Rh2DG362200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000544
Physical Location & Seq
Forward (+)
51235392 .. 51237322
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG362200.1

Sequence Viewer

Length: 852 bp
ATGCCGAAGAGAAAACGAGTGCAGGTTTCGACCAGCAGGGTCACCCGAGTCCAGGACGTAATTGACATCGAAGGAACGACGTCGTACTTGGTGAGCAACCGCAGGGGACGTGAACTTTTCAGGCAATCGAAACCTATCTTCCAGAAATACGATAGGGATGTTTTAAATGCGTTGACCAATAGATGGGTCAATGATCAACCATGTTGCTCATTGAATACGGAGAATGTGAAATTGCGAGATAACACGGCCTTCCTGCCTGTGGAAGGTCAAAATGGTTCAAAGCATTACCCTAAAATCTTCTTGCAGTATCTTACTGCTGTTCACGGAGTTGCAGGAAATTTTAGGGCTAGGGATGATCTGTATGCTAAGCTGAAGAAACCGAATCTCACATTGGAACAGTTCAAAAACCTTTATTTCCATTCTTTCCTGCAAAGTGAGGAGGAACTGGCAGATTTCCCTGCACGAATTGTCGACAATATCAAACCTCTAAAGGTTTATTACGAAGGAAAACGATGCAGGTGGGATACAGCGTATTCGTCTTGGGAAGGGAAAAGCTATCAAAGGCAAATTGATCTCATAGGCGCTAGCATGGTGGACTTGCTAGTGTTGAGGGTCTATATGCATGGCAGTTACTCCAACAACGCTGTTAGTGAGGCTCTTCTGGTCCGGAACGTGATCACTCATCTGAATCAGTGGAAACTGAAGAGGGACAGGGACCATTTTGACAAGACTGAGCTCTTAGAGATTGTTTATGAATGCTTCCCGGACATCTGCTTCAAGCTGTTTGATTTCTGTCAGTTTTGTGGAGTCGATATTGACTTTGCAAAACTCCGCAAATTTTTCAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

33.25

Weight (kDa)

9.19

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 507
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 2 cut(s) 13, 507
AccB7I CCANNNNNTGG 1 cut(s) 183
AccI GTMKAC 1 cut(s) 471
AccIII TCCGGA 1 cut(s) 666
AciI CCGC 2 cut(s) 100, 832
AcsI RAATTY 2 cut(s) 337, 836
AcuI CTGAAG 2 cut(s) 392, 722
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 4 cut(s) 52, 183, 259, 263
AgsI TTSAA 5 cut(s) 214, 279, 403, 778, 844
AjiI CACGTC 1 cut(s) 110
AjnI CCWGG 1 cut(s) 51
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 5 cut(s) 370, 555, 736, 781, 848
AluI AGCT 5 cut(s) 370, 555, 736, 781, 848
Alw21I GWGCWC 1 cut(s) 738
Ama87I CYCGRG 1 cut(s) 45
Aor13HI TCCGGA 1 cut(s) 666
AoxI GGCC 1 cut(s) 246
ApoI RAATTY 2 cut(s) 337, 836
AspLEI GCGC 1 cut(s) 584
AspS9I GGNCC 2 cut(s) 664, 715
AsuC2I CCSGG 1 cut(s) 764
AsuHPI GGTGA 2 cut(s) 34, 103
AsuNHI GCTAGC 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 2 cut(s) 664, 715
BanII GRGCYC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 738
BccI CCATC 1 cut(s) 177
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 1 cut(s) 53
BciVI GTATCC 1 cut(s) 517
BclI TGATCA 2 cut(s) 193, 675
BcnI CCSGG 1 cut(s) 764
BfaI CTAG 3 cut(s) 348, 585, 602
BfoI RGCGCY 1 cut(s) 585
BfuAI ACCTGC 2 cut(s) 13, 507
BfuI GTATCC 1 cut(s) 517
BlpI GCTNAGC 1 cut(s) 366
Bme1390I CCNGG 2 cut(s) 53, 764
Bme18I GGWCC 2 cut(s) 664, 715
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 664, 715
BmiI GGNNCC 1 cut(s) 716
BmrFI CCNGG 2 cut(s) 53, 764
BmsI GCATC 1 cut(s) 503
BmtI GCTAGC 1 cut(s) 588
Bpu1102I GCTNAGC 1 cut(s) 366
BpuMI CCSGG 1 cut(s) 764
BsaHI GRCGYC 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 666
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 183, 259, 263
Bse1I ACTGG 1 cut(s) 450
BseAI TCCGGA 1 cut(s) 666
BseBI CCWGG 1 cut(s) 53
BseGI GGATG 2 cut(s) 163, 358
BseLI CCNNNNNNNGG 4 cut(s) 52, 183, 259, 263
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 452
BsgI GTGCAG 2 cut(s) 41, 444
BshFI GGCC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 738
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 2 cut(s) 667, 764
BslFI GGGAC 3 cut(s) 120, 722, 728
BslI CCNNNNNNNGG 4 cut(s) 52, 183, 259, 263
BsmFI GGGAC 3 cut(s) 120, 722, 728
BsmI GAATGC 1 cut(s) 761
BsnI GGCC 1 cut(s) 248
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 738
Bsp13I TCCGGA 1 cut(s) 666
Bsp143I GATC 4 cut(s) 193, 355, 571, 675
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 2 cut(s) 100, 832
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 724
BspEI TCCGGA 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 716
BspMI ACCTGC 2 cut(s) 13, 507
BspOI GCTAGC 1 cut(s) 588
BspQI GCTCTTC 1 cut(s) 663
BsrI ACTGG 1 cut(s) 450
BssMI GATC 4 cut(s) 193, 355, 571, 675
BssNI GRCGYC 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 399
Bst6I CTCTTC 3 cut(s) 2, 663, 698
BstACI GRCGYC 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 3 cut(s) 366, 732, 739
BstEII GGTNACC 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 163, 358
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 1 cut(s) 584
BstKTI GATC 4 cut(s) 196, 358, 574, 678
BstMBI GATC 4 cut(s) 193, 355, 571, 675
BstNI CCWGG 1 cut(s) 53
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 2 cut(s) 51, 762
BsuI GTATCC 1 cut(s) 517
BsuRI GGCC 1 cut(s) 248
BtrI CACGTC 1 cut(s) 110
BtsCI GGATG 2 cut(s) 163, 358
BtsIMutI CAGTG 1 cut(s) 698
BveI ACCTGC 2 cut(s) 13, 507
Cac8I GCNNGC 1 cut(s) 586
CfoI GCGC 1 cut(s) 584
Cfr13I GGNCC 2 cut(s) 664, 715
Csp6I GTAC 1 cut(s) 85
CviAII CATG 3 cut(s) 201, 589, 623
CviJI RGCY 8 cut(s) 248, 347, 370, 555, 656, 736, 781, 848
CviKI_1 RGCY 8 cut(s) 248, 347, 370, 555, 656, 736, 781, 848
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 3 cut(s) 366, 732, 739
DpnI GATC 4 cut(s) 195, 357, 573, 677
DpnII GATC 4 cut(s) 193, 355, 571, 675
DraI TTTAAA 1 cut(s) 165
Eam1104I CTCTTC 3 cut(s) 2, 663, 698
EarI CTCTTC 3 cut(s) 2, 663, 698
Ecl136II GAGCTC 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 738
Eco47I GGWCC 2 cut(s) 664, 715
Eco53kI GAGCTC 1 cut(s) 736
Eco57I CTGAAG 2 cut(s) 392, 722
Eco88I CYCGRG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 736
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO65I GGTNACC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 738
FaeI CATG 3 cut(s) 204, 592, 626
FaiI YATR 8 cut(s) 202, 363, 578, 590, 618, 620, 624, 753
FaqI GGGAC 3 cut(s) 120, 722, 728
FatI CATG 3 cut(s) 200, 588, 622
FbaI TGATCA 2 cut(s) 193, 675
FblI GTMKAC 1 cut(s) 471
FokI GGATG 2 cut(s) 170, 365
FriOI GRGCYC 1 cut(s) 738
FspBI CTAG 3 cut(s) 348, 585, 602
GlaI GCGC 1 cut(s) 583
HaeII RGCGCY 1 cut(s) 585
HaeIII GGCC 1 cut(s) 248
HapII CCGG 2 cut(s) 667, 764
HhaI GCGC 1 cut(s) 584
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 3 cut(s) 204, 592, 626
Hin6I GCGC 1 cut(s) 582
HinP1I GCGC 1 cut(s) 582
HincII GTYRAC 2 cut(s) 174, 472
HindII GTYRAC 2 cut(s) 174, 472
HindIII AAGCTT 1 cut(s) 846
HinfI GANTC 4 cut(s) 48, 382, 688, 807
HpaII CCGG 2 cut(s) 667, 764
HphI GGTGA 2 cut(s) 34, 103
Hpy166II GTNNAC 5 cut(s) 113, 174, 322, 472, 595
Hpy188I TCNGA 1 cut(s) 687
Hpy188III TCNNGA 2 cut(s) 142, 667
Hpy8I GTNNAC 5 cut(s) 113, 174, 322, 472, 595
Hpy99I CGWCG 2 cut(s) 82, 85
HpyAV CCTTC 5 cut(s) 65, 257, 259, 497, 539
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4IV ACGT 4 cut(s) 57, 80, 109, 672
HpyCH4V TGCA 8 cut(s) 22, 304, 332, 430, 461, 516, 622, 824
HpyF3I CTNAG 3 cut(s) 366, 732, 739
HpySE526I ACGT 4 cut(s) 57, 80, 109, 672
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 3 cut(s) 204, 592, 626
HspAI GCGC 1 cut(s) 582
Kpn2I TCCGGA 1 cut(s) 666
Ksp22I TGATCA 2 cut(s) 193, 675
Kzo9I GATC 4 cut(s) 193, 355, 571, 675
LguI GCTCTTC 1 cut(s) 663
LweI GCATC 1 cut(s) 503
MaeI CTAG 3 cut(s) 348, 585, 602
MaeII ACGT 4 cut(s) 57, 80, 109, 672
MaeIII GTNAC 2 cut(s) 40, 629
MalI GATC 4 cut(s) 195, 357, 573, 677
MboI GATC 4 cut(s) 193, 355, 571, 675
MboII GAAGA 6 cut(s) 19, 130, 289, 385, 650, 715
MhlI GDGCHC 1 cut(s) 738
MluCI AATT 6 cut(s) 60, 230, 337, 465, 567, 836
MlyI GAGTC 2 cut(s) 57, 816
MmeI TCCRAC 1 cut(s) 660
MnlI CCTC 6 cut(s) 430, 433, 495, 603, 646, 699
Mph1103I ATGCAT 1 cut(s) 624
MroI TCCGGA 1 cut(s) 666
MseI TTAA 1 cut(s) 164
MspI CCGG 2 cut(s) 667, 764
MspR9I CCNGG 2 cut(s) 53, 764
Mva1269I GAATGC 1 cut(s) 761
MvaI CCWGG 1 cut(s) 53
NciI CCSGG 1 cut(s) 764
NdeII GATC 4 cut(s) 193, 355, 571, 675
NheI GCTAGC 1 cut(s) 584
NlaIII CATG 3 cut(s) 204, 592, 626
NlaIV GGNNCC 1 cut(s) 716
NmuCI GTSAC 1 cut(s) 40
NsiI ATGCAT 1 cut(s) 624
PaqCI CACCTGC 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 761
PfeI GAWTC 2 cut(s) 382, 688
PflMI CCANNNNNTGG 1 cut(s) 183
PfoI TCCNGGA 2 cut(s) 51, 762
PleI GAGTC 2 cut(s) 56, 815
PpsI GAGTC 2 cut(s) 56, 815
Psp124BI GAGCTC 1 cut(s) 738
Psp6I CCWGG 1 cut(s) 51
PspEI GGTNACC 1 cut(s) 40
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 716
PspPI GGNCC 2 cut(s) 664, 715
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacI GAGCTC 1 cut(s) 738
SalI GTCGAC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 1 cut(s) 164
Sau3AI GATC 4 cut(s) 193, 355, 571, 675
Sau96I GGNCC 2 cut(s) 664, 715
SchI GAGTC 2 cut(s) 57, 816
ScrFI CCNGG 2 cut(s) 53, 764
SduI GDGCHC 1 cut(s) 738
SfaNI GCATC 1 cut(s) 503
SinI GGWCC 2 cut(s) 664, 715
Sse9I AATT 6 cut(s) 60, 230, 337, 465, 567, 836
SsiI CCGC 2 cut(s) 100, 832
SspMI CTAG 3 cut(s) 348, 585, 602
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 2 cut(s) 51, 762
TaaI ACNGT 1 cut(s) 399
TaiI ACGT 4 cut(s) 60, 83, 112, 675
TaqI TCGA 5 cut(s) 29, 69, 128, 471, 810
TasI AATT 6 cut(s) 60, 230, 337, 465, 567, 836
TfiI GAWTC 2 cut(s) 382, 688
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 1 cut(s) 698
TseFI GTSAC 1 cut(s) 40
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 768
TspGWI ACGGA 2 cut(s) 233, 339
TspRI CASTG 1 cut(s) 698
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 2 cut(s) 664, 715
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 2 cut(s) 337, 836
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 3 cut(s) 348, 585, 602
ZraI GACGTC 1 cut(s) 81
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.