pycom08g08640

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000265
Physical Location & Seq
Reverse (-)
6921768 .. 6923322
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08640.1

Sequence Viewer

Length: 1134 bp
ATGCTAACAACTCCAGATGAGAATAACCGTCCTGTATTTGCTGCCAAAGATATAAAGGACTTCTATCTTACTGAGAGCCCTAAAATCTTCCCCCAAAATACTTGTTGGTTGTTTCCTTATATTACGAAGATTATCAAAAGTCTAACAGGACCAAAGTACAATGGGAATTATCTGCATAGCTTGGTTAGGCAAAAGCTTGGTGAAAAAAAGCTCCACCAGACATTGACTAATGTTGTCATTCCTACATTTGACATAAAGAATCTCCAGCCAACAATCTTTTCTAGCTTTGAGGTTCACCTTCTTCTCTTCACCTCTTCGGTTTGTTCTCAAGGTTTAATTTTACCCTTACATGCCTTGCTGGACATATATCTGTCGCTGAGTTATAATATCCACTTTTCTTGGGTGATCGATCAGGTGAAACATAAGCCATCTTTTGATGCCTTAGTCTCAGACATATGCATTGCAACCTCAGCAGCACCAACTTATCTTCCAGCTCATTATTTTGAAACCAAGGACCACAATGGAAATGTTAGAGAATTCAACCTAATCGATGGTGGAGTGGCTGCAAATAATCCGACTTTAGTTGCTATTGGTGAAGTGACAAAGCAAATAATCAAGGGGAGCCCGGACTTCTTTCCTATCAAACCTATGGACTATGGACGATTTCTAGTCATATCATTAGGAACTGGCTCACCAAAAGCTGAATTGAAATATAACGCACATGATGCATCTAAATGGGGCTTATTAAATTGGTTAACCAGCGGAGGCTCGACACCGATCATCAATGTTTTCAGTCACGCAACTGCTGATTTGGTTGATCTCAACCTTTCTGTGGTTTTCCAAGCCCTTCACTCGGAGAAAAATTACCTTCGAATTCAGGATGACAAGCTGCATGGGGACATATCTTCTGTGGATATAGCAACAGAGAAGAATTTGGACAATCTTGTGAAAGTTGGTGAAGGCCTATTGAAGCAACCTGTTTCTAGGGTTAATTTAGAGACAGGAAAATTCGAAGCTTGCAATCATGAGACTAATGAAGAAGCCCTTATAAGGTTTGCAAAACTACTCTCTGAAGAAAAGTGGCTTCGTCTTGCAAAGTCGCCACAGGGTCAATGTATTGCAAAATCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

378

Amino Acids

42.08

Weight (kDa)

6.89

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 48 - 197 6.8e-10 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 384, 1049
AciI CCGC 1 cut(s) 762
AcsI RAATTY 4 cut(s) 536, 873, 931, 1007
AcuI CTGAAG 1 cut(s) 1092
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 832, 853, 1050
AgsI TTSAA 4 cut(s) 506, 541, 709, 970
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 8 cut(s) 180, 196, 211, 285, 494, 701, 889, 1016
AluI AGCT 8 cut(s) 180, 196, 211, 285, 494, 701, 889, 1016
Alw26I GTCTC 3 cut(s) 451, 992, 1022
AoxI GGCC 1 cut(s) 961
ApeKI GCWGC 4 cut(s) 41, 473, 563, 889
ApoI RAATTY 4 cut(s) 536, 873, 931, 1007
AspS9I GGNCC 2 cut(s) 149, 514
AsuC2I CCSGG 1 cut(s) 626
AsuHPI GGTGA 8 cut(s) 212, 287, 301, 415, 427, 605, 684, 968
AsuII TTCGAA 2 cut(s) 871, 1011
AvaII GGWCC 2 cut(s) 149, 514
BanII GRGCYC 2 cut(s) 80, 626
BbvCI CCTCAGC 1 cut(s) 469
BbvI GCAGC 4 cut(s) 28, 485, 550, 876
BccI CCATC 2 cut(s) 436, 545
BcnI CCSGG 1 cut(s) 626
BcoDI GTCTC 3 cut(s) 451, 992, 1022
BfaI CTAG 3 cut(s) 282, 668, 984
BisI GCNGC 4 cut(s) 42, 474, 564, 890
BlsI GCNGC 4 cut(s) 43, 475, 565, 891
Bme1390I CCNGG 1 cut(s) 626
Bme18I GGWCC 2 cut(s) 149, 514
BmgT120I GGNCC 2 cut(s) 149, 514
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 1 cut(s) 626
BmsI GCATC 3 cut(s) 427, 715, 737
BpmI CTGGAG 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 469
Bpu14I TTCGAA 2 cut(s) 871, 1011
BpuEI CTTGAG 1 cut(s) 312
BpuMI CCSGG 1 cut(s) 626
Bsa29I ATCGAT 2 cut(s) 408, 549
BsaJI CCNNGG 1 cut(s) 510
Bsc4I CCNNNNNNNGG 3 cut(s) 832, 853, 1050
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 1 cut(s) 459
BseCI ATCGAT 2 cut(s) 408, 549
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 1 cut(s) 886
BseLI CCNNNNNNNGG 3 cut(s) 832, 853, 1050
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 4 cut(s) 63, 368, 462, 483
BseNI ACTGG 1 cut(s) 691
BseXI GCAGC 4 cut(s) 28, 485, 550, 876
BshFI GGCC 1 cut(s) 963
BshVI ATCGAT 2 cut(s) 408, 549
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 3 cut(s) 832, 853, 1050
BsmAI GTCTC 3 cut(s) 451, 992, 1022
BsmFI GGGAC 1 cut(s) 911
BsnI GGCC 1 cut(s) 963
Bsp119I TTCGAA 2 cut(s) 871, 1011
Bsp1286I GDGCHC 2 cut(s) 80, 626
Bsp143I GATC 4 cut(s) 405, 409, 777, 817
BspACI CCGC 1 cut(s) 762
BspANI GGCC 1 cut(s) 963
BspCNI CTCAG 4 cut(s) 64, 369, 461, 482
BspDI ATCGAT 2 cut(s) 408, 549
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 1 cut(s) 623
BspT104I TTCGAA 2 cut(s) 871, 1011
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 4 cut(s) 405, 409, 777, 817
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 2 cut(s) 311, 319
BstAPI GCANNNNNTGC 1 cut(s) 725
BstBI TTCGAA 2 cut(s) 871, 1011
BstC8I GCNNGC 1 cut(s) 1018
BstDEI CTNAG 5 cut(s) 72, 377, 442, 448, 469
BstF5I GGATG 1 cut(s) 886
BstKTI GATC 4 cut(s) 408, 412, 780, 820
BstMAI GTCTC 3 cut(s) 451, 992, 1022
BstMBI GATC 4 cut(s) 405, 409, 777, 817
BstMWI GCNNNNNNNGC 2 cut(s) 470, 725
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 624
BstV1I GCAGC 4 cut(s) 28, 485, 550, 876
Bsu15I ATCGAT 2 cut(s) 408, 549
BsuRI GGCC 1 cut(s) 963
BsuTUI ATCGAT 2 cut(s) 408, 549
BtsCI GGATG 1 cut(s) 886
Cac8I GCNNGC 1 cut(s) 1018
CciI TCATGA 1 cut(s) 1024
Cfr13I GGNCC 2 cut(s) 149, 514
ClaI ATCGAT 2 cut(s) 408, 549
Csp6I GTAC 1 cut(s) 157
CspCI CAANNNNNGTGG 2 cut(s) 380, 415
CviAII CATG 4 cut(s) 350, 722, 893, 1025
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 5 cut(s) 72, 377, 442, 448, 469
DpnI GATC 4 cut(s) 407, 411, 779, 819
DpnII GATC 4 cut(s) 405, 409, 777, 817
Eam1104I CTCTTC 2 cut(s) 311, 319
EarI CTCTTC 2 cut(s) 311, 319
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 963
Eco24I GRGCYC 2 cut(s) 80, 626
Eco47I GGWCC 2 cut(s) 149, 514
Eco57I CTGAAG 1 cut(s) 1092
EcoRI GAATTC 2 cut(s) 536, 873
EcoT14I CCWWGG 1 cut(s) 510
EcoT22I ATGCAT 2 cut(s) 461, 730
EcoT38I GRGCYC 2 cut(s) 80, 626
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 353, 725, 896, 1028
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FaqI GGGAC 1 cut(s) 911
FatI CATG 4 cut(s) 349, 721, 892, 1024
FauNDI CATATG 1 cut(s) 455
Fnu4HI GCNGC 4 cut(s) 42, 474, 564, 890
FokI GGATG 1 cut(s) 893
FriOI GRGCYC 2 cut(s) 80, 626
Fsp4HI GCNGC 4 cut(s) 42, 474, 564, 890
FspBI CTAG 3 cut(s) 282, 668, 984
GluI GCNGC 4 cut(s) 42, 474, 564, 890
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 963
HapII CCGG 1 cut(s) 626
Hin1II CATG 4 cut(s) 353, 725, 896, 1028
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HindIII AAGCTT 2 cut(s) 194, 1014
HinfI GANTC 1 cut(s) 259
HpaI GTTAAC 1 cut(s) 756
HpaII CCGG 1 cut(s) 626
HphI GGTGA 8 cut(s) 212, 287, 301, 415, 427, 605, 684, 968
Hpy166II GTNNAC 2 cut(s) 295, 756
Hpy188I TCNGA 4 cut(s) 451, 576, 856, 1072
Hpy188III TCNNGA 3 cut(s) 14, 878, 1025
Hpy8I GTNNAC 2 cut(s) 295, 756
HpyAV CCTTC 4 cut(s) 308, 857, 878, 953
HpyCH4III ACNGT 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 725
HpyF3I CTNAG 5 cut(s) 72, 377, 442, 448, 469
Hsp92II CATG 4 cut(s) 353, 725, 896, 1028
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 4 cut(s) 405, 409, 777, 817
LmnI GCTCC 2 cut(s) 216, 621
Lsp1109I GCAGC 4 cut(s) 28, 485, 550, 876
LweI GCATC 3 cut(s) 427, 715, 737
MaeI CTAG 3 cut(s) 282, 668, 984
MaeIII GTNAC 2 cut(s) 598, 794
MalI GATC 4 cut(s) 407, 411, 779, 819
MboI GATC 4 cut(s) 405, 409, 777, 817
MhlI GDGCHC 2 cut(s) 80, 626
MmeI TCCRAC 1 cut(s) 599
MnlI CCTC 4 cut(s) 283, 322, 478, 758
Mph1103I ATGCAT 2 cut(s) 461, 730
MseI TTAA 4 cut(s) 335, 746, 755, 990
MslI CAYNNNNRTG 1 cut(s) 733
MspA1I CMGCKG 1 cut(s) 762
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 1 cut(s) 626
MwoI GCNNNNNNNGC 2 cut(s) 470, 725
NciI CCSGG 1 cut(s) 626
NdeI CATATG 1 cut(s) 455
NdeII GATC 4 cut(s) 405, 409, 777, 817
NlaIII CATG 4 cut(s) 353, 725, 896, 1028
NlaIV GGNNCC 1 cut(s) 623
NmuCI GTSAC 2 cut(s) 598, 794
NsiI ATGCAT 2 cut(s) 461, 730
NspI RCATGY 1 cut(s) 353
NspV TTCGAA 2 cut(s) 871, 1011
PagI TCATGA 1 cut(s) 1024
PceI AGGCCT 1 cut(s) 963
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 4 cut(s) 43, 475, 565, 891
PsiI TTATAA 2 cut(s) 384, 1049
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 2 cut(s) 149, 514
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 733
SaqAI TTAA 4 cut(s) 335, 746, 755, 990
SatI GCNGC 4 cut(s) 42, 474, 564, 890
Sau3AI GATC 4 cut(s) 405, 409, 777, 817
Sau96I GGNCC 2 cut(s) 149, 514
ScrFI CCNGG 1 cut(s) 626
SduI GDGCHC 2 cut(s) 80, 626
SfaNI GCATC 3 cut(s) 427, 715, 737
SfuI TTCGAA 2 cut(s) 871, 1011
SinI GGWCC 2 cut(s) 149, 514
SmiMI CAYNNNNRTG 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SseBI AGGCCT 1 cut(s) 963
SsiI CCGC 1 cut(s) 762
SspMI CTAG 3 cut(s) 282, 668, 984
StuI AGGCCT 1 cut(s) 963
StyD4I CCNGG 1 cut(s) 624
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 1 cut(s) 29
TaqI TCGA 5 cut(s) 408, 549, 770, 871, 1011
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 4 cut(s) 335, 746, 755, 990
Tru9I TTAA 4 cut(s) 335, 746, 755, 990
TseFI GTSAC 2 cut(s) 598, 794
TseI GCWGC 4 cut(s) 41, 473, 563, 889
Tsp45I GTSAC 2 cut(s) 598, 794
TspDTI ATGAA 1 cut(s) 1050
VpaK11BI GGWCC 2 cut(s) 149, 514
XapI RAATTY 4 cut(s) 536, 873, 931, 1007
XceI RCATGY 1 cut(s) 353
XspI CTAG 3 cut(s) 282, 668, 984
Zsp2I ATGCAT 2 cut(s) 461, 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.