The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 270 |
| AclWI | GGATC | 1 cut(s) | 79 |
| AcoI | YGGCCR | 1 cut(s) | 111 |
| AluBI | AGCT | 1 cut(s) | 301 |
| AluI | AGCT | 1 cut(s) | 301 |
| Alw21I | GWGCWC | 1 cut(s) | 303 |
| Alw26I | GTCTC | 1 cut(s) | 244 |
| AlwI | GGATC | 1 cut(s) | 79 |
| AoxI | GGCC | 2 cut(s) | 27, 111 |
| AspS9I | GGNCC | 1 cut(s) | 27 |
| BanII | GRGCYC | 1 cut(s) | 303 |
| Bbv12I | GWGCWC | 1 cut(s) | 303 |
| BccI | CCATC | 1 cut(s) | 86 |
| BceAI | ACGGC | 1 cut(s) | 98 |
| BcoDI | GTCTC | 1 cut(s) | 244 |
| BfaI | CTAG | 1 cut(s) | 278 |
| BmgT120I | GGNCC | 1 cut(s) | 27 |
| BmrI | ACTGGG | 1 cut(s) | 34 |
| BmsI | GCATC | 2 cut(s) | 161, 180 |
| BmuI | ACTGGG | 1 cut(s) | 34 |
| BpuEI | CTTGAG | 1 cut(s) | 132 |
| BsaBI | GATNNNNATC | 1 cut(s) | 141 |
| Bse1I | ACTGG | 3 cut(s) | 29, 178, 337 |
| Bse8I | GATNNNNATC | 1 cut(s) | 141 |
| BseGI | GGATG | 2 cut(s) | 238, 295 |
| BseJI | GATNNNNATC | 1 cut(s) | 141 |
| BseNI | ACTGG | 3 cut(s) | 29, 178, 337 |
| BshFI | GGCC | 2 cut(s) | 29, 113 |
| BsiHKAI | GWGCWC | 1 cut(s) | 303 |
| BsmAI | GTCTC | 1 cut(s) | 244 |
| BsnI | GGCC | 2 cut(s) | 29, 113 |
| Bsp1286I | GDGCHC | 1 cut(s) | 303 |
| Bsp143I | GATC | 2 cut(s) | 71, 136 |
| BspANI | GGCC | 2 cut(s) | 29, 113 |
| BspPI | GGATC | 1 cut(s) | 79 |
| BsrI | ACTGG | 3 cut(s) | 29, 178, 337 |
| BssMI | GATC | 2 cut(s) | 71, 136 |
| Bst4CI | ACNGT | 5 cut(s) | 52, 108, 134, 257, 353 |
| Bst6I | CTCTTC | 1 cut(s) | 247 |
| BstF5I | GGATG | 2 cut(s) | 238, 295 |
| BstKTI | GATC | 2 cut(s) | 74, 139 |
| BstMAI | GTCTC | 1 cut(s) | 244 |
| BstMBI | GATC | 2 cut(s) | 71, 136 |
| BsuRI | GGCC | 2 cut(s) | 29, 113 |
| BtsCI | GGATG | 2 cut(s) | 238, 295 |
| BtsIMutI | CAGTG | 1 cut(s) | 262 |
| Cfr13I | GGNCC | 1 cut(s) | 27 |
| CviJI | RGCY | 3 cut(s) | 29, 113, 301 |
| CviKI_1 | RGCY | 3 cut(s) | 29, 113, 301 |
| DpnI | GATC | 2 cut(s) | 73, 138 |
| DpnII | GATC | 2 cut(s) | 71, 136 |
| EaeI | YGGCCR | 1 cut(s) | 111 |
| Eam1104I | CTCTTC | 1 cut(s) | 247 |
| EarI | CTCTTC | 1 cut(s) | 247 |
| Ecl136II | GAGCTC | 1 cut(s) | 301 |
| Eco24I | GRGCYC | 1 cut(s) | 303 |
| Eco53kI | GAGCTC | 1 cut(s) | 301 |
| EcoICRI | GAGCTC | 1 cut(s) | 301 |
| EcoT38I | GRGCYC | 1 cut(s) | 303 |
| FaiI | YATR | 3 cut(s) | 36, 158, 340 |
| FblI | GTMKAC | 1 cut(s) | 270 |
| FokI | GGATG | 2 cut(s) | 245, 302 |
| FriOI | GRGCYC | 1 cut(s) | 303 |
| FspBI | CTAG | 1 cut(s) | 278 |
| HaeIII | GGCC | 2 cut(s) | 29, 113 |
| HincII | GTYRAC | 1 cut(s) | 271 |
| HindII | GTYRAC | 1 cut(s) | 271 |
| HinfI | GANTC | 1 cut(s) | 167 |
| Hpy166II | GTNNAC | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 1 cut(s) | 271 |
| Hpy99I | CGWCG | 1 cut(s) | 275 |
| HpyAV | CCTTC | 2 cut(s) | 112, 255 |
| HpyCH4III | ACNGT | 5 cut(s) | 52, 108, 134, 257, 353 |
| Kzo9I | GATC | 2 cut(s) | 71, 136 |
| LmnI | GCTCC | 1 cut(s) | 306 |
| LpnPI | CCDG | 3 cut(s) | 10, 191, 318 |
| LweI | GCATC | 2 cut(s) | 161, 180 |
| MaeI | CTAG | 1 cut(s) | 278 |
| MalI | GATC | 2 cut(s) | 73, 138 |
| MboI | GATC | 2 cut(s) | 71, 136 |
| MboII | GAAGA | 1 cut(s) | 234 |
| MhlI | GDGCHC | 1 cut(s) | 303 |
| MluCI | AATT | 1 cut(s) | 210 |
| MnlI | CCTC | 3 cut(s) | 156, 222, 280 |
| MseI | TTAA | 2 cut(s) | 173, 225 |
| NdeII | GATC | 2 cut(s) | 71, 136 |
| PfeI | GAWTC | 1 cut(s) | 167 |
| Psp124BI | GAGCTC | 1 cut(s) | 303 |
| PspPI | GGNCC | 1 cut(s) | 27 |
| SacI | GAGCTC | 1 cut(s) | 303 |
| SalI | GTCGAC | 1 cut(s) | 269 |
| SaqAI | TTAA | 2 cut(s) | 173, 225 |
| Sau3AI | GATC | 2 cut(s) | 71, 136 |
| Sau96I | GGNCC | 1 cut(s) | 27 |
| SduI | GDGCHC | 1 cut(s) | 303 |
| SetI | ASST | 2 cut(s) | 283, 303 |
| SfaNI | GCATC | 2 cut(s) | 161, 180 |
| SgeI | CNNG | 8 cut(s) | 37, 43, 127, 161, 190, 290, 335, 345 |
| SmlI | CTYRAG | 1 cut(s) | 147 |
| SmoI | CTYRAG | 1 cut(s) | 147 |
| Sse9I | AATT | 1 cut(s) | 210 |
| SspMI | CTAG | 1 cut(s) | 278 |
| SstI | GAGCTC | 1 cut(s) | 303 |
| TaaI | ACNGT | 5 cut(s) | 52, 108, 134, 257, 353 |
| TaqI | TCGA | 1 cut(s) | 270 |
| TasI | AATT | 1 cut(s) | 210 |
| TfiI | GAWTC | 1 cut(s) | 167 |
| Tru1I | TTAA | 2 cut(s) | 173, 225 |
| Tru9I | TTAA | 2 cut(s) | 173, 225 |
| TscAI | CASTG | 1 cut(s) | 262 |
| TspDTI | ATGAA | 2 cut(s) | 54, 180 |
| TspRI | CASTG | 1 cut(s) | 262 |
| XmiI | GTMKAC | 1 cut(s) | 270 |
| XspI | CTAG | 1 cut(s) | 278 |