Rroxscaffold_4G00332590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000544
Physical Location & Seq
Forward (+)
66784220 .. 66786242
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332590.1

Sequence Viewer

Length: 1002 bp
ATGGCCAAGAAACGTTCTGAGCGTAAGAGGAAGAGCAAGAAGCACAAGGGGTCTGAGCGCAAAAGGAAGGAGAGGAGGTCTGAGCGGAAGAAAAAGTCTGATCGAAGGAGAAGGCGTGCCAGAGACGAATGTATCCAGGATACGATCGATCGCTTGGGATGGATATTCTATAAGGTCACCAACTGGAAGAATGAATTGTTATGGTATCAATCGGCGCCTATCCTCCAGAAGTTGAGTAGGGATGTGTTAGATGAAGTGGCATTTAGATGGGCTAACAAGATGGGTGTGCCTCCCTTGAATACTTATAGTGTGAAAGTCAGGGACAACAAAGGCTATATTCCGAGGCAAGGCTCTCGAGATGATTCTAGGAACTACGCACATCGGTATCTGTATTTCATCCTTTCTGTCTATGGCCCTGTGGCTGATAATAGGCTTCTTGATGACTTTTGTAGAAAGCTGAGTCAACCAAACCTCACACTGCTCAGATTTCAGAATTTGTATTTCCATAAACTTTTACAAAGCCGAGAGGAAATCAATGACTTCCCAGTAGTGGCGGTTGAAAACCTCCGGCTTAGGAAGTATTTTTACAATGGGAGAGTGCGCAATTGGGATGTGGCATATCAATTGTGGGCAGAGGAGAAATACCACAGGGAGATTAATCTTTTTGAAACTTGCATAAAAAACATTTATGTGTGGAGAGCCTACAATTACAGAATTTACGCTGATAATCCCACAGATGAAGCCGAGCTAGTGCGTAATCTCATCATCCACTTCAACCAGTGCAGGATGAAGAATGTTCTCCCACCTGCAGATTTCTATGATAAAAATCAGATTGGAGAAGTGGTATTCGACCTCTTTCCTGATATTGATTCCAGCCTCTTTGATTTCATGTACGAACGTGGAGTTTATATGGACTTCACAGCACTTCGTACATTTTACAGGTGCCACTTTGCGTTTTTGATTAGTTTCCTTGAGGCAATATTTCATGCTCTTGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

333

Amino Acids

40.44

Weight (kDa)

9.71

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 814
Acc16I TGCGCA 1 cut(s) 602
Acc36I ACCTGC 1 cut(s) 814
AccB1I GGYRCC 2 cut(s) 214, 942
AccBSI CCGCTC 1 cut(s) 85
AciI CCGC 2 cut(s) 85, 554
AclI AACGTT 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 493, 714
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 2 cut(s) 893, 931
AfiI CCNNNNNNNGG 2 cut(s) 347, 550
AgsI TTSAA 4 cut(s) 298, 560, 668, 775
AjnI CCWGG 1 cut(s) 135
AluBI AGCT 2 cut(s) 457, 748
AluI AGCT 2 cut(s) 457, 748
Alw26I GTCTC 1 cut(s) 117
Ama87I CYCGRG 1 cut(s) 354
AoxI GGCC 2 cut(s) 3, 412
ApoI RAATTY 2 cut(s) 493, 714
AseI ATTAAT 1 cut(s) 657
AspLEI GCGC 3 cut(s) 60, 217, 603
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 354
BaeI ACNNNNGTAYC 4 cut(s) 115, 148, 368, 401
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 214, 942
BarI GAAGNNNNNNTAC 2 cut(s) 569, 601
BccI CCATC 3 cut(s) 153, 261, 274
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 1 cut(s) 137
BciVI GTATCC 2 cut(s) 133, 143
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 366, 749
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 814
BfuI GTATCC 2 cut(s) 133, 143
Bme1390I CCNGG 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 216, 944
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 539
BmuI ACTGGG 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 992
Bsa29I ATCGAT 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 825
BsaHI GRCGYC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 341
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 100, 130, 828, 858
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 550
Bse1I ACTGG 3 cut(s) 188, 545, 778
Bse8I GATNNNNATC 1 cut(s) 825
BseBI CCWGG 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 341
BseGI GGATG 6 cut(s) 164, 247, 396, 616, 765, 792
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 2 cut(s) 347, 550
BseMII CTCAG 5 cut(s) 9, 45, 72, 449, 496
BseNI ACTGG 3 cut(s) 188, 545, 778
BseRI GAGGAG 2 cut(s) 88, 650
BsgI GTGCAG 1 cut(s) 802
Bsh1285I CGRYCG 2 cut(s) 147, 151
BshFI GGCC 2 cut(s) 5, 414
BshNI GGYRCC 2 cut(s) 214, 942
BshVI ATCGAT 1 cut(s) 147
BsiEI CGRYCG 2 cut(s) 147, 151
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 1 cut(s) 568
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 347, 550
BsmAI GTCTC 1 cut(s) 117
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 2 cut(s) 5, 414
BsoBI CYCGRG 1 cut(s) 354
Bsp143I GATC 3 cut(s) 100, 144, 148
BspACI CCGC 2 cut(s) 85, 554
BspANI GGCC 2 cut(s) 5, 414
BspCNI CTCAG 5 cut(s) 10, 46, 73, 450, 495
BspDI ATCGAT 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 216, 944
BspMAI CTGCAG 1 cut(s) 811
BspMI ACCTGC 1 cut(s) 814
BspQI GCTCTTC 1 cut(s) 26
BspT107I GGYRCC 2 cut(s) 214, 942
BsrBI CCGCTC 1 cut(s) 85
BsrI ACTGG 3 cut(s) 188, 545, 778
BssECI CCNNGG 1 cut(s) 341
BssMI GATC 3 cut(s) 100, 144, 148
BssNI GRCGYC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 137
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 7 cut(s) 18, 54, 81, 458, 482, 572, 999
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 6 cut(s) 164, 247, 396, 616, 765, 792
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 3 cut(s) 60, 217, 603
BstKTI GATC 3 cut(s) 103, 147, 151
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 3 cut(s) 100, 144, 148
BstMCI CGRYCG 2 cut(s) 147, 151
BstMWI GCNNNNNNNGC 1 cut(s) 995
BstNI CCWGG 1 cut(s) 137
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 807
Bsu15I ATCGAT 1 cut(s) 147
BsuI GTATCC 2 cut(s) 133, 143
BsuRI GGCC 2 cut(s) 5, 414
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 6 cut(s) 164, 247, 396, 616, 765, 792
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 2 cut(s) 476, 785
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 1 cut(s) 117
CfoI GCGC 3 cut(s) 60, 217, 603
Cfr13I GGNCC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 147
Csp6I GTAC 2 cut(s) 892, 930
CviAII CATG 2 cut(s) 889, 986
CviQI GTAC 2 cut(s) 892, 930
DdeI CTNAG 7 cut(s) 18, 54, 81, 458, 482, 572, 999
DinI GGCGCC 1 cut(s) 216
DpnI GATC 3 cut(s) 102, 146, 150
DpnII GATC 3 cut(s) 100, 144, 148
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 135
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 2 cut(s) 892, 989
FaqI GGGAC 1 cut(s) 335
FatI CATG 2 cut(s) 888, 985
FokI GGATG 6 cut(s) 171, 254, 383, 623, 752, 799
FspBI CTAG 2 cut(s) 366, 749
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 3 cut(s) 59, 216, 602
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 2 cut(s) 5, 414
HapII CCGG 1 cut(s) 568
HhaI GCGC 3 cut(s) 60, 217, 603
Hin1I GRCGYC 1 cut(s) 215
Hin1II CATG 2 cut(s) 892, 989
Hin6I GCGC 3 cut(s) 58, 215, 601
HinP1I GCGC 3 cut(s) 58, 215, 601
HincII GTYRAC 1 cut(s) 464
HindII GTYRAC 1 cut(s) 464
HinfI GANTC 3 cut(s) 362, 460, 869
HpaII CCGG 1 cut(s) 568
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 8 cut(s) 19, 55, 82, 100, 342, 485, 492, 831
Hpy188III TCNNGA 5 cut(s) 226, 354, 356, 437, 860
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 3 cut(s) 61, 99, 105
HpyCH4IV ACGT 2 cut(s) 13, 898
HpyCH4V TGCA 3 cut(s) 675, 783, 809
HpyF10VI GCNNNNNNNGC 1 cut(s) 995
HpyF3I CTNAG 7 cut(s) 18, 54, 81, 458, 482, 572, 999
HpySE526I ACGT 2 cut(s) 13, 898
Hsp92I GRCGYC 1 cut(s) 215
Hsp92II CATG 2 cut(s) 892, 989
HspAI GCGC 3 cut(s) 58, 215, 601
KasI GGCGCC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 100, 144, 148
LguI GCTCTTC 1 cut(s) 26
MaeI CTAG 2 cut(s) 366, 749
MaeII ACGT 2 cut(s) 13, 898
MaeIII GTNAC 1 cut(s) 175
MalI GATC 3 cut(s) 102, 146, 150
MbiI CCGCTC 1 cut(s) 85
MboI GATC 3 cut(s) 100, 144, 148
MboII GAAGA 4 cut(s) 43, 100, 199, 802
MfeI CAATTG 2 cut(s) 604, 623
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 194, 493, 604, 623, 706, 714
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 1 cut(s) 469
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 657
MslI CAYNNNNRTG 2 cut(s) 265, 689
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 137
MunI CAATTG 2 cut(s) 604, 623
MvaI CCWGG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 995
NarI GGCGCC 1 cut(s) 215
NdeII GATC 3 cut(s) 100, 144, 148
NlaIII CATG 2 cut(s) 892, 989
NlaIV GGNNCC 2 cut(s) 216, 944
NmeAIII GCCGAG 2 cut(s) 548, 769
NmuCI GTSAC 1 cut(s) 175
NsbI TGCGCA 1 cut(s) 602
PaeR7I CTCGAG 1 cut(s) 354
PaqCI CACCTGC 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PfeI GAWTC 2 cut(s) 362, 869
PfoI TCCNGGA 1 cut(s) 135
Ple19I CGATCG 2 cut(s) 147, 151
PleI GAGTC 1 cut(s) 468
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 468
PshBI ATTAAT 1 cut(s) 657
Psp1406I AACGTT 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 135
PspEI GGTNACC 1 cut(s) 175
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 216, 944
PspPI GGNCC 1 cut(s) 413
PstI CTGCAG 1 cut(s) 811
PvuI CGATCG 2 cut(s) 147, 151
RsaI GTAC 2 cut(s) 893, 931
RsaNI GTAC 2 cut(s) 892, 930
RseI CAYNNNNRTG 2 cut(s) 265, 689
SapI GCTCTTC 1 cut(s) 26
SaqAI TTAA 1 cut(s) 657
Sau3AI GATC 3 cut(s) 100, 144, 148
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 137
SfcI CTRYAG 1 cut(s) 807
SfoI GGCGCC 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 265, 689
SmlI CTYRAG 2 cut(s) 354, 971
SmoI CTYRAG 2 cut(s) 354, 971
Sse9I AATT 6 cut(s) 194, 493, 604, 623, 706, 714
SsiI CCGC 2 cut(s) 85, 554
SspDI GGCGCC 1 cut(s) 214
SspI AATATT 1 cut(s) 981
SspMI CTAG 2 cut(s) 366, 749
StyD4I CCNGG 1 cut(s) 135
TaiI ACGT 2 cut(s) 16, 901
TaqI TCGA 4 cut(s) 103, 147, 355, 849
TasI AATT 6 cut(s) 194, 493, 604, 623, 706, 714
TfiI GAWTC 2 cut(s) 362, 869
Tru1I TTAA 1 cut(s) 657
Tru9I TTAA 1 cut(s) 657
TscAI CASTG 2 cut(s) 483, 785
TseFI GTSAC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 7 cut(s) 207, 267, 385, 753, 803, 877, 974
TspRI CASTG 2 cut(s) 483, 785
VspI ATTAAT 1 cut(s) 657
XapI RAATTY 2 cut(s) 493, 714
XhoI CTCGAG 1 cut(s) 354
XspI CTAG 2 cut(s) 366, 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.