FvH4_5g09280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000544
Physical Location & Seq
Forward (+)
5341301 .. 5345784
4484 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09280.t2

Sequence Viewer

Length: 855 bp
ATGACGAAGCGCAAGAGATGTGCCACTCACAACAATGTGAGCAAGATGCTCTGCGTACAAGACGTGATCGAATGGGAGGGCTCAACATTCTATTACGTAAGGAATCGGCGCGGAATTGCTCTATTCAAACAAGCCAAACCCTTATTACAGCAATACGATCGGGACGTATTAACAGCATTGACGCACCGATGGATAAACAATGAAACTTGTCCATCGTTGAGTACTAAAAATGTAGGGATCAGAGACAATAAGGCCTTCCTCCCTTTGGAAGGTCTGAACGGTCGCAGAGATTATCCAACCCTTTATCTGGAGTATCTCATTGATGTTCATGGTACAGACGGTGAATTTAGGGCACGGGATGACCTGTACCAGAAACTGAGGCAGCCTATGTTTCTGAGCGCCTTCAAAAACATATATTTCCACCCTTTCCACCAAGGTGAGGAAGAACTCTCAGACTTTCCTGCCCGTGTTGTGGAGAATTTGAAGTTCAGAAAGGTCCAGTATAGGTGTAAGGGACAGAAATGGATCAAGGCGTATAATCACTGGGAAGGGAGGTTCTATGGAAGGAGGATCGATCTTATTGAAACTGTAATGGTGGACCTTCAGCTTCTAAGAGTGTATGTGAAGGGCAGTTACAAAAATACTGCTGCTAAAGAGGCTGCACTAGTAAGGAATATCATCCTTCACCTCAACGAACAAAAGATTAAGGTTGACACGGAGATCTATGACAAAACAGAGATGTTGGAAATCATTTATGATCACTTCCCGGATATCAACTTCATGCTGTTTGAATTTTGCCAGTATTGTGGAGTCGATTTGGACTTCACAAAACTGCGCAAATTTTATAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

33.89

Weight (kDa)

9.11

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
Acc16I TGCGCA 1 cut(s) 836
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 111
AclWI GGATC 3 cut(s) 245, 533, 578
AcsI RAATTY 4 cut(s) 344, 478, 791, 839
AcuI CTGAAG 1 cut(s) 587
AfaI GTAC 4 cut(s) 57, 223, 334, 368
AfiI CCNNNNNNNGG 5 cut(s) 265, 269, 307, 439, 472
AgsI TTSAA 5 cut(s) 127, 406, 484, 584, 791
AhlI ACTAGT 1 cut(s) 664
AjiI CACGTC 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 435
AloI GAACNNNNNNTCC 2 cut(s) 539, 571
AluBI AGCT 2 cut(s) 607, 851
AluI AGCT 2 cut(s) 607, 851
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 3 cut(s) 245, 533, 578
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 382, 647, 659
ApoI RAATTY 4 cut(s) 344, 478, 791, 839
AspLEI GCGC 4 cut(s) 12, 111, 401, 837
AspS9I GGNCC 2 cut(s) 496, 598
AsuC2I CCSGG 1 cut(s) 767
AsuHPI GGTGA 3 cut(s) 353, 449, 677
AvaII GGWCC 2 cut(s) 496, 598
BaeGI GKGCMC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 83
BarI GAAGNNNNNNTAC 2 cut(s) 617, 649
BbvI GCAGC 3 cut(s) 394, 634, 646
BccI CCATC 2 cut(s) 183, 220
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BclI TGATCA 1 cut(s) 757
BcnI CCSGG 1 cut(s) 767
BcoDI GTCTC 1 cut(s) 237
BcuI ACTAGT 1 cut(s) 664
BfaI CTAG 1 cut(s) 665
BfoI RGCGCY 1 cut(s) 402
BglII AGATCT 1 cut(s) 720
BisI GCNGC 3 cut(s) 383, 648, 660
BlsI GCNGC 3 cut(s) 384, 649, 661
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 1 cut(s) 767
Bme18I GGWCC 2 cut(s) 496, 598
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 496, 598
BmrFI CCNGG 1 cut(s) 767
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 1 cut(s) 36
BmuI ACTGGG 1 cut(s) 553
BpmI CTGGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 767
Bsa29I ATCGAT 1 cut(s) 573
BsaAI YACGTR 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 433
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 269, 307, 439, 472
Bse1I ACTGG 3 cut(s) 499, 548, 799
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 2 cut(s) 364, 678
BseLI CCNNNNNNNGG 5 cut(s) 265, 269, 307, 439, 472
BseMII CTCAG 3 cut(s) 368, 386, 465
BseNI ACTGG 3 cut(s) 499, 548, 799
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 3 cut(s) 394, 634, 646
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 111
Bsh1285I CGRYCG 2 cut(s) 160, 283
BshFI GGCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 573
BsiEI CGRYCG 2 cut(s) 160, 283
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 2 cut(s) 176, 528
BslI CCNNNNNNNGG 5 cut(s) 265, 269, 307, 439, 472
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 176, 528
BsnI GGCC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 83, 355
Bsp143I GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
BspACI CCGC 1 cut(s) 111
BspANI GGCC 1 cut(s) 254
BspCNI CTCAG 3 cut(s) 369, 387, 464
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 1 cut(s) 111
BspPI GGATC 3 cut(s) 245, 533, 578
BsrI ACTGG 3 cut(s) 499, 548, 799
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
BssT1I CCWWGG 1 cut(s) 433
Bst4CI ACNGT 3 cut(s) 281, 341, 589
BstBAI YACGTR 1 cut(s) 97
BstDEI CTNAG 4 cut(s) 377, 395, 451, 611
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 364, 678
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 4 cut(s) 12, 111, 401, 837
BstKTI GATC 8 cut(s) 69, 160, 240, 528, 573, 577, 723, 760
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
BstMCI CGRYCG 2 cut(s) 160, 283
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstSCI CCNGG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 355
BstSNI TACGTA 1 cut(s) 97
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 394, 634, 646
BstX2I RGATCY 1 cut(s) 720
BstXI CCANNNNNNTGG 1 cut(s) 806
BstYI RGATCY 1 cut(s) 720
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 1 cut(s) 254
BsuTUI ATCGAT 1 cut(s) 573
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 2 cut(s) 364, 678
BtsIMutI CAGTG 1 cut(s) 541
CaiI CAGNNNCTG 1 cut(s) 376
CfoI GCGC 4 cut(s) 12, 111, 401, 837
Cfr13I GGNCC 2 cut(s) 496, 598
ClaI ATCGAT 1 cut(s) 573
CseI GACGC 1 cut(s) 190
Csp6I GTAC 4 cut(s) 56, 222, 333, 367
CviAII CATG 2 cut(s) 329, 781
CviJI RGCY 7 cut(s) 81, 134, 254, 385, 607, 659, 851
CviKI_1 RGCY 7 cut(s) 81, 134, 254, 385, 607, 659, 851
CviQI GTAC 4 cut(s) 56, 222, 333, 367
DdeI CTNAG 4 cut(s) 377, 395, 451, 611
DpnI GATC 8 cut(s) 68, 159, 239, 527, 572, 576, 722, 759
DpnII GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
Eco105I TACGTA 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 433
Eco147I AGGCCT 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 2 cut(s) 496, 598
Eco57I CTGAAG 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 2 cut(s) 332, 784
FaqI GGGAC 2 cut(s) 176, 528
FatI CATG 2 cut(s) 328, 780
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 3 cut(s) 383, 648, 660
FokI GGATG 2 cut(s) 371, 665
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 383, 648, 660
FspBI CTAG 1 cut(s) 665
FspI TGCGCA 1 cut(s) 836
GlaI GCGC 4 cut(s) 11, 110, 400, 836
GluI GCNGC 3 cut(s) 383, 648, 660
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 1 cut(s) 254
HapII CCGG 1 cut(s) 767
HgaI GACGC 1 cut(s) 190
HhaI GCGC 4 cut(s) 12, 111, 401, 837
Hin1II CATG 2 cut(s) 332, 784
Hin6I GCGC 4 cut(s) 10, 109, 399, 835
HinP1I GCGC 4 cut(s) 10, 109, 399, 835
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 2 cut(s) 103, 810
HpaII CCGG 1 cut(s) 767
HphI GGTGA 3 cut(s) 353, 449, 677
Hpy166II GTNNAC 2 cut(s) 598, 712
Hpy188I TCNGA 5 cut(s) 242, 276, 396, 454, 491
Hpy188III TCNNGA 2 cut(s) 161, 308
Hpy8I GTNNAC 2 cut(s) 598, 712
HpyAV CCTTC 8 cut(s) 263, 265, 412, 542, 558, 611, 619, 692
HpyCH4III ACNGT 3 cut(s) 281, 341, 589
HpyCH4IV ACGT 3 cut(s) 63, 96, 165
HpyCH4V TGCA 1 cut(s) 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpyF3I CTNAG 4 cut(s) 377, 395, 451, 611
HpySE526I ACGT 3 cut(s) 63, 96, 165
Hsp92II CATG 2 cut(s) 332, 784
HspAI GCGC 4 cut(s) 10, 109, 399, 835
Ksp22I TGATCA 1 cut(s) 757
Kzo9I GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
LpnPI CCDG 8 cut(s) 293, 377, 383, 474, 512, 529, 780, 812
Lsp1109I GCAGC 3 cut(s) 394, 634, 646
LweI GCATC 1 cut(s) 36
MaeI CTAG 1 cut(s) 665
MaeII ACGT 3 cut(s) 63, 96, 165
MaeIII GTNAC 1 cut(s) 632
MalI GATC 8 cut(s) 68, 159, 239, 527, 572, 576, 722, 759
MboI GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
MboII GAAGA 1 cut(s) 455
MflI RGATCY 1 cut(s) 720
MhlI GDGCHC 2 cut(s) 83, 355
MluCI AATT 5 cut(s) 114, 344, 478, 791, 839
MlyI GAGTC 1 cut(s) 819
MmeI TCCRAC 2 cut(s) 320, 723
MnlI CCTC 8 cut(s) 70, 269, 372, 433, 546, 561, 649, 698
MseI TTAA 2 cut(s) 170, 705
MslI CAYNNNNRTG 2 cut(s) 33, 435
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 1 cut(s) 767
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 656
NciI CCSGG 1 cut(s) 767
NdeII GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
NlaIII CATG 2 cut(s) 332, 784
NsbI TGCGCA 1 cut(s) 836
OliI CACNNNNGTG 1 cut(s) 435
PceI AGGCCT 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PfeI GAWTC 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 765
PkrI GCNGC 3 cut(s) 384, 649, 661
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 1 cut(s) 818
PpsI GAGTC 1 cut(s) 818
Ppu21I YACGTR 1 cut(s) 97
PsiI TTATAA 1 cut(s) 846
PspPI GGNCC 2 cut(s) 496, 598
PstNI CAGNNNCTG 1 cut(s) 376
PsuI RGATCY 1 cut(s) 720
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 4 cut(s) 57, 223, 334, 368
RsaNI GTAC 4 cut(s) 56, 222, 333, 367
RseI CAYNNNNRTG 2 cut(s) 33, 435
SaqAI TTAA 2 cut(s) 170, 705
SatI GCNGC 3 cut(s) 383, 648, 660
Sau3AI GATC 8 cut(s) 66, 157, 237, 525, 570, 574, 720, 757
Sau96I GGNCC 2 cut(s) 496, 598
ScaI AGTACT 1 cut(s) 223
SchI GAGTC 1 cut(s) 819
ScrFI CCNGG 1 cut(s) 767
SduI GDGCHC 2 cut(s) 83, 355
SfaNI GCATC 1 cut(s) 36
SinI GGWCC 2 cut(s) 496, 598
SmiMI CAYNNNNRTG 2 cut(s) 33, 435
SnaBI TACGTA 1 cut(s) 97
SpeI ACTAGT 1 cut(s) 664
Sse9I AATT 5 cut(s) 114, 344, 478, 791, 839
SseBI AGGCCT 1 cut(s) 254
SsiI CCGC 1 cut(s) 111
SspMI CTAG 1 cut(s) 665
StuI AGGCCT 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 765
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 3 cut(s) 281, 341, 589
TaiI ACGT 3 cut(s) 66, 99, 168
TaqI TCGA 3 cut(s) 69, 573, 813
TasI AATT 5 cut(s) 114, 344, 478, 791, 839
TatI WGTACW 1 cut(s) 221
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 170, 705
Tru9I TTAA 2 cut(s) 170, 705
TscAI CASTG 1 cut(s) 548
TseI GCWGC 3 cut(s) 382, 647, 659
TspDTI ATGAA 3 cut(s) 216, 317, 769
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 496, 598
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 4 cut(s) 344, 478, 791, 839
XspI CTAG 1 cut(s) 665
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.