Rmu_sc0000259.1_g000018

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000259.1
OG0000544
Physical Location & Seq
Reverse (-)
81926 .. 83622
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000259.1_g000018.1.cds

Sequence Viewer

Length: 885 bp
atggccgagggacgttctgagcgaaggcgtgccagaggcgaatgtatccaggatacgatcgatcgcttgggatggatattctatgaggtcaccaactggaagaatgaattgctatggtatcaatcggcgcctatcctccaaaagttgaatagggatgtgttagatgaagtggcatttagatgggctaacaatatggctgtacctcccttggatacatatgctgagaaagtcagggataacaaagcctatattgcgagacaaggctctcgaaacgattcttggaactacacaattcggtatcgggatttcattctttctgtttatggccctgtggcagataataggcttcttgatgatttttgtagaaggctgagtgacccaaccctcacactgctcggatttcagaaattgtatttccataaacttttacaaagccgggaggaaatcactgacttcccagtaatggcggttgaaaacctccggcttaggaagtatctttacaacgagacagagtgcaattgggatgtggcatatcgattgtgggcagaggagaaataccacaaggagattaatctttctgaaacttgcctgaaaaacatttatgcttggagagcctacaattacagaatttacactgataatcccacagatgaagccgagctagtgcgtaatctcatcatccacttcaaccagtgcaggatgaagaatgtccccccaccagcagatttctatgataaacatcaaattggagaagtggtattcgacctctttcctgatattggttccagcctctttgatttcatgtgcgaatgtggagtttatatggacttcacgcagttcgtacattttacagagaataataagcctgaacctaatttattgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

35.18

Weight (kDa)

5.41

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 127
AciI CCGC 1 cut(s) 467
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 627
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 2 cut(s) 201, 843
AfiI CCNNNNNNNGG 2 cut(s) 463, 779
AgsI TTSAA 3 cut(s) 148, 473, 688
AjnI CCWGG 1 cut(s) 48
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 1 cut(s) 661
AluI AGCT 1 cut(s) 661
Alw26I GTCTC 2 cut(s) 250, 500
AoxI GGCC 2 cut(s) 3, 325
ApoI RAATTY 1 cut(s) 627
AseI ATTAAT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 274
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 1 cut(s) 82
BanI GGYRCC 1 cut(s) 127
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BccI CCATC 2 cut(s) 66, 174
BciT130I CCWGG 1 cut(s) 50
BciVI GTATCC 3 cut(s) 46, 56, 205
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 250, 500
BfaI CTAG 1 cut(s) 662
BfoI RGCGCY 1 cut(s) 131
BfuI GTATCC 3 cut(s) 46, 56, 205
Bme1390I CCNGG 2 cut(s) 50, 437
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 129, 784
BmrFI CCNGG 2 cut(s) 50, 437
BmrI ACTGGG 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 452
Bpu10I CCTNAGC 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 2 cut(s) 60, 535
BsaBI GATNNNNATC 1 cut(s) 738
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 6, 207
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 779
Bse1I ACTGG 3 cut(s) 101, 458, 691
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 1 cut(s) 50
BseCI ATCGAT 2 cut(s) 60, 535
BseDI CCNNGG 2 cut(s) 6, 207
BseGI GGATG 5 cut(s) 77, 160, 529, 678, 705
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 2 cut(s) 463, 779
BseMII CTCAG 3 cut(s) 9, 213, 362
BseNI ACTGG 3 cut(s) 101, 458, 691
BseRI GAGGAG 1 cut(s) 563
BsgI GTGCAG 1 cut(s) 715
Bsh1285I CGRYCG 2 cut(s) 60, 64
BshFI GGCC 2 cut(s) 5, 327
BshNI GGYRCC 1 cut(s) 127
BshVI ATCGAT 2 cut(s) 60, 535
BsiEI CGRYCG 2 cut(s) 60, 64
BsiSI CCGG 2 cut(s) 436, 481
BslFI GGGAC 2 cut(s) 24, 695
BslI CCNNNNNNNGG 2 cut(s) 463, 779
BsmAI GTCTC 2 cut(s) 250, 500
BsmFI GGGAC 2 cut(s) 24, 695
BsnI GGCC 2 cut(s) 5, 327
Bsp143I GATC 2 cut(s) 57, 61
BspACI CCGC 1 cut(s) 467
BspANI GGCC 2 cut(s) 5, 327
BspCNI CTCAG 3 cut(s) 10, 214, 363
BspDI ATCGAT 2 cut(s) 60, 535
BspLI GGNNCC 2 cut(s) 129, 784
BspT107I GGYRCC 1 cut(s) 127
BsrI ACTGG 3 cut(s) 101, 458, 691
BssECI CCNNGG 2 cut(s) 6, 207
BssMI GATC 2 cut(s) 57, 61
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 4 cut(s) 18, 222, 371, 485
BstEII GGTNACC 1 cut(s) 88
BstF5I GGATG 5 cut(s) 77, 160, 529, 678, 705
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 2 cut(s) 60, 64
BstMAI GTCTC 2 cut(s) 250, 500
BstMBI GATC 2 cut(s) 57, 61
BstMCI CGRYCG 2 cut(s) 60, 64
BstMWI GCNNNNNNNGC 2 cut(s) 251, 611
BstNI CCWGG 1 cut(s) 50
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 48, 435
Bsu15I ATCGAT 2 cut(s) 60, 535
BsuI GTATCC 3 cut(s) 46, 56, 205
BsuRI GGCC 2 cut(s) 5, 327
BsuTUI ATCGAT 2 cut(s) 60, 535
BtsCI GGATG 5 cut(s) 77, 160, 529, 678, 705
BtsI GCAGTG 1 cut(s) 389
BtsIMutI CAGTG 4 cut(s) 389, 447, 633, 698
Cac8I GCNNGC 1 cut(s) 30
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 326
ClaI ATCGAT 2 cut(s) 60, 535
Csp6I GTAC 2 cut(s) 200, 842
CviAII CATG 1 cut(s) 802
CviQI GTAC 2 cut(s) 200, 842
DdeI CTNAG 4 cut(s) 18, 222, 371, 485
DinI GGCGCC 1 cut(s) 129
DpnI GATC 2 cut(s) 59, 63
DpnII GATC 2 cut(s) 57, 61
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 88
EcoO65I GGTNACC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 207
EgeI GGCGCC 1 cut(s) 129
EheI GGCGCC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 805
FaqI GGGAC 2 cut(s) 24, 695
FatI CATG 1 cut(s) 801
FauNDI CATATG 1 cut(s) 217
FokI GGATG 5 cut(s) 84, 167, 536, 665, 712
FspBI CTAG 1 cut(s) 662
GlaI GCGC 1 cut(s) 129
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 5, 327
HapII CCGG 2 cut(s) 436, 481
HhaI GCGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 1 cut(s) 805
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 275
HpaII CCGG 2 cut(s) 436, 481
HphI GGTGA 1 cut(s) 82
Hpy188I TCNGA 4 cut(s) 19, 398, 405, 580
Hpy188III TCNNGA 4 cut(s) 267, 302, 350, 773
HpyAV CCTTC 2 cut(s) 18, 360
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 2 cut(s) 516, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 611
HpyF3I CTNAG 4 cut(s) 18, 222, 371, 485
HpySE526I ACGT 1 cut(s) 13
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 1 cut(s) 805
HspAI GCGC 1 cut(s) 128
KasI GGCGCC 1 cut(s) 127
Kzo9I GATC 2 cut(s) 57, 61
MaeI CTAG 1 cut(s) 662
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 2 cut(s) 88, 374
MalI GATC 2 cut(s) 59, 63
MboI GATC 2 cut(s) 57, 61
MboII GAAGA 2 cut(s) 112, 715
MfeI CAATTG 1 cut(s) 517
MluCI AATT 8 cut(s) 107, 291, 407, 517, 619, 627, 744, 874
Mly113I GGCGCC 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 274
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 178
MspI CCGG 2 cut(s) 436, 481
MspR9I CCNGG 2 cut(s) 50, 437
MunI CAATTG 1 cut(s) 517
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 251, 611
NarI GGCGCC 1 cut(s) 128
NciI CCSGG 1 cut(s) 437
NdeI CATATG 1 cut(s) 217
NdeII GATC 2 cut(s) 57, 61
NlaIII CATG 1 cut(s) 805
NlaIV GGNNCC 2 cut(s) 129, 784
NmeAIII GCCGAG 2 cut(s) 31, 682
NmuCI GTSAC 2 cut(s) 88, 374
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 48
Ple19I CGATCG 2 cut(s) 60, 64
PluTI GGCGCC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 48
PspEI GGTNACC 1 cut(s) 88
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 129, 784
PspPI GGNCC 1 cut(s) 326
PvuI CGATCG 2 cut(s) 60, 64
RsaI GTAC 2 cut(s) 201, 843
RsaNI GTAC 2 cut(s) 200, 842
RseI CAYNNNNRTG 1 cut(s) 178
SaqAI TTAA 1 cut(s) 570
Sau3AI GATC 2 cut(s) 57, 61
Sau96I GGNCC 1 cut(s) 326
ScrFI CCNGG 2 cut(s) 50, 437
SetI ASST 7 cut(s) 16, 90, 205, 480, 663, 768, 874
SfoI GGCGCC 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 178
Sse9I AATT 8 cut(s) 107, 291, 407, 517, 619, 627, 744, 874
SsiI CCGC 1 cut(s) 467
SspDI GGCGCC 1 cut(s) 127
SspMI CTAG 1 cut(s) 662
StyD4I CCNGG 2 cut(s) 48, 435
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 16
TaqI TCGA 4 cut(s) 60, 268, 535, 762
TasI AATT 8 cut(s) 107, 291, 407, 517, 619, 627, 744, 874
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 4 cut(s) 396, 454, 640, 698
TseFI GTSAC 2 cut(s) 88, 374
Tsp45I GTSAC 2 cut(s) 88, 374
TspDTI ATGAA 6 cut(s) 120, 180, 298, 666, 716, 790
TspRI CASTG 4 cut(s) 396, 454, 640, 698
VspI ATTAAT 1 cut(s) 570
XapI RAATTY 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 274
XspI CTAG 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.