Rroxscaffold_1G00058560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000544
Physical Location & Seq
Forward (+)
80475226 .. 80476427
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058560.1

Sequence Viewer

Length: 891 bp
ATGGTGAAGCGGAAGCGTCACACCGGCGGCAAGCCGGTTAGATTTTTCAAGGATGTGCTGGGAAGGAAATCTTATTATAGATTGTACAATTCGAATGGTGATTTCCTATGCTATGTAGCAAAGCCTTTAGACGTGCTGAATGCCCTCGTATATAGGTGGAAGCATAACCTTGGATGTAAGAAGTTGACCAGGTATAATGTTAGGGCGCGTGACAATCAAGCTTACCTACCTCAGGAAGGCTGTTCCAGTGACAGGGAGGATTATCCTAAGATATATCTGGAGTTTCTCCAAGATATATTTGACCCTGTTGCGGATGTCACAAGGGCCTGGGAAGATTTGTACAACAAGCTCTCTGACCCCGAGCTATCTCTGGAGAAATTTCGCAACCTTTTTTTCCACCTGTTTTTCCAGACGGAGGAGGAACTATGTGATTTCCCTGCCGTGGCAGTGTGGAATCTGCGGAACATAAAATTCGACCATGAAGGCTTGAAAGAAAACTGGACTGATGCTTATACCCGATGGGATGCTTTGCATCCCAGGGAGGGTGCAATAAACTTGATTCGGACCTGCAGGCAGAATGATTACTTCATGAGAGCTTACGAGTTCAAGAAATATGATAATGATGCTGTCAAGGAAGCAGAGCTGGTTAGAAATGGCATTACGCACTTTAACGAGAATGTAATGAAAGACGTGCCCCCTCCTGATGATTTCTTCAACAAACGCCAAATAGGAGAGGTCGTTCTTGATCTCTTCCCAGATGTCTCCTTCAGACTGTTTGATTTTATGTGGGACAATGATGTGAATATGGACTTCACTATGTCCCGCACATGTTTCAGGTACCACTTTGGGTTATTCATTAATTCGTTGTTTCTCTTATTGATACCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

35.43

Weight (kDa)

6.4

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 575
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 1 cut(s) 837
AccII CGCG 1 cut(s) 208
AciI CCGC 5 cut(s) 10, 27, 311, 460, 823
AcsI RAATTY 2 cut(s) 377, 470
AcuI CTGAAG 1 cut(s) 751
AfaI GTAC 3 cut(s) 86, 341, 839
AfiI CCNNNNNNNGG 7 cut(s) 232, 236, 252, 310, 415, 442, 542
AflIII ACRYGT 1 cut(s) 827
AgsI TTSAA 4 cut(s) 49, 490, 607, 715
AjiI CACGTC 2 cut(s) 133, 691
AjnI CCWGG 3 cut(s) 188, 326, 536
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
AluBI AGCT 5 cut(s) 221, 349, 364, 596, 643
AluI AGCT 5 cut(s) 221, 349, 364, 596, 643
Alw26I GTCTC 1 cut(s) 766
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 1 cut(s) 324
ApoI RAATTY 2 cut(s) 377, 470
AseI ATTAAT 1 cut(s) 858
Asp718I GGTACC 1 cut(s) 837
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 324, 564
AsuHPI GGTGA 2 cut(s) 16, 110
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 564
AxyI CCTNAGG 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 696
BanI GGYRCC 1 cut(s) 837
BccI CCATC 1 cut(s) 513
BceAI ACGGC 1 cut(s) 425
BciT130I CCWGG 3 cut(s) 190, 328, 538
BcoDI GTCTC 1 cut(s) 766
BfmI CTRYAG 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 1 cut(s) 28
BlsI GCNGC 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 190, 328, 538
Bme18I GGWCC 1 cut(s) 564
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 2 cut(s) 133, 691
BmgT120I GGNCC 2 cut(s) 324, 564
BmiI GGNNCC 1 cut(s) 839
BmrFI CCNGG 3 cut(s) 190, 328, 538
BmsI GCATC 4 cut(s) 496, 514, 541, 613
BpmI CTGGAG 2 cut(s) 299, 392
Bpu14I TTCGAA 1 cut(s) 92
BsaJI CCNNGG 5 cut(s) 169, 327, 441, 536, 537
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 7 cut(s) 232, 236, 252, 310, 415, 442, 542
Bse118I RCCGGY 2 cut(s) 23, 34
Bse1I ACTGG 2 cut(s) 246, 503
Bse21I CCTNAGG 1 cut(s) 231
BseBI CCWGG 3 cut(s) 190, 328, 538
BseDI CCNNGG 5 cut(s) 169, 327, 441, 536, 537
BseGI GGATG 5 cut(s) 58, 179, 319, 529, 532
BseLI CCNNNNNNNGG 7 cut(s) 232, 236, 252, 310, 415, 442, 542
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 2 cut(s) 246, 503
BseRI GAGGAG 1 cut(s) 431
BseSI GKGCMC 1 cut(s) 696
BseYI CCCAGC 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 326
BshNI GGYRCC 1 cut(s) 837
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 2 cut(s) 24, 35
BslFI GGGAC 2 cut(s) 803, 805
BslI CCNNNNNNNGG 7 cut(s) 232, 236, 252, 310, 415, 442, 542
BsmAI GTCTC 1 cut(s) 766
BsmFI GGGAC 2 cut(s) 803, 805
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 696
Bsp1407I TGTACA 2 cut(s) 84, 339
Bsp143I GATC 1 cut(s) 745
BspACI CCGC 5 cut(s) 10, 27, 311, 460, 823
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 244
BspFNI CGCG 1 cut(s) 208
BspHI TCATGA 1 cut(s) 588
BspLI GGNNCC 1 cut(s) 839
BspMAI CTGCAG 1 cut(s) 572
BspMI ACCTGC 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 837
BsrFI RCCGGY 2 cut(s) 23, 34
BsrGI TGTACA 2 cut(s) 84, 339
BsrI ACTGG 2 cut(s) 246, 503
BssAI RCCGGY 2 cut(s) 23, 34
BssECI CCNNGG 5 cut(s) 169, 327, 441, 536, 537
BssMI GATC 1 cut(s) 745
BssT1I CCWWGG 1 cut(s) 169
Bst2UI CCWGG 3 cut(s) 190, 328, 538
Bst4CI ACNGT 1 cut(s) 774
Bst6I CTCTTC 1 cut(s) 755
BstAUI TGTACA 2 cut(s) 84, 339
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 32, 572
BstDEI CTNAG 2 cut(s) 231, 267
BstDSI CCRYGG 1 cut(s) 441
BstENI CCTNNNNNAGG 2 cut(s) 230, 234
BstF5I GGATG 5 cut(s) 58, 179, 319, 529, 532
BstFNI CGCG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 1 cut(s) 748
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 1 cut(s) 745
BstNI CCWGG 3 cut(s) 190, 328, 538
BstNSI RCATGY 1 cut(s) 831
BstSCI CCNGG 3 cut(s) 188, 326, 536
BstSFI CTRYAG 1 cut(s) 568
BstSLI GKGCMC 1 cut(s) 696
BstUI CGCG 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 231
BsuRI GGCC 1 cut(s) 326
BtgI CCRYGG 1 cut(s) 441
BtrI CACGTC 2 cut(s) 133, 691
BtsCI GGATG 5 cut(s) 58, 179, 319, 529, 532
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 253, 453
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 32, 572
CciI TCATGA 1 cut(s) 588
CfoI GCGC 1 cut(s) 208
Cfr10I RCCGGY 2 cut(s) 23, 34
Cfr13I GGNCC 2 cut(s) 324, 564
CseI GACGC 1 cut(s) 5
CsiI ACCWGGT 1 cut(s) 188
Csp6I GTAC 3 cut(s) 85, 340, 838
CviAII CATG 3 cut(s) 479, 589, 828
CviQI GTAC 3 cut(s) 85, 340, 838
DdeI CTNAG 2 cut(s) 231, 267
DpnI GATC 1 cut(s) 747
DpnII GATC 1 cut(s) 745
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 169
Eco47I GGWCC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 751
Eco81I CCTNAGG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 359
EcoNI CCTNNNNNAGG 2 cut(s) 230, 234
EcoO109I RGGNCCY 1 cut(s) 324
EcoRII CCWGG 3 cut(s) 188, 326, 536
EcoT14I CCWWGG 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 3 cut(s) 482, 592, 831
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 2 cut(s) 803, 805
FatI CATG 3 cut(s) 478, 588, 827
FauI CCCGC 1 cut(s) 830
Fnu4HI GCNGC 1 cut(s) 28
FokI GGATG 5 cut(s) 65, 186, 326, 519, 536
Fsp4HI GCNGC 1 cut(s) 28
GlaI GCGC 1 cut(s) 207
GluI GCNGC 1 cut(s) 28
GsaI CCCAGC 1 cut(s) 62
GsuI CTGGAG 2 cut(s) 299, 392
HaeIII GGCC 1 cut(s) 326
HapII CCGG 2 cut(s) 24, 35
HgaI GACGC 1 cut(s) 5
HhaI GCGC 1 cut(s) 208
Hin1II CATG 3 cut(s) 482, 592, 831
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HincII GTYRAC 1 cut(s) 186
HindII GTYRAC 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 2 cut(s) 454, 559
HpaII CCGG 2 cut(s) 24, 35
HphI GGTGA 2 cut(s) 16, 110
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 3 cut(s) 355, 564, 770
Hpy188III TCNNGA 8 cut(s) 233, 278, 371, 409, 589, 607, 701, 743
Hpy8I GTNNAC 1 cut(s) 186
HpyAV CCTTC 4 cut(s) 57, 230, 476, 775
HpyCH4III ACNGT 1 cut(s) 774
HpyCH4IV ACGT 2 cut(s) 132, 690
HpyCH4V TGCA 3 cut(s) 532, 548, 570
HpyF3I CTNAG 2 cut(s) 231, 267
HpySE526I ACGT 2 cut(s) 132, 690
Hsp92II CATG 3 cut(s) 482, 592, 831
HspAI GCGC 1 cut(s) 206
KpnI GGTACC 1 cut(s) 841
Kzo9I GATC 1 cut(s) 745
LweI GCATC 4 cut(s) 496, 514, 541, 613
MabI ACCWGGT 1 cut(s) 188
MaeII ACGT 2 cut(s) 132, 690
MaeIII GTNAC 4 cut(s) 17, 209, 248, 316
MalI GATC 1 cut(s) 747
MboI GATC 1 cut(s) 745
MboII GAAGA 3 cut(s) 344, 703, 742
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 4 cut(s) 88, 377, 470, 859
MnlI CCTC 8 cut(s) 155, 240, 250, 409, 412, 535, 708, 727
MseI TTAA 2 cut(s) 669, 858
MspI CCGG 2 cut(s) 24, 35
MspR9I CCNGG 3 cut(s) 190, 328, 538
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 3 cut(s) 190, 328, 538
MvnI CGCG 1 cut(s) 208
NdeII GATC 1 cut(s) 745
NlaIII CATG 3 cut(s) 482, 592, 831
NlaIV GGNNCC 1 cut(s) 839
NmuCI GTSAC 4 cut(s) 17, 209, 248, 316
NspI RCATGY 1 cut(s) 831
NspV TTCGAA 1 cut(s) 92
PagI TCATGA 1 cut(s) 588
PasI CCCWGGG 1 cut(s) 537
PciI ACATGT 1 cut(s) 827
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 2 cut(s) 454, 559
PkrI GCNGC 1 cut(s) 29
PscI ACATGT 1 cut(s) 827
PshBI ATTAAT 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 188, 326, 536
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 3 cut(s) 188, 326, 536
PspN4I GGNNCC 1 cut(s) 839
PspPI GGNCC 2 cut(s) 324, 564
PstI CTGCAG 1 cut(s) 572
RsaI GTAC 3 cut(s) 86, 341, 839
RsaNI GTAC 3 cut(s) 85, 340, 838
SaqAI TTAA 2 cut(s) 669, 858
SatI GCNGC 1 cut(s) 28
Sau3AI GATC 1 cut(s) 745
Sau96I GGNCC 2 cut(s) 324, 564
SbfI CCTGCAGG 1 cut(s) 572
ScrFI CCNGG 3 cut(s) 190, 328, 538
SdaI CCTGCAGG 1 cut(s) 572
SduI GDGCHC 1 cut(s) 696
SexAI ACCWGGT 1 cut(s) 188
SfaNI GCATC 4 cut(s) 496, 514, 541, 613
SfcI CTRYAG 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 92
SgrAI CRCCGGYG 1 cut(s) 23
SinI GGWCC 1 cut(s) 564
Sse8387I CCTGCAGG 1 cut(s) 572
Sse9I AATT 4 cut(s) 88, 377, 470, 859
SsiI CCGC 5 cut(s) 10, 27, 311, 460, 823
StyD4I CCNGG 3 cut(s) 188, 326, 536
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 774
TaiI ACGT 2 cut(s) 135, 693
TaqI TCGA 2 cut(s) 92, 474
TasI AATT 4 cut(s) 88, 377, 470, 859
TatI WGTACW 2 cut(s) 84, 339
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 2 cut(s) 454, 559
Tru1I TTAA 2 cut(s) 669, 858
Tru9I TTAA 2 cut(s) 669, 858
TscAI CASTG 2 cut(s) 253, 453
TseFI GTSAC 4 cut(s) 17, 209, 248, 316
Tsp45I GTSAC 4 cut(s) 17, 209, 248, 316
TspDTI ATGAA 4 cut(s) 495, 577, 698, 844
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 2 cut(s) 253, 453
VpaK11BI GGWCC 1 cut(s) 564
VspI ATTAAT 1 cut(s) 858
XagI CCTNNNNNAGG 2 cut(s) 230, 234
XapI RAATTY 2 cut(s) 377, 470
XceI RCATGY 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.