FvH4_4g24280

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000265
Physical Location & Seq
Forward (+)
26520652 .. 26522807
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24280.t2

Sequence Viewer

Length: 1224 bp
ATGGAAAGAACTAGTAGTTTCGCTATGCAGCCCCCAACCTTCGGAAACCTAATCACTGTTCTGAGCATTGACGGCGGTGGAATAAGAGGGATTATCCCTGGAACCATCCTTGCTTTCCTTGAGTCTGAACTTCAGATGCTGGATGGTGAAAATGCAAGACTCGCAGACTATTTCGATGTGATTTCAGGGACAAGCACAGGTGGTCTTGTAACAGCCATGCTCACAGCTCCAAATGAGAATAACCGTCCACTATTTGCTGCCAAAGATATCAAGGACTTCTACCTTACGCACTCCCCCAAAATCTTCCCCCAGAAGAGTGGTTGGCTGTTTCCTCGTGCTAGAAAGATCATCAAAGCTCTAGCAGGACCCAAATACGATGGCAAGTATCTGCACGGCTTGGTTAGGGAAAAACTTGGTGACAAGAAACTGAACCAAACGCTGACTAATGTTGTCATTCCTGCATTTGACATAAAGAATCTCCAGCCAGCTATCTTTTCTAGCTTCGAGGTGAAAAATAAGCCTACATTTGATGCCCTACTCTCGGACATATGTATTGCAACCTCAGCGGCACCAACTTATCTTCCAGCTCATTTTTTTAAGACCAACGGCCTCAAAGGAGAAGTTAGAGAGTTTCATCTGATTGATGGTGGATTGGCAGCAAATAATCCAACTTTGGTTGCTATTGGTGAAGTAACGAAGGAAATAATTAAGGGGAGTTCAGACTTCTTTCCTATAAAACCAATGGACTATGGACGATTTCTGGTGATATCACTAGGAACTGGTTCATCGAAAGCTGAACTCAAATACAATGCTCATGTTGCCTCTAAATGGGGTGTCTTGAATTGGTTAACCAGCGGTGGTTCCACCCCATTGATCGATGCTTTCAGTCAAGCAAGTGCTGATATGGTCGATTTGCATCTTTCTGCGGTCTTTAAAGCCCTTCACTCGGAGAAAAACTATCTTCGAATTCAGGATGACACACTGAGTGGCGATGTATCTTCTGTTGATATAGCAACACAGAAGAATCTGGACCGTCTACTCAAAGTTGGTAACGGACTATTGAAGCAACCAGTCTCCAGGGTTAATTTAGAGACGGGCAAATTCGAAGCTTGTAATCATGAGACTAATGCCGAGGCTCTTAGAAGGTTTGCAAAATTGCTATCTGCAGAGAAATGTCTTCGCCTCGCAAGGTCTCCCCATGGACATGTTGCAAAGTCCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

408

Amino Acids

44.35

Weight (kDa)

9.1

Isoelectric Point (pI)

23.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 228 8.4e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 1036
AciI CCGC 4 cut(s) 75, 566, 855, 926
AcsI RAATTY 2 cut(s) 966, 1100
AcuI CTGAAG 1 cut(s) 116
AdeI CACNNNGTG 1 cut(s) 986
AfiI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
AflIII ACRYGT 1 cut(s) 1204
AgsI TTSAA 2 cut(s) 841, 1063
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 97, 1076
AluBI AGCT 7 cut(s) 227, 356, 488, 501, 587, 794, 1109
AluI AGCT 7 cut(s) 227, 356, 488, 501, 587, 794, 1109
Alw26I GTCTC 4 cut(s) 1078, 1085, 1115, 1197
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 1 cut(s) 607
ApeKI GCWGC 3 cut(s) 28, 257, 656
ApoI RAATTY 2 cut(s) 966, 1100
Asp700I GAANNNNTTC 1 cut(s) 781
AspS9I GGNCC 2 cut(s) 365, 1030
AsuHPI GGTGA 5 cut(s) 158, 428, 520, 698, 775
AsuII TTCGAA 2 cut(s) 964, 1104
AvaII GGWCC 2 cut(s) 365, 1030
BanI GGYRCC 1 cut(s) 568
BauI CACGAG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 1169
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 3 cut(s) 40, 244, 668
BccI CCATC 4 cut(s) 113, 137, 371, 638
BceAI ACGGC 3 cut(s) 88, 409, 622
BciT130I CCWGG 2 cut(s) 99, 1078
BcoDI GTCTC 4 cut(s) 1078, 1085, 1115, 1197
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 5 cut(s) 12, 339, 359, 498, 773
BfmI CTRYAG 1 cut(s) 1164
BisI GCNGC 4 cut(s) 29, 258, 567, 657
BlsI GCNGC 4 cut(s) 30, 259, 568, 658
Bme1390I CCNGG 2 cut(s) 99, 1078
Bme18I GGWCC 2 cut(s) 365, 1030
BmgT120I GGNCC 2 cut(s) 365, 1030
BmiI GGNNCC 4 cut(s) 103, 367, 570, 862
BmrFI CCNGG 2 cut(s) 99, 1078
BmsI GCATC 4 cut(s) 126, 520, 868, 925
BpiI GAAGAC 1 cut(s) 1169
BpmI CTGGAG 2 cut(s) 464, 1060
Bpu10I CCTNAGC 1 cut(s) 562
Bpu14I TTCGAA 2 cut(s) 964, 1104
BpuEI CTTGAG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 876
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 1197
BsaJI CCNNGG 4 cut(s) 97, 1077, 1131, 1198
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
Bse1I ACTGG 2 cut(s) 784, 1070
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 99, 1078
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 4 cut(s) 97, 1077, 1131, 1198
BseGI GGATG 3 cut(s) 105, 148, 979
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
BseMII CTCAG 3 cut(s) 53, 576, 974
BseNI ACTGG 2 cut(s) 784, 1070
BseXI GCAGC 3 cut(s) 40, 244, 668
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 1 cut(s) 609
BshNI GGYRCC 1 cut(s) 568
BshVI ATCGAT 1 cut(s) 876
BslFI GGGAC 2 cut(s) 202, 1201
BslI CCNNNNNNNGG 4 cut(s) 41, 541, 828, 946
BsmAI GTCTC 4 cut(s) 1078, 1085, 1115, 1197
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 2 cut(s) 202, 1201
BsnI GGCC 1 cut(s) 609
Bso31I GGTCTC 1 cut(s) 1197
Bsp119I TTCGAA 2 cut(s) 964, 1104
Bsp143I GATC 2 cut(s) 345, 873
Bsp19I CCATGG 1 cut(s) 1198
BspACI CCGC 4 cut(s) 75, 566, 855, 926
BspANI GGCC 1 cut(s) 609
BspCNI CTCAG 3 cut(s) 54, 575, 975
BspDI ATCGAT 1 cut(s) 876
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 4 cut(s) 103, 367, 570, 862
BspMAI CTGCAG 1 cut(s) 1168
BspT104I TTCGAA 2 cut(s) 964, 1104
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 1197
BsrI ACTGG 2 cut(s) 784, 1070
BssECI CCNNGG 4 cut(s) 97, 1077, 1131, 1198
BssMI GATC 2 cut(s) 345, 873
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 1198
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 99, 1078
Bst4CI ACNGT 3 cut(s) 58, 245, 1034
Bst6I CTCTTC 1 cut(s) 308
BstBI TTCGAA 2 cut(s) 964, 1104
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 4 cut(s) 62, 562, 983, 1139
BstDSI CCRYGG 1 cut(s) 1198
BstF5I GGATG 3 cut(s) 105, 148, 979
BstKTI GATC 2 cut(s) 348, 876
BstMAI GTCTC 4 cut(s) 1078, 1085, 1115, 1197
BstMBI GATC 2 cut(s) 345, 873
BstMWI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
BstNI CCWGG 2 cut(s) 99, 1078
BstNSI RCATGY 1 cut(s) 1208
BstSCI CCNGG 2 cut(s) 97, 1076
BstSFI CTRYAG 1 cut(s) 1164
BstV1I GCAGC 3 cut(s) 40, 244, 668
BstV2I GAAGAC 1 cut(s) 1169
BstXI CCANNNNNNTGG 1 cut(s) 317
Bsu15I ATCGAT 1 cut(s) 876
BsuRI GGCC 1 cut(s) 609
BsuTUI ATCGAT 1 cut(s) 876
BtgI CCRYGG 1 cut(s) 1198
BtgZI GCGATG 1 cut(s) 1005
BtsCI GGATG 3 cut(s) 105, 148, 979
BtsIMutI CAGTG 3 cut(s) 54, 980, 1219
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 1117
Cfr13I GGNCC 2 cut(s) 365, 1030
ClaI ATCGAT 1 cut(s) 876
CviAII CATG 5 cut(s) 217, 815, 1118, 1199, 1205
DdeI CTNAG 4 cut(s) 62, 562, 983, 1139
DpnI GATC 2 cut(s) 347, 875
DpnII GATC 2 cut(s) 345, 873
DraI TTTAAA 1 cut(s) 934
DraIII CACNNNGTG 1 cut(s) 986
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 1198
Eco31I GGTCTC 1 cut(s) 1197
Eco32I GATATC 2 cut(s) 268, 768
Eco47I GGWCC 2 cut(s) 365, 1030
Eco57I CTGAAG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 365
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 2 cut(s) 97, 1076
EcoRV GATATC 2 cut(s) 268, 768
EcoT14I CCWWGG 1 cut(s) 1198
ErhI CCWWGG 1 cut(s) 1198
Esp3I CGTCTC 1 cut(s) 1085
FaeI CATG 5 cut(s) 220, 818, 1121, 1202, 1208
FaqI GGGAC 2 cut(s) 202, 1201
FatI CATG 5 cut(s) 216, 814, 1117, 1198, 1204
FauNDI CATATG 1 cut(s) 548
FblI GTMKAC 1 cut(s) 1036
Fnu4HI GCNGC 4 cut(s) 29, 258, 567, 657
FokI GGATG 3 cut(s) 92, 155, 986
Fsp4HI GCNGC 4 cut(s) 29, 258, 567, 657
FspBI CTAG 5 cut(s) 12, 339, 359, 498, 773
GluI GCNGC 4 cut(s) 29, 258, 567, 657
GsuI CTGGAG 2 cut(s) 464, 1060
HaeIII GGCC 1 cut(s) 609
Hin1II CATG 5 cut(s) 220, 818, 1121, 1202, 1208
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HindIII AAGCTT 1 cut(s) 1107
HinfI GANTC 4 cut(s) 122, 159, 475, 1024
HpaI GTTAAC 1 cut(s) 849
HphI GGTGA 5 cut(s) 158, 428, 520, 698, 775
Hpy166II GTNNAC 3 cut(s) 248, 849, 1037
Hpy188I TCNGA 8 cut(s) 44, 63, 127, 135, 544, 639, 721, 949
Hpy188III TCNNGA 4 cut(s) 838, 971, 1028, 1118
Hpy8I GTNNAC 3 cut(s) 248, 849, 1037
HpyAV CCTTC 4 cut(s) 49, 691, 950, 1137
HpyCH4III ACNGT 3 cut(s) 58, 245, 1034
HpyCH4V TGCA 9 cut(s) 28, 155, 391, 461, 557, 916, 1151, 1166, 1211
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
HpyF3I CTNAG 4 cut(s) 62, 562, 983, 1139
Hsp92II CATG 5 cut(s) 220, 818, 1121, 1202, 1208
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 2 cut(s) 345, 873
LmnI GCTCC 1 cut(s) 232
Lsp1109I GCAGC 3 cut(s) 40, 244, 668
LweI GCATC 4 cut(s) 126, 520, 868, 925
MaeI CTAG 5 cut(s) 12, 339, 359, 498, 773
MaeIII GTNAC 4 cut(s) 208, 416, 691, 1049
MalI GATC 2 cut(s) 347, 875
MboI GATC 2 cut(s) 345, 873
MboII GAAGA 7 cut(s) 295, 325, 572, 953, 990, 1033, 1169
MluCI AATT 6 cut(s) 705, 841, 966, 1084, 1100, 1154
MlyI GAGTC 2 cut(s) 131, 153
MmeI TCCRAC 1 cut(s) 692
MnlI CCTC 8 cut(s) 80, 342, 499, 571, 620, 832, 1126, 1193
MroXI GAANNNNTTC 1 cut(s) 781
MseI TTAA 5 cut(s) 597, 708, 848, 933, 1083
MslI CAYNNNNRTG 1 cut(s) 1203
MspA1I CMGCKG 2 cut(s) 566, 855
MspR9I CCNGG 2 cut(s) 99, 1078
MvaI CCWGG 2 cut(s) 99, 1078
MwoI GCNNNNNNNGC 4 cut(s) 72, 161, 563, 818
NcoI CCATGG 1 cut(s) 1198
NdeI CATATG 1 cut(s) 548
NdeII GATC 2 cut(s) 345, 873
NlaIII CATG 5 cut(s) 220, 818, 1121, 1202, 1208
NlaIV GGNNCC 4 cut(s) 103, 367, 570, 862
NmeAIII GCCGAG 1 cut(s) 1156
NmuCI GTSAC 1 cut(s) 416
NspI RCATGY 1 cut(s) 1208
NspV TTCGAA 2 cut(s) 964, 1104
PagI TCATGA 1 cut(s) 1117
PciI ACATGT 1 cut(s) 1204
PdmI GAANNNNTTC 1 cut(s) 781
PfeI GAWTC 2 cut(s) 475, 1024
PkrI GCNGC 4 cut(s) 30, 259, 568, 658
PleI GAGTC 2 cut(s) 130, 153
PpsI GAGTC 2 cut(s) 130, 153
PpuMI RGGWCCY 1 cut(s) 365
PscI ACATGT 1 cut(s) 1204
Psp5II RGGWCCY 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 97, 1076
PspGI CCWGG 2 cut(s) 97, 1076
PspN4I GGNNCC 4 cut(s) 103, 367, 570, 862
PspPI GGNCC 2 cut(s) 365, 1030
PspPPI RGGWCCY 1 cut(s) 365
PstI CTGCAG 1 cut(s) 1168
PstNI CAGNNNCTG 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 1203
SaqAI TTAA 5 cut(s) 597, 708, 848, 933, 1083
SatI GCNGC 4 cut(s) 29, 258, 567, 657
Sau3AI GATC 2 cut(s) 345, 873
Sau96I GGNCC 2 cut(s) 365, 1030
SchI GAGTC 2 cut(s) 131, 153
ScrFI CCNGG 2 cut(s) 99, 1078
SfaNI GCATC 4 cut(s) 126, 520, 868, 925
SfcI CTRYAG 1 cut(s) 1164
SfuI TTCGAA 2 cut(s) 964, 1104
SinI GGWCC 2 cut(s) 365, 1030
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 6 cut(s) 705, 841, 966, 1084, 1100, 1154
SsiI CCGC 4 cut(s) 75, 566, 855, 926
SspMI CTAG 5 cut(s) 12, 339, 359, 498, 773
StyD4I CCNGG 2 cut(s) 97, 1076
StyI CCWWGG 1 cut(s) 1198
TaaI ACNGT 3 cut(s) 58, 245, 1034
TaqI TCGA 7 cut(s) 174, 504, 788, 876, 909, 964, 1104
TasI AATT 6 cut(s) 705, 841, 966, 1084, 1100, 1154
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 2 cut(s) 475, 1024
Tru1I TTAA 5 cut(s) 597, 708, 848, 933, 1083
Tru9I TTAA 5 cut(s) 597, 708, 848, 933, 1083
TscAI CASTG 2 cut(s) 61, 987
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 3 cut(s) 28, 257, 656
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 2 cut(s) 623, 774
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 2 cut(s) 61, 987
VpaK11BI GGWCC 2 cut(s) 365, 1030
XapI RAATTY 2 cut(s) 966, 1100
XceI RCATGY 1 cut(s) 1208
XmiI GTMKAC 1 cut(s) 1036
XmnI GAANNNNTTC 1 cut(s) 781
XspI CTAG 5 cut(s) 12, 339, 359, 498, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.