Lipolytic acyl hydrolase (LAH)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 98 |
| AciI | CCGC | 4 cut(s) | 98, 101, 219, 222 |
| AcsI | RAATTY | 1 cut(s) | 22 |
| AfaI | GTAC | 1 cut(s) | 70 |
| AgsI | TTSAA | 2 cut(s) | 22, 206 |
| AjnI | CCWGG | 1 cut(s) | 82 |
| AluBI | AGCT | 2 cut(s) | 144, 227 |
| AluI | AGCT | 2 cut(s) | 144, 227 |
| Alw21I | GWGCWC | 1 cut(s) | 229 |
| Alw26I | GTCTC | 1 cut(s) | 111 |
| AoxI | GGCC | 1 cut(s) | 80 |
| ApoI | RAATTY | 1 cut(s) | 22 |
| AspS9I | GGNCC | 2 cut(s) | 80, 193 |
| AsuII | TTCGAA | 1 cut(s) | 26 |
| AvaII | GGWCC | 1 cut(s) | 193 |
| BanII | GRGCYC | 2 cut(s) | 164, 229 |
| Bbv12I | GWGCWC | 1 cut(s) | 229 |
| BccI | CCATC | 1 cut(s) | 4 |
| BceAI | ACGGC | 1 cut(s) | 194 |
| BciT130I | CCWGG | 1 cut(s) | 84 |
| BcoDI | GTCTC | 1 cut(s) | 111 |
| BisI | GCNGC | 2 cut(s) | 99, 220 |
| BlpI | GCTNAGC | 1 cut(s) | 62 |
| BlsI | GCNGC | 2 cut(s) | 100, 221 |
| BmcAI | AGTACT | 1 cut(s) | 70 |
| Bme1390I | CCNGG | 1 cut(s) | 84 |
| Bme18I | GGWCC | 1 cut(s) | 193 |
| BmgT120I | GGNCC | 2 cut(s) | 80, 193 |
| BmiI | GGNNCC | 1 cut(s) | 161 |
| BmrFI | CCNGG | 1 cut(s) | 84 |
| BmrI | ACTGGG | 1 cut(s) | 81 |
| BmuI | ACTGGG | 1 cut(s) | 81 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 62 |
| Bpu14I | TTCGAA | 1 cut(s) | 26 |
| BsaJI | CCNNGG | 1 cut(s) | 83 |
| Bse1I | ACTGG | 1 cut(s) | 76 |
| Bse3DI | GCAATG | 1 cut(s) | 118 |
| BseBI | CCWGG | 1 cut(s) | 84 |
| BseDI | CCNNGG | 1 cut(s) | 83 |
| BseMI | GCAATG | 1 cut(s) | 118 |
| BseMII | CTCAG | 2 cut(s) | 53, 167 |
| BseNI | ACTGG | 1 cut(s) | 76 |
| Bsh1236I | CGCG | 1 cut(s) | 98 |
| BshFI | GGCC | 1 cut(s) | 82 |
| BsiHKAI | GWGCWC | 1 cut(s) | 229 |
| BslFI | GGGAC | 1 cut(s) | 64 |
| BsmAI | GTCTC | 1 cut(s) | 111 |
| BsmFI | GGGAC | 1 cut(s) | 64 |
| BsnI | GGCC | 1 cut(s) | 82 |
| Bsp119I | TTCGAA | 1 cut(s) | 26 |
| Bsp1286I | GDGCHC | 2 cut(s) | 164, 229 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 62 |
| BspACI | CCGC | 4 cut(s) | 98, 101, 219, 222 |
| BspANI | GGCC | 1 cut(s) | 82 |
| BspCNI | CTCAG | 2 cut(s) | 54, 166 |
| BspFNI | CGCG | 1 cut(s) | 98 |
| BspLI | GGNNCC | 1 cut(s) | 161 |
| BspT104I | TTCGAA | 1 cut(s) | 26 |
| BsrDI | GCAATG | 1 cut(s) | 118 |
| BsrI | ACTGG | 1 cut(s) | 76 |
| BssECI | CCNNGG | 1 cut(s) | 83 |
| Bst2UI | CCWGG | 1 cut(s) | 84 |
| Bst4CI | ACNGT | 1 cut(s) | 57 |
| Bst6I | CTCTTC | 1 cut(s) | 83 |
| BstBI | TTCGAA | 1 cut(s) | 26 |
| BstC8I | GCNNGC | 1 cut(s) | 217 |
| BstDEI | CTNAG | 2 cut(s) | 62, 153 |
| BstFNI | CGCG | 1 cut(s) | 98 |
| BstMAI | GTCTC | 1 cut(s) | 111 |
| BstNI | CCWGG | 1 cut(s) | 84 |
| BstSCI | CCNGG | 1 cut(s) | 82 |
| BstUI | CGCG | 1 cut(s) | 98 |
| BsuRI | GGCC | 1 cut(s) | 82 |
| BtsIMutI | CAGTG | 1 cut(s) | 161 |
| Cac8I | GCNNGC | 1 cut(s) | 217 |
| Cfr13I | GGNCC | 2 cut(s) | 80, 193 |
| Csp6I | GTAC | 1 cut(s) | 69 |
| CviJI | RGCY | 8 cut(s) | 61, 82, 130, 144, 162, 201, 209, 227 |
| CviKI_1 | RGCY | 8 cut(s) | 61, 82, 130, 144, 162, 201, 209, 227 |
| CviQI | GTAC | 1 cut(s) | 69 |
| DdeI | CTNAG | 2 cut(s) | 62, 153 |
| Eam1104I | CTCTTC | 1 cut(s) | 83 |
| EarI | CTCTTC | 1 cut(s) | 83 |
| EciI | GGCGGA | 1 cut(s) | 237 |
| Ecl136II | GAGCTC | 1 cut(s) | 227 |
| Eco24I | GRGCYC | 2 cut(s) | 164, 229 |
| Eco47I | GGWCC | 1 cut(s) | 193 |
| Eco53kI | GAGCTC | 1 cut(s) | 227 |
| EcoICRI | GAGCTC | 1 cut(s) | 227 |
| EcoRI | GAATTC | 1 cut(s) | 22 |
| EcoRII | CCWGG | 1 cut(s) | 82 |
| EcoT38I | GRGCYC | 2 cut(s) | 164, 229 |
| FaiI | YATR | 1 cut(s) | 244 |
| FaqI | GGGAC | 1 cut(s) | 64 |
| Fnu4HI | GCNGC | 2 cut(s) | 99, 220 |
| FriOI | GRGCYC | 2 cut(s) | 164, 229 |
| Fsp4HI | GCNGC | 2 cut(s) | 99, 220 |
| GluI | GCNGC | 2 cut(s) | 99, 220 |
| HaeIII | GGCC | 1 cut(s) | 82 |
| HinfI | GANTC | 1 cut(s) | 149 |
| Hpy188I | TCNGA | 2 cut(s) | 44, 134 |
| Hpy188III | TCNNGA | 1 cut(s) | 191 |
| HpyCH4III | ACNGT | 1 cut(s) | 57 |
| HpyCH4V | TGCA | 1 cut(s) | 185 |
| HpyF3I | CTNAG | 2 cut(s) | 62, 153 |
| LmnI | GCTCC | 3 cut(s) | 141, 159, 224 |
| LpnPI | CCDG | 4 cut(s) | 57, 69, 96, 176 |
| MboII | GAAGA | 1 cut(s) | 100 |
| MhlI | GDGCHC | 2 cut(s) | 164, 229 |
| MluCI | AATT | 2 cut(s) | 22, 29 |
| MlyI | GAGTC | 1 cut(s) | 143 |
| MnlI | CCTC | 3 cut(s) | 84, 120, 180 |
| MspR9I | CCNGG | 1 cut(s) | 84 |
| MvaI | CCWGG | 1 cut(s) | 84 |
| MvnI | CGCG | 1 cut(s) | 98 |
| NlaIV | GGNNCC | 1 cut(s) | 161 |
| NspV | TTCGAA | 1 cut(s) | 26 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 11 |
| PkrI | GCNGC | 2 cut(s) | 100, 221 |
| PleI | GAGTC | 1 cut(s) | 143 |
| PpsI | GAGTC | 1 cut(s) | 143 |
| Psp124BI | GAGCTC | 1 cut(s) | 229 |
| Psp6I | CCWGG | 1 cut(s) | 82 |
| PspGI | CCWGG | 1 cut(s) | 82 |
| PspN4I | GGNNCC | 1 cut(s) | 161 |
| PspPI | GGNCC | 2 cut(s) | 80, 193 |
| RsaI | GTAC | 1 cut(s) | 70 |
| RsaNI | GTAC | 1 cut(s) | 69 |
| SacI | GAGCTC | 1 cut(s) | 229 |
| SatI | GCNGC | 2 cut(s) | 99, 220 |
| Sau96I | GGNCC | 2 cut(s) | 80, 193 |
| ScaI | AGTACT | 1 cut(s) | 70 |
| SchI | GAGTC | 1 cut(s) | 143 |
| ScrFI | CCNGG | 1 cut(s) | 84 |
| SduI | GDGCHC | 2 cut(s) | 164, 229 |
| SetI | ASST | 2 cut(s) | 146, 229 |
| SfuI | TTCGAA | 1 cut(s) | 26 |
| SinI | GGWCC | 1 cut(s) | 193 |
| Sse9I | AATT | 2 cut(s) | 22, 29 |
| SsiI | CCGC | 4 cut(s) | 98, 101, 219, 222 |
| SstI | GAGCTC | 1 cut(s) | 229 |
| StyD4I | CCNGG | 1 cut(s) | 82 |
| TaaI | ACNGT | 1 cut(s) | 57 |
| TaqI | TCGA | 1 cut(s) | 26 |
| TasI | AATT | 2 cut(s) | 22, 29 |
| TatI | WGTACW | 1 cut(s) | 68 |
| TauI | GCSGC | 2 cut(s) | 101, 222 |
| TscAI | CASTG | 1 cut(s) | 161 |
| TspRI | CASTG | 1 cut(s) | 161 |
| VpaK11BI | GGWCC | 1 cut(s) | 193 |
| XapI | RAATTY | 1 cut(s) | 22 |
| ZrmI | AGTACT | 1 cut(s) | 70 |