pycom15g08140

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000265
Physical Location & Seq
Reverse (-)
5048634 .. 5049752
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08140.1

Sequence Viewer

Length: 747 bp
ATGGGCATTTATGTGGTTTTTTACCAGGTGGAAAAGAAGCCGTGCACCGATGCCTTACTCTCCGACATATGCATTGCAACCTCAGCTGCACCAACTTACCTTCCAGCTCATTATTTTGAAACCAAGTATGCTGCAGGAACAGTTAGAGAATATAACCTTATAGATGGCGGCGTGGCTGCAAATAATCCGACATTTGTTGCTATTTGTGAAGTGGTAAACGAAATAGAGAAGGAGAATTCAGATATCGTTTCTGTAAAACGTGTGGACTATGGACGATTTCTAGTAATATCATTAGGAACTGGCTCATCAAAAGCTGAACGGGAATACCATGCATCTGCTGCAGCCAAATGGGGCCTCTTGAATTGGTTAACCAGCGGTGGTTCCACCCCAATCATTGACGTTTTCAGTCAAGCAAGCGCCGATATGGTTGATCTTCACCTTTCTCGCGTTTTTCAAGCCTTTAATTCTGAAGAAAACTATCTTCGAATTCAGGACGATACCCTTCACGGGACAGTATCTTCTGTAGATGTAGCAACAAAGAAAAATTTGGCCAATCTTGTGAAAGTTGGTGAAGGGTTATTGAAACAACCAGTTTCCAGGGTTAATTTGGACAGTGGCAGGTTTGAGGCTTCTAAGCATGAGACTAATGCAGAGGCTCTTACAAGGTTTGCTAAACTACTCTCCGAAGAGAAGCTGTTTCGCCAAGCAAGCAGGTCAACTAATGAGCCTGCTGGAAATACCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

249

Amino Acids

27.17

Weight (kDa)

5.6

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 9 - 68 1.1e-07 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26560
fragaria_vesca FvH4_2g34690 FvH4_2g34750 FvH4_2g34750 FvH4_2g34754 FvH4_2g34770 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34780 FvH4_2g34800 FvH4_2g34800 FvH4_2g34811 FvH4_4g24280 FvH4_4g24280
malus_domestica MD08G1104900.v1.1 MD08G1105100.v1.1 MD08G1105300.v1.1 MD08G1105500.v1.1 MD08G1105900.v1.1 MD15G1085500.v1.1 MD15G1085600.v1.1 MD15G1085800.v1.1 MD15G1085900.v1.1 MD15G1086000.v1.1 MD15G1086500.v1.1
prunus_persica Prupe.1G439600_v2.0.a1 Prupe.1G439700_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439800_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G439900_v2.0.a1 Prupe.1G440100_v2.0.a1
pyrus_communis pycom08g08620 pycom08g08640 pycom08g08650 pycom08g08680 pycom08g08700 pycom15g08080 pycom15g08090 pycom15g08110 pycom15g08140
rosa_chinensis RchiOBHm_Chr3g0482851 RchiOBHm_Chr3g0482871 RchiOBHm_Chr6g0307241 RchiOBHm_Chr6g0307291 RchiOBHm_Chr6g0307341 RchiOBHm_Chr6g0307351 RchiOBHm_Chr6g0307381 RchiOBHm_Chr6g0307391 RchiOBHm_Chr6g0307491 RchiOBHm_Chr6g0307661
rosa_laevigata RLG00000008291 RLG00000010711 RLG00000010718 RLG00000010719 RLG00000010721 RLG00000010722 RLG00000023319 RLG00000023320
rosa_multiflora Rmu_co8005452.1_g000001 Rmu_co8126120.1_g000001 Rmu_sc0000031.1_g000008 Rmu_sc0000031.1_g000009 Rmu_sc0000031.1_g000011 Rmu_sc0000031.1_g000020 Rmu_sc0001612.1_g000003 Rmu_sc0003419.1_g000004 Rmu_sc0005715.1_g000002 Rmu_sc0005715.1_g000003 Rmu_sc0010071.1_g000001 Rmu_sc0010071.1_g000008 Rmu_ssc0000175.1_g000026 Rmu_ssc0000175.1_g000028
rosa_roxburghii Rroxscaffold_2G00138670 Rroxscaffold_6G00398870 Rroxscaffold_7G00160950 Rroxscaffold_7G00160980 Rroxscaffold_7G00161030 Rroxscaffold_7G00161040 Rroxscaffold_7G00161060
rosa_rugosa Rorug03G0201600 Rorug03G0201900 Rorug05G0124400 Rorug06G0357900 Rorug06G0358000 Rorug06G0358200 Rorug06G0358300 Rorug06G0358400 Rorug06G0358700 Rorug06G0358800
rosa_samantha Rh2AG174100 Rh2AG174200 Rh2AG174600 Rh2BG015200 Rh2CG179000 Rh2CG179100 Rh2CG179200 Rh2CG181300 Rh2DG180400 Rh3AG250400 Rh3AG250600 Rh3BG286800 Rh3CG284300 Rh3DG279900 Rh5DG283200 Rh5DG283300 Rh5DG283400 Rh6AG468500 Rh6AG468700 Rh6BG431300 Rh6BG431600 Rh6BG431700 Rh6BG431800 Rh6BG431900 Rh6BG479000 Rh6BG479300 Rh6CG483700 Rh6CG483800 Rh6CG484700 Rh6DG470300 Rh6DG470400 Rh6DG470600 Rh6DG471300 Rh6DG471500
rosa_wichuraiana Rw3G022650 Rw3G022670 Rw6G040840 Rw6G040850 Rw6G040920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 609, 702
AccII CGCG 1 cut(s) 447
AciI CCGC 2 cut(s) 168, 375
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 3 cut(s) 235, 486, 544
AcuI CTGAAG 1 cut(s) 489
AfiI CCNNNNNNNGG 1 cut(s) 507
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 4 cut(s) 119, 361, 455, 583
AjnI CCWGG 2 cut(s) 24, 596
AluBI AGCT 4 cut(s) 86, 107, 314, 694
AluI AGCT 4 cut(s) 86, 107, 314, 694
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 635
Alw44I GTGCAC 1 cut(s) 43
AoxI GGCC 2 cut(s) 352, 549
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 5 cut(s) 86, 131, 176, 338, 341
ApoI RAATTY 3 cut(s) 235, 486, 544
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 428, 581
AsuII TTCGAA 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 47
BalI TGGCCA 1 cut(s) 551
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 5 cut(s) 73, 118, 163, 325, 353
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 25
BciT130I CCWGG 2 cut(s) 26, 598
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 3 cut(s) 132, 339, 522
BfoI RGCGCY 1 cut(s) 420
BfuAI ACCTGC 2 cut(s) 609, 702
BisI GCNGC 6 cut(s) 87, 132, 169, 177, 339, 342
BlsI GCNGC 6 cut(s) 88, 133, 170, 178, 340, 343
Bme1390I CCNGG 2 cut(s) 26, 598
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 353, 382
BmrFI CCNGG 2 cut(s) 26, 598
BmsI GCATC 2 cut(s) 40, 341
Bpu10I CCTNAGC 1 cut(s) 82
Bpu14I TTCGAA 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 597
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 2 cut(s) 304, 590
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 26, 598
BseDI CCNNGG 1 cut(s) 597
BseLI CCNNNNNNNGG 1 cut(s) 507
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 96
BseNI ACTGG 2 cut(s) 304, 590
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 5 cut(s) 73, 118, 163, 325, 353
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 2 cut(s) 354, 551
BsiHKAI GWGCWC 1 cut(s) 47
BslFI GGGAC 1 cut(s) 523
BslI CCNNNNNNNGG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 635
BsmFI GGGAC 1 cut(s) 523
BsnI GGCC 2 cut(s) 354, 551
Bsp119I TTCGAA 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 1 cut(s) 430
BspACI CCGC 2 cut(s) 168, 375
BspANI GGCC 2 cut(s) 354, 551
BspCNI CTCAG 1 cut(s) 95
BspFNI CGCG 1 cut(s) 447
BspLI GGNNCC 2 cut(s) 353, 382
BspMAI CTGCAG 2 cut(s) 136, 343
BspMI ACCTGC 2 cut(s) 609, 702
BspT104I TTCGAA 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 72
BsrI ACTGG 2 cut(s) 304, 590
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 1 cut(s) 430
Bst2UI CCWGG 2 cut(s) 26, 598
Bst4CI ACNGT 3 cut(s) 142, 514, 614
Bst6I CTCTTC 1 cut(s) 681
BstAPI GCANNNNNTGC 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 484
BstC8I GCNNGC 3 cut(s) 415, 709, 729
BstDEI CTNAG 2 cut(s) 82, 633
BstFNI CGCG 1 cut(s) 447
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 1 cut(s) 433
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 1 cut(s) 430
BstMWI GCNNNNNNNGC 3 cut(s) 83, 338, 708
BstNI CCWGG 2 cut(s) 26, 598
BstSCI CCNGG 2 cut(s) 24, 596
BstSFI CTRYAG 3 cut(s) 132, 339, 522
BstSLI GKGCMC 1 cut(s) 47
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 5 cut(s) 73, 118, 163, 325, 353
BsuRI GGCC 2 cut(s) 354, 551
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 2 cut(s) 609, 702
Cac8I GCNNGC 3 cut(s) 415, 709, 729
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 352
CsiI ACCWGGT 1 cut(s) 24
CviAII CATG 2 cut(s) 329, 638
DdeI CTNAG 2 cut(s) 82, 633
DpnI GATC 1 cut(s) 432
DpnII GATC 1 cut(s) 430
EaeI YGGCCR 1 cut(s) 549
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco32I GATATC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 2 cut(s) 235, 486
EcoRII CCWGG 2 cut(s) 24, 596
EcoRV GATATC 1 cut(s) 244
EcoT22I ATGCAT 2 cut(s) 74, 334
FaeI CATG 2 cut(s) 332, 641
FaqI GGGAC 1 cut(s) 523
FatI CATG 2 cut(s) 328, 637
FauNDI CATATG 1 cut(s) 68
Fnu4HI GCNGC 6 cut(s) 87, 132, 169, 177, 339, 342
Fsp4HI GCNGC 6 cut(s) 87, 132, 169, 177, 339, 342
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 418
GluI GCNGC 6 cut(s) 87, 132, 169, 177, 339, 342
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 2 cut(s) 354, 551
HhaI GCGC 1 cut(s) 419
Hin1II CATG 2 cut(s) 332, 641
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HincII GTYRAC 2 cut(s) 369, 717
HindII GTYRAC 2 cut(s) 369, 717
HpaI GTTAAC 1 cut(s) 369
HphI GGTGA 2 cut(s) 428, 581
Hpy166II GTNNAC 5 cut(s) 45, 217, 265, 369, 717
Hpy188I TCNGA 5 cut(s) 64, 189, 241, 469, 685
Hpy188III TCNNGA 2 cut(s) 358, 491
Hpy8I GTNNAC 5 cut(s) 45, 217, 265, 369, 717
HpyAV CCTTC 4 cut(s) 110, 223, 512, 566
HpyCH4III ACNGT 3 cut(s) 142, 514, 614
HpyCH4IV ACGT 2 cut(s) 259, 399
HpyCH4V TGCA 9 cut(s) 45, 72, 77, 89, 134, 179, 332, 341, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 338, 708
HpyF3I CTNAG 2 cut(s) 82, 633
HpySE526I ACGT 2 cut(s) 259, 399
Hsp92II CATG 2 cut(s) 332, 641
HspAI GCGC 1 cut(s) 417
KspAI GTTAAC 1 cut(s) 369
Kzo9I GATC 1 cut(s) 430
Lsp1109I GCAGC 5 cut(s) 73, 118, 163, 325, 353
LweI GCATC 2 cut(s) 40, 341
MabI ACCWGGT 1 cut(s) 24
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 259, 399
MalI GATC 1 cut(s) 432
MboI GATC 1 cut(s) 430
MboII GAAGA 5 cut(s) 425, 473, 482, 510, 698
MhlI GDGCHC 1 cut(s) 47
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 6 cut(s) 235, 361, 463, 486, 544, 604
MluNI TGGCCA 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 87, 212
MnlI CCTC 4 cut(s) 91, 365, 619, 646
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 2 cut(s) 74, 334
MscI TGGCCA 1 cut(s) 551
MseI TTAA 3 cut(s) 368, 462, 603
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 2 cut(s) 86, 375
MspR9I CCNGG 2 cut(s) 26, 598
MvaI CCWGG 2 cut(s) 26, 598
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 3 cut(s) 83, 338, 708
NdeI CATATG 1 cut(s) 68
NdeII GATC 1 cut(s) 430
NlaIII CATG 2 cut(s) 332, 641
NlaIV GGNNCC 2 cut(s) 353, 382
NsiI ATGCAT 2 cut(s) 74, 334
NspV TTCGAA 1 cut(s) 484
PkrI GCNGC 6 cut(s) 88, 133, 170, 178, 340, 343
Psp6I CCWGG 2 cut(s) 24, 596
PspGI CCWGG 2 cut(s) 24, 596
PspN4I GGNNCC 2 cut(s) 353, 382
PspPI GGNCC 1 cut(s) 352
PstI CTGCAG 2 cut(s) 136, 343
PvuII CAGCTG 1 cut(s) 86
RseI CAYNNNNRTG 1 cut(s) 11
SaqAI TTAA 3 cut(s) 368, 462, 603
SatI GCNGC 6 cut(s) 87, 132, 169, 177, 339, 342
Sau3AI GATC 1 cut(s) 430
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 26, 598
SduI GDGCHC 1 cut(s) 47
SexAI ACCWGGT 1 cut(s) 24
SfaNI GCATC 2 cut(s) 40, 341
SfcI CTRYAG 3 cut(s) 132, 339, 522
SfuI TTCGAA 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 6 cut(s) 235, 361, 463, 486, 544, 604
SsiI CCGC 2 cut(s) 168, 375
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 24, 596
TaaI ACNGT 3 cut(s) 142, 514, 614
TaiI ACGT 2 cut(s) 262, 402
TaqI TCGA 1 cut(s) 484
TasI AATT 6 cut(s) 235, 361, 463, 486, 544, 604
TauI GCSGC 1 cut(s) 171
Tru1I TTAA 3 cut(s) 368, 462, 603
Tru9I TTAA 3 cut(s) 368, 462, 603
TscAI CASTG 1 cut(s) 619
TseI GCWGC 5 cut(s) 86, 131, 176, 338, 341
TspRI CASTG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 43
XapI RAATTY 3 cut(s) 235, 486, 544
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XspI CTAG 1 cut(s) 281
Zsp2I ATGCAT 2 cut(s) 74, 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.