RLG00000032446

Haloacid dehalogenase-like hydrolase domain-containing protein At3g48420

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000544
Physical Location & Seq
Reverse (-)
15352686 .. 15354091
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032446

Sequence Viewer

Length: 531 bp
ATGAAGATATGGACAACCTTCCTTGAGCCAGTGCTTGGTTTCCCTCCTCGGCCTCAGGTTGCAGAGGATACTGAAGATGTTGTGAAGGCAAAAACTCATGCTGGCAAAAATGGTGCTCTGTTAATCAAACACTGTGGACTCCCAGTGGACCAACACACCAATGGCCATGGAATTCGCTTCCTGCTCAATTCTGAGGCCTCAGTCTTTTTCAACCATCTTTCACCATACCAACTTCACCTCTTTGCTTTACCCAAGAACCATCTCAACTTGCCCTCTCCATTTTCGCCAACTTTCCCAAAAAACTCATCCTTTCCTGGAAAATGTCGCCACTTTCATCGCTTCAATGCTTTCAGCTCACTTTCCGGCGCCAACTACCAGAACCCATCTGCGCAACTCGCCGTTCTTCTTGATGTTGACGGGGTTCTTATGGATGTATATCACTTGGGTAACCACCAGACATTCAATGTAGTTTTTAAAAAGCTTGGGCTTGACTGTGCAATTGGAGTGAACCTGTTTATACAGACCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.52

Weight (kDa)

8.83

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 365
AccB7I CCANNNNNTGG 1 cut(s) 35
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 366
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 3 cut(s) 211, 343, 463
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 2 cut(s) 354, 481
AluI AGCT 2 cut(s) 354, 481
Alw21I GWGCWC 1 cut(s) 118
AoxI GGCC 3 cut(s) 50, 163, 195
ApoI RAATTY 1 cut(s) 171
AspLEI GCGC 2 cut(s) 368, 391
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 213, 227
AvaII GGWCC 1 cut(s) 148
AxyI CCTNAGG 1 cut(s) 54
BalI TGGCCA 1 cut(s) 165
BanI GGYRCC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 118
BccI CCATC 3 cut(s) 222, 267, 391
BceAI ACGGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 369
BfuI GTATCC 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 315
BmrI ACTGGG 1 cut(s) 137
BmuI ACTGGG 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 366
BsaJI CCNNGG 2 cut(s) 47, 166
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse1I ACTGG 2 cut(s) 29, 143
Bse21I CCTNAGG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 435
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 47, 166
BseGI GGATG 2 cut(s) 305, 436
BseJI GATNNNNATC 1 cut(s) 435
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 3 cut(s) 68, 183, 213
BseNI ACTGG 2 cut(s) 29, 143
BseRI GAGGAG 1 cut(s) 36
BshFI GGCC 3 cut(s) 52, 165, 197
BshNI GGYRCC 1 cut(s) 365
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 35
BsnI GGCC 3 cut(s) 52, 165, 197
Bsp1286I GDGCHC 1 cut(s) 118
Bsp19I CCATGG 1 cut(s) 166
BspANI GGCC 3 cut(s) 52, 165, 197
BspCNI CTCAG 3 cut(s) 67, 184, 212
BspLI GGNNCC 1 cut(s) 367
BspT107I GGYRCC 1 cut(s) 365
BsrI ACTGG 2 cut(s) 29, 143
BssECI CCNNGG 2 cut(s) 47, 166
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 166
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 134, 494
BstACI GRCGYC 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 3 cut(s) 54, 192, 199
BstDSI CCRYGG 1 cut(s) 166
BstEII GGTNACC 1 cut(s) 446
BstF5I GGATG 2 cut(s) 305, 436
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 2 cut(s) 368, 391
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 313
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 3 cut(s) 52, 165, 197
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 305, 436
BtsIMutI CAGTG 3 cut(s) 36, 130, 150
Cac8I GCNNGC 1 cut(s) 103
CfoI GCGC 2 cut(s) 368, 391
Cfr13I GGNCC 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 2 cut(s) 98, 167
CviJI RGCY 7 cut(s) 28, 52, 165, 197, 354, 481, 487
CviKI_1 RGCY 7 cut(s) 28, 52, 165, 197, 354, 481, 487
DdeI CTNAG 3 cut(s) 54, 192, 199
DinI GGCGCC 1 cut(s) 367
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 93
Eco81I CCTNAGG 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 166
EgeI GGCGCC 1 cut(s) 367
EheI GGCGCC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 166
FaeI CATG 2 cut(s) 101, 170
FaiI YATR 7 cut(s) 10, 99, 168, 226, 428, 436, 518
FatI CATG 2 cut(s) 97, 166
FokI GGATG 2 cut(s) 292, 443
FspI TGCGCA 1 cut(s) 390
GlaI GCGC 2 cut(s) 367, 390
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 3 cut(s) 52, 165, 197
HapII CCGG 1 cut(s) 363
HhaI GCGC 2 cut(s) 368, 391
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 101, 170
Hin6I GCGC 2 cut(s) 366, 389
HinP1I GCGC 2 cut(s) 366, 389
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 1 cut(s) 138
HpaII CCGG 1 cut(s) 363
HphI GGTGA 2 cut(s) 213, 227
Hpy166II GTNNAC 4 cut(s) 137, 148, 415, 508
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 4 cut(s) 137, 148, 415, 508
HpyAV CCTTC 2 cut(s) 28, 79
HpyCH4III ACNGT 2 cut(s) 134, 494
HpyCH4V TGCA 2 cut(s) 62, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 3 cut(s) 54, 192, 199
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 101, 170
HspAI GCGC 2 cut(s) 366, 389
KasI GGCGCC 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 446
MboII GAAGA 3 cut(s) 16, 86, 395
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 1 cut(s) 118
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 3 cut(s) 171, 187, 498
MluNI TGGCCA 1 cut(s) 165
Mly113I GGCGCC 1 cut(s) 366
MlyI GAGTC 1 cut(s) 132
MnlI CCTC 8 cut(s) 54, 57, 58, 63, 187, 208, 248, 283
Mox20I TGGCCA 1 cut(s) 165
MscI TGGCCA 1 cut(s) 165
MseI TTAA 2 cut(s) 122, 474
MslI CAYNNNNRTG 1 cut(s) 159
Msp20I TGGCCA 1 cut(s) 165
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 315
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 395
NarI GGCGCC 1 cut(s) 366
NcoI CCATGG 1 cut(s) 166
NlaIII CATG 2 cut(s) 101, 170
NlaIV GGNNCC 1 cut(s) 367
NmeAIII GCCGAG 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 390
PceI AGGCCT 1 cut(s) 197
PflMI CCANNNNNTGG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 313
PleI GAGTC 1 cut(s) 132
PluTI GGCGCC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 446
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 1 cut(s) 148
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 122, 474
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 118
SetI ASST 7 cut(s) 20, 60, 240, 356, 483, 513, 527
SfoI GGCGCC 1 cut(s) 367
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 3 cut(s) 171, 187, 498
SseBI AGGCCT 1 cut(s) 197
SspDI GGCGCC 1 cut(s) 365
StuI AGGCCT 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 134, 494
TasI AATT 3 cut(s) 171, 187, 498
Tru1I TTAA 2 cut(s) 122, 474
Tru9I TTAA 2 cut(s) 122, 474
TscAI CASTG 3 cut(s) 36, 137, 150
TspDTI ATGAA 2 cut(s) 17, 323
TspRI CASTG 3 cut(s) 36, 137, 150
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.