Rh5DG283400

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000265
Physical Location & Seq
Reverse (-)
34848209 .. 34869134
20926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG283400.1

Sequence Viewer

Length: 540 bp
ATGGGTGGTGGTGAGACAGTGGTGTTGGTGCAAGGAGTGAGTTGGGCTGTACTAGTGGGCTGGTGTAATCCCATTGCTATCATGGATATATCATGGAGGGGTATCTCTCTTGGTCCAAGATGTCGAAAGAACTTCTTGACATCAATTCAAAGTTTGGTTGGAGCGGTGACCGGACCAAAATACGACGGGAATTACCTGCGCACATTGACGACCGGGCTGCTTGGCGACCTCACAATAAAAGAAACTCTAACCAATGTCATCATCCCAACGTTCGATATCAAACACCTTCAGCCTGTGATTTTCTCAACCATTGATGATGATAATTTGATCGGTGAGGAGGCATCAGTGGACATTGCAACAGAGAAGAATCTGAAGAGGCTTGTGGAGATCGGAAATGCGCTGTTGAAGAAGCGATTGTCAAGGGTAAATCTCGATACTGGGAGGTACGAGGAATCTGAGGGAGAAGGTACCTACGAAGAAGTACTGGTCGATTTTGCCAAACGGCTCGCGGAGGGAAGGAAGCTCAGGCAAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004620 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0044424 GO:0044464 GO:0047372 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

179

Amino Acids

19.76

Weight (kDa)

5.93

Isoelectric Point (pI)

23.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 204
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 1 cut(s) 509
AciI CCGC 2 cut(s) 164, 509
AclI AACGTT 1 cut(s) 269
AcuI CTGAAG 2 cut(s) 272, 392
AfaI GTAC 4 cut(s) 51, 446, 469, 483
AgsI TTSAA 2 cut(s) 149, 406
AhlI ACTAGT 1 cut(s) 52
AluBI AGCT 1 cut(s) 523
AluI AGCT 1 cut(s) 523
Alw26I GTCTC 1 cut(s) 8
ApeKI GCWGC 1 cut(s) 217
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 2 cut(s) 201, 400
AspS9I GGNCC 2 cut(s) 113, 173
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 3 cut(s) 23, 178, 344
AvaII GGWCC 2 cut(s) 113, 173
BanI GGYRCC 1 cut(s) 467
BbvI GCAGC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 518
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 1 cut(s) 53
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 1 cut(s) 218
BlsI GCNGC 1 cut(s) 219
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 2 cut(s) 113, 173
BmgT120I GGNCC 2 cut(s) 113, 173
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 1 cut(s) 214
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 1 cut(s) 350
BmuI ACTGGG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 214
BsaWI WCCGGW 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 442, 489
Bse3DI GCAATG 2 cut(s) 72, 351
BseGI GGATG 1 cut(s) 261
BseMI GCAATG 2 cut(s) 72, 351
BseMII CTCAG 2 cut(s) 447, 538
BseNI ACTGG 2 cut(s) 442, 489
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 213
BshNI GGYRCC 1 cut(s) 467
BsiEI CGRYCG 1 cut(s) 213
BsiSI CCGG 2 cut(s) 171, 213
BsmAI GTCTC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 327, 387
BspACI CCGC 2 cut(s) 164, 509
BspCNI CTCAG 2 cut(s) 448, 537
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 164
BsrDI GCAATG 2 cut(s) 72, 351
BsrI ACTGG 2 cut(s) 442, 489
BssMI GATC 2 cut(s) 327, 387
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 2 cut(s) 456, 524
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 509
BstHHI GCGC 2 cut(s) 201, 400
BstKTI GATC 2 cut(s) 330, 390
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 2 cut(s) 327, 387
BstMCI CGRYCG 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 212
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 1 cut(s) 204
BtsCI GGATG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 24, 351
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 507
CfoI GCGC 2 cut(s) 201, 400
Cfr13I GGNCC 2 cut(s) 113, 173
Csp6I GTAC 4 cut(s) 50, 445, 468, 482
CviAII CATG 2 cut(s) 82, 93
CviJI RGCY 7 cut(s) 47, 60, 217, 292, 379, 505, 523
CviKI_1 RGCY 7 cut(s) 47, 60, 217, 292, 379, 505, 523
CviQI GTAC 4 cut(s) 50, 445, 468, 482
DdeI CTNAG 2 cut(s) 456, 524
DpnI GATC 2 cut(s) 329, 389
DpnII GATC 2 cut(s) 327, 387
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 113, 173
Eco57I CTGAAG 2 cut(s) 272, 392
Eco91I GGTNACC 1 cut(s) 166
EcoO65I GGTNACC 1 cut(s) 166
EcoRV GATATC 1 cut(s) 277
FaeI CATG 2 cut(s) 85, 96
FaiI YATR 3 cut(s) 83, 89, 94
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FatI CATG 2 cut(s) 81, 92
Fnu4HI GCNGC 1 cut(s) 218
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 1 cut(s) 218
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 2 cut(s) 200, 399
GluI GCNGC 1 cut(s) 218
HapII CCGG 2 cut(s) 171, 213
HhaI GCGC 2 cut(s) 201, 400
Hin1II CATG 2 cut(s) 85, 96
Hin6I GCGC 2 cut(s) 199, 398
HinP1I GCGC 2 cut(s) 199, 398
HinfI GANTC 2 cut(s) 367, 452
HpaII CCGG 2 cut(s) 171, 213
HphI GGTGA 3 cut(s) 23, 178, 344
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 3 cut(s) 372, 392, 457
Hpy188III TCNNGA 2 cut(s) 136, 431
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 296, 458, 510
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 2 cut(s) 31, 356
HpyF3I CTNAG 2 cut(s) 456, 524
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 2 cut(s) 85, 96
HspAI GCGC 2 cut(s) 199, 398
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 2 cut(s) 327, 387
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 8 cut(s) 46, 184, 209, 226, 306, 423, 470, 511
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 1 cut(s) 350
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 329, 389
MbiI CCGCTC 1 cut(s) 164
MboI GATC 2 cut(s) 327, 387
MboII GAAGA 4 cut(s) 376, 385, 418, 488
MluCI AATT 4 cut(s) 144, 190, 322, 535
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 9 cut(s) 90, 239, 328, 331, 369, 435, 442, 451, 505
MspI CCGG 2 cut(s) 171, 213
MspR9I CCNGG 1 cut(s) 214
MvnI CGCG 1 cut(s) 509
NciI CCSGG 1 cut(s) 214
NdeII GATC 2 cut(s) 327, 387
NlaIII CATG 2 cut(s) 85, 96
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 166
NsbI TGCGCA 1 cut(s) 200
PfeI GAWTC 2 cut(s) 367, 452
PkrI GCNGC 1 cut(s) 219
Psp1406I AACGTT 1 cut(s) 269
PspEI GGTNACC 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 2 cut(s) 113, 173
RsaI GTAC 4 cut(s) 51, 446, 469, 483
RsaNI GTAC 4 cut(s) 50, 445, 468, 482
SatI GCNGC 1 cut(s) 218
Sau3AI GATC 2 cut(s) 327, 387
Sau96I GGNCC 2 cut(s) 113, 173
ScaI AGTACT 1 cut(s) 483
ScrFI CCNGG 1 cut(s) 214
SetI ASST 8 cut(s) 198, 231, 272, 288, 446, 469, 473, 525
SfaNI GCATC 1 cut(s) 350
SinI GGWCC 2 cut(s) 113, 173
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 4 cut(s) 144, 190, 322, 535
SsiI CCGC 2 cut(s) 164, 509
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 212
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 272
TaqI TCGA 4 cut(s) 124, 273, 432, 489
TasI AATT 4 cut(s) 144, 190, 322, 535
TatI WGTACW 2 cut(s) 49, 481
TfiI GAWTC 2 cut(s) 367, 452
TscAI CASTG 2 cut(s) 24, 351
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 166
TspRI CASTG 2 cut(s) 24, 351
VpaK11BI GGWCC 2 cut(s) 113, 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XspI CTAG 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.