 1022, 1202
Hin6I GCGC 2 cut(s) 584, 1222
HinP1I GCGC 2 cut(s) 584, 1222
HincII GTYRAC 2 cut(s) 877, 910
HindII GTYRAC 2 cut(s) 877, 910
HinfI GANTC 4 cut(s) 119, 756, 967, 977
HpaII CCGG 3 cut(s) 96, 668, 1070
HphI GGTGA 4 cut(s) 155, 341, 1093, 1185
Hpy166II GTNNAC 4 cut(s) 877, 910, 1006, 1122
Hpy188I TCNGA 7 cut(s) 124, 132, 541, 554, 639, 949, 966
Hpy188III TCNNGA 4 cut(s) 281, 609, 971, 1181
Hpy8I GTNNAC 4 cut(s) 877, 910, 1006, 1122
HpyAV CCTTC 4 cut(s) 32, 971, 1198, 1205
HpyCH4III ACNGT 6 cut(s) 55, 242, 290, 647, 928, 994
HpyCH4IV ACGT 2 cut(s) 444, 879
HpyCH4V TGCA 5 cut(s) 152, 182, 549, 650, 1218
HpyF10VI GCNNNNNNNGC 7 cut(s) 133, 158, 656, 677, 818, 821, 1221
HpyF3I CTNAG 7 cut(s) 59, 107, 115, 523, 744, 825, 948
HpySE526I ACGT 2 cut(s) 444, 879
Hsp92II CATG 4 cut(s) 867, 907, 1022, 1202
HspAI GCGC 2 cut(s) 584, 1222
Kzo9I GATC 2 cut(s) 14, 1190
LguI GCTCTTC 1 cut(s) 945
Lsp1109I GCAGC 5 cut(s) 241, 575, 643, 808, 1202
LweI GCATC 4 cut(s) 139, 517, 558, 595
MaeI CTAG 3 cut(s) 158, 761, 920
MaeII ACGT 2 cut(s) 444, 879
MaeIII GTNAC 5 cut(s) 49, 205, 691, 899, 1226
MalI GATC 2 cut(s) 16, 1192
MboI GATC 2 cut(s) 14, 1190
MboII GAAGA 7 cut(s) 158, 292, 481, 523, 932, 990, 1142
MluCI AATT 6 cut(s) 177, 324, 604, 629, 955, 1155
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 2 cut(s) 564, 1027
Mph1103I ATGCAT 1 cut(s) 551
MseI TTAA 3 cut(s) 267, 507, 1061
MslI CAYNNNNRTG 1 cut(s) 212
MspI CCGG 3 cut(s) 96, 668, 1070
MspR9I CCNGG 1 cut(s) 96
MwoI GCNNNNNNNGC 7 cut(s) 133, 158, 656, 677, 818, 821, 1221
NciI CCSGG 1 cut(s) 96
NdeI CATATG 1 cut(s) 545
NdeII GATC 2 cut(s) 14, 1190
NlaIII CATG 4 cut(s) 867, 907, 1022, 1202
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 3 cut(s) 49, 205, 899
NsiI ATGCAT 1 cut(s) 551
PaqCI CACCTGC 1 cut(s) 845
PciSI GCTCTTC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PfeI GAWTC 3 cut(s) 756, 967, 977
PflFI GACNNNGTC 1 cut(s) 905
PinAI ACCGGT 1 cut(s) 1069
PkrI GCNGC 7 cut(s) 256, 565, 658, 811, 814, 823, 1217
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp1406I AACGTT 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 186, 362
PstI CTGCAG 1 cut(s) 1220
PstNI CAGNNNCTG 2 cut(s) 136, 656
PsyI GACNNNGTC 1 cut(s) 905
RsaI GTAC 1 cut(s) 778
RsaNI GTAC 1 cut(s) 777
RseI CAYNNNNRTG 1 cut(s) 212
SalI GTCGAC 1 cut(s) 908
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 3 cut(s) 267, 507, 1061
SatI GCNGC 7 cut(s) 255, 564, 657, 810, 813, 822, 1216
Sau3AI GATC 2 cut(s) 14, 1190
Sau96I GGNCC 2 cut(s) 186, 362
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 96
SfaNI GCATC 4 cut(s) 139, 517, 558, 595
SfcI CTRYAG 2 cut(s) 336, 1216
SinI GGWCC 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 888
SmoI CTYRAG 1 cut(s) 888
Sse9I AATT 6 cut(s) 177, 324, 604, 629, 955, 1155
SsiI CCGC 4 cut(s) 810, 813, 852, 1151
SspI AATATT 1 cut(s) 249
SspMI CTAG 3 cut(s) 158, 761, 920
StyD4I CCNGG 1 cut(s) 94
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 6 cut(s) 55, 242, 290, 647, 928, 994
TaiI ACGT 2 cut(s) 447, 882
TaqI TCGA 1 cut(s) 909
TasI AATT 6 cut(s) 177, 324, 604, 629, 955, 1155
TauI GCSGC 2 cut(s) 812, 815
TfiI GAWTC 3 cut(s) 756, 967, 977
Tru1I TTAA 3 cut(s) 267, 507, 1061
Tru9I TTAA 3 cut(s) 267, 507, 1061
TscAI CASTG 8 cut(s) 58, 199, 214, 293, 777, 987, 999, 1008
TseFI GTSAC 3 cut(s) 49, 205, 899
TseI GCWGC 5 cut(s) 254, 563, 656, 821, 1215
Tsp45I GTSAC 3 cut(s) 49, 205, 899
TspDTI ATGAA 4 cut(s) 246, 852, 932, 1143
TspRI CASTG 8 cut(s) 58, 199, 214, 293, 777, 987, 999, 1008
Tth111I GACNNNGTC 1 cut(s) 905
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 3 cut(s) 604, 629, 1155
XmiI GTMKAC 1 cut(s) 909
XspI CTAG 3 cut(s) 158, 761, 920
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.