Paired amphipathic helix protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 190 |
| AccIII | TCCGGA | 1 cut(s) | 69 |
| AgsI | TTSAA | 2 cut(s) | 196, 309 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 60 |
| AluBI | AGCT | 5 cut(s) | 92, 109, 170, 299, 329 |
| AluI | AGCT | 5 cut(s) | 92, 109, 170, 299, 329 |
| Aor13HI | TCCGGA | 1 cut(s) | 69 |
| AspLEI | GCGC | 1 cut(s) | 192 |
| AsuHPI | GGTGA | 2 cut(s) | 131, 263 |
| BclI | TGATCA | 1 cut(s) | 288 |
| BfaI | CTAG | 1 cut(s) | 167 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 60 |
| BmiI | GGNNCC | 1 cut(s) | 162 |
| BsaJI | CCNNGG | 1 cut(s) | 124 |
| BsaWI | WCCGGW | 1 cut(s) | 69 |
| BseAI | TCCGGA | 1 cut(s) | 69 |
| BseDI | CCNNGG | 1 cut(s) | 124 |
| Bsh1236I | CGCG | 1 cut(s) | 190 |
| BsiSI | CCGG | 1 cut(s) | 70 |
| Bsp13I | TCCGGA | 1 cut(s) | 69 |
| Bsp143I | GATC | 2 cut(s) | 33, 288 |
| BspEI | TCCGGA | 1 cut(s) | 69 |
| BspFNI | CGCG | 1 cut(s) | 190 |
| BspLI | GGNNCC | 1 cut(s) | 162 |
| BssECI | CCNNGG | 1 cut(s) | 124 |
| BssMI | GATC | 2 cut(s) | 33, 288 |
| Bst6I | CTCTTC | 1 cut(s) | 204 |
| BstDEI | CTNAG | 1 cut(s) | 95 |
| BstDSI | CCRYGG | 1 cut(s) | 124 |
| BstEII | GGTNACC | 1 cut(s) | 119 |
| BstFNI | CGCG | 1 cut(s) | 190 |
| BstHHI | GCGC | 1 cut(s) | 192 |
| BstKTI | GATC | 2 cut(s) | 36, 291 |
| BstMBI | GATC | 2 cut(s) | 33, 288 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 167, 196 |
| BstPI | GGTNACC | 1 cut(s) | 119 |
| BstUI | CGCG | 1 cut(s) | 190 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 13 |
| BtgI | CCRYGG | 1 cut(s) | 124 |
| CfoI | GCGC | 1 cut(s) | 192 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 153, 188, 194, 229 |
| CviJI | RGCY | 7 cut(s) | 92, 109, 130, 161, 170, 299, 329 |
| CviKI_1 | RGCY | 7 cut(s) | 92, 109, 130, 161, 170, 299, 329 |
| DdeI | CTNAG | 1 cut(s) | 95 |
| DpnI | GATC | 2 cut(s) | 35, 290 |
| DpnII | GATC | 2 cut(s) | 33, 288 |
| DriI | GACNNNNNGTC | 1 cut(s) | 60 |
| Eam1104I | CTCTTC | 1 cut(s) | 204 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 60 |
| EarI | CTCTTC | 1 cut(s) | 204 |
| Eco91I | GGTNACC | 1 cut(s) | 119 |
| EcoO65I | GGTNACC | 1 cut(s) | 119 |
| FaiI | YATR | 9 cut(s) | 115, 117, 140, 182, 257, 259, 261, 263, 296 |
| FbaI | TGATCA | 1 cut(s) | 288 |
| FspBI | CTAG | 1 cut(s) | 167 |
| GlaI | GCGC | 1 cut(s) | 191 |
| HapII | CCGG | 1 cut(s) | 70 |
| HhaI | GCGC | 1 cut(s) | 192 |
| Hin6I | GCGC | 1 cut(s) | 190 |
| HinP1I | GCGC | 1 cut(s) | 190 |
| HinfI | GANTC | 1 cut(s) | 319 |
| HpaII | CCGG | 1 cut(s) | 70 |
| HphI | GGTGA | 2 cut(s) | 131, 263 |
| Hpy188I | TCNGA | 1 cut(s) | 232 |
| Hpy188III | TCNNGA | 1 cut(s) | 70 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 167, 196 |
| HpyF3I | CTNAG | 1 cut(s) | 95 |
| HspAI | GCGC | 1 cut(s) | 190 |
| Kpn2I | TCCGGA | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 1 cut(s) | 288 |
| Kzo9I | GATC | 2 cut(s) | 33, 288 |
| LmnI | GCTCC | 1 cut(s) | 166 |
| LpnPI | CCDG | 3 cut(s) | 83, 189, 232 |
| MaeI | CTAG | 1 cut(s) | 167 |
| MaeIII | GTNAC | 2 cut(s) | 119, 281 |
| MalI | GATC | 2 cut(s) | 35, 290 |
| MboI | GATC | 2 cut(s) | 33, 288 |
| MboII | GAAGA | 3 cut(s) | 29, 89, 221 |
| MlyI | GAGTC | 1 cut(s) | 313 |
| MnlI | CCTC | 1 cut(s) | 144 |
| MroI | TCCGGA | 1 cut(s) | 69 |
| MseI | TTAA | 4 cut(s) | 51, 185, 237, 331 |
| MspI | CCGG | 1 cut(s) | 70 |
| MvnI | CGCG | 1 cut(s) | 190 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 167, 196 |
| NdeII | GATC | 2 cut(s) | 33, 288 |
| NlaIV | GGNNCC | 1 cut(s) | 162 |
| NmuCI | GTSAC | 2 cut(s) | 119, 281 |
| PleI | GAGTC | 1 cut(s) | 313 |
| PpsI | GAGTC | 1 cut(s) | 313 |
| PspEI | GGTNACC | 1 cut(s) | 119 |
| PspN4I | GGNNCC | 1 cut(s) | 162 |
| SaqAI | TTAA | 4 cut(s) | 51, 185, 237, 331 |
| Sau3AI | GATC | 2 cut(s) | 33, 288 |
| SchI | GAGTC | 1 cut(s) | 313 |
| SetI | ASST | 7 cut(s) | 94, 101, 111, 172, 224, 301, 331 |
| SspMI | CTAG | 1 cut(s) | 167 |
| Tru1I | TTAA | 4 cut(s) | 51, 185, 237, 331 |
| Tru9I | TTAA | 4 cut(s) | 51, 185, 237, 331 |
| TseFI | GTSAC | 2 cut(s) | 119, 281 |
| Tsp45I | GTSAC | 2 cut(s) | 119, 281 |
| XspI | CTAG | 1 cut(s) | 167 |