Rh6DG470600

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000265
Physical Location & Seq
Reverse (-)
63748406 .. 63751106
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG470600.1

Sequence Viewer

Length: 1254 bp
ATGGCCATGGAGAGAGCAAGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATAAGAGGGATTATCCCCGGAACTATGCTCAGCTTCTTAGAGTCCGAGCTTCAGAAGCTGGATGGTGAAGATGCAAGACTAGCAGATTATTTTGATGTAATTGCAGGGACCAGCACAGGTGGTCTTGTCACTGCTATGCTTACTGCCCCAGATGAAAATAACCGTCCAATATTTGCTGCTAAAGATATTAACGATTTTTATCTTGAACACTGTCCTAAAATCTTCCCCCAAGACAGCACCTCAATAATTGGTGAAGCTGTAGAAAAGTTGAAAGTTGTGAAAGGGCCAAAATATGATGGGGAGTATTTACATAAGATAGTCAGGGAAAAGTTGGGAGACATACGATTGAACGAGACATTGACTAACGTTGTAATCCCAACTTTTGATATAAAGAGGCTTCAGCCAGTCATCTTCTCCAGCTATGAGGTTAAGAAGAATGATAGCTTAGATGCTTTACTCTCCGACATATGCATTGGAACTTCAGCAGCCCCAACTTATCTCCCAGCGCATCATTTTGAAACCCAAAATTCCAGAACTGGAGAAGCAAGAGAATTCAATCTGATTGACGGTGCAGTCGCTGCTAATAATCCGGCTTTGGTCGCTATAAACGAAGTAACAAAAGAAATCCACCAAGGAAACACCGACTTCTTTCCTATGAGGCCAACTGAGTATGGGCGATTCCTCGTTATATCACTGGGTACTGGTTCAGGGAAATGCGAGGAGAAGTATGCCGCCGCAAATGCTGCTAAGTGGGGCGTTTTGGGGTGGCTAACCGCAGGTGGTTCATGTCCATTGGTTGACGTATTTACTCAAGCAAGTAGTGACATGGTCGACTTCCACCTAGCCACCGTTTTCCAAGCTCTTTATTCTGAGAAAAATTACCTTCGGATTCAAGACGATTCACTGGAGGGAACAGTGTCTTCAGTGGACATTGCTACGCATGAGAACTTGAACAACCTTGTAAAAGTTGGAGAGGAGTTGTTAAAGAAACCGGTTTCAAGGGTGAATATGGAAACTGGTATATATGAGCCTGCCCATCAGTCCACAAATGAAGAGGCTCTCGTAAGGGTAGCGGGAATTCTGTCTAAAGAGAGGAGAGTTCGTGAAATGAGATCACCACATGGGAAGGCTGCAGGCACAGTTACTTCTAATACTACTAGCTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

417

Amino Acids

45.54

Weight (kDa)

5.21

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 230 1.1e-24 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 851
AccI GTMKAC 1 cut(s) 915
AciI CCGC 4 cut(s) 816, 819, 858, 1157
AclI AACGTT 1 cut(s) 450
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 610, 635, 1161
AcuI CTGAAG 5 cut(s) 14, 119, 467, 549, 990
AfaI GTAC 1 cut(s) 784
AfiI CCNNNNNNNGG 1 cut(s) 44
AgeI ACCGGT 1 cut(s) 1075
AgsI TTSAA 8 cut(s) 290, 355, 433, 602, 640, 977, 1036, 1083
AluBI AGCT 8 cut(s) 117, 133, 142, 341, 504, 528, 944, 1245
AluI AGCT 8 cut(s) 117, 133, 142, 341, 504, 528, 944, 1245
Alw26I GTCTC 3 cut(s) 39, 414, 431
AlwNI CAGNNNCTG 2 cut(s) 142, 662
AoxI GGCC 3 cut(s) 3, 368, 743
ApeKI GCWGC 5 cut(s) 260, 569, 662, 827, 1214
ApoI RAATTY 3 cut(s) 610, 635, 1161
AsiGI ACCGGT 1 cut(s) 1075
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 2 cut(s) 192, 368
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 4 cut(s) 161, 347, 1099, 1191
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 996
BbvI GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BccI CCATC 4 cut(s) 68, 140, 374, 1128
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 3 cut(s) 39, 414, 431
BfaI CTAG 3 cut(s) 164, 926, 1242
BfmI CTRYAG 2 cut(s) 342, 1215
BfuAI ACCTGC 1 cut(s) 851
BisI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 7 cut(s) 262, 571, 664, 817, 820, 829, 1216
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 192, 368
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 788
BmsI GCATC 3 cut(s) 145, 523, 601
BmuI ACTGGG 1 cut(s) 788
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 3 cut(s) 484, 642, 1010
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 879
BpuMI CCSGG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 6, 100, 715
BsaWI WCCGGW 1 cut(s) 1075
BsaXI ACNNNNNCTCC 2 cut(s) 1171, 1201
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
Bse3DI GCAATG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 6, 100, 715
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 3 cut(s) 127, 741, 945
BseNI ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
BseRI GAGGAG 3 cut(s) 818, 1073, 1192
BseXI GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BseYI CCCAGC 1 cut(s) 586
BsgI GTGCAG 1 cut(s) 675
BshFI GGCC 3 cut(s) 5, 370, 745
BshTI ACCGGT 1 cut(s) 1075
BsiSI CCGG 3 cut(s) 102, 674, 1076
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 3 cut(s) 39, 414, 431
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 3 cut(s) 5, 370, 745
Bsp143I GATC 2 cut(s) 20, 1196
Bsp1720I GCTNAGC 1 cut(s) 113
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 4 cut(s) 816, 819, 858, 1157
BspANI GGCC 3 cut(s) 5, 370, 745
BspCNI CTCAG 3 cut(s) 126, 742, 946
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 1219
BspMI ACCTGC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 3 cut(s) 6, 100, 715
BssMI GATC 2 cut(s) 20, 1196
BssT1I CCWWGG 2 cut(s) 6, 715
Bst4CI ACNGT 7 cut(s) 61, 248, 296, 653, 934, 1000, 1225
Bst6I CTCTTC 1 cut(s) 1131
BstAPI GCANNNNNTGC 2 cut(s) 662, 827
BstC8I GCNNGC 2 cut(s) 1116, 1219
BstDEI CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 2 cut(s) 23, 1199
BstMAI GTCTC 3 cut(s) 39, 414, 431
BstMBI GATC 2 cut(s) 20, 1196
BstMWI GCNNNNNNNGC 6 cut(s) 139, 164, 662, 683, 824, 827
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 2 cut(s) 342, 1215
BstV1I GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BstV2I GAAGAC 1 cut(s) 996
BsuRI GGCC 3 cut(s) 5, 370, 745
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 7 cut(s) 57, 213, 292, 776, 986, 1005, 1014
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 1116, 1219
CaiI CAGNNNCTG 2 cut(s) 142, 662
CfoI GCGC 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 2 cut(s) 192, 368
Csp6I GTAC 1 cut(s) 783
CspAI ACCGGT 1 cut(s) 1075
CviAII CATG 5 cut(s) 7, 870, 910, 1025, 1205
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
DpnI GATC 2 cut(s) 22, 1198
DpnII GATC 2 cut(s) 20, 1196
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Eco130I CCWWGG 2 cut(s) 6, 715
Eco47I GGWCC 1 cut(s) 192
Eco57I CTGAAG 5 cut(s) 14, 119, 467, 549, 990
EcoRI GAATTC 2 cut(s) 635, 1161
EcoT14I CCWWGG 2 cut(s) 6, 715
EcoT22I ATGCAT 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 6, 715
FaeI CATG 5 cut(s) 10, 873, 913, 1028, 1208
FaqI GGGAC 1 cut(s) 205
FatI CATG 5 cut(s) 6, 869, 909, 1024, 1204
FauI CCCGC 1 cut(s) 1150
FauNDI CATATG 1 cut(s) 551
FblI GTMKAC 1 cut(s) 915
Fnu4HI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
FspBI CTAG 3 cut(s) 164, 926, 1242
GlaI GCGC 1 cut(s) 591
GluI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 3 cut(s) 484, 642, 1010
HaeIII GGCC 3 cut(s) 5, 370, 745
HapII CCGG 3 cut(s) 102, 674, 1076
HhaI GCGC 1 cut(s) 592
Hin1II CATG 5 cut(s) 10, 873, 913, 1028, 1208
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 2 cut(s) 883, 916
HindII GTYRAC 2 cut(s) 883, 916
HinfI GANTC 4 cut(s) 125, 762, 973, 983
HpaII CCGG 3 cut(s) 102, 674, 1076
HphI GGTGA 4 cut(s) 161, 347, 1099, 1191
Hpy166II GTNNAC 4 cut(s) 883, 916, 1012, 1128
Hpy188I TCNGA 7 cut(s) 130, 138, 547, 645, 955, 972, 1249
Hpy188III TCNNGA 4 cut(s) 287, 615, 977, 1187
Hpy8I GTNNAC 4 cut(s) 883, 916, 1012, 1128
HpyAV CCTTC 3 cut(s) 38, 977, 1204
HpyCH4III ACNGT 7 cut(s) 61, 248, 296, 653, 934, 1000, 1225
HpyCH4IV ACGT 2 cut(s) 450, 885
HpyCH4V TGCA 5 cut(s) 158, 188, 555, 656, 1217
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 164, 662, 683, 824, 827
HpyF3I CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
HpySE526I ACGT 2 cut(s) 450, 885
Hsp92II CATG 5 cut(s) 10, 873, 913, 1028, 1208
HspAI GCGC 1 cut(s) 590
Kzo9I GATC 2 cut(s) 20, 1196
LmnI GCTCC 1 cut(s) 1250
Lsp1109I GCAGC 5 cut(s) 247, 581, 649, 814, 1201
LweI GCATC 3 cut(s) 145, 523, 601
MaeI CTAG 3 cut(s) 164, 926, 1242
MaeII ACGT 2 cut(s) 450, 885
MaeIII GTNAC 5 cut(s) 55, 211, 697, 905, 1225
MalI GATC 2 cut(s) 22, 1198
MboI GATC 2 cut(s) 20, 1196
MboII GAAGA 6 cut(s) 164, 298, 487, 529, 996, 1148
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 570, 1033
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 557
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 273, 513, 1067
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 102, 674, 1076
MspR9I CCNGG 1 cut(s) 102
MwoI GCNNNNNNNGC 6 cut(s) 139, 164, 662, 683, 824, 827
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 551
NdeII GATC 2 cut(s) 20, 1196
NlaIII CATG 5 cut(s) 10, 873, 913, 1028, 1208
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 3 cut(s) 55, 211, 905
NsiI ATGCAT 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 851
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PfeI GAWTC 3 cut(s) 762, 973, 983
PflFI GACNNNGTC 1 cut(s) 911
PinAI ACCGGT 1 cut(s) 1075
PkrI GCNGC 7 cut(s) 262, 571, 664, 817, 820, 829, 1216
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp1406I AACGTT 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 2 cut(s) 192, 368
PstI CTGCAG 1 cut(s) 1219
PstNI CAGNNNCTG 2 cut(s) 142, 662
PsyI GACNNNGTC 1 cut(s) 911
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
RseI CAYNNNNRTG 1 cut(s) 218
SalI GTCGAC 1 cut(s) 914
SaqAI TTAA 3 cut(s) 273, 513, 1067
SatI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
Sau3AI GATC 2 cut(s) 20, 1196
Sau96I GGNCC 2 cut(s) 192, 368
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 102
SfaNI GCATC 3 cut(s) 145, 523, 601
SfcI CTRYAG 2 cut(s) 342, 1215
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 894
SmoI CTYRAG 1 cut(s) 894
Sse9I AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
SsiI CCGC 4 cut(s) 816, 819, 858, 1157
SspI AATATT 1 cut(s) 255
SspMI CTAG 3 cut(s) 164, 926, 1242
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 2 cut(s) 6, 715
TaaI ACNGT 7 cut(s) 61, 248, 296, 653, 934, 1000, 1225
TaiI ACGT 2 cut(s) 453, 888
TaqI TCGA 1 cut(s) 915
TasI AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
TauI GCSGC 2 cut(s) 818, 821
TfiI GAWTC 3 cut(s) 762, 973, 983
Tru1I TTAA 3 cut(s) 273, 513, 1067
Tru9I TTAA 3 cut(s) 273, 513, 1067
TscAI CASTG 7 cut(s) 64, 220, 299, 783, 993, 1005, 1014
TseFI GTSAC 3 cut(s) 55, 211, 905
TseI GCWGC 5 cut(s) 260, 569, 662, 827, 1214
Tsp45I GTSAC 3 cut(s) 55, 211, 905
TspDTI ATGAA 3 cut(s) 252, 858, 1149
TspRI CASTG 7 cut(s) 64, 220, 299, 783, 993, 1005, 1014
Tth111I GACNNNGTC 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 3 cut(s) 610, 635, 1161
XmiI GTMKAC 1 cut(s) 915
XspI CTAG 3 cut(s) 164, 926, 1242
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.