RLG00000016179

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000544
Physical Location & Seq
Reverse (-)
4493574 .. 4495358
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016179

Sequence Viewer

Length: 561 bp
ATGAAGAACAACGAATCTGTGCCAAATCGCGTTGTGGGCCCTGGCCAGGATGACCCATGCTCAAAGCCGTTTCGATTCAGGGACATCGTATCTGTGGTAGGAAAGGAGCAGCAGCACATATTTCATTTTGATTGCTACTGCTATGGTGTGGAAGTGACGCGCAGGCTTGATGACTATGCCCATGGAAGAAGGGAGACATCAGCCCGTTGCCCTCTCTGCAGAGAAGAAATCAAGTATGGAGAATATCGTACTTATTTACATCTCGAGGAGGGTTTAACACGGCTCCGCCTCGACCAGTATCCACTTCCACCACCACCGCGGCGTCTCCCACCAGTGCCTGCGGGAGATCAGCCTCCACCTCCACCTCCACCTCCTCCTCCCCCGCCGCTGCCTGATGGAGACCAAGATGCTGACATGTTTGAAGATGCCTTTACGGGGCCAGCTCGGGGCGACAACCCAACAACCTATTCTCACACGCATCCTGGTCTCCATCAGGCAGCAGCGGGGGAGGAGGAGGTGGAGGCTGATCTCCGGCAGGTGGGAGACGCCGCGGTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

20.78

Weight (kDa)

5.11

Isoelectric Point (pI)

56.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 526
Acc36I ACCTGC 1 cut(s) 526
AccII CGCG 4 cut(s) 30, 160, 319, 551
AciI CCGC 9 cut(s) 286, 317, 319, 341, 383, 386, 503, 549, 551
AcoI YGGCCR 1 cut(s) 43
AcyI GRCGYC 2 cut(s) 322, 546
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 4 cut(s) 46, 435, 446, 538
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 3 cut(s) 40, 45, 481
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 1 cut(s) 443
AluI AGCT 1 cut(s) 443
Alw26I GTCTC 5 cut(s) 188, 329, 393, 491, 537
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 2 cut(s) 263, 444
AoxI GGCC 3 cut(s) 37, 43, 437
ApaI GGGCCC 1 cut(s) 41
ApeKI GCWGC 5 cut(s) 109, 112, 388, 497, 500
AspLEI GCGC 1 cut(s) 162
AspS9I GGNCC 3 cut(s) 37, 38, 437
AvaI CYCGRG 2 cut(s) 263, 444
BaeGI GKGCMC 1 cut(s) 41
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 41
BbvI GCAGC 5 cut(s) 121, 124, 375, 509, 512
BccI CCATC 2 cut(s) 389, 498
BceAI ACGGC 2 cut(s) 52, 296
BciT130I CCWGG 3 cut(s) 42, 47, 483
BciVI GTATCC 1 cut(s) 309
BcoDI GTCTC 5 cut(s) 188, 329, 393, 491, 537
BfmI CTRYAG 1 cut(s) 217
BfuAI ACCTGC 1 cut(s) 526
BfuI GTATCC 1 cut(s) 309
BisI GCNGC 8 cut(s) 110, 113, 320, 386, 389, 498, 501, 549
BlsI GCNGC 8 cut(s) 111, 114, 321, 387, 390, 499, 502, 550
Bme1390I CCNGG 3 cut(s) 42, 47, 483
BmeT110I CYCGRG 2 cut(s) 263, 444
BmgT120I GGNCC 3 cut(s) 37, 38, 437
BmiI GGNNCC 3 cut(s) 39, 284, 438
BmrFI CCNGG 3 cut(s) 42, 47, 483
BmsI GCATC 3 cut(s) 397, 415, 487
BsaHI GRCGYC 2 cut(s) 322, 546
BsaI GGTCTC 2 cut(s) 393, 491
BsaJI CCNNGG 4 cut(s) 40, 181, 317, 549
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 435, 446, 538
Bse1I ACTGG 2 cut(s) 295, 332
BseBI CCWGG 3 cut(s) 42, 47, 483
BseDI CCNNGG 4 cut(s) 40, 181, 317, 549
BseGI GGATG 2 cut(s) 55, 478
BseLI CCNNNNNNNGG 4 cut(s) 46, 435, 446, 538
BseNI ACTGG 2 cut(s) 295, 332
BseRI GAGGAG 5 cut(s) 281, 363, 366, 524, 527
BseSI GKGCMC 1 cut(s) 41
BseXI GCAGC 5 cut(s) 121, 124, 375, 509, 512
Bsh1236I CGCG 4 cut(s) 30, 160, 319, 551
BshFI GGCC 3 cut(s) 39, 45, 439
BsiHKCI CYCGRG 2 cut(s) 263, 444
BsiSI CCGG 1 cut(s) 532
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 4 cut(s) 46, 435, 446, 538
BsmAI GTCTC 5 cut(s) 188, 329, 393, 491, 537
BsmBI CGTCTC 2 cut(s) 329, 537
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 3 cut(s) 39, 45, 439
Bso31I GGTCTC 2 cut(s) 393, 491
BsoBI CYCGRG 2 cut(s) 263, 444
Bsp120I GGGCCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 2 cut(s) 346, 526
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 9 cut(s) 286, 317, 319, 341, 383, 386, 503, 549, 551
BspANI GGCC 3 cut(s) 39, 45, 439
BspFNI CGCG 4 cut(s) 30, 160, 319, 551
BspLI GGNNCC 3 cut(s) 39, 284, 438
BspMAI CTGCAG 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 526
BspTNI GGTCTC 2 cut(s) 393, 491
BsrI ACTGG 2 cut(s) 295, 332
BssECI CCNNGG 4 cut(s) 40, 181, 317, 549
BssMI GATC 2 cut(s) 346, 526
BssNI GRCGYC 2 cut(s) 322, 546
BssT1I CCWWGG 1 cut(s) 181
Bst2UI CCWGG 3 cut(s) 42, 47, 483
BstACI GRCGYC 2 cut(s) 322, 546
BstC8I GCNNGC 3 cut(s) 164, 339, 441
BstDSI CCRYGG 3 cut(s) 181, 317, 549
BstF5I GGATG 2 cut(s) 55, 478
BstFNI CGCG 4 cut(s) 30, 160, 319, 551
BstHHI GCGC 1 cut(s) 162
BstKTI GATC 2 cut(s) 349, 529
BstMAI GTCTC 5 cut(s) 188, 329, 393, 491, 537
BstMBI GATC 2 cut(s) 346, 526
BstMWI GCNNNNNNNGC 2 cut(s) 36, 216
BstNI CCWGG 3 cut(s) 42, 47, 483
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 40, 45, 481
BstSFI CTRYAG 1 cut(s) 217
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 4 cut(s) 30, 160, 319, 551
BstV1I GCAGC 5 cut(s) 121, 124, 375, 509, 512
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 3 cut(s) 39, 45, 439
BtgI CCRYGG 3 cut(s) 181, 317, 549
BtsCI GGATG 2 cut(s) 55, 478
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 3 cut(s) 164, 339, 441
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 1 cut(s) 162
Cfr13I GGNCC 3 cut(s) 37, 38, 437
Cfr42I CCGCGG 2 cut(s) 320, 552
CseI GACGC 3 cut(s) 166, 311, 554
Csp6I GTAC 1 cut(s) 249
CviAII CATG 3 cut(s) 57, 182, 415
CviQI GTAC 1 cut(s) 249
DpnI GATC 2 cut(s) 348, 528
DpnII GATC 2 cut(s) 346, 526
EaeI YGGCCR 1 cut(s) 43
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 41
Eco31I GGTCTC 2 cut(s) 393, 491
Eco88I CYCGRG 2 cut(s) 263, 444
EcoO109I RGGNCCY 1 cut(s) 38
EcoRII CCWGG 3 cut(s) 40, 45, 481
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 181
Esp3I CGTCTC 2 cut(s) 329, 537
FaeI CATG 3 cut(s) 60, 185, 418
FaiI YATR 7 cut(s) 58, 119, 144, 177, 183, 237, 416
FaqI GGGAC 1 cut(s) 95
FatI CATG 3 cut(s) 56, 181, 414
FauI CCCGC 3 cut(s) 334, 390, 496
Fnu4HI GCNGC 8 cut(s) 110, 113, 320, 386, 389, 498, 501, 549
FokI GGATG 2 cut(s) 62, 465
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 8 cut(s) 110, 113, 320, 386, 389, 498, 501, 549
GlaI GCGC 1 cut(s) 161
GluI GCNGC 8 cut(s) 110, 113, 320, 386, 389, 498, 501, 549
HaeIII GGCC 3 cut(s) 39, 45, 439
HapII CCGG 1 cut(s) 532
HgaI GACGC 3 cut(s) 166, 311, 554
HhaI GCGC 1 cut(s) 162
Hin1I GRCGYC 2 cut(s) 322, 546
Hin1II CATG 3 cut(s) 60, 185, 418
Hin6I GCGC 1 cut(s) 160
HinP1I GCGC 1 cut(s) 160
HinfI GANTC 2 cut(s) 14, 75
HpaII CCGG 1 cut(s) 532
Hpy188III TCNNGA 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 216
Hsp92I GRCGYC 2 cut(s) 322, 546
Hsp92II CATG 3 cut(s) 60, 185, 418
HspAI GCGC 1 cut(s) 160
KspI CCGCGG 2 cut(s) 320, 552
Kzo9I GATC 2 cut(s) 346, 526
LmnI GCTCC 2 cut(s) 106, 288
Lsp1109I GCAGC 5 cut(s) 121, 124, 375, 509, 512
LweI GCATC 3 cut(s) 397, 415, 487
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 348, 528
MboI GATC 2 cut(s) 346, 526
MboII GAAGA 4 cut(s) 16, 198, 236, 434
MhlI GDGCHC 1 cut(s) 41
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 4 cut(s) 319, 388, 503, 551
MspI CCGG 1 cut(s) 532
MspR9I CCNGG 3 cut(s) 42, 47, 483
MvaI CCWGG 3 cut(s) 42, 47, 483
MvnI CGCG 4 cut(s) 30, 160, 319, 551
MwoI GCNNNNNNNGC 2 cut(s) 36, 216
NcoI CCATGG 1 cut(s) 181
NdeII GATC 2 cut(s) 346, 526
NlaIII CATG 3 cut(s) 60, 185, 418
NlaIV GGNNCC 3 cut(s) 39, 284, 438
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 263
PaqCI CACCTGC 1 cut(s) 526
PciI ACATGT 1 cut(s) 414
PfeI GAWTC 2 cut(s) 14, 75
PkrI GCNGC 8 cut(s) 111, 114, 321, 387, 390, 499, 502, 550
PscI ACATGT 1 cut(s) 414
Psp6I CCWGG 3 cut(s) 40, 45, 481
PspGI CCWGG 3 cut(s) 40, 45, 481
PspN4I GGNNCC 3 cut(s) 39, 284, 438
PspOMI GGGCCC 1 cut(s) 37
PspPI GGNCC 3 cut(s) 37, 38, 437
PstI CTGCAG 1 cut(s) 221
PstNI CAGNNNCTG 1 cut(s) 338
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SacII CCGCGG 2 cut(s) 320, 552
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 8 cut(s) 110, 113, 320, 386, 389, 498, 501, 549
Sau3AI GATC 2 cut(s) 346, 526
Sau96I GGNCC 3 cut(s) 37, 38, 437
ScrFI CCNGG 3 cut(s) 42, 47, 483
SduI GDGCHC 1 cut(s) 41
SetI ASST 7 cut(s) 361, 367, 373, 445, 467, 519, 540
SfaNI GCATC 3 cut(s) 397, 415, 487
SfcI CTRYAG 1 cut(s) 217
Sfr274I CTCGAG 1 cut(s) 263
Sfr303I CCGCGG 2 cut(s) 320, 552
SgrBI CCGCGG 2 cut(s) 320, 552
SlaI CTCGAG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 9 cut(s) 286, 317, 319, 341, 383, 386, 503, 549, 551
StyD4I CCNGG 3 cut(s) 40, 45, 481
StyI CCWWGG 1 cut(s) 181
TaqI TCGA 3 cut(s) 73, 264, 291
TauI GCSGC 3 cut(s) 322, 388, 551
TfiI GAWTC 2 cut(s) 14, 75
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 5 cut(s) 109, 112, 388, 497, 500
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 17, 113
TspRI CASTG 1 cut(s) 339
XceI RCATGY 1 cut(s) 418
XhoI CTCGAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.