The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 487 |
| AcoI | YGGCCR | 1 cut(s) | 519 |
| AfaI | GTAC | 2 cut(s) | 23, 396 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 106, 188, 200, 258, 340 |
| AgsI | TTSAA | 2 cut(s) | 142, 302 |
| AluBI | AGCT | 3 cut(s) | 115, 211, 328 |
| AluI | AGCT | 3 cut(s) | 115, 211, 328 |
| AlwI | GGATC | 1 cut(s) | 487 |
| AoxI | GGCC | 2 cut(s) | 435, 519 |
| ApeKI | GCWGC | 2 cut(s) | 112, 328 |
| AspLEI | GCGC | 2 cut(s) | 112, 333 |
| AspS9I | GGNCC | 4 cut(s) | 118, 191, 322, 435 |
| AsuC2I | CCSGG | 1 cut(s) | 227 |
| AsuNHI | GCTAGC | 1 cut(s) | 404 |
| AvaII | GGWCC | 3 cut(s) | 118, 191, 322 |
| BbsI | GAAGAC | 1 cut(s) | 246 |
| BbvI | GCAGC | 2 cut(s) | 124, 315 |
| BccI | CCATC | 1 cut(s) | 494 |
| BceAI | ACGGC | 1 cut(s) | 506 |
| BcnI | CCSGG | 1 cut(s) | 227 |
| BfaI | CTAG | 1 cut(s) | 405 |
| BfmI | CTRYAG | 1 cut(s) | 605 |
| BisI | GCNGC | 2 cut(s) | 113, 329 |
| BlsI | GCNGC | 2 cut(s) | 114, 330 |
| Bme1390I | CCNGG | 1 cut(s) | 227 |
| Bme18I | GGWCC | 3 cut(s) | 118, 191, 322 |
| BmgT120I | GGNCC | 4 cut(s) | 118, 191, 322, 435 |
| BmrFI | CCNGG | 1 cut(s) | 227 |
| BmrI | ACTGGG | 1 cut(s) | 442 |
| BmsI | GCATC | 3 cut(s) | 55, 569, 588 |
| BmtI | GCTAGC | 1 cut(s) | 408 |
| BmuI | ACTGGG | 1 cut(s) | 442 |
| BpiI | GAAGAC | 1 cut(s) | 246 |
| BpuEI | CTTGAG | 1 cut(s) | 540 |
| BpuMI | CCSGG | 1 cut(s) | 227 |
| BsaBI | GATNNNNATC | 1 cut(s) | 549 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 106, 188, 200, 258, 340 |
| Bse1I | ACTGG | 4 cut(s) | 39, 398, 437, 586 |
| Bse8I | GATNNNNATC | 1 cut(s) | 549 |
| BseJI | GATNNNNATC | 1 cut(s) | 549 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 106, 188, 200, 258, 340 |
| BseNI | ACTGG | 4 cut(s) | 39, 398, 437, 586 |
| BseXI | GCAGC | 2 cut(s) | 124, 315 |
| BsgI | GTGCAG | 1 cut(s) | 157 |
| BshFI | GGCC | 2 cut(s) | 437, 521 |
| BsiSI | CCGG | 1 cut(s) | 226 |
| BslFI | GGGAC | 1 cut(s) | 30 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 106, 188, 200, 258, 340 |
| BsmFI | GGGAC | 1 cut(s) | 30 |
| BsnI | GGCC | 2 cut(s) | 437, 521 |
| Bsp143I | GATC | 3 cut(s) | 3, 479, 544 |
| BspANI | GGCC | 2 cut(s) | 437, 521 |
| BspOI | GCTAGC | 1 cut(s) | 408 |
| BspPI | GGATC | 1 cut(s) | 487 |
| BsrI | ACTGG | 4 cut(s) | 39, 398, 437, 586 |
| BssMI | GATC | 3 cut(s) | 3, 479, 544 |
| Bst4CI | ACNGT | 4 cut(s) | 21, 460, 542, 606 |
| BstC8I | GCNNGC | 1 cut(s) | 406 |
| BstDEI | CTNAG | 2 cut(s) | 177, 263 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 104, 338 |
| BstHHI | GCGC | 2 cut(s) | 112, 333 |
| BstKTI | GATC | 3 cut(s) | 6, 482, 547 |
| BstMBI | GATC | 3 cut(s) | 3, 479, 544 |
| BstSCI | CCNGG | 1 cut(s) | 225 |
| BstSFI | CTRYAG | 1 cut(s) | 605 |
| BstV1I | GCAGC | 2 cut(s) | 124, 315 |
| BstV2I | GAAGAC | 1 cut(s) | 246 |
| BsuRI | GGCC | 2 cut(s) | 437, 521 |
| BtsI | GCAGTG | 2 cut(s) | 93, 352 |
| BtsIMutI | CAGTG | 3 cut(s) | 93, 352, 602 |
| Cac8I | GCNNGC | 1 cut(s) | 406 |
| CfoI | GCGC | 2 cut(s) | 112, 333 |
| Cfr13I | GGNCC | 4 cut(s) | 118, 191, 322, 435 |
| Csp6I | GTAC | 2 cut(s) | 22, 395 |
| CviAII | CATG | 1 cut(s) | 409 |
| CviJI | RGCY | 7 cut(s) | 115, 181, 211, 262, 328, 437, 521 |
| CviKI_1 | RGCY | 7 cut(s) | 115, 181, 211, 262, 328, 437, 521 |
| CviQI | GTAC | 2 cut(s) | 22, 395 |
| DdeI | CTNAG | 2 cut(s) | 177, 263 |
| DpnI | GATC | 3 cut(s) | 5, 481, 546 |
| DpnII | GATC | 3 cut(s) | 3, 479, 544 |
| EaeI | YGGCCR | 1 cut(s) | 519 |
| Eco32I | GATATC | 1 cut(s) | 223 |
| Eco47I | GGWCC | 3 cut(s) | 118, 191, 322 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 104, 338 |
| EcoRV | GATATC | 1 cut(s) | 223 |
| EcoT22I | ATGCAT | 1 cut(s) | 70 |
| FaeI | CATG | 1 cut(s) | 412 |
| FaiI | YATR | 5 cut(s) | 70, 198, 410, 444, 566 |
| FaqI | GGGAC | 1 cut(s) | 30 |
| FatI | CATG | 1 cut(s) | 408 |
| Fnu4HI | GCNGC | 2 cut(s) | 113, 329 |
| Fsp4HI | GCNGC | 2 cut(s) | 113, 329 |
| FspBI | CTAG | 1 cut(s) | 405 |
| GlaI | GCGC | 2 cut(s) | 111, 332 |
| GluI | GCNGC | 2 cut(s) | 113, 329 |
| HaeIII | GGCC | 2 cut(s) | 437, 521 |
| HapII | CCGG | 1 cut(s) | 226 |
| HhaI | GCGC | 2 cut(s) | 112, 333 |
| Hin1II | CATG | 1 cut(s) | 412 |
| Hin6I | GCGC | 2 cut(s) | 110, 331 |
| HinP1I | GCGC | 2 cut(s) | 110, 331 |
| HincII | GTYRAC | 1 cut(s) | 389 |
| HindII | GTYRAC | 1 cut(s) | 389 |
| HinfI | GANTC | 1 cut(s) | 575 |
| HpaII | CCGG | 1 cut(s) | 226 |
| Hpy166II | GTNNAC | 2 cut(s) | 194, 389 |
| Hpy188I | TCNGA | 5 cut(s) | 45, 80, 122, 322, 364 |
| Hpy8I | GTNNAC | 2 cut(s) | 194, 389 |
| HpyAV | CCTTC | 1 cut(s) | 520 |
| HpyCH4III | ACNGT | 4 cut(s) | 21, 460, 542, 606 |
| HpyCH4IV | ACGT | 2 cut(s) | 186, 255 |
| HpyCH4V | TGCA | 3 cut(s) | 68, 174, 269 |
| HpyF3I | CTNAG | 2 cut(s) | 177, 263 |
| HpySE526I | ACGT | 2 cut(s) | 186, 255 |
| Hsp92II | CATG | 1 cut(s) | 412 |
| HspAI | GCGC | 2 cut(s) | 110, 331 |
| Kzo9I | GATC | 3 cut(s) | 3, 479, 544 |
| LpnPI | CCDG | 9 cut(s) | 52, 101, 136, 239, 303, 338, 411, 418, 599 |
| Lsp1109I | GCAGC | 2 cut(s) | 124, 315 |
| LweI | GCATC | 3 cut(s) | 55, 569, 588 |
| MaeI | CTAG | 1 cut(s) | 405 |
| MaeII | ACGT | 2 cut(s) | 186, 255 |
| MaeIII | GTNAC | 1 cut(s) | 34 |
| MalI | GATC | 3 cut(s) | 5, 481, 546 |
| MboI | GATC | 3 cut(s) | 3, 479, 544 |
| MboII | GAAGA | 2 cut(s) | 58, 251 |
| MluCI | AATT | 1 cut(s) | 25 |
| MnlI | CCTC | 4 cut(s) | 6, 100, 344, 564 |
| Mph1103I | ATGCAT | 1 cut(s) | 70 |
| MseI | TTAA | 3 cut(s) | 159, 282, 581 |
| MslI | CAYNNNNRTG | 1 cut(s) | 302 |
| MspA1I | CMGCKG | 2 cut(s) | 115, 328 |
| MspI | CCGG | 1 cut(s) | 226 |
| MspR9I | CCNGG | 1 cut(s) | 227 |
| NciI | CCSGG | 1 cut(s) | 227 |
| NdeII | GATC | 3 cut(s) | 3, 479, 544 |
| NheI | GCTAGC | 1 cut(s) | 404 |
| NlaIII | CATG | 1 cut(s) | 412 |
| NsiI | ATGCAT | 1 cut(s) | 70 |
| PfeI | GAWTC | 1 cut(s) | 575 |
| PfoI | TCCNGGA | 1 cut(s) | 225 |
| PkrI | GCNGC | 2 cut(s) | 114, 330 |
| PspPI | GGNCC | 4 cut(s) | 118, 191, 322, 435 |
| PvuII | CAGCTG | 2 cut(s) | 115, 328 |
| RsaI | GTAC | 2 cut(s) | 23, 396 |
| RsaNI | GTAC | 2 cut(s) | 22, 395 |
| RseI | CAYNNNNRTG | 1 cut(s) | 302 |
| SaqAI | TTAA | 3 cut(s) | 159, 282, 581 |
| SatI | GCNGC | 2 cut(s) | 113, 329 |
| Sau3AI | GATC | 3 cut(s) | 3, 479, 544 |
| Sau96I | GGNCC | 4 cut(s) | 118, 191, 322, 435 |
| ScrFI | CCNGG | 1 cut(s) | 227 |
| SetI | ASST | 7 cut(s) | 86, 117, 189, 213, 258, 330, 361 |
| SfaNI | GCATC | 3 cut(s) | 55, 569, 588 |
| SfcI | CTRYAG | 1 cut(s) | 605 |
| SinI | GGWCC | 3 cut(s) | 118, 191, 322 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 302 |
| SmlI | CTYRAG | 1 cut(s) | 555 |
| SmoI | CTYRAG | 1 cut(s) | 555 |
| Sse9I | AATT | 1 cut(s) | 25 |
| SspMI | CTAG | 1 cut(s) | 405 |
| StyD4I | CCNGG | 1 cut(s) | 225 |
| TaaI | ACNGT | 4 cut(s) | 21, 460, 542, 606 |
| TaiI | ACGT | 2 cut(s) | 189, 258 |
| TaqI | TCGA | 4 cut(s) | 6, 133, 237, 308 |
| TasI | AATT | 1 cut(s) | 25 |
| TatI | WGTACW | 1 cut(s) | 21 |
| TfiI | GAWTC | 1 cut(s) | 575 |
| Tru1I | TTAA | 3 cut(s) | 159, 282, 581 |
| Tru9I | TTAA | 3 cut(s) | 159, 282, 581 |
| TscAI | CASTG | 3 cut(s) | 100, 352, 609 |
| TseI | GCWGC | 2 cut(s) | 112, 328 |
| TspDTI | ATGAA | 2 cut(s) | 462, 588 |
| TspRI | CASTG | 3 cut(s) | 100, 352, 609 |
| VpaK11BI | GGWCC | 3 cut(s) | 118, 191, 322 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 104, 338 |
| XspI | CTAG | 1 cut(s) | 405 |
| Zsp2I | ATGCAT | 1 cut(s) | 70 |