Rroxscaffold_7G00160980

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000265
Physical Location & Seq
Reverse (-)
3845308 .. 3849840
4533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160980.1

Sequence Viewer

Length: 1296 bp
ATGGCTCCATATCCAAAAGATGAGCATCCTGGTCCTGCATCTAGCTCAAAGTTCAAACTGATCGTAGCTATGGAAAGAACTGGTAGTATCTCTTTACAGCCCCCAACCTTTGGAAACCTAATCACTGTTCTGAGCATCGACGGCGGTGGAATAAGAGGGCTTATCCCAGGAACCATCCTTGCTTTCCTTGAGTCTGAACTTCAGAAGCTGGACGGTGAAGATGCAAGACTCGCCGACTATTTCGATGTGATTTCAGGGACAAGCACAGGTGGTCTCGTAACTGCCATGCTCACAGCCCCAAATGAGAATGACCGCCCACTATTTGCTGCCAAAGATATCAAGGACTTCTACTTGACCCACTCCCCCAAAATCTTCCCCCAGAAGACTGGTTGGCTGTTTCCTCGTGCTAGAAAGATCATCAGAGCTCTAGCAGGACCGAAATACGATGGGAAGTATCTACATAGCTTGGTTAGGGAAAAGCTTGGTGACAAAAAACTGAACCAAACATTGACTAATGTTGTCATTCCTGCATTTGACATAAAGAATCTCCAGCCAGCTATATTTTCCAGCTTCAAGGTGAAAAATAAGCAGCCTTCCTTTGATGCCCTACTTTCGGACATATGTATTGCAACCTCAGCAGCGCCAACTTACCTCCCAGCTCATTATTTTAAAACCAACGGCCTCAAAGGAAAAGTTAGAGAATTTCACCTGATAGATGGTGGATTGGCAGCAAATAATCCGACTTTGATTGCTATTGGTGAAGTGACAAGGGAATTAATTAAGGGGAGTTCAGACTTCTTTCCTATAAAACCAATGGACTATGGAAGATTTCTGGTGATATCCTTAGGAACTGGCTCATCGAAAGCTGAACTGAAATACAATGCTCATGTTGCCTCCAAGTGGGGTGTGTTGAATTGGTTAACGAGCGGTGGTTCCACCCCATTAATCAATGCTTTCAGTCAAGCAAGTGCCGATATGGTCGATTTGCATCTTTCTGTGGTTTTCAAAGCCCTTCACTCGGAGAAAAACTATCTTCGAATTCAGGATGACACGCTAAGTGGCGAAGTATCTTCCGTAGATATAGCAACACAGAAGAATCTGGACCATCTTCTCAGAGTTGGTGAAGGACTATTGAACCAACCAGTTTCCAGGGTTAATTTAGAGACAGGCAAATTCGAAGCTTGTAATCATGAGACTAATAAAGAGGCTCTTATAAGGTTTGCAAAATTGCTGTCCGAAGAGAAGTGGCTTCGTCTTGCAAGGTCTCCCCATGGACATATTGCAAAGTCTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

431

Amino Acids

47.2

Weight (kDa)

9.18

Isoelectric Point (pI)

27.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 44 - 252 4.9e-27 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1214
AccB7I CCANNNNNTGG 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 927
AciI CCGC 3 cut(s) 144, 313, 927
AcsI RAATTY 3 cut(s) 701, 1038, 1172
AcuI CTGAAG 1 cut(s) 185
AfiI CCNNNNNNNGG 4 cut(s) 110, 613, 900, 1018
AgsI TTSAA 5 cut(s) 55, 574, 913, 1006, 1135
AjnI CCWGG 3 cut(s) 28, 166, 1148
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 4 cut(s) 278, 1157, 1187, 1269
AlwNI CAGNNNCTG 1 cut(s) 208
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 326, 589, 638, 728
ApoI RAATTY 3 cut(s) 701, 1038, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 1217, 1249
AseI ATTAAT 2 cut(s) 776, 944
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 3 cut(s) 32, 434, 1102
AsuHPI GGTGA 7 cut(s) 227, 497, 589, 698, 770, 847, 1133
AsuII TTCGAA 2 cut(s) 1036, 1176
AvaII GGWCC 3 cut(s) 32, 434, 1102
AxyI CCTNAGG 1 cut(s) 844
BanII GRGCYC 1 cut(s) 427
BauI CACGAG 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 427
BbvCI CCTCAGC 1 cut(s) 634
BbvI GCAGC 4 cut(s) 313, 601, 650, 740
BccI CCATC 4 cut(s) 182, 440, 710, 1113
BceAI ACGGC 2 cut(s) 157, 694
BciT130I CCWGG 3 cut(s) 30, 168, 1150
BcoDI GTCTC 4 cut(s) 278, 1157, 1187, 1269
BfaI CTAG 3 cut(s) 42, 408, 428
BfoI RGCGCY 1 cut(s) 644
BisI GCNGC 4 cut(s) 327, 590, 639, 729
BlsI GCNGC 4 cut(s) 328, 591, 640, 730
Bme1390I CCNGG 3 cut(s) 30, 168, 1150
Bme18I GGWCC 3 cut(s) 32, 434, 1102
BmgT120I GGNCC 3 cut(s) 32, 434, 1102
BmiI GGNNCC 3 cut(s) 6, 172, 934
BmrFI CCNGG 3 cut(s) 30, 168, 1150
BmsI GCATC 6 cut(s) 34, 47, 144, 211, 592, 997
BpiI GAAGAC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 634
Bpu14I TTCGAA 2 cut(s) 1036, 1176
BpuEI CTTGAG 1 cut(s) 209
BsaBI GATNNNNATC 2 cut(s) 24, 987
BsaI GGTCTC 2 cut(s) 278, 1269
BsaJI CCNNGG 3 cut(s) 166, 1149, 1270
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 613, 900, 1018
Bse1I ACTGG 4 cut(s) 85, 391, 856, 1142
Bse21I CCTNAGG 1 cut(s) 844
Bse8I GATNNNNATC 2 cut(s) 24, 987
BseBI CCWGG 3 cut(s) 30, 168, 1150
BseDI CCNNGG 3 cut(s) 166, 1149, 1270
BseGI GGATG 3 cut(s) 25, 174, 1051
BseJI GATNNNNATC 2 cut(s) 24, 987
BseLI CCNNNNNNNGG 4 cut(s) 110, 613, 900, 1018
BseMII CTCAG 3 cut(s) 122, 648, 1126
BseNI ACTGG 4 cut(s) 85, 391, 856, 1142
BseXI GCAGC 4 cut(s) 313, 601, 650, 740
BseYI CCCAGC 1 cut(s) 655
BshFI GGCC 1 cut(s) 681
BsiHKAI GWGCWC 1 cut(s) 427
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 4 cut(s) 110, 613, 900, 1018
BsmAI GTCTC 4 cut(s) 278, 1157, 1187, 1269
BsmFI GGGAC 1 cut(s) 271
BsnI GGCC 1 cut(s) 681
Bso31I GGTCTC 2 cut(s) 278, 1269
Bsp119I TTCGAA 2 cut(s) 1036, 1176
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 2 cut(s) 60, 414
Bsp19I CCATGG 1 cut(s) 1270
BspACI CCGC 3 cut(s) 144, 313, 927
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 3 cut(s) 123, 647, 1125
BspHI TCATGA 1 cut(s) 1189
BspLI GGNNCC 3 cut(s) 6, 172, 934
BspT104I TTCGAA 2 cut(s) 1036, 1176
BspTNI GGTCTC 2 cut(s) 278, 1269
BsrBI CCGCTC 1 cut(s) 927
BsrI ACTGG 4 cut(s) 85, 391, 856, 1142
BssECI CCNNGG 3 cut(s) 166, 1149, 1270
BssMI GATC 2 cut(s) 60, 414
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 1270
Bst2BI CACGAG 1 cut(s) 402
Bst2UI CCWGG 3 cut(s) 30, 168, 1150
Bst4CI ACNGT 2 cut(s) 127, 215
Bst6I CTCTTC 1 cut(s) 1233
BstBI TTCGAA 2 cut(s) 1036, 1176
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 5 cut(s) 131, 634, 844, 1055, 1112
BstDSI CCRYGG 1 cut(s) 1270
BstF5I GGATG 3 cut(s) 25, 174, 1051
BstH2I RGCGCY 1 cut(s) 644
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 2 cut(s) 63, 417
BstMAI GTCTC 4 cut(s) 278, 1157, 1187, 1269
BstMBI GATC 2 cut(s) 60, 414
BstMWI GCNNNNNNNGC 4 cut(s) 141, 230, 635, 890
BstNI CCWGG 3 cut(s) 30, 168, 1150
BstSCI CCNGG 3 cut(s) 28, 166, 1148
BstV1I GCAGC 4 cut(s) 313, 601, 650, 740
BstV2I GAAGAC 1 cut(s) 389
BstXI CCANNNNNNTGG 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 844
BsuRI GGCC 1 cut(s) 681
BtgI CCRYGG 1 cut(s) 1270
BtsCI GGATG 3 cut(s) 25, 174, 1051
BtsIMutI CAGTG 2 cut(s) 123, 1291
Cac8I GCNNGC 1 cut(s) 555
CaiI CAGNNNCTG 1 cut(s) 208
CciI TCATGA 1 cut(s) 1189
CfoI GCGC 1 cut(s) 643
Cfr13I GGNCC 3 cut(s) 32, 434, 1102
CviAII CATG 4 cut(s) 286, 887, 1190, 1271
DdeI CTNAG 5 cut(s) 131, 634, 844, 1055, 1112
DpnI GATC 2 cut(s) 62, 416
DpnII GATC 2 cut(s) 60, 414
DraI TTTAAA 1 cut(s) 670
Eam1104I CTCTTC 1 cut(s) 1233
EarI CTCTTC 1 cut(s) 1233
Ecl136II GAGCTC 1 cut(s) 425
Eco130I CCWWGG 1 cut(s) 1270
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 2 cut(s) 278, 1269
Eco32I GATATC 2 cut(s) 337, 840
Eco47I GGWCC 3 cut(s) 32, 434, 1102
Eco53kI GAGCTC 1 cut(s) 425
Eco57I CTGAAG 1 cut(s) 185
Eco81I CCTNAGG 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 1038
EcoRII CCWGG 3 cut(s) 28, 166, 1148
EcoRV GATATC 2 cut(s) 337, 840
EcoT14I CCWWGG 1 cut(s) 1270
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 1270
FaeI CATG 4 cut(s) 289, 890, 1193, 1274
FalI AAGNNNNNCTT 2 cut(s) 1194, 1226
FaqI GGGAC 1 cut(s) 271
FatI CATG 4 cut(s) 285, 886, 1189, 1270
FauNDI CATATG 1 cut(s) 620
Fnu4HI GCNGC 4 cut(s) 327, 590, 639, 729
FokI GGATG 3 cut(s) 12, 161, 1058
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 4 cut(s) 327, 590, 639, 729
FspBI CTAG 3 cut(s) 42, 408, 428
GlaI GCGC 1 cut(s) 642
GluI GCNGC 4 cut(s) 327, 590, 639, 729
GsaI CCCAGC 1 cut(s) 659
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 644
HaeIII GGCC 1 cut(s) 681
HhaI GCGC 1 cut(s) 643
Hin1II CATG 4 cut(s) 289, 890, 1193, 1274
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HindIII AAGCTT 2 cut(s) 479, 1179
HinfI GANTC 4 cut(s) 191, 228, 544, 1096
HpaI GTTAAC 1 cut(s) 921
HphI GGTGA 7 cut(s) 227, 497, 589, 698, 770, 847, 1133
Hpy166II GTNNAC 1 cut(s) 921
Hpy188III TCNNGA 3 cut(s) 1043, 1100, 1190
Hpy8I GTNNAC 1 cut(s) 921
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 3 cut(s) 603, 1022, 1118
HpyCH4III ACNGT 2 cut(s) 127, 215
HpyCH4V TGCA 8 cut(s) 38, 224, 530, 629, 988, 1223, 1259, 1283
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 230, 635, 890
HpyF3I CTNAG 5 cut(s) 131, 634, 844, 1055, 1112
Hsp92II CATG 4 cut(s) 289, 890, 1193, 1274
HspAI GCGC 1 cut(s) 641
KspAI GTTAAC 1 cut(s) 921
Kzo9I GATC 2 cut(s) 60, 414
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 313, 601, 650, 740
LweI GCATC 6 cut(s) 34, 47, 144, 211, 592, 997
MaeI CTAG 3 cut(s) 42, 408, 428
MaeIII GTNAC 3 cut(s) 277, 485, 763
MalI GATC 2 cut(s) 62, 416
MbiI CCGCTC 1 cut(s) 927
MboI GATC 2 cut(s) 60, 414
MboII GAAGA 9 cut(s) 230, 364, 394, 837, 1025, 1062, 1100, 1105, 1250
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 8 cut(s) 701, 773, 777, 913, 1038, 1156, 1172, 1226
MlyI GAGTC 2 cut(s) 200, 222
MmeI TCCRAC 1 cut(s) 764
MnlI CCTC 7 cut(s) 149, 411, 643, 662, 692, 904, 1198
MseI TTAA 6 cut(s) 669, 776, 780, 920, 944, 1155
MspR9I CCNGG 3 cut(s) 30, 168, 1150
MvaI CCWGG 3 cut(s) 30, 168, 1150
MwoI GCNNNNNNNGC 4 cut(s) 141, 230, 635, 890
NcoI CCATGG 1 cut(s) 1270
NdeI CATATG 1 cut(s) 620
NdeII GATC 2 cut(s) 60, 414
NlaIII CATG 4 cut(s) 289, 890, 1193, 1274
NlaIV GGNNCC 3 cut(s) 6, 172, 934
NmuCI GTSAC 2 cut(s) 485, 763
NspV TTCGAA 2 cut(s) 1036, 1176
PacI TTAATTAA 1 cut(s) 780
PagI TCATGA 1 cut(s) 1189
PfeI GAWTC 2 cut(s) 544, 1096
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 4 cut(s) 328, 591, 640, 730
PleI GAGTC 2 cut(s) 199, 222
PpsI GAGTC 2 cut(s) 199, 222
PshBI ATTAAT 2 cut(s) 776, 944
PsiI TTATAA 1 cut(s) 1214
Psp124BI GAGCTC 1 cut(s) 427
Psp6I CCWGG 3 cut(s) 28, 166, 1148
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 3 cut(s) 28, 166, 1148
PspN4I GGNNCC 3 cut(s) 6, 172, 934
PspPI GGNCC 3 cut(s) 32, 434, 1102
PstNI CAGNNNCTG 1 cut(s) 208
SacI GAGCTC 1 cut(s) 427
SaqAI TTAA 6 cut(s) 669, 776, 780, 920, 944, 1155
SatI GCNGC 4 cut(s) 327, 590, 639, 729
Sau3AI GATC 2 cut(s) 60, 414
Sau96I GGNCC 3 cut(s) 32, 434, 1102
SchI GAGTC 2 cut(s) 200, 222
ScrFI CCNGG 3 cut(s) 30, 168, 1150
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 6 cut(s) 34, 47, 144, 211, 592, 997
SfuI TTCGAA 2 cut(s) 1036, 1176
SinI GGWCC 3 cut(s) 32, 434, 1102
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 8 cut(s) 701, 773, 777, 913, 1038, 1156, 1172, 1226
SsiI CCGC 3 cut(s) 144, 313, 927
SspMI CTAG 3 cut(s) 42, 408, 428
SstI GAGCTC 1 cut(s) 427
StyD4I CCNGG 3 cut(s) 28, 166, 1148
StyI CCWWGG 1 cut(s) 1270
TaaI ACNGT 2 cut(s) 127, 215
TaqI TCGA 6 cut(s) 138, 243, 860, 981, 1036, 1176
TaqII GACCGA 1 cut(s) 451
TasI AATT 8 cut(s) 701, 773, 777, 913, 1038, 1156, 1172, 1226
TfiI GAWTC 2 cut(s) 544, 1096
Tru1I TTAA 6 cut(s) 669, 776, 780, 920, 944, 1155
Tru9I TTAA 6 cut(s) 669, 776, 780, 920, 944, 1155
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 2 cut(s) 485, 763
TseI GCWGC 4 cut(s) 326, 589, 638, 728
Tsp45I GTSAC 2 cut(s) 485, 763
TspGWI ACGGA 1 cut(s) 1063
TspRI CASTG 1 cut(s) 130
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 32, 434, 1102
VspI ATTAAT 2 cut(s) 776, 944
XapI RAATTY 3 cut(s) 701, 1038, 1172
XspI CTAG 3 cut(s) 42, 408, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.