RLG00000016217

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000544
Physical Location & Seq
Reverse (-)
4800718 .. 4802381
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016217

Sequence Viewer

Length: 855 bp
ATGACGAATGGCAAGCGGAAGCCCAGCAAAGCTACTGGTCCCGTGGCGGTGGCAAAGCGTCAGAGGTTACCGAATCGTTCGACGGTGGATGCGTACCTCCGATACCGTGGAGTGGTCGTCAAACGTAGAGTCTTCAGCGTCTTCGATAAGAAGAAAGTCTTGGACTGGATTCCTGAGGAAATCTTTCAACATCTAACCAGAGATTTGATAAACAGGGTAGATTATCGTTGGGGGCATGATTTGCCAATTGGATCCTTAGACCCTGCCCATTTAAGGGTTGAAGATAATGCCCTGGTTCTTCCTGCGGTTGGCTCTGGTCCCGAGGATAGGGATTACGCTGGCAGAACTAGAAGCTTTGTTGTTCGAGTCTTTGGGGGAACGGGGTCCTGGAAGGATTTGGATGACTTCCTGTCCAGATTAAGCATCCCAGGGCTCAGAAGGGAACAGTTCACTGCTTTGTACTTCCACAGGCTGTTGCAGAACAAAGATCAGCGGGCAGTGGTGCCTGTTGAATTTGTCGATAAGATGCGCAACCGTTTAGTGACTTATAATGGAACTGTAAAGCGCTGGGAAGCAGCTTACAATGAATGGGAGCAAACAGTCTATCCGGAGAAAATAGATTTAGAACAGGCTTGCAGTAAAGATCCCGGGGCTCTAAAACTTGCCCGCAACATAATCATACATTTGAACGACTATCGGATTGGCAAGAAGCAATCTATTTACACTCCATACGAGCTTGGCGCTCTGGTGGATGAGCTATTTCCCAACCTCAGCTCTAGATTGTTCGAGTTTCTATGGAAGCAACGCATCAACCTGGACTTCAAGAGGTCCAGGAACACTTTCAAATCCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

33.1

Weight (kDa)

9.91

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
Acc16I TGCGCA 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 502
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 5 cut(s) 16, 47, 305, 493, 667
AclWI GGATC 3 cut(s) 246, 259, 638
AcsI RAATTY 1 cut(s) 512
AcuI CTGAAG 1 cut(s) 118
AfaI GTAC 2 cut(s) 95, 461
AfeI AGCGCT 1 cut(s) 566
AfiI CCNNNNNNNGG 5 cut(s) 112, 273, 274, 308, 327
AgsI TTSAA 6 cut(s) 188, 281, 512, 688, 823, 844
AhdI GACNNNNNGTC 1 cut(s) 409
AjnI CCWGG 5 cut(s) 291, 386, 427, 813, 830
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 6 cut(s) 32, 354, 578, 736, 757, 774
AluI AGCT 6 cut(s) 32, 354, 578, 736, 757, 774
AlwI GGATC 3 cut(s) 246, 259, 638
Ama87I CYCGRG 2 cut(s) 320, 647
Aor13HI TCCGGA 1 cut(s) 607
Aor51HI AGCGCT 1 cut(s) 566
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 2 cut(s) 183, 839
AspLEI GCGC 3 cut(s) 531, 567, 743
AspS9I GGNCC 4 cut(s) 38, 317, 384, 828
AsuC2I CCSGG 2 cut(s) 648, 649
AvaI CYCGRG 2 cut(s) 320, 647
AvaII GGWCC 4 cut(s) 38, 317, 384, 828
AxyI CCTNAGG 1 cut(s) 174
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 502
BanII GRGCYC 2 cut(s) 435, 655
BbsI GAAGAC 2 cut(s) 124, 133
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 1 cut(s) 587
BciT130I CCWGG 5 cut(s) 293, 388, 429, 815, 832
BcnI CCSGG 2 cut(s) 648, 649
BfaI CTAG 2 cut(s) 348, 777
BfoI RGCGCY 2 cut(s) 568, 744
BisI GCNGC 1 cut(s) 576
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 7 cut(s) 293, 388, 429, 648, 649, 815, 832
Bme18I GGWCC 4 cut(s) 38, 317, 384, 828
BmeRI GACNNNNNGTC 1 cut(s) 409
BmeT110I CYCGRG 2 cut(s) 320, 647
BmgT120I GGNCC 4 cut(s) 38, 317, 384, 828
BmiI GGNNCC 5 cut(s) 40, 253, 319, 385, 504
BmrFI CCNGG 7 cut(s) 293, 388, 429, 648, 649, 815, 832
BmsI GCATC 4 cut(s) 79, 432, 516, 816
BpiI GAAGAC 2 cut(s) 124, 133
Bpu10I CCTNAGC 1 cut(s) 770
BpuMI CCSGG 2 cut(s) 648, 649
BsaJI CCNNGG 8 cut(s) 42, 106, 291, 321, 427, 428, 647, 648
BsaWI WCCGGW 1 cut(s) 607
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 5 cut(s) 112, 273, 274, 308, 327
Bse1I ACTGG 2 cut(s) 40, 170
Bse21I CCTNAGG 1 cut(s) 174
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 5 cut(s) 293, 388, 429, 815, 832
BseDI CCNNGG 8 cut(s) 42, 106, 291, 321, 427, 428, 647, 648
BseGI GGATG 4 cut(s) 94, 406, 423, 757
BseLI CCNNNNNNNGG 5 cut(s) 112, 273, 274, 308, 327
BseMII CTCAG 3 cut(s) 165, 448, 784
BseNI ACTGG 2 cut(s) 40, 170
BseXI GCAGC 1 cut(s) 587
BseYI CCCAGC 2 cut(s) 23, 567
BshNI GGYRCC 1 cut(s) 502
BsiHKCI CYCGRG 2 cut(s) 320, 647
BsiSI CCGG 2 cut(s) 608, 648
BslFI GGGAC 2 cut(s) 24, 303
BslI CCNNNNNNNGG 5 cut(s) 112, 273, 274, 308, 327
BsmFI GGGAC 2 cut(s) 24, 303
BsoBI CYCGRG 2 cut(s) 320, 647
Bsp1286I GDGCHC 2 cut(s) 435, 655
Bsp13I TCCGGA 1 cut(s) 607
Bsp143I GATC 3 cut(s) 251, 487, 643
BspACI CCGC 5 cut(s) 16, 47, 305, 493, 667
BspCNI CTCAG 3 cut(s) 166, 447, 783
BspEI TCCGGA 1 cut(s) 607
BspLI GGNNCC 5 cut(s) 40, 253, 319, 385, 504
BspPI GGATC 3 cut(s) 246, 259, 638
BspT107I GGYRCC 1 cut(s) 502
BsrI ACTGG 2 cut(s) 40, 170
BssECI CCNNGG 8 cut(s) 42, 106, 291, 321, 427, 428, 647, 648
BssMI GATC 3 cut(s) 251, 487, 643
Bst2UI CCWGG 5 cut(s) 293, 388, 429, 815, 832
Bst4CI ACNGT 6 cut(s) 85, 107, 447, 536, 559, 601
BstAPI GCANNNNNTGC 1 cut(s) 241
BstC8I GCNNGC 5 cut(s) 14, 340, 495, 634, 667
BstDEI CTNAG 4 cut(s) 174, 256, 434, 770
BstDSI CCRYGG 2 cut(s) 42, 106
BstEII GGTNACC 1 cut(s) 66
BstF5I GGATG 4 cut(s) 94, 406, 423, 757
BstH2I RGCGCY 2 cut(s) 568, 744
BstHHI GCGC 3 cut(s) 531, 567, 743
BstKTI GATC 3 cut(s) 254, 490, 646
BstMBI GATC 3 cut(s) 251, 487, 643
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 5 cut(s) 293, 388, 429, 815, 832
BstPI GGTNACC 1 cut(s) 66
BstSCI CCNGG 7 cut(s) 291, 386, 427, 646, 647, 813, 830
BstV1I GCAGC 1 cut(s) 587
BstV2I GAAGAC 2 cut(s) 124, 133
BstX2I RGATCY 2 cut(s) 251, 643
BstYI RGATCY 2 cut(s) 251, 643
Bsu36I CCTNAGG 1 cut(s) 174
BtgI CCRYGG 2 cut(s) 42, 106
BtsCI GGATG 4 cut(s) 94, 406, 423, 757
BtsI GCAGTG 2 cut(s) 450, 504
BtsIMutI CAGTG 2 cut(s) 450, 504
Cac8I GCNNGC 5 cut(s) 14, 340, 495, 634, 667
CfoI GCGC 3 cut(s) 531, 567, 743
Cfr13I GGNCC 4 cut(s) 38, 317, 384, 828
Cfr9I CCCGGG 1 cut(s) 647
CseI GACGC 2 cut(s) 47, 127
Csp6I GTAC 2 cut(s) 94, 460
CviAII CATG 1 cut(s) 236
CviQI GTAC 2 cut(s) 94, 460
DdeI CTNAG 4 cut(s) 174, 256, 434, 770
DpnI GATC 3 cut(s) 253, 489, 645
DpnII GATC 3 cut(s) 251, 487, 643
DriI GACNNNNNGTC 1 cut(s) 409
Eam1105I GACNNNNNGTC 1 cut(s) 409
Eco24I GRGCYC 2 cut(s) 435, 655
Eco47I GGWCC 4 cut(s) 38, 317, 384, 828
Eco47III AGCGCT 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 174
Eco88I CYCGRG 2 cut(s) 320, 647
Eco91I GGTNACC 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 384
EcoO65I GGTNACC 1 cut(s) 66
EcoRII CCWGG 5 cut(s) 291, 386, 427, 813, 830
EcoT38I GRGCYC 2 cut(s) 435, 655
FaeI CATG 1 cut(s) 239
FaiI YATR 8 cut(s) 237, 549, 674, 680, 730, 796, 851, 853
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FaqI GGGAC 2 cut(s) 24, 303
FatI CATG 1 cut(s) 235
FauI CCCGC 2 cut(s) 486, 674
FauNDI CATATG 1 cut(s) 851
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 4 cut(s) 101, 410, 413, 764
FriOI GRGCYC 2 cut(s) 435, 655
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 2 cut(s) 348, 777
FspI TGCGCA 1 cut(s) 530
GlaI GCGC 3 cut(s) 530, 566, 742
GluI GCNGC 1 cut(s) 576
GsaI CCCAGC 2 cut(s) 27, 571
HaeII RGCGCY 2 cut(s) 568, 744
HapII CCGG 2 cut(s) 608, 648
HgaI GACGC 2 cut(s) 47, 127
HhaI GCGC 3 cut(s) 531, 567, 743
Hin1II CATG 1 cut(s) 239
Hin6I GCGC 3 cut(s) 529, 565, 741
HinP1I GCGC 3 cut(s) 529, 565, 741
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 4 cut(s) 73, 129, 169, 366
HpaII CCGG 2 cut(s) 608, 648
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 4 cut(s) 63, 101, 437, 699
Hpy188III TCNNGA 6 cut(s) 173, 320, 414, 608, 777, 823
Hpy8I GTNNAC 1 cut(s) 450
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 2 cut(s) 385, 432
HpyCH4III ACNGT 6 cut(s) 85, 107, 447, 536, 559, 601
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 478, 636
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 4 cut(s) 174, 256, 434, 770
HpySE526I ACGT 1 cut(s) 124
Hsp92II CATG 1 cut(s) 239
HspAI GCGC 3 cut(s) 529, 565, 741
Kpn2I TCCGGA 1 cut(s) 607
Kzo9I GATC 3 cut(s) 251, 487, 643
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 4 cut(s) 79, 432, 516, 816
MaeI CTAG 2 cut(s) 348, 777
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 66, 541
MalI GATC 3 cut(s) 253, 489, 645
MboI GATC 3 cut(s) 251, 487, 643
MboII GAAGA 5 cut(s) 124, 133, 163, 290, 293
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 2 cut(s) 251, 643
MhlI GDGCHC 2 cut(s) 435, 655
MluCI AATT 2 cut(s) 246, 512
MlyI GAGTC 2 cut(s) 138, 375
MnlI CCTC 6 cut(s) 57, 107, 169, 316, 779, 819
MroI TCCGGA 1 cut(s) 607
MroXI GAANNNNTTC 2 cut(s) 183, 839
MseI TTAA 2 cut(s) 272, 419
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 2 cut(s) 608, 648
MspR9I CCNGG 7 cut(s) 293, 388, 429, 648, 649, 815, 832
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 5 cut(s) 293, 388, 429, 815, 832
MwoI GCNNNNNNNGC 1 cut(s) 241
NciI CCSGG 2 cut(s) 648, 649
NdeI CATATG 1 cut(s) 851
NdeII GATC 3 cut(s) 251, 487, 643
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 5 cut(s) 40, 253, 319, 385, 504
NmuCI GTSAC 1 cut(s) 541
NsbI TGCGCA 1 cut(s) 530
PasI CCCWGGG 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 183, 839
PfeI GAWTC 2 cut(s) 73, 169
PfoI TCCNGGA 2 cut(s) 386, 830
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 2 cut(s) 137, 374
PpsI GAGTC 2 cut(s) 137, 374
PpuMI RGGWCCY 1 cut(s) 384
PsiI TTATAA 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 5 cut(s) 291, 386, 427, 813, 830
PspEI GGTNACC 1 cut(s) 66
PspFI CCCAGC 2 cut(s) 23, 567
PspGI CCWGG 5 cut(s) 291, 386, 427, 813, 830
PspN4I GGNNCC 5 cut(s) 40, 253, 319, 385, 504
PspPI GGNCC 4 cut(s) 38, 317, 384, 828
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 2 cut(s) 251, 643
RsaI GTAC 2 cut(s) 95, 461
RsaNI GTAC 2 cut(s) 94, 460
SaqAI TTAA 2 cut(s) 272, 419
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 3 cut(s) 251, 487, 643
Sau96I GGNCC 4 cut(s) 38, 317, 384, 828
SchI GAGTC 2 cut(s) 138, 375
ScrFI CCNGG 7 cut(s) 293, 388, 429, 648, 649, 815, 832
SduI GDGCHC 2 cut(s) 435, 655
SfaNI GCATC 4 cut(s) 79, 432, 516, 816
SinI GGWCC 4 cut(s) 38, 317, 384, 828
SmaI CCCGGG 1 cut(s) 649
Sse9I AATT 2 cut(s) 246, 512
SsiI CCGC 5 cut(s) 16, 47, 305, 493, 667
SspMI CTAG 2 cut(s) 348, 777
StyD4I CCNGG 7 cut(s) 291, 386, 427, 646, 647, 813, 830
TaaI ACNGT 6 cut(s) 85, 107, 447, 536, 559, 601
TaiI ACGT 1 cut(s) 127
TaqI TCGA 5 cut(s) 80, 144, 364, 519, 786
TasI AATT 2 cut(s) 246, 512
TatI WGTACW 1 cut(s) 459
TfiI GAWTC 2 cut(s) 73, 169
Tru1I TTAA 2 cut(s) 272, 419
Tru9I TTAA 2 cut(s) 272, 419
TscAI CASTG 2 cut(s) 457, 504
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 600
TspMI CCCGGG 1 cut(s) 647
TspRI CASTG 2 cut(s) 457, 504
VpaK11BI GGWCC 4 cut(s) 38, 317, 384, 828
XapI RAATTY 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 776
XmaI CCCGGG 1 cut(s) 647
XmnI GAANNNNTTC 2 cut(s) 183, 839
XspI CTAG 2 cut(s) 348, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.