The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| TPR_COPA_B | PF23953 | 2 - 138 | 1.3e-44 | COPA/B TPR domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 44 |
| AcuI | CTGAAG | 1 cut(s) | 192 |
| AfaI | GTAC | 1 cut(s) | 387 |
| AflIII | ACRYGT | 1 cut(s) | 29 |
| AgsI | TTSAA | 3 cut(s) | 260, 359, 413 |
| AjnI | CCWGG | 1 cut(s) | 428 |
| AluBI | AGCT | 6 cut(s) | 64, 130, 143, 256, 331, 365 |
| AluI | AGCT | 6 cut(s) | 64, 130, 143, 256, 331, 365 |
| ApeKI | GCWGC | 2 cut(s) | 127, 362 |
| ApoI | RAATTY | 1 cut(s) | 44 |
| AspLEI | GCGC | 1 cut(s) | 106 |
| BbvI | GCAGC | 2 cut(s) | 139, 374 |
| BccI | CCATC | 2 cut(s) | 187, 364 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 74, 108 |
| BciT130I | CCWGG | 1 cut(s) | 430 |
| BfaI | CTAG | 2 cut(s) | 65, 338 |
| BisI | GCNGC | 2 cut(s) | 128, 363 |
| BlsI | GCNGC | 2 cut(s) | 129, 364 |
| Bme1390I | CCNGG | 1 cut(s) | 430 |
| BmrFI | CCNGG | 1 cut(s) | 430 |
| BmsI | GCATC | 2 cut(s) | 226, 333 |
| BpmI | CTGGAG | 1 cut(s) | 95 |
| BpuEI | CTTGAG | 1 cut(s) | 225 |
| BsaAI | YACGTR | 1 cut(s) | 385 |
| BsaJI | CCNNGG | 2 cut(s) | 84, 289 |
| BsaWI | WCCGGW | 1 cut(s) | 16 |
| Bse1I | ACTGG | 1 cut(s) | 448 |
| BseBI | CCWGG | 1 cut(s) | 430 |
| BseDI | CCNNGG | 2 cut(s) | 84, 289 |
| BseGI | GGATG | 3 cut(s) | 16, 198, 324 |
| BseNI | ACTGG | 1 cut(s) | 448 |
| BseXI | GCAGC | 2 cut(s) | 139, 374 |
| BseYI | CCCAGC | 3 cut(s) | 130, 304, 389 |
| BsiSI | CCGG | 1 cut(s) | 17 |
| BsmI | GAATGC | 1 cut(s) | 284 |
| Bsp143I | GATC | 2 cut(s) | 49, 377 |
| BspQI | GCTCTTC | 1 cut(s) | 255 |
| BsrI | ACTGG | 1 cut(s) | 448 |
| BssECI | CCNNGG | 2 cut(s) | 84, 289 |
| BssMI | GATC | 2 cut(s) | 49, 377 |
| BssT1I | CCWWGG | 1 cut(s) | 289 |
| Bst2UI | CCWGG | 1 cut(s) | 430 |
| Bst4CI | ACNGT | 1 cut(s) | 110 |
| Bst6I | CTCTTC | 1 cut(s) | 255 |
| BstBAI | YACGTR | 1 cut(s) | 385 |
| BstF5I | GGATG | 3 cut(s) | 16, 198, 324 |
| BstHHI | GCGC | 1 cut(s) | 106 |
| BstKTI | GATC | 2 cut(s) | 52, 380 |
| BstMBI | GATC | 2 cut(s) | 49, 377 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 101, 262, 371 |
| BstNI | CCWGG | 1 cut(s) | 430 |
| BstNSI | RCATGY | 1 cut(s) | 33 |
| BstSCI | CCNGG | 1 cut(s) | 428 |
| BstSNI | TACGTA | 1 cut(s) | 385 |
| BstV1I | GCAGC | 2 cut(s) | 139, 374 |
| BtsCI | GGATG | 3 cut(s) | 16, 198, 324 |
| BtsIMutI | CAGTG | 1 cut(s) | 115 |
| CfoI | GCGC | 1 cut(s) | 106 |
| Csp6I | GTAC | 1 cut(s) | 386 |
| CviAII | CATG | 3 cut(s) | 30, 301, 394 |
| CviJI | RGCY | 6 cut(s) | 64, 130, 143, 256, 331, 365 |
| CviKI_1 | RGCY | 6 cut(s) | 64, 130, 143, 256, 331, 365 |
| CviQI | GTAC | 1 cut(s) | 386 |
| DpnI | GATC | 2 cut(s) | 51, 379 |
| DpnII | GATC | 2 cut(s) | 49, 377 |
| Eam1104I | CTCTTC | 1 cut(s) | 255 |
| EarI | CTCTTC | 1 cut(s) | 255 |
| Eco105I | TACGTA | 1 cut(s) | 385 |
| Eco130I | CCWWGG | 1 cut(s) | 289 |
| Eco57I | CTGAAG | 1 cut(s) | 192 |
| EcoRII | CCWGG | 1 cut(s) | 428 |
| EcoT14I | CCWWGG | 1 cut(s) | 289 |
| ErhI | CCWWGG | 1 cut(s) | 289 |
| FaeI | CATG | 3 cut(s) | 33, 304, 397 |
| FaiI | YATR | 8 cut(s) | 31, 59, 146, 182, 302, 395, 478, 484 |
| FatI | CATG | 3 cut(s) | 29, 300, 393 |
| Fnu4HI | GCNGC | 2 cut(s) | 128, 363 |
| FokI | GGATG | 3 cut(s) | 23, 205, 311 |
| Fsp4HI | GCNGC | 2 cut(s) | 128, 363 |
| FspBI | CTAG | 2 cut(s) | 65, 338 |
| GlaI | GCGC | 1 cut(s) | 105 |
| GluI | GCNGC | 2 cut(s) | 128, 363 |
| GsaI | CCCAGC | 3 cut(s) | 134, 308, 393 |
| GsuI | CTGGAG | 1 cut(s) | 95 |
| HapII | CCGG | 1 cut(s) | 17 |
| HhaI | GCGC | 1 cut(s) | 106 |
| Hin1II | CATG | 3 cut(s) | 33, 304, 397 |
| Hin6I | GCGC | 1 cut(s) | 104 |
| HinP1I | GCGC | 1 cut(s) | 104 |
| HindIII | AAGCTT | 1 cut(s) | 254 |
| HinfI | GANTC | 1 cut(s) | 113 |
| HpaII | CCGG | 1 cut(s) | 17 |
| Hpy188I | TCNGA | 3 cut(s) | 37, 205, 403 |
| Hpy188III | TCNNGA | 1 cut(s) | 74 |
| HpyCH4III | ACNGT | 1 cut(s) | 110 |
| HpyCH4IV | ACGT | 1 cut(s) | 384 |
| HpyCH4V | TGCA | 2 cut(s) | 14, 284 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 101, 262, 371 |
| HpySE526I | ACGT | 1 cut(s) | 384 |
| Hsp92II | CATG | 3 cut(s) | 33, 304, 397 |
| HspAI | GCGC | 1 cut(s) | 104 |
| Kzo9I | GATC | 2 cut(s) | 49, 377 |
| LguI | GCTCTTC | 1 cut(s) | 255 |
| Lsp1109I | GCAGC | 2 cut(s) | 139, 374 |
| LweI | GCATC | 2 cut(s) | 226, 333 |
| MaeI | CTAG | 2 cut(s) | 65, 338 |
| MaeII | ACGT | 1 cut(s) | 384 |
| MaeIII | GTNAC | 2 cut(s) | 210, 308 |
| MalI | GATC | 2 cut(s) | 51, 379 |
| MboI | GATC | 2 cut(s) | 49, 377 |
| MboII | GAAGA | 5 cut(s) | 185, 216, 272, 329, 460 |
| MluCI | AATT | 3 cut(s) | 44, 137, 413 |
| MnlI | CCTC | 2 cut(s) | 79, 160 |
| MspA1I | CMGCKG | 2 cut(s) | 130, 331 |
| MspI | CCGG | 1 cut(s) | 17 |
| MspR9I | CCNGG | 1 cut(s) | 430 |
| Mva1269I | GAATGC | 1 cut(s) | 284 |
| MvaI | CCWGG | 1 cut(s) | 430 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 101, 262, 371 |
| NdeII | GATC | 2 cut(s) | 49, 377 |
| NlaIII | CATG | 3 cut(s) | 33, 304, 397 |
| NmeAIII | GCCGAG | 1 cut(s) | 109 |
| NspI | RCATGY | 1 cut(s) | 33 |
| PciI | ACATGT | 1 cut(s) | 29 |
| PciSI | GCTCTTC | 1 cut(s) | 255 |
| PctI | GAATGC | 1 cut(s) | 284 |
| PfeI | GAWTC | 1 cut(s) | 113 |
| PflFI | GACNNNGTC | 1 cut(s) | 436 |
| PkrI | GCNGC | 2 cut(s) | 129, 364 |
| Ppu21I | YACGTR | 1 cut(s) | 385 |
| PscI | ACATGT | 1 cut(s) | 29 |
| Psp6I | CCWGG | 1 cut(s) | 428 |
| PspFI | CCCAGC | 3 cut(s) | 130, 304, 389 |
| PspGI | CCWGG | 1 cut(s) | 428 |
| PsyI | GACNNNGTC | 1 cut(s) | 436 |
| PvuII | CAGCTG | 2 cut(s) | 130, 331 |
| RsaI | GTAC | 1 cut(s) | 387 |
| RsaNI | GTAC | 1 cut(s) | 386 |
| SapI | GCTCTTC | 1 cut(s) | 255 |
| SatI | GCNGC | 2 cut(s) | 128, 363 |
| Sau3AI | GATC | 2 cut(s) | 49, 377 |
| ScrFI | CCNGG | 1 cut(s) | 430 |
| SetI | ASST | 9 cut(s) | 66, 70, 132, 145, 258, 333, 367, 387, 431 |
| SfaNI | GCATC | 2 cut(s) | 226, 333 |
| SmlI | CTYRAG | 1 cut(s) | 240 |
| SmoI | CTYRAG | 1 cut(s) | 240 |
| SnaBI | TACGTA | 1 cut(s) | 385 |
| Sse9I | AATT | 3 cut(s) | 44, 137, 413 |
| SspMI | CTAG | 2 cut(s) | 65, 338 |
| StyD4I | CCNGG | 1 cut(s) | 428 |
| StyI | CCWWGG | 1 cut(s) | 289 |
| TaaI | ACNGT | 1 cut(s) | 110 |
| TaiI | ACGT | 1 cut(s) | 387 |
| TasI | AATT | 3 cut(s) | 44, 137, 413 |
| TfiI | GAWTC | 1 cut(s) | 113 |
| TscAI | CASTG | 1 cut(s) | 115 |
| TseI | GCWGC | 2 cut(s) | 127, 362 |
| TspDTI | ATGAA | 1 cut(s) | 289 |
| TspRI | CASTG | 1 cut(s) | 115 |
| Tth111I | GACNNNGTC | 1 cut(s) | 436 |
| XapI | RAATTY | 1 cut(s) | 44 |
| XceI | RCATGY | 1 cut(s) | 33 |
| XspI | CTAG | 2 cut(s) | 65, 338 |