Rorug07G0229300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000544
Physical Location & Seq
Forward (+)
20999734 .. 21001564
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0229300.1

Sequence Viewer

Length: 1626 bp
ATGGACAACAAAGATGATGATCACCCTGCAAGACAATATGAGGATTATGAGCAATCTTTCTATACTCCAAGTATTCCACAGGTTACTCCTTCTCCAATCTACATTCCACAACCATATCGCCCTGCACAACAATATGAACATCCTCGATATTCTCCTTGCTCTCCGTCTTACATTCCACAACCATGTCACCCAACACAACAATATGAACATCCTCGATATTCTCCTAGCTCTCCATCCAACATTCCACAACCATATCACCCTACACGACAATATGAACACCCTCGGTATTCTCCTTTCCCTCCATCATACTCTCCATGTTCTCCTTCCTCTCCACCATACTTTTCACAGCGATATCACCCTACAATACAATTTGAACTCCCTAGGTATTCTCCTGGTATAGATCCTTCCCCTGGCGGGGGTCATGTGACCAGTAGTTATCCACTGCCTTCCTTCAAACCATCTAGGGAACTCCGAGAATCCATTAAAAACCAAACCGATGTTGCACTGGAAATCACAAAGAAACTGCTTCTAACTTTAGGCAAGGCCAAGAACATGGTGTACTCCCCATTGTCCATCCACATTGGTCTTGGCATGATATTGACTGGGACAAAGGGTCATATCCAGGACCGGTTTCTCTCTTTCCTCAAGTCCAAGTCCATCAATGAGCTCAATGATCTCGCCTCCAATGTCTATCCACTGGTCTTTGCCGACGGATACTCAAAGGGCGGGCCTCGCTTTTCAGTCGCCAATGGTGTTTGGGTTGAAAAGTCTCTCCATGTCAAGCCTTGTTTCAAAGAGGTACTGGACACTGCTTACAAGGCAGCAATGAATCAAGTCGATTTTCGAAGAAGGGCAGACGAAGTGCGATGTGAAGTGAATTCATGGGTAGACAAGGAGACCAATGGACTTATCAAAGAGATTCTAGCCGCAGGGTCTGTTAGCAGCGAAACAAAGCTCATCCTTGCGAATGCCTTATACTTCAAAGGAGCGTGGAAGGAGAAGTTCTATGAATCAATGACAAACGAGTTTGATTTCCATCTACAGAGTGGGAGCTCAGTTAAGGCACCCTTCATGACCAGTTCGAAGTACCAGTTTGTAAGTGTCTTTGACAGCTTCAAAGTCTTAAAGCTTCCCTACGAGCAAGGTAAAGATTATGGTCGTCGTTTCTCCATGTGCTTGTTTCTTCCAAATGCAACTGATGGACTACAAGCTTTGGTTGAGAGGGTTTGTTCCGAGCCTATAGATCGATATATTCCCCACAAAAATGTTCCACTTCGTAGATTTTTAATCCCAAAGTTTAAGATATCTGTTGGGTTTGATCCTATGGATGTTCTGAAACCGTTAGGATTCTCTCTTGAAGAGGGAGATTTGACAGAGATGGTGGAGGGTGCGATTTCTCTCTCCATGTTCCAGAAATCTTTCATTGAAGTTAATGAAGAAGGCACCGAGGCTGCTGCTGTTTATATTGCCTGCGGTCCTGCTTATTCATCGGGTGAACCACCCAAACCTCCTCCGATAGATTTTGTGGCAGATCACCCATTCCTTTATCTGATTAGAGAAGAGGTGACTGGAACGGTCATGTTCATTGGGCACGTCCTAAACCCCATTGAAGAAAAATTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

541

Amino Acids

61.22

Weight (kDa)

6.45

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 163 - 535 8e-86 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1063, 1442
AccI GTMKAC 1 cut(s) 888
AciI CCGC 4 cut(s) 414, 726, 927, 1473
AclWI GGATC 2 cut(s) 395, 1313
AcsI RAATTY 2 cut(s) 877, 1616
AfaI GTAC 3 cut(s) 560, 801, 1088
AfiI CCNNNNNNNGG 4 cut(s) 410, 414, 415, 416
AgeI ACCGGT 1 cut(s) 627
AgsI TTSAA 9 cut(s) 374, 454, 764, 793, 982, 1117, 1358, 1427, 1610
AhdI GACNNNNNGTC 1 cut(s) 612
AjiI CACGTC 1 cut(s) 1594
AjnI CCWGG 3 cut(s) 391, 409, 621
AluBI AGCT 7 cut(s) 228, 667, 955, 1053, 1113, 1129, 1211
AluI AGCT 7 cut(s) 228, 667, 955, 1053, 1113, 1129, 1211
Alw21I GWGCWC 2 cut(s) 669, 1055
Alw26I GTCTC 2 cut(s) 774, 890
AlwI GGATC 2 cut(s) 395, 1313
AlwNI CAGNNNCTG 1 cut(s) 935
AoxI GGCC 2 cut(s) 543, 728
ApeKI GCWGC 4 cut(s) 821, 942, 1451, 1454
ApoI RAATTY 2 cut(s) 877, 1616
AsiGI ACCGGT 1 cut(s) 627
Asp700I GAANNNNTTC 1 cut(s) 1418
AspA2I CCTAGG 1 cut(s) 380
AspS9I GGNCC 3 cut(s) 625, 728, 1475
AsuHPI GGTGA 8 cut(s) 14, 179, 248, 347, 1505, 1526, 1576, 1612
AsuII TTCGAA 2 cut(s) 844, 1082
AvaII GGWCC 2 cut(s) 625, 1475
AvrII CCTAGG 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 1593
BanI GGYRCC 2 cut(s) 1063, 1442
BanII GRGCYC 2 cut(s) 669, 1055
BarI GAAGNNNNNNTAC 2 cut(s) 388, 420
Bbv12I GWGCWC 2 cut(s) 669, 1055
BbvI GCAGC 4 cut(s) 833, 954, 1438, 1441
BccI CCATC 8 cut(s) 241, 310, 466, 581, 665, 1044, 1193, 1372
BcgI CGANNNNNNTGC 2 cut(s) 1436, 1470
BciT130I CCWGG 3 cut(s) 393, 411, 623
BciVI GTATCC 1 cut(s) 707
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 2 cut(s) 774, 890
BfaI CTAG 4 cut(s) 225, 381, 462, 923
BfmI CTRYAG 2 cut(s) 1040, 1239
BfuI GTATCC 1 cut(s) 707
BisI GCNGC 5 cut(s) 822, 927, 943, 1452, 1455
BlnI CCTAGG 1 cut(s) 380
BlsI GCNGC 5 cut(s) 823, 928, 944, 1453, 1456
Bme1390I CCNGG 3 cut(s) 393, 411, 623
Bme18I GGWCC 2 cut(s) 625, 1475
BmeRI GACNNNNNGTC 1 cut(s) 612
BmgBI CACGTC 1 cut(s) 1594
BmgT120I GGNCC 3 cut(s) 625, 728, 1475
BmiI GGNNCC 2 cut(s) 1065, 1444
BmrFI CCNGG 3 cut(s) 393, 411, 623
BmrI ACTGGG 1 cut(s) 612
BmuI ACTGGG 1 cut(s) 612
Bpu14I TTCGAA 2 cut(s) 844, 1082
BpuEI CTTGAG 1 cut(s) 629
Bsa29I ATCGAT 1 cut(s) 1246
BsaBI GATNNNNATC 2 cut(s) 18, 1035
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 4 cut(s) 281, 380, 409, 1446
BsaWI WCCGGW 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 414, 415, 416
Bse118I RCCGGY 1 cut(s) 627
Bse1I ACTGG 8 cut(s) 429, 510, 607, 702, 807, 1077, 1090, 1573
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 2 cut(s) 18, 1035
BseBI CCWGG 3 cut(s) 393, 411, 623
BseCI ATCGAT 1 cut(s) 1246
BseDI CCNNGG 4 cut(s) 281, 380, 409, 1446
BseGI GGATG 6 cut(s) 139, 208, 233, 573, 957, 1333
BseJI GATNNNNATC 2 cut(s) 18, 1035
BseLI CCNNNNNNNGG 4 cut(s) 410, 414, 415, 416
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 1 cut(s) 1068
BseNI ACTGG 8 cut(s) 429, 510, 607, 702, 807, 1077, 1090, 1573
BseRI GAGGAG 1 cut(s) 1500
BseSI GKGCMC 1 cut(s) 1593
BseXI GCAGC 4 cut(s) 833, 954, 1438, 1441
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 2 cut(s) 545, 730
BshNI GGYRCC 2 cut(s) 1063, 1442
BshTI ACCGGT 1 cut(s) 627
BshVI ATCGAT 1 cut(s) 1246
BsiHKAI GWGCWC 2 cut(s) 669, 1055
BsiSI CCGG 1 cut(s) 628
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 4 cut(s) 410, 414, 415, 416
BsmAI GTCTC 2 cut(s) 774, 890
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 2 cut(s) 545, 730
Bso31I GGTCTC 1 cut(s) 890
Bsp119I TTCGAA 2 cut(s) 844, 1082
Bsp1286I GDGCHC 3 cut(s) 669, 1055, 1593
Bsp143I GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
BspACI CCGC 4 cut(s) 414, 726, 927, 1473
BspANI GGCC 2 cut(s) 545, 730
BspCNI CTCAG 1 cut(s) 1067
BspDI ATCGAT 1 cut(s) 1246
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 2 cut(s) 1065, 1444
BspPI GGATC 2 cut(s) 395, 1313
BspT104I TTCGAA 2 cut(s) 844, 1082
BspT107I GGYRCC 2 cut(s) 1063, 1442
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 1 cut(s) 627
BsrI ACTGG 8 cut(s) 429, 510, 607, 702, 807, 1077, 1090, 1573
BssAI RCCGGY 1 cut(s) 627
BssECI CCNNGG 4 cut(s) 281, 380, 409, 1446
BssMI GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 3 cut(s) 393, 411, 623
Bst4CI ACNGT 2 cut(s) 1341, 1576
Bst6I CTCTTC 2 cut(s) 1353, 1554
BstBI TTCGAA 2 cut(s) 844, 1082
BstC8I GCNNGC 2 cut(s) 728, 1471
BstDEI CTNAG 1 cut(s) 1054
BstF5I GGATG 6 cut(s) 139, 208, 233, 573, 957, 1333
BstKTI GATC 6 cut(s) 22, 403, 676, 1246, 1321, 1534
BstMAI GTCTC 2 cut(s) 774, 890
BstMBI GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
BstMWI GCNNNNNNNGC 2 cut(s) 732, 818
BstNI CCWGG 3 cut(s) 393, 411, 623
BstSCI CCNGG 3 cut(s) 391, 409, 621
BstSFI CTRYAG 2 cut(s) 1040, 1239
BstSLI GKGCMC 1 cut(s) 1593
BstV1I GCAGC 4 cut(s) 833, 954, 1438, 1441
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 1246
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 2 cut(s) 545, 730
BsuTUI ATCGAT 1 cut(s) 1246
BtgZI GCGATG 1 cut(s) 880
BtrI CACGTC 1 cut(s) 1594
BtsCI GGATG 6 cut(s) 139, 208, 233, 573, 957, 1333
BtsI GCAGTG 2 cut(s) 440, 807
BtsIMutI CAGTG 4 cut(s) 440, 503, 695, 807
Cac8I GCNNGC 2 cut(s) 728, 1471
CaiI CAGNNNCTG 1 cut(s) 935
CciI TCATGA 1 cut(s) 1071
Cfr10I RCCGGY 1 cut(s) 627
Cfr13I GGNCC 3 cut(s) 625, 728, 1475
ClaI ATCGAT 1 cut(s) 1246
Csp6I GTAC 3 cut(s) 559, 800, 1087
CspAI ACCGGT 1 cut(s) 627
CviQI GTAC 3 cut(s) 559, 800, 1087
DdeI CTNAG 1 cut(s) 1054
DpnI GATC 6 cut(s) 21, 402, 675, 1245, 1320, 1533
DpnII GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
DriI GACNNNNNGTC 1 cut(s) 612
Eam1104I CTCTTC 2 cut(s) 1353, 1554
Eam1105I GACNNNNNGTC 1 cut(s) 612
EarI CTCTTC 2 cut(s) 1353, 1554
Ecl136II GAGCTC 2 cut(s) 667, 1053
Eco130I CCWWGG 1 cut(s) 380
Eco24I GRGCYC 2 cut(s) 669, 1055
Eco31I GGTCTC 1 cut(s) 890
Eco32I GATATC 2 cut(s) 353, 1305
Eco47I GGWCC 2 cut(s) 625, 1475
Eco53kI GAGCTC 2 cut(s) 667, 1053
EcoICRI GAGCTC 2 cut(s) 667, 1053
EcoRI GAATTC 1 cut(s) 877
EcoRII CCWGG 3 cut(s) 391, 409, 621
EcoRV GATATC 2 cut(s) 353, 1305
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 2 cut(s) 669, 1055
ErhI CCWWGG 1 cut(s) 380
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 1 cut(s) 619
FauI CCCGC 2 cut(s) 407, 719
FbaI TGATCA 1 cut(s) 19
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 5 cut(s) 822, 927, 943, 1452, 1455
FokI GGATG 6 cut(s) 126, 195, 220, 560, 944, 1340
FriOI GRGCYC 2 cut(s) 669, 1055
Fsp4HI GCNGC 5 cut(s) 822, 927, 943, 1452, 1455
FspBI CTAG 4 cut(s) 225, 381, 462, 923
GluI GCNGC 5 cut(s) 822, 927, 943, 1452, 1455
HaeIII GGCC 2 cut(s) 545, 730
HapII CCGG 1 cut(s) 628
HindIII AAGCTT 2 cut(s) 1127, 1209
HinfI GANTC 5 cut(s) 476, 829, 919, 1010, 1347
HpaII CCGG 1 cut(s) 628
HphI GGTGA 8 cut(s) 14, 179, 248, 347, 1505, 1526, 1576, 1612
Hpy166II GTNNAC 3 cut(s) 559, 889, 1496
Hpy188I TCNGA 5 cut(s) 473, 1234, 1335, 1515, 1551
Hpy188III TCNNGA 3 cut(s) 1072, 1355, 1411
Hpy8I GTNNAC 3 cut(s) 559, 889, 1496
Hpy99I CGWCG 2 cut(s) 713, 1164
HpyAV CCTTC 9 cut(s) 99, 333, 414, 456, 460, 843, 988, 1078, 1433
HpyCH4III ACNGT 2 cut(s) 1341, 1576
HpyCH4IV ACGT 1 cut(s) 1593
HpyCH4V TGCA 4 cut(s) 29, 125, 503, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 732, 818
HpyF3I CTNAG 1 cut(s) 1054
HpySE526I ACGT 1 cut(s) 1593
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
LmnI GCTCC 2 cut(s) 986, 1050
Lsp1109I GCAGC 4 cut(s) 833, 954, 1438, 1441
MaeI CTAG 4 cut(s) 225, 381, 462, 923
MaeII ACGT 1 cut(s) 1593
MaeIII GTNAC 4 cut(s) 82, 185, 424, 1564
MalI GATC 6 cut(s) 21, 402, 675, 1245, 1320, 1533
MboI GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
MboII GAAGA 6 cut(s) 858, 1175, 1370, 1448, 1571, 1622
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 3 cut(s) 669, 1055, 1593
MluCI AATT 3 cut(s) 368, 877, 1616
MmeI TCCRAC 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 1418
MseI TTAA 6 cut(s) 483, 1059, 1124, 1286, 1299, 1431
MslI CAYNNNNRTG 2 cut(s) 181, 1263
MspI CCGG 1 cut(s) 628
MspR9I CCNGG 3 cut(s) 393, 411, 623
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 3 cut(s) 393, 411, 623
MwoI GCNNNNNNNGC 2 cut(s) 732, 818
NdeII GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
NlaIV GGNNCC 2 cut(s) 1065, 1444
NmuCI GTSAC 3 cut(s) 185, 424, 1564
NspV TTCGAA 2 cut(s) 844, 1082
PagI TCATGA 1 cut(s) 1071
PctI GAATGC 1 cut(s) 973
PdmI GAANNNNTTC 1 cut(s) 1418
PfeI GAWTC 5 cut(s) 476, 829, 919, 1010, 1347
PfoI TCCNGGA 1 cut(s) 621
PinAI ACCGGT 1 cut(s) 627
PkrI GCNGC 5 cut(s) 823, 928, 944, 1453, 1456
Psp124BI GAGCTC 2 cut(s) 669, 1055
Psp6I CCWGG 3 cut(s) 391, 409, 621
PspGI CCWGG 3 cut(s) 391, 409, 621
PspN4I GGNNCC 2 cut(s) 1065, 1444
PspPI GGNCC 3 cut(s) 625, 728, 1475
PsrI GAACNNNNNNTAC 2 cut(s) 542, 574
PstNI CAGNNNCTG 1 cut(s) 935
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 3 cut(s) 560, 801, 1088
RsaNI GTAC 3 cut(s) 559, 800, 1087
RseI CAYNNNNRTG 2 cut(s) 181, 1263
SacI GAGCTC 2 cut(s) 669, 1055
SaqAI TTAA 6 cut(s) 483, 1059, 1124, 1286, 1299, 1431
SatI GCNGC 5 cut(s) 822, 927, 943, 1452, 1455
Sau3AI GATC 6 cut(s) 19, 400, 673, 1243, 1318, 1531
Sau96I GGNCC 3 cut(s) 625, 728, 1475
ScrFI CCNGG 3 cut(s) 393, 411, 623
SduI GDGCHC 3 cut(s) 669, 1055, 1593
SfcI CTRYAG 2 cut(s) 1040, 1239
SfuI TTCGAA 2 cut(s) 844, 1082
SinI GGWCC 2 cut(s) 625, 1475
SmiMI CAYNNNNRTG 2 cut(s) 181, 1263
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 3 cut(s) 368, 877, 1616
SsiI CCGC 4 cut(s) 414, 726, 927, 1473
SspMI CTAG 4 cut(s) 225, 381, 462, 923
SstI GAGCTC 2 cut(s) 669, 1055
StyD4I CCNGG 3 cut(s) 391, 409, 621
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 2 cut(s) 1341, 1576
TaiI ACGT 1 cut(s) 1596
TaqI TCGA 6 cut(s) 145, 214, 837, 844, 1082, 1246
TasI AATT 3 cut(s) 368, 877, 1616
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 929
TfiI GAWTC 5 cut(s) 476, 829, 919, 1010, 1347
Tru1I TTAA 6 cut(s) 483, 1059, 1124, 1286, 1299, 1431
Tru9I TTAA 6 cut(s) 483, 1059, 1124, 1286, 1299, 1431
TscAI CASTG 4 cut(s) 447, 510, 702, 814
TseFI GTSAC 3 cut(s) 185, 424, 1564
TseI GCWGC 4 cut(s) 821, 942, 1451, 1454
Tsp45I GTSAC 3 cut(s) 185, 424, 1564
TspGWI ACGGA 2 cut(s) 153, 726
TspRI CASTG 4 cut(s) 447, 510, 702, 814
VpaK11BI GGWCC 2 cut(s) 625, 1475
XapI RAATTY 2 cut(s) 877, 1616
XcmI CCANNNNNNNNNTGG 2 cut(s) 584, 1043
XmaJI CCTAGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 1418
XspI CTAG 4 cut(s) 225, 381, 462, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.