Prupe.1G439800_v2.0.a1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000265
Physical Location & Seq
Reverse (-)
37388164 .. 37390544
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G439800.1

Sequence Viewer

Length: 1233 bp
ATGAACAGAAGCAAGAGTATACCTTTGCAGGCCCCAACCTATGGAAACCTCATCACCGTTTTAAGCATCGATGGGGGTGGAATAAGAGGGCTTATCCCTGGAACCATCCTTGGTTACCTTGAAGCTGAGCTTCAGAAACTGGATGGTGAAGATGCAAGAATCGCTGACTATTTCGATGTGATTGCAGGAACAAGCACTGGTGGCCTTGTAACTGCCATGCTTGCAACCCCAGACGAGAACAACCGCCCTGTGTTTGCTGCCAAAGATATTAAGGACTTCTACCTTACTCACTGCCCCAAAATCTTCCCCCAGAAACACTGTCCAATATTTCCTTATACTACAAAGATTATCAAAGCTCTAGCAGGACCAAAATACGATGGGAAGTATTTGCATGACTTGGTTGGGCAGAAACTTGGTGACAAAAAACTGCACCAGACATTAACTAACGTTGTCATTCCAACATTTGACATAAAGCAGCTCCAACCAACAATCTTTTCCAGCTATGAGGTGAAACATAAGCCAGGCTTCGATGCTAAACTCTCAGACATATGCATTGCAACCTCAGCAGCACCTACTTATCTCCCAGCCCATTATTTTGAAACCATGGACCCAGAAGGGAATGTAAGAGAATTTAACCTCATCGATGGTGGTGTGGCTGCAAATAATCCGACTTTACTTGCTATTGGTGAAGTGACAAAGACAATAATCAAGGGGAGCTCAGATTTTTTCCCTATAAAGCCCATGGACTATGCAAGGTTTCTAGTGATATCATTAGGAACAGGCTCAGCAAAAGCTGAGTTCAAATACCATGCACTTTCTGCAGCCAAGTGGGGCTTGTTAGATTGGTTAACCAGCGGTGGTGGAACCCCAATAATTGATGTTTTCAGTCAATCAAGTGCTGATATGGTCGATTATCACCTTTCTGTTGTTTTCCAAGCCCTCCACTCAGAAAATAATTACCTTCGAATTCAGGATGACACGTTAACCGGGCATGTATCTTCTGTGGACATAGCCGATAAGAAGAATTTGGATGATCTTCTGATAGTTGGTGAAGGCTTGTTGAAAAAACCAGTTTCCAGGGTTAATTTGGAGACTGGCAGGGTAGAGCCTTCAAAACATGAGTCTAATGAAGAGGCTCTCACAAGGTTTGCAAAACTATTATCGGAAGAGAAGTGGCTTCGCCTTGCAAGGTCACCCCACGGACATACTCCAAAACCAAAACCCAACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

411

Amino Acids

45.01

Weight (kDa)

6.5

Isoelectric Point (pI)

27.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 41
AccI GTMKAC 1 cut(s) 19
AciI CCGC 2 cut(s) 244, 855
AclI AACGTT 1 cut(s) 447
AcsI RAATTY 3 cut(s) 629, 966, 1024
AcuI CTGAAG 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 41
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 5 cut(s) 122, 599, 802, 1063, 1113
AjnI CCWGG 3 cut(s) 97, 520, 1076
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 7 cut(s) 125, 130, 356, 478, 501, 717, 794
AluI AGCT 7 cut(s) 125, 130, 356, 478, 501, 717, 794
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 1 cut(s) 1085
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 2 cut(s) 30, 202
ApeKI GCWGC 5 cut(s) 257, 475, 566, 656, 821
ApoI RAATTY 3 cut(s) 629, 966, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
AspS9I GGNCC 3 cut(s) 31, 365, 607
AsuC2I CCSGG 1 cut(s) 988
AsuHPI GGTGA 8 cut(s) 46, 158, 428, 520, 698, 908, 1061, 1185
AsuII TTCGAA 1 cut(s) 964
AvaII GGWCC 2 cut(s) 365, 607
BanII GRGCYC 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 5 cut(s) 244, 487, 578, 643, 833
BccI CCATC 5 cut(s) 65, 113, 137, 371, 638
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 3 cut(s) 99, 522, 1078
BcnI CCSGG 1 cut(s) 988
BcoDI GTCTC 1 cut(s) 1085
BfaI CTAG 2 cut(s) 359, 761
BfmI CTRYAG 1 cut(s) 819
BisI GCNGC 5 cut(s) 258, 476, 567, 657, 822
BlpI GCTNAGC 2 cut(s) 126, 784
BlsI GCNGC 5 cut(s) 259, 477, 568, 658, 823
Bme1390I CCNGG 4 cut(s) 99, 522, 988, 1078
Bme18I GGWCC 2 cut(s) 365, 607
BmgT120I GGNCC 3 cut(s) 31, 365, 607
BmiI GGNNCC 4 cut(s) 33, 103, 609, 865
BmrFI CCNGG 4 cut(s) 99, 522, 988, 1078
BmsI GCATC 3 cut(s) 75, 142, 520
Bpu10I CCTNAGC 1 cut(s) 562
Bpu1102I GCTNAGC 2 cut(s) 126, 784
Bpu14I TTCGAA 1 cut(s) 964
BpuMI CCSGG 1 cut(s) 988
Bsa29I ATCGAT 2 cut(s) 69, 642
BsaJI CCNNGG 6 cut(s) 97, 109, 603, 741, 1077, 1198
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 4 cut(s) 144, 202, 1070, 1099
Bse3DI GCAATG 1 cut(s) 552
BseBI CCWGG 3 cut(s) 99, 522, 1078
BseCI ATCGAT 2 cut(s) 69, 642
BseDI CCNNGG 6 cut(s) 97, 109, 603, 741, 1077, 1198
BseGI GGATG 4 cut(s) 105, 148, 979, 1036
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 7 cut(s) 117, 555, 576, 732, 786, 798, 960
BseNI ACTGG 4 cut(s) 144, 202, 1070, 1099
BseXI GCAGC 5 cut(s) 244, 487, 578, 643, 833
BseYI CCCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 2 cut(s) 32, 204
BshVI ATCGAT 2 cut(s) 69, 642
BsiHKAI GWGCWC 1 cut(s) 719
BsiSI CCGG 1 cut(s) 987
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 1 cut(s) 1085
BsnI GGCC 2 cut(s) 32, 204
Bsp119I TTCGAA 1 cut(s) 964
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 1 cut(s) 1033
Bsp1720I GCTNAGC 2 cut(s) 126, 784
Bsp19I CCATGG 2 cut(s) 603, 741
BspACI CCGC 2 cut(s) 244, 855
BspANI GGCC 2 cut(s) 32, 204
BspCNI CTCAG 7 cut(s) 118, 554, 575, 731, 787, 797, 959
BspDI ATCGAT 2 cut(s) 69, 642
BspLI GGNNCC 4 cut(s) 33, 103, 609, 865
BspMAI CTGCAG 1 cut(s) 823
BspT104I TTCGAA 1 cut(s) 964
BsrDI GCAATG 1 cut(s) 552
BsrI ACTGG 4 cut(s) 144, 202, 1070, 1099
BssECI CCNNGG 6 cut(s) 97, 109, 603, 741, 1077, 1198
BssMI GATC 1 cut(s) 1033
BssNAI GTATAC 1 cut(s) 20
BssT1I CCWWGG 3 cut(s) 109, 603, 741
Bst1107I GTATAC 1 cut(s) 20
Bst2UI CCWGG 3 cut(s) 99, 522, 1078
Bst4CI ACNGT 2 cut(s) 58, 320
Bst6I CTCTTC 2 cut(s) 1125, 1161
BstAPI GCANNNNNTGC 1 cut(s) 818
BstBI TTCGAA 1 cut(s) 964
BstC8I GCNNGC 2 cut(s) 30, 222
BstDEI CTNAG 7 cut(s) 126, 541, 562, 718, 784, 795, 946
BstDSI CCRYGG 3 cut(s) 603, 741, 1198
BstEII GGTNACC 2 cut(s) 113, 1191
BstF5I GGATG 4 cut(s) 105, 148, 979, 1036
BstKTI GATC 1 cut(s) 1036
BstMAI GTCTC 1 cut(s) 1085
BstMBI GATC 1 cut(s) 1033
BstMWI GCNNNNNNNGC 5 cut(s) 161, 201, 221, 563, 818
BstNI CCWGG 3 cut(s) 99, 522, 1078
BstNSI RCATGY 1 cut(s) 995
BstPI GGTNACC 2 cut(s) 113, 1191
BstSCI CCNGG 4 cut(s) 97, 520, 986, 1076
BstSFI CTRYAG 1 cut(s) 819
BstV1I GCAGC 5 cut(s) 244, 487, 578, 643, 833
BstZ17I GTATAC 1 cut(s) 20
Bsu15I ATCGAT 2 cut(s) 69, 642
BsuRI GGCC 2 cut(s) 32, 204
BsuTUI ATCGAT 2 cut(s) 69, 642
BtgI CCRYGG 3 cut(s) 603, 741, 1198
BtsCI GGATG 4 cut(s) 105, 148, 979, 1036
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 3 cut(s) 195, 289, 316
Cac8I GCNNGC 2 cut(s) 30, 222
CaiI CAGNNNCTG 1 cut(s) 139
Cfr13I GGNCC 3 cut(s) 31, 365, 607
ClaI ATCGAT 2 cut(s) 69, 642
CviAII CATG 7 cut(s) 217, 392, 604, 742, 809, 992, 1118
DdeI CTNAG 7 cut(s) 126, 541, 562, 718, 784, 795, 946
DpnI GATC 1 cut(s) 1035
DpnII GATC 1 cut(s) 1033
Eam1104I CTCTTC 2 cut(s) 1125, 1161
EarI CTCTTC 2 cut(s) 1125, 1161
Ecl136II GAGCTC 1 cut(s) 717
Eco130I CCWWGG 3 cut(s) 109, 603, 741
Eco24I GRGCYC 1 cut(s) 719
Eco32I GATATC 1 cut(s) 768
Eco47I GGWCC 2 cut(s) 365, 607
Eco53kI GAGCTC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 116
Eco91I GGTNACC 2 cut(s) 113, 1191
EcoICRI GAGCTC 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 31
EcoO65I GGTNACC 2 cut(s) 113, 1191
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 3 cut(s) 97, 520, 1076
EcoRV GATATC 1 cut(s) 768
EcoT14I CCWWGG 3 cut(s) 109, 603, 741
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 3 cut(s) 109, 603, 741
FaeI CATG 7 cut(s) 220, 395, 607, 745, 812, 995, 1121
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 509, 541, 818, 850
FatI CATG 7 cut(s) 216, 391, 603, 741, 808, 991, 1117
FauNDI CATATG 1 cut(s) 548
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 5 cut(s) 258, 476, 567, 657, 822
FokI GGATG 4 cut(s) 92, 155, 986, 1043
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 5 cut(s) 258, 476, 567, 657, 822
FspBI CTAG 2 cut(s) 359, 761
GluI GCNGC 5 cut(s) 258, 476, 567, 657, 822
GsaI CCCAGC 1 cut(s) 587
HaeIII GGCC 2 cut(s) 32, 204
HapII CCGG 1 cut(s) 987
Hin1II CATG 7 cut(s) 220, 395, 607, 745, 812, 995, 1121
HincII GTYRAC 2 cut(s) 849, 984
HindII GTYRAC 2 cut(s) 849, 984
HinfI GANTC 2 cut(s) 159, 1121
HpaI GTTAAC 2 cut(s) 849, 984
HpaII CCGG 1 cut(s) 987
HphI GGTGA 8 cut(s) 46, 158, 428, 520, 698, 908, 1061, 1185
Hpy166II GTNNAC 4 cut(s) 20, 849, 984, 1006
Hpy188I TCNGA 7 cut(s) 135, 544, 669, 721, 949, 1041, 1165
Hpy188III TCNNGA 1 cut(s) 971
Hpy8I GTNNAC 4 cut(s) 20, 849, 984, 1006
HpyAV CCTTC 4 cut(s) 608, 971, 1046, 1119
HpyCH4III ACNGT 2 cut(s) 58, 320
HpyCH4IV ACGT 2 cut(s) 447, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 201, 221, 563, 818
HpyF3I CTNAG 7 cut(s) 126, 541, 562, 718, 784, 795, 946
HpySE526I ACGT 2 cut(s) 447, 980
Hsp92II CATG 7 cut(s) 220, 395, 607, 745, 812, 995, 1121
KspAI GTTAAC 2 cut(s) 849, 984
Kzo9I GATC 1 cut(s) 1033
LmnI GCTCC 2 cut(s) 483, 714
Lsp1109I GCAGC 5 cut(s) 244, 487, 578, 643, 833
LweI GCATC 3 cut(s) 75, 142, 520
MaeI CTAG 2 cut(s) 359, 761
MaeII ACGT 2 cut(s) 447, 980
MaeIII GTNAC 5 cut(s) 113, 208, 416, 691, 1191
MalI GATC 1 cut(s) 1035
MboI GATC 1 cut(s) 1033
MboII GAAGA 7 cut(s) 161, 295, 990, 1028, 1033, 1142, 1178
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 6 cut(s) 629, 873, 955, 966, 1024, 1084
MlyI GAGTC 1 cut(s) 1130
MmeI TCCRAC 3 cut(s) 482, 505, 692
MnlI CCTC 7 cut(s) 59, 80, 499, 571, 647, 950, 1126
Mph1103I ATGCAT 1 cut(s) 554
MseI TTAA 7 cut(s) 62, 270, 440, 633, 848, 983, 1083
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 1 cut(s) 987
MspR9I CCNGG 4 cut(s) 99, 522, 988, 1078
MvaI CCWGG 3 cut(s) 99, 522, 1078
MwoI GCNNNNNNNGC 5 cut(s) 161, 201, 221, 563, 818
NciI CCSGG 1 cut(s) 988
NcoI CCATGG 2 cut(s) 603, 741
NdeI CATATG 1 cut(s) 548
NdeII GATC 1 cut(s) 1033
NlaIII CATG 7 cut(s) 220, 395, 607, 745, 812, 995, 1121
NlaIV GGNNCC 4 cut(s) 33, 103, 609, 865
NmuCI GTSAC 3 cut(s) 416, 691, 1191
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 1 cut(s) 964
PfeI GAWTC 1 cut(s) 159
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 5 cut(s) 259, 477, 568, 658, 823
PleI GAGTC 1 cut(s) 1129
PpsI GAGTC 1 cut(s) 1129
Psp124BI GAGCTC 1 cut(s) 719
Psp1406I AACGTT 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 97, 520, 1076
PspEI GGTNACC 2 cut(s) 113, 1191
PspFI CCCAGC 1 cut(s) 583
PspGI CCWGG 3 cut(s) 97, 520, 1076
PspN4I GGNNCC 4 cut(s) 33, 103, 609, 865
PspPI GGNCC 3 cut(s) 31, 365, 607
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 139
SacI GAGCTC 1 cut(s) 719
SaqAI TTAA 7 cut(s) 62, 270, 440, 633, 848, 983, 1083
SatI GCNGC 5 cut(s) 258, 476, 567, 657, 822
Sau3AI GATC 1 cut(s) 1033
Sau96I GGNCC 3 cut(s) 31, 365, 607
SchI GAGTC 1 cut(s) 1130
ScrFI CCNGG 4 cut(s) 99, 522, 988, 1078
SduI GDGCHC 1 cut(s) 719
SfaNI GCATC 3 cut(s) 75, 142, 520
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 964
SinI GGWCC 2 cut(s) 365, 607
Sse9I AATT 6 cut(s) 629, 873, 955, 966, 1024, 1084
SsiI CCGC 2 cut(s) 244, 855
SspI AATATT 1 cut(s) 327
SspMI CTAG 2 cut(s) 359, 761
SstI GAGCTC 1 cut(s) 719
StyD4I CCNGG 4 cut(s) 97, 520, 986, 1076
StyI CCWWGG 3 cut(s) 109, 603, 741
TaaI ACNGT 2 cut(s) 58, 320
TaiI ACGT 2 cut(s) 450, 983
TaqI TCGA 6 cut(s) 69, 174, 528, 642, 909, 964
TasI AATT 6 cut(s) 629, 873, 955, 966, 1024, 1084
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 7 cut(s) 62, 270, 440, 633, 848, 983, 1083
Tru9I TTAA 7 cut(s) 62, 270, 440, 633, 848, 983, 1083
TscAI CASTG 3 cut(s) 202, 296, 323
TseFI GTSAC 3 cut(s) 416, 691, 1191
TseI GCWGC 5 cut(s) 257, 475, 566, 656, 821
Tsp45I GTSAC 3 cut(s) 416, 691, 1191
TspDTI ATGAA 2 cut(s) 17, 1143
TspGWI ACGGA 1 cut(s) 1215
TspRI CASTG 3 cut(s) 202, 296, 323
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 365, 607
XapI RAATTY 3 cut(s) 629, 966, 1024
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 1 cut(s) 1084
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 2 cut(s) 359, 761
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.