Rw6G040920

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000265
Physical Location & Seq
Forward (+)
64662298 .. 64664183
1886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040920.1

Sequence Viewer

Length: 1224 bp
ATGGAAAGAACTGGTAGTATCACTTTACAGCCCCCAACCTTTGGAAACCTAATCACTGTTCTGAGCATTGACGGCGGTGGAATAAGAGGGCTTATCCCAGGAACCATCCTTGCTTTCCTTGAGTCTGAACTTCAGAAGCTGGACGGTGAAGATGCAAGACTCGCCGACTATTTCGATGTGATTTCAGGGACAAGCACAGGTGGTCTCGTAACTGCCATGCTCACAGCCCCAAATGAGAATGACCGTCCACTATTTGCTGCCAAAGATATCAAGGACTTCTACTTGACCCACTCCCCCAAAATCTTCCCCCAGAAGACTGGTTGGCTGTTTCCTCGTGCTAGAAAGATCATCAGAGCTCTAGCAGGACCGAAATACGATGGGAAGTATCTACATGGATTGGTTAGGGAAAAGCTTGGTGACAAAAAACTGAACCAAACATTGACTAATGTTGTCATTCCAGCATTTGACATAAAGAATCTGCAGCCAGCTATATTTTCCAGCTTCAAGGTGAAAAATAAGCCTTCCTTTGATGCCCTACTTTCGGACATATGTATTGCAACCTCAGCGGCGCCAACTTACCTCCCAGCTCATTATTTTAAAACCAACAGCCTCGAAGGAAAAGTTAGAGAATTTCACCTGATAGATGGTGGATTGGCAGCAAATAATCCGACTTTGATTGCAATTGGTGAAGTGACAAAGGAATTAATTAAGGGGAGTTCAGACTTCTTTCCTATAAAACCAATGGACTATGGAAGATTTCTGGTGATATCCTTAGGAACTGGCTCATCGAAAGCTGAACTGAAATACAATGCTCATGTTGCCTCCAAGTGGGGTGTGTTGAATTGGTTAACGAGCGGTGGTTCCACCCCATTAATCAATGCTTTCAGTCAAGCAAGTGCCGATATGGTCGATTTGCATCTTTCTGTGGTTTTCAAAGCCCTTCACTCGGAAAAAAACTATCTTCGAATTCAGGATGACACGCTAAGCGGCGAAGTATCTTCCGTAGATATAGCAACACAGAAGAATCTGGACCATCTTCTCAGAGTTGGTGAAGGACTATTGAACCAACCAGTTTCCAGGGTTAATTTAGAGACAGGCAAATTCGAAGCTTGTAATCATGAGACTAATAAAGAGGCTCTTACAAGGTTTGCAAAATTGCTGTCCGAACAGAAGTGGCTTCGCCTTGCAAGGTCTCCCCATGGACATATTGCAAAGTCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

407

Amino Acids

44.6

Weight (kDa)

9.04

Isoelectric Point (pI)

26.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 228 8.9e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AccB7I CCANNNNNTGG 1 cut(s) 41
AccBSI CCGCTC 1 cut(s) 855
AciI CCGC 4 cut(s) 75, 566, 855, 987
AcsI RAATTY 3 cut(s) 629, 966, 1100
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 1 cut(s) 569
AfiI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
AgsI TTSAA 4 cut(s) 505, 841, 934, 1063
AjnI CCWGG 2 cut(s) 97, 1076
AluBI AGCT 8 cut(s) 139, 356, 412, 488, 501, 587, 794, 1109
AluI AGCT 8 cut(s) 139, 356, 412, 488, 501, 587, 794, 1109
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 4 cut(s) 209, 1085, 1115, 1197
AlwNI CAGNNNCTG 1 cut(s) 139
ApeKI GCWGC 3 cut(s) 257, 481, 656
ApoI RAATTY 3 cut(s) 629, 966, 1100
ArsI GACNNNNNNTTYG 2 cut(s) 1145, 1177
AseI ATTAAT 2 cut(s) 704, 872
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 2 cut(s) 365, 1030
AsuHPI GGTGA 7 cut(s) 158, 428, 520, 626, 698, 775, 1061
AsuII TTCGAA 2 cut(s) 964, 1104
AvaII GGWCC 2 cut(s) 365, 1030
AxyI CCTNAGG 1 cut(s) 772
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 358
BauI CACGAG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 3 cut(s) 244, 493, 668
BccI CCATC 4 cut(s) 113, 371, 638, 1041
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 2 cut(s) 99, 1078
BcoDI GTCTC 4 cut(s) 209, 1085, 1115, 1197
BfaI CTAG 2 cut(s) 339, 359
BfmI CTRYAG 1 cut(s) 479
BfoI RGCGCY 1 cut(s) 572
BisI GCNGC 5 cut(s) 258, 482, 567, 657, 988
BlpI GCTNAGC 1 cut(s) 983
BlsI GCNGC 5 cut(s) 259, 483, 568, 658, 989
Bme1390I CCNGG 2 cut(s) 99, 1078
Bme18I GGWCC 2 cut(s) 365, 1030
BmgT120I GGNCC 2 cut(s) 365, 1030
BmiI GGNNCC 3 cut(s) 103, 570, 862
BmrFI CCNGG 2 cut(s) 99, 1078
BmsI GCATC 3 cut(s) 142, 520, 925
BpiI GAAGAC 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 562
Bpu1102I GCTNAGC 1 cut(s) 983
Bpu14I TTCGAA 2 cut(s) 964, 1104
BpuEI CTTGAG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 915
BsaHI GRCGYC 1 cut(s) 569
BsaI GGTCTC 2 cut(s) 209, 1197
BsaJI CCNNGG 3 cut(s) 97, 1077, 1198
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
Bse1I ACTGG 4 cut(s) 16, 322, 784, 1070
Bse21I CCTNAGG 1 cut(s) 772
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 99, 1078
BseDI CCNNGG 3 cut(s) 97, 1077, 1198
BseGI GGATG 2 cut(s) 105, 979
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
BseMII CTCAG 3 cut(s) 53, 576, 1054
BseNI ACTGG 4 cut(s) 16, 322, 784, 1070
BseXI GCAGC 3 cut(s) 244, 493, 668
BseYI CCCAGC 1 cut(s) 583
BshNI GGYRCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 358
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
BsmAI GTCTC 4 cut(s) 209, 1085, 1115, 1197
BsmFI GGGAC 1 cut(s) 202
Bso31I GGTCTC 2 cut(s) 209, 1197
Bsp119I TTCGAA 2 cut(s) 964, 1104
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 1 cut(s) 345
Bsp1720I GCTNAGC 1 cut(s) 983
Bsp19I CCATGG 1 cut(s) 1198
BspACI CCGC 4 cut(s) 75, 566, 855, 987
BspCNI CTCAG 3 cut(s) 54, 575, 1053
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 3 cut(s) 103, 570, 862
BspMAI CTGCAG 1 cut(s) 483
BspT104I TTCGAA 2 cut(s) 964, 1104
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 2 cut(s) 209, 1197
BsrBI CCGCTC 1 cut(s) 855
BsrI ACTGG 4 cut(s) 16, 322, 784, 1070
BssECI CCNNGG 3 cut(s) 97, 1077, 1198
BssMI GATC 1 cut(s) 345
BssNI GRCGYC 1 cut(s) 569
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 1198
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 99, 1078
Bst4CI ACNGT 3 cut(s) 58, 146, 245
BstACI GRCGYC 1 cut(s) 569
BstBI TTCGAA 2 cut(s) 964, 1104
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 5 cut(s) 62, 562, 772, 983, 1040
BstDSI CCRYGG 1 cut(s) 1198
BstF5I GGATG 2 cut(s) 105, 979
BstH2I RGCGCY 1 cut(s) 572
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 1 cut(s) 348
BstMAI GTCTC 4 cut(s) 209, 1085, 1115, 1197
BstMBI GATC 1 cut(s) 345
BstMWI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
BstNI CCWGG 2 cut(s) 99, 1078
BstSCI CCNGG 2 cut(s) 97, 1076
BstSFI CTRYAG 1 cut(s) 479
BstV1I GCAGC 3 cut(s) 244, 493, 668
BstV2I GAAGAC 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 317
Bsu36I CCTNAGG 1 cut(s) 772
BtgI CCRYGG 1 cut(s) 1198
BtsCI GGATG 2 cut(s) 105, 979
BtsIMutI CAGTG 2 cut(s) 54, 1219
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 1117
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 2 cut(s) 365, 1030
CviAII CATG 5 cut(s) 217, 392, 815, 1118, 1199
DdeI CTNAG 5 cut(s) 62, 562, 772, 983, 1040
DinI GGCGCC 1 cut(s) 570
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
DraI TTTAAA 1 cut(s) 598
Ecl136II GAGCTC 1 cut(s) 356
Eco130I CCWWGG 1 cut(s) 1198
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 2 cut(s) 209, 1197
Eco32I GATATC 2 cut(s) 268, 768
Eco47I GGWCC 2 cut(s) 365, 1030
Eco53kI GAGCTC 1 cut(s) 356
Eco57I CTGAAG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 772
EcoICRI GAGCTC 1 cut(s) 356
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 2 cut(s) 97, 1076
EcoRV GATATC 2 cut(s) 268, 768
EcoT14I CCWWGG 1 cut(s) 1198
EcoT38I GRGCYC 1 cut(s) 358
EgeI GGCGCC 1 cut(s) 570
EheI GGCGCC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 1198
FaeI CATG 5 cut(s) 220, 395, 818, 1121, 1202
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 1122, 1154
FaqI GGGAC 1 cut(s) 202
FatI CATG 5 cut(s) 216, 391, 814, 1117, 1198
FauNDI CATATG 1 cut(s) 548
Fnu4HI GCNGC 5 cut(s) 258, 482, 567, 657, 988
FokI GGATG 2 cut(s) 92, 986
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 5 cut(s) 258, 482, 567, 657, 988
FspBI CTAG 2 cut(s) 339, 359
GlaI GCGC 1 cut(s) 570
GluI GCNGC 5 cut(s) 258, 482, 567, 657, 988
GsaI CCCAGC 1 cut(s) 587
HaeII RGCGCY 1 cut(s) 572
HhaI GCGC 1 cut(s) 571
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 5 cut(s) 220, 395, 818, 1121, 1202
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HindIII AAGCTT 2 cut(s) 410, 1107
HinfI GANTC 4 cut(s) 122, 159, 475, 1024
HpaI GTTAAC 1 cut(s) 849
HphI GGTGA 7 cut(s) 158, 428, 520, 626, 698, 775, 1061
Hpy166II GTNNAC 2 cut(s) 248, 849
Hpy188III TCNNGA 3 cut(s) 971, 1028, 1118
Hpy8I GTNNAC 2 cut(s) 248, 849
HpyAV CCTTC 4 cut(s) 531, 608, 950, 1046
HpyCH4III ACNGT 3 cut(s) 58, 146, 245
HpyCH4V TGCA 8 cut(s) 155, 481, 557, 680, 916, 1151, 1187, 1211
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
HpyF3I CTNAG 5 cut(s) 62, 562, 772, 983, 1040
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 5 cut(s) 220, 395, 818, 1121, 1202
HspAI GCGC 1 cut(s) 569
KasI GGCGCC 1 cut(s) 568
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 1 cut(s) 345
Lsp1109I GCAGC 3 cut(s) 244, 493, 668
LweI GCATC 3 cut(s) 142, 520, 925
MaeI CTAG 2 cut(s) 339, 359
MaeIII GTNAC 3 cut(s) 208, 416, 691
MalI GATC 1 cut(s) 347
MbiI CCGCTC 1 cut(s) 855
MboI GATC 1 cut(s) 345
MboII GAAGA 8 cut(s) 161, 295, 325, 765, 953, 990, 1028, 1033
MfeI CAATTG 1 cut(s) 681
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 9 cut(s) 629, 681, 701, 705, 841, 966, 1084, 1100, 1154
Mly113I GGCGCC 1 cut(s) 569
MlyI GAGTC 2 cut(s) 131, 153
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 7 cut(s) 80, 342, 571, 590, 620, 832, 1126
MseI TTAA 6 cut(s) 597, 704, 708, 848, 872, 1083
MspA1I CMGCKG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 99, 1078
MunI CAATTG 1 cut(s) 681
MvaI CCWGG 2 cut(s) 99, 1078
MwoI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
NarI GGCGCC 1 cut(s) 569
NcoI CCATGG 1 cut(s) 1198
NdeI CATATG 1 cut(s) 548
NdeII GATC 1 cut(s) 345
NlaIII CATG 5 cut(s) 220, 395, 818, 1121, 1202
NlaIV GGNNCC 3 cut(s) 103, 570, 862
NmuCI GTSAC 2 cut(s) 416, 691
NspV TTCGAA 2 cut(s) 964, 1104
PacI TTAATTAA 1 cut(s) 708
PagI TCATGA 1 cut(s) 1117
PfeI GAWTC 2 cut(s) 475, 1024
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 5 cut(s) 259, 483, 568, 658, 989
PleI GAGTC 2 cut(s) 130, 153
PluTI GGCGCC 1 cut(s) 572
PpsI GAGTC 2 cut(s) 130, 153
PshBI ATTAAT 2 cut(s) 704, 872
Psp124BI GAGCTC 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 97, 1076
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 2 cut(s) 97, 1076
PspN4I GGNNCC 3 cut(s) 103, 570, 862
PspPI GGNCC 2 cut(s) 365, 1030
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 1 cut(s) 139
SacI GAGCTC 1 cut(s) 358
SaqAI TTAA 6 cut(s) 597, 704, 708, 848, 872, 1083
SatI GCNGC 5 cut(s) 258, 482, 567, 657, 988
Sau3AI GATC 1 cut(s) 345
Sau96I GGNCC 2 cut(s) 365, 1030
SchI GAGTC 2 cut(s) 131, 153
ScrFI CCNGG 2 cut(s) 99, 1078
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 3 cut(s) 142, 520, 925
SfcI CTRYAG 1 cut(s) 479
SfoI GGCGCC 1 cut(s) 570
SfuI TTCGAA 2 cut(s) 964, 1104
SinI GGWCC 2 cut(s) 365, 1030
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 9 cut(s) 629, 681, 701, 705, 841, 966, 1084, 1100, 1154
SsiI CCGC 4 cut(s) 75, 566, 855, 987
SspDI GGCGCC 1 cut(s) 568
SspMI CTAG 2 cut(s) 339, 359
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 97, 1076
StyI CCWWGG 1 cut(s) 1198
TaaI ACNGT 3 cut(s) 58, 146, 245
TaqI TCGA 6 cut(s) 174, 612, 788, 909, 964, 1104
TaqII GACCGA 1 cut(s) 382
TasI AATT 9 cut(s) 629, 681, 701, 705, 841, 966, 1084, 1100, 1154
TauI GCSGC 2 cut(s) 569, 990
TfiI GAWTC 2 cut(s) 475, 1024
Tru1I TTAA 6 cut(s) 597, 704, 708, 848, 872, 1083
Tru9I TTAA 6 cut(s) 597, 704, 708, 848, 872, 1083
TscAI CASTG 1 cut(s) 61
TseFI GTSAC 2 cut(s) 416, 691
TseI GCWGC 3 cut(s) 257, 481, 656
Tsp45I GTSAC 2 cut(s) 416, 691
TspGWI ACGGA 1 cut(s) 991
TspRI CASTG 1 cut(s) 61
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 365, 1030
VspI ATTAAT 2 cut(s) 704, 872
XapI RAATTY 3 cut(s) 629, 966, 1100
XspI CTAG 2 cut(s) 339, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.