Rh6BG431900

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000265
Physical Location & Seq
Reverse (-)
65165762 .. 65168623
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG431900.1

Sequence Viewer

Length: 1236 bp
ATGGAAAGAACCAAAAGCATCCCACTACAGCCACCAACTTATGGAAACCTAATCACTGTTCTAAGCATCGATGGAGGTGGAATAAGAGGGCTTATCCCAGGAACAATCCTTGCTTTTCTTGAATCTGAGCTTCAGAAGCTGGATGGAGAAGATGTGAGACTCGCCGACTACTTTGATGTGATTTCAGGGACAAGCACAGGAGGTCTTGTGACTGCCATGCTTACAACCCCAGATGAGAATAACAGACCGATGTTTGCTGCTAAAGATATAAAGGACTTCTACCTTACTCATAGCCCCAAAATCTTCCCCCAGAAGACATGGTGGCTGTTTTCTAGTCTTATAAACATCATCAAATCTCTAGTAGGACCAAAACACGATGGGAAGTATCTACATAGCTTGATTAGGGAAAAACTTGGTGACAAGAAACTGCATCAGACATTGACTAATGTTGTCATTCCTGCGTTTGATATAAAGAATCTCCAGCCAACAATCTTTTCCAGCTTTGAGGTGAAAAAGAAGCCATCCTTTGATGCTTTGCTTTCAGACATAAGCATCGCAACCTCAGCAGCACCAACTTATCTTCCAGCTTATTATTTTGAAACCAAGGAAGCGGAAGGGAAAGTAAGAGAATTCAACCTTATAGATGGTGGGGTAGCTGCAAATAATCCGACTCTAGTTGCTATTGGTGAAGTTACAAAGGAAATAATCAAGGGGAGCTCCGACTTTTTCCCTATAAAACCTATGGACTATGGAAGGTTTTTGGTTATATCATTAGGAACTGGCTCACCAAAAATTGAATTGAAGTACAATGCGCCGAGCGCAGCTAAATGGGGTATCTTGAATTGGTTAACCAGCGGTGGTTCCACTCCAATCATTAACGTTTTTAGTCATGCAAGTGCCGATATGGTCGATCTTCACCTTTCTGTAGTTTTCCAAGCCCTTCACTCAGAGAAAAATTATCTTCGAATTCAGGATGACACGCTAAGTGGAAAAGTATCTTCGGTGGATGTAGCCACGAAGAAAAATTTGAACGATATCCTAGGAGTTGGAGAAGGCCTATTGAAGAAACCAGTGTCTAGGGTTAATTTGGAGAATGGCAGATTTGAGGCTTCCAATCATGAGACCAACGAAGAGGCTCTTATAAGGTTTGCAAAACTACTCTCTGAAGAGAAGTGGCTTCGCCTTGCAAGGTCGCCCCACGGAAATTGTGACATGCCCATGCCGAACTCCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

411

Amino Acids

45.27

Weight (kDa)

6.81

Isoelectric Point (pI)

28.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 21 - 228 2.1e-25 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 341, 1142
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 2 cut(s) 611, 855
AclI AACGTT 1 cut(s) 879
AcsI RAATTY 3 cut(s) 629, 966, 1024
AcuI CTGAAG 2 cut(s) 116, 1185
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 8 cut(s) 122, 599, 634, 797, 802, 841, 1030, 1063
AjnI CCWGG 1 cut(s) 97
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 8 cut(s) 130, 139, 396, 501, 587, 656, 717, 824
AluI AGCT 8 cut(s) 130, 139, 396, 501, 587, 656, 717, 824
Alw21I GWGCWC 1 cut(s) 719
Alw26I GTCTC 2 cut(s) 151, 1115
AlwNI CAGNNNCTG 1 cut(s) 139
AoxI GGCC 1 cut(s) 1054
ApeKI GCWGC 4 cut(s) 257, 566, 656, 821
ApoI RAATTY 3 cut(s) 629, 966, 1024
AspA2I CCTAGG 1 cut(s) 1039
AspLEI GCGC 2 cut(s) 814, 821
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 5 cut(s) 428, 520, 698, 777, 908
AsuII TTCGAA 1 cut(s) 964
AvaII GGWCC 1 cut(s) 365
AvrII CCTAGG 1 cut(s) 1039
BanII GRGCYC 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 719
BbvCI CCTCAGC 1 cut(s) 562
BbvI GCAGC 4 cut(s) 244, 578, 643, 833
BccI CCATC 5 cut(s) 65, 137, 371, 529, 638
BciT130I CCWGG 1 cut(s) 99
BcoDI GTCTC 2 cut(s) 151, 1115
BfaI CTAG 5 cut(s) 333, 359, 674, 1040, 1077
BfmI CTRYAG 2 cut(s) 26, 924
BisI GCNGC 4 cut(s) 258, 567, 657, 822
BlnI CCTAGG 1 cut(s) 1039
BlsI GCNGC 4 cut(s) 259, 568, 658, 823
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 365
BmgT120I GGNCC 1 cut(s) 365
BmiI GGNNCC 1 cut(s) 862
BmrFI CCNGG 1 cut(s) 99
BmsI GCATC 5 cut(s) 27, 75, 439, 520, 561
BpiI GAAGAC 1 cut(s) 320
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 1 cut(s) 562
Bpu14I TTCGAA 1 cut(s) 964
Bsa29I ATCGAT 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 1115
BsaJI CCNNGG 4 cut(s) 97, 603, 1039, 1198
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 192, 222
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 2 cut(s) 784, 1070
BseBI CCWGG 1 cut(s) 99
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 4 cut(s) 97, 603, 1039, 1198
BseGI GGATG 5 cut(s) 18, 148, 521, 979, 1012
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMII CTCAG 3 cut(s) 117, 576, 960
BseNI ACTGG 2 cut(s) 784, 1070
BseXI GCAGC 4 cut(s) 244, 578, 643, 833
BshFI GGCC 1 cut(s) 1056
BshVI ATCGAT 1 cut(s) 69
BsiHKAI GWGCWC 1 cut(s) 719
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 41
BsmAI GTCTC 2 cut(s) 151, 1115
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 1 cut(s) 1056
Bso31I GGTCTC 1 cut(s) 1115
Bsp119I TTCGAA 1 cut(s) 964
Bsp1286I GDGCHC 1 cut(s) 719
Bsp143I GATC 1 cut(s) 910
BspACI CCGC 2 cut(s) 611, 855
BspANI GGCC 1 cut(s) 1056
BspCNI CTCAG 3 cut(s) 118, 575, 959
BspDI ATCGAT 1 cut(s) 69
BspHI TCATGA 1 cut(s) 1117
BspLI GGNNCC 1 cut(s) 862
BspT104I TTCGAA 1 cut(s) 964
BspTNI GGTCTC 1 cut(s) 1115
BsrI ACTGG 2 cut(s) 784, 1070
BssECI CCNNGG 4 cut(s) 97, 603, 1039, 1198
BssMI GATC 1 cut(s) 910
BssT1I CCWWGG 2 cut(s) 603, 1039
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 1125, 1161
BstBI TTCGAA 1 cut(s) 964
BstDEI CTNAG 5 cut(s) 62, 126, 562, 946, 983
BstDSI CCRYGG 1 cut(s) 1198
BstF5I GGATG 5 cut(s) 18, 148, 521, 979, 1012
BstHHI GCGC 2 cut(s) 814, 821
BstKTI GATC 1 cut(s) 913
BstMAI GTCTC 2 cut(s) 151, 1115
BstMBI GATC 1 cut(s) 910
BstMWI GCNNNNNNNGC 3 cut(s) 136, 563, 818
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 1 cut(s) 1216
BstSCI CCNGG 1 cut(s) 97
BstSFI CTRYAG 2 cut(s) 26, 924
BstV1I GCAGC 4 cut(s) 244, 578, 643, 833
BstV2I GAAGAC 1 cut(s) 320
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 1 cut(s) 1056
BsuTUI ATCGAT 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 1198
BtgZI GCGATG 1 cut(s) 538
BtsCI GGATG 5 cut(s) 18, 148, 521, 979, 1012
BtsIMutI CAGTG 2 cut(s) 54, 1077
CaiI CAGNNNCTG 1 cut(s) 139
CciI TCATGA 1 cut(s) 1117
CfoI GCGC 2 cut(s) 814, 821
Cfr13I GGNCC 1 cut(s) 365
ClaI ATCGAT 1 cut(s) 69
Csp6I GTAC 1 cut(s) 805
CspCI CAANNNNNGTGG 2 cut(s) 1187, 1222
CviAII CATG 6 cut(s) 217, 318, 890, 1118, 1213, 1219
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 5 cut(s) 62, 126, 562, 946, 983
DpnI GATC 1 cut(s) 912
DpnII GATC 1 cut(s) 910
Eam1104I CTCTTC 2 cut(s) 1125, 1161
EarI CTCTTC 2 cut(s) 1125, 1161
Ecl136II GAGCTC 1 cut(s) 717
Eco130I CCWWGG 2 cut(s) 603, 1039
Eco147I AGGCCT 1 cut(s) 1056
Eco24I GRGCYC 1 cut(s) 719
Eco31I GGTCTC 1 cut(s) 1115
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 717
Eco57I CTGAAG 2 cut(s) 116, 1185
EcoICRI GAGCTC 1 cut(s) 717
EcoRI GAATTC 2 cut(s) 629, 966
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 2 cut(s) 603, 1039
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 2 cut(s) 603, 1039
FaeI CATG 6 cut(s) 220, 321, 893, 1121, 1216, 1222
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 1122, 1154
FaqI GGGAC 1 cut(s) 202
FatI CATG 6 cut(s) 216, 317, 889, 1117, 1212, 1218
Fnu4HI GCNGC 4 cut(s) 258, 567, 657, 822
FokI GGATG 5 cut(s) 5, 155, 508, 986, 1019
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 4 cut(s) 258, 567, 657, 822
FspBI CTAG 5 cut(s) 333, 359, 674, 1040, 1077
GlaI GCGC 2 cut(s) 813, 820
GluI GCNGC 4 cut(s) 258, 567, 657, 822
GsuI CTGGAG 1 cut(s) 464
HaeIII GGCC 1 cut(s) 1056
HhaI GCGC 2 cut(s) 814, 821
Hin1II CATG 6 cut(s) 220, 321, 893, 1121, 1216, 1222
Hin6I GCGC 2 cut(s) 812, 819
HinP1I GCGC 2 cut(s) 812, 819
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HinfI GANTC 4 cut(s) 122, 159, 475, 670
HpaI GTTAAC 1 cut(s) 849
HphI GGTGA 5 cut(s) 428, 520, 698, 777, 908
Hpy166II GTNNAC 1 cut(s) 849
Hpy188I TCNGA 8 cut(s) 127, 135, 435, 544, 669, 721, 949, 1165
Hpy188III TCNNGA 4 cut(s) 119, 838, 971, 1118
Hpy8I GTNNAC 1 cut(s) 849
HpyAV CCTTC 4 cut(s) 608, 747, 950, 1046
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 5 cut(s) 430, 659, 893, 1151, 1187
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 563, 818
HpyF3I CTNAG 5 cut(s) 62, 126, 562, 946, 983
HpySE526I ACGT 1 cut(s) 879
Hsp92II CATG 6 cut(s) 220, 321, 893, 1121, 1216, 1222
HspAI GCGC 2 cut(s) 812, 819
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 1 cut(s) 910
LmnI GCTCC 2 cut(s) 714, 722
Lsp1109I GCAGC 4 cut(s) 244, 578, 643, 833
LweI GCATC 5 cut(s) 27, 75, 439, 520, 561
MaeI CTAG 5 cut(s) 333, 359, 674, 1040, 1077
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 4 cut(s) 208, 416, 691, 1208
MalI GATC 1 cut(s) 912
MboI GATC 1 cut(s) 910
MhlI GDGCHC 1 cut(s) 719
MluCI AATT 9 cut(s) 629, 792, 797, 841, 955, 966, 1024, 1084, 1204
MlyI GAGTC 2 cut(s) 153, 664
MmeI TCCRAC 3 cut(s) 692, 744, 1027
MnlI CCTC 7 cut(s) 68, 80, 194, 499, 571, 1099, 1126
MseI TTAA 3 cut(s) 848, 876, 1083
MslI CAYNNNNRTG 2 cut(s) 894, 1217
MspA1I CMGCKG 1 cut(s) 855
MspR9I CCNGG 1 cut(s) 99
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 136, 563, 818
NdeII GATC 1 cut(s) 910
NlaIII CATG 6 cut(s) 220, 321, 893, 1121, 1216, 1222
NlaIV GGNNCC 1 cut(s) 862
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 3 cut(s) 208, 416, 1208
NspI RCATGY 1 cut(s) 1216
NspV TTCGAA 1 cut(s) 964
PagI TCATGA 1 cut(s) 1117
PceI AGGCCT 1 cut(s) 1056
PfeI GAWTC 2 cut(s) 122, 475
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 4 cut(s) 259, 568, 658, 823
PleI GAGTC 2 cut(s) 153, 664
PpsI GAGTC 2 cut(s) 153, 664
PsiI TTATAA 2 cut(s) 341, 1142
Psp124BI GAGCTC 1 cut(s) 719
Psp1406I AACGTT 1 cut(s) 879
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 862
PspPI GGNCC 1 cut(s) 365
PstNI CAGNNNCTG 1 cut(s) 139
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
RseI CAYNNNNRTG 2 cut(s) 894, 1217
SacI GAGCTC 1 cut(s) 719
SaqAI TTAA 3 cut(s) 848, 876, 1083
SatI GCNGC 4 cut(s) 258, 567, 657, 822
Sau3AI GATC 1 cut(s) 910
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 2 cut(s) 153, 664
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 1 cut(s) 719
SfaNI GCATC 5 cut(s) 27, 75, 439, 520, 561
SfcI CTRYAG 2 cut(s) 26, 924
SfuI TTCGAA 1 cut(s) 964
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 894, 1217
Sse9I AATT 9 cut(s) 629, 792, 797, 841, 955, 966, 1024, 1084, 1204
SseBI AGGCCT 1 cut(s) 1056
SsiI CCGC 2 cut(s) 611, 855
SspMI CTAG 5 cut(s) 333, 359, 674, 1040, 1077
SstI GAGCTC 1 cut(s) 719
StuI AGGCCT 1 cut(s) 1056
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 2 cut(s) 603, 1039
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 882
TaqI TCGA 3 cut(s) 69, 909, 964
TaqII GACCGA 1 cut(s) 262
TasI AATT 9 cut(s) 629, 792, 797, 841, 955, 966, 1024, 1084, 1204
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 2 cut(s) 122, 475
Tru1I TTAA 3 cut(s) 848, 876, 1083
Tru9I TTAA 3 cut(s) 848, 876, 1083
TscAI CASTG 2 cut(s) 61, 1077
TseFI GTSAC 3 cut(s) 208, 416, 1208
TseI GCWGC 4 cut(s) 257, 566, 656, 821
Tsp45I GTSAC 3 cut(s) 208, 416, 1208
TspGWI ACGGA 1 cut(s) 1215
TspRI CASTG 2 cut(s) 61, 1077
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 3 cut(s) 629, 966, 1024
XceI RCATGY 1 cut(s) 1216
XmaJI CCTAGG 1 cut(s) 1039
XspI CTAG 5 cut(s) 333, 359, 674, 1040, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.