Rh2DG055300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000544
Physical Location & Seq
Reverse (-)
4015421 .. 4022454
7034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG055300.1

Sequence Viewer

Length: 819 bp
ATGGCGGTGATGGCAGATTTTGGTCTGCTTATTCTGTTCTTCATTGCGTTGTGGGACGCTGAGGCGGTGTTGAGTTCAGGGGTTGCAGGTGTTGTTGTTTTTGCGCCCAAGCTCGACGGTGACATGAATGATGCGGCCTCCGGGAGGGAGTTGGCATCTAAATGGCAACTGAGCAGCGATCCAGTCTTGGTTTGGTCAAGATCGGATCGGAAAGGTCGATTCCGATCGAGTCCATGGGAAGATGTTCTCTGGCGTGCAGGCGATCTTGATCTCCCGGACGGTGACAGAGCGCGGGTTTGCTCGGACGCAGGGACAATTGCTTCTACTGGTGGTGCTCATCGGTATATCGACGGTCAGTACGGAGAAACCGGGTCCTGGAAGGATCGGGACGACTTCTTGTCGAGGTTGAGCGAGGCAGGACTCACAATGAAACAGTTCAACGCATTATACTTCCACCGGCTCTTACAAAACAGAGATCAGAGGGCAGCGTTGCCTGTTCAATTCATTGACAGGCTGCGGCATCGCTCTGTGAGTTATAATGGATGTTTACAGCCCTGGGAGAAAGCTTACACCGATTGGGAGCGTGCTAACTATAAGGAGAATATAGACCTGCATAAGGCATGCTCGGTGGATCCATGGGCTTTAAAAGTGTACAAGTTCAACACTTACAGGGACGATCCAGCTAACCGTGTAAAGCTTGCAAGGAATATCATGATTCATCTTAATGATTACAGGGACCGCGAACATAAGGGAACAGATGGTAGTAAAACCACCAAAAGTAGCATAGTCTGTGGTGATTGTGAAGGAAATGGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

30.73

Weight (kDa)

7.0

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 2 cut(s) 77, 618
AccII CGCG 2 cut(s) 292, 741
AciI CCGC 6 cut(s) 5, 65, 134, 292, 517, 739
AclWI GGATC 6 cut(s) 173, 213, 390, 626, 639, 671
AfaI GTAC 2 cut(s) 359, 653
AfiI CCNNNNNNNGG 3 cut(s) 144, 375, 616
AgsI TTSAA 3 cut(s) 439, 500, 661
AhdI GACNNNNNGTC 1 cut(s) 397
AjnI CCWGG 2 cut(s) 374, 554
AluBI AGCT 4 cut(s) 112, 566, 683, 697
AluI AGCT 4 cut(s) 112, 566, 683, 697
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 6 cut(s) 173, 213, 390, 626, 639, 671
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 3 cut(s) 174, 485, 514
Asp700I GAANNNNTTC 2 cut(s) 243, 434
AspLEI GCGC 2 cut(s) 106, 292
AspS9I GGNCC 2 cut(s) 372, 736
AsuC2I CCSGG 3 cut(s) 142, 275, 370
AsuHPI GGTGA 4 cut(s) 19, 131, 293, 806
AvaII GGWCC 2 cut(s) 372, 736
BamHI GGATCC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 186, 497, 501
BccI CCATC 2 cut(s) 4, 752
BciT130I CCWGG 2 cut(s) 376, 556
BcnI CCSGG 3 cut(s) 142, 275, 370
BfuAI ACCTGC 2 cut(s) 77, 618
BisI GCNGC 5 cut(s) 135, 175, 486, 515, 518
BlsI GCNGC 5 cut(s) 136, 176, 487, 516, 519
Bme1390I CCNGG 5 cut(s) 142, 275, 370, 376, 556
Bme18I GGWCC 2 cut(s) 372, 736
BmeRI GACNNNNNGTC 1 cut(s) 397
BmgT120I GGNCC 2 cut(s) 372, 736
BmiI GGNNCC 3 cut(s) 373, 633, 737
BmrFI CCNGG 5 cut(s) 142, 275, 370, 376, 556
BmsI GCATC 3 cut(s) 121, 164, 529
Bpu10I CCTNAGC 1 cut(s) 60
BpuMI CCSGG 3 cut(s) 142, 275, 370
BsaBI GATNNNNATC 2 cut(s) 223, 267
BsaJI CCNNGG 4 cut(s) 233, 554, 555, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 375, 616
Bse118I RCCGGY 1 cut(s) 456
Bse1I ACTGG 2 cut(s) 182, 331
Bse3DI GCAATG 1 cut(s) 42
Bse8I GATNNNNATC 2 cut(s) 223, 267
BseBI CCWGG 2 cut(s) 376, 556
BseDI CCNNGG 4 cut(s) 233, 554, 555, 635
BseGI GGATG 1 cut(s) 548
BseJI GATNNNNATC 2 cut(s) 223, 267
BseLI CCNNNNNNNGG 3 cut(s) 144, 375, 616
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 51, 161
BseNI ACTGG 2 cut(s) 182, 331
BseXI GCAGC 3 cut(s) 186, 497, 501
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 2 cut(s) 292, 741
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 4 cut(s) 141, 275, 369, 457
BslFI GGGAC 5 cut(s) 68, 325, 401, 686, 749
BslI CCNNNNNNNGG 3 cut(s) 144, 375, 616
BsmFI GGGAC 5 cut(s) 68, 325, 401, 686, 749
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 337
Bsp1407I TGTACA 1 cut(s) 651
Bsp19I CCATGG 2 cut(s) 233, 635
BspACI CCGC 6 cut(s) 5, 65, 134, 292, 517, 739
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 2 cut(s) 52, 162
BspFNI CGCG 2 cut(s) 292, 741
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 3 cut(s) 373, 633, 737
BspMI ACCTGC 2 cut(s) 77, 618
BspPI GGATC 6 cut(s) 173, 213, 390, 626, 639, 671
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 651
BsrI ACTGG 2 cut(s) 182, 331
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 4 cut(s) 233, 554, 555, 635
BssT1I CCWWGG 2 cut(s) 233, 635
Bst2UI CCWGG 2 cut(s) 376, 556
Bst4CI ACNGT 5 cut(s) 119, 281, 353, 435, 689
BstAUI TGTACA 1 cut(s) 651
BstC8I GCNNGC 5 cut(s) 255, 259, 585, 622, 699
BstDEI CTNAG 2 cut(s) 60, 170
BstDSI CCRYGG 2 cut(s) 233, 635
BstENI CCTNNNNNAGG 2 cut(s) 142, 614
BstF5I GGATG 1 cut(s) 548
BstFNI CGCG 2 cut(s) 292, 741
BstHHI GCGC 2 cut(s) 106, 292
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 376, 556
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 5 cut(s) 140, 273, 368, 374, 554
BstUI CGCG 2 cut(s) 292, 741
BstV1I GCAGC 3 cut(s) 186, 497, 501
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 2 cut(s) 233, 635
BtgZI GCGATG 1 cut(s) 506
BtsCI GGATG 1 cut(s) 548
BveI ACCTGC 2 cut(s) 77, 618
Cac8I GCNNGC 5 cut(s) 255, 259, 585, 622, 699
CciI TCATGA 1 cut(s) 711
CfoI GCGC 2 cut(s) 106, 292
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 372, 736
CseI GACGC 2 cut(s) 65, 314
Csp6I GTAC 2 cut(s) 358, 652
CviAII CATG 5 cut(s) 124, 234, 621, 636, 712
CviJI RGCY 9 cut(s) 112, 137, 460, 514, 553, 566, 641, 683, 697
CviKI_1 RGCY 9 cut(s) 112, 137, 460, 514, 553, 566, 641, 683, 697
CviQI GTAC 2 cut(s) 358, 652
DdeI CTNAG 2 cut(s) 60, 170
DraI TTTAAA 1 cut(s) 645
DriI GACNNNNNGTC 1 cut(s) 397
Eam1105I GACNNNNNGTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 233, 635
Eco47I GGWCC 2 cut(s) 372, 736
EcoNI CCTNNNNNAGG 2 cut(s) 142, 614
EcoO109I RGGNCCY 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 374, 554
EcoT14I CCWWGG 2 cut(s) 233, 635
ErhI CCWWGG 2 cut(s) 233, 635
FaeI CATG 5 cut(s) 127, 237, 624, 639, 715
FaqI GGGAC 5 cut(s) 68, 325, 401, 686, 749
FatI CATG 5 cut(s) 123, 233, 620, 635, 711
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 5 cut(s) 135, 175, 486, 515, 518
FokI GGATG 1 cut(s) 555
Fsp4HI GCNGC 5 cut(s) 135, 175, 486, 515, 518
GlaI GCGC 2 cut(s) 105, 291
GluI GCNGC 5 cut(s) 135, 175, 486, 515, 518
HaeIII GGCC 1 cut(s) 137
HapII CCGG 4 cut(s) 141, 275, 369, 457
HgaI GACGC 2 cut(s) 65, 314
HhaI GCGC 2 cut(s) 106, 292
Hin1II CATG 5 cut(s) 127, 237, 624, 639, 715
Hin6I GCGC 2 cut(s) 104, 290
HinP1I GCGC 2 cut(s) 104, 290
HindIII AAGCTT 2 cut(s) 564, 695
HinfI GANTC 4 cut(s) 219, 229, 420, 715
HpaII CCGG 4 cut(s) 141, 275, 369, 457
HphI GGTGA 4 cut(s) 19, 131, 293, 806
Hpy166II GTNNAC 2 cut(s) 548, 652
Hpy188I TCNGA 5 cut(s) 205, 210, 224, 304, 480
Hpy188III TCNNGA 4 cut(s) 198, 266, 386, 712
Hpy8I GTNNAC 2 cut(s) 548, 652
Hpy99I CGWCG 2 cut(s) 119, 353
HpyAV CCTTC 2 cut(s) 373, 797
HpyCH4III ACNGT 5 cut(s) 119, 281, 353, 435, 689
HpyCH4V TGCA 4 cut(s) 86, 257, 613, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 60, 170
Hsp92II CATG 5 cut(s) 127, 237, 624, 639, 715
HspAI GCGC 2 cut(s) 104, 290
LmnI GCTCC 1 cut(s) 580
Lsp1109I GCAGC 3 cut(s) 186, 497, 501
LweI GCATC 3 cut(s) 121, 164, 529
MaeIII GTNAC 2 cut(s) 119, 281
MboII GAAGA 2 cut(s) 31, 251
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 2 cut(s) 315, 500
MlyI GAGTC 2 cut(s) 238, 414
MnlI CCTC 6 cut(s) 55, 138, 148, 396, 406, 474
MroXI GAANNNNTTC 2 cut(s) 243, 434
MseI TTAA 2 cut(s) 644, 723
MslI CAYNNNNRTG 2 cut(s) 160, 723
MspI CCGG 4 cut(s) 141, 275, 369, 457
MspR9I CCNGG 5 cut(s) 142, 275, 370, 376, 556
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 2 cut(s) 376, 556
MvnI CGCG 2 cut(s) 292, 741
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 3 cut(s) 142, 275, 370
NcoI CCATGG 2 cut(s) 233, 635
NlaIII CATG 5 cut(s) 127, 237, 624, 639, 715
NlaIV GGNNCC 3 cut(s) 373, 633, 737
NmuCI GTSAC 2 cut(s) 119, 281
NspI RCATGY 1 cut(s) 624
PaeI GCATGC 1 cut(s) 624
PagI TCATGA 1 cut(s) 711
PaqCI CACCTGC 1 cut(s) 77
PasI CCCWGGG 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 243, 434
PfeI GAWTC 2 cut(s) 219, 715
PfoI TCCNGGA 3 cut(s) 140, 273, 374
PkrI GCNGC 5 cut(s) 136, 176, 487, 516, 519
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 2 cut(s) 237, 414
PpsI GAGTC 2 cut(s) 237, 414
PpuMI RGGWCCY 1 cut(s) 372
PsiI TTATAA 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 374, 554
PspGI CCWGG 2 cut(s) 374, 554
PspN4I GGNNCC 3 cut(s) 373, 633, 737
PspPI GGNCC 2 cut(s) 372, 736
PspPPI RGGWCCY 1 cut(s) 372
PsuI RGATCY 1 cut(s) 631
PvuI CGATCG 1 cut(s) 227
RsaI GTAC 2 cut(s) 359, 653
RsaNI GTAC 2 cut(s) 358, 652
RseI CAYNNNNRTG 2 cut(s) 160, 723
SaqAI TTAA 2 cut(s) 644, 723
SatI GCNGC 5 cut(s) 135, 175, 486, 515, 518
Sau96I GGNCC 2 cut(s) 372, 736
SchI GAGTC 2 cut(s) 238, 414
ScrFI CCNGG 5 cut(s) 142, 275, 370, 376, 556
SduI GDGCHC 1 cut(s) 337
SetI ASST 8 cut(s) 91, 114, 217, 407, 568, 612, 685, 699
SfaNI GCATC 3 cut(s) 121, 164, 529
SinI GGWCC 2 cut(s) 372, 736
SmiMI CAYNNNNRTG 2 cut(s) 160, 723
SphI GCATGC 1 cut(s) 624
Sse9I AATT 2 cut(s) 315, 500
SsiI CCGC 6 cut(s) 5, 65, 134, 292, 517, 739
StyD4I CCNGG 5 cut(s) 140, 273, 368, 374, 554
StyI CCWWGG 2 cut(s) 233, 635
TaaI ACNGT 5 cut(s) 119, 281, 353, 435, 689
TaqI TCGA 5 cut(s) 114, 217, 227, 348, 401
TasI AATT 2 cut(s) 315, 500
TatI WGTACW 1 cut(s) 651
TauI GCSGC 2 cut(s) 137, 520
TfiI GAWTC 2 cut(s) 219, 715
Tru1I TTAA 2 cut(s) 644, 723
Tru9I TTAA 2 cut(s) 644, 723
TseFI GTSAC 2 cut(s) 119, 281
TseI GCWGC 3 cut(s) 174, 485, 514
Tsp45I GTSAC 2 cut(s) 119, 281
TspDTI ATGAA 5 cut(s) 31, 140, 443, 493, 707
TspGWI ACGGA 1 cut(s) 375
VpaK11BI GGWCC 2 cut(s) 372, 736
XagI CCTNNNNNAGG 2 cut(s) 142, 614
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XmnI GAANNNNTTC 2 cut(s) 243, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.