Rh2AG050300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000544
Physical Location & Seq
Forward (+)
3976013 .. 3976857
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG050300.1

Sequence Viewer

Length: 579 bp
ATGGCCAAATGGGGGAGATCATCTTCAACCGAGCGAAGACCTAAAAACCGATCTGTGGCGGTTGAAGATGATCTCCCCCGTTTGGCCATGACAGATCGGTTGGAAGTCAGGGATCACGTCGCAATTCTCCCCGCAGTTGGCTCTGGTCTTGATGAGGATAAGGATTATCAGAATAAATTCAGGGAGGCCGTGGAAGCGTTTCTCGGATTTAGTGGAACACATCACCACGATAGAGACAACTTCTTCCGCTGGCTTCGAACCCCCTTATCAAGGGAAAGATTCGCTGCTCTTTATTTTCATCGTTTACTGCTCAATGAAGCACAGAGGGCCGATTTTCCGGCTGATACCGTAGAGAACCTCAGGAATAGTGTTGTTCGTTATAGGGGCGTGAATTATAGTTTTGAGGATGGCTACAACATCTGGGAGATGATCGAGTGGAGGGGACAGTACAATTTAGCAGTTACCAGTCAGAAGAACAGTGGTTTAGAGATGCATACAACTTCCGAACCTACAAGGACACTGCCCTTGATGAGGCGCAGCTGGTCCGAAATACTACTCGACATTTCAATGACAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

22.47

Weight (kDa)

6.6

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 59, 132, 247
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 2 cut(s) 3, 84
AcsI RAATTY 1 cut(s) 176
AfaI GTAC 1 cut(s) 449
AfiI CCNNNNNNNGG 6 cut(s) 12, 55, 82, 137, 270, 531
AgsI TTSAA 3 cut(s) 27, 65, 567
AjiI CACGTC 1 cut(s) 118
AluBI AGCT 1 cut(s) 540
AluI AGCT 1 cut(s) 540
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 1 cut(s) 120
AoxI GGCC 4 cut(s) 3, 84, 186, 327
ApeKI GCWGC 2 cut(s) 284, 537
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 2 cut(s) 176, 198
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 2 cut(s) 327, 543
AsuHPI GGTGA 1 cut(s) 215
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 543
AxyI CCTNAGG 1 cut(s) 359
BalI TGGCCA 2 cut(s) 5, 86
BbsI GAAGAC 1 cut(s) 43
BbvI GCAGC 2 cut(s) 271, 549
BccI CCATC 1 cut(s) 401
BceAI ACGGC 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 228
BisI GCNGC 2 cut(s) 285, 538
BlsI GCNGC 2 cut(s) 286, 539
Bme18I GGWCC 1 cut(s) 543
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 327, 543
BmsI GCATC 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 43
Bpu14I TTCGAA 1 cut(s) 256
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 55, 82, 137, 270, 531
Bse1I ACTGG 1 cut(s) 465
Bse21I CCTNAGG 1 cut(s) 359
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 6 cut(s) 12, 55, 82, 137, 270, 531
BseMII CTCAG 1 cut(s) 373
BseNI ACTGG 1 cut(s) 465
BseXI GCAGC 2 cut(s) 271, 549
BshFI GGCC 4 cut(s) 5, 86, 188, 329
BsiSI CCGG 1 cut(s) 338
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 6 cut(s) 12, 55, 82, 137, 270, 531
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 4 cut(s) 5, 86, 188, 329
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 6 cut(s) 17, 50, 70, 94, 112, 429
BspACI CCGC 3 cut(s) 59, 132, 247
BspANI GGCC 4 cut(s) 5, 86, 188, 329
BspCNI CTCAG 1 cut(s) 372
BspPI GGATC 1 cut(s) 120
BspT104I TTCGAA 1 cut(s) 256
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 6 cut(s) 17, 50, 70, 94, 112, 429
Bst4CI ACNGT 3 cut(s) 349, 447, 479
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 1 cut(s) 359
BstDSI CCRYGG 1 cut(s) 189
BstENI CCTNNNNNAGG 2 cut(s) 268, 529
BstF5I GGATG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 537
BstKTI GATC 6 cut(s) 20, 53, 73, 97, 115, 432
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 6 cut(s) 17, 50, 70, 94, 112, 429
BstMWI GCNNNNNNNGC 2 cut(s) 194, 326
BstV1I GCAGC 2 cut(s) 271, 549
BstV2I GAAGAC 1 cut(s) 43
Bsu36I CCTNAGG 1 cut(s) 359
BsuRI GGCC 4 cut(s) 5, 86, 188, 329
BtgI CCRYGG 1 cut(s) 189
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 2 cut(s) 484, 518
Cac8I GCNNGC 1 cut(s) 251
CfoI GCGC 1 cut(s) 537
Cfr13I GGNCC 2 cut(s) 327, 543
Csp6I GTAC 1 cut(s) 448
CviAII CATG 2 cut(s) 88, 576
CviJI RGCY 9 cut(s) 5, 86, 141, 188, 253, 329, 341, 411, 540
CviKI_1 RGCY 9 cut(s) 5, 86, 141, 188, 253, 329, 341, 411, 540
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 1 cut(s) 359
DpnI GATC 6 cut(s) 19, 52, 72, 96, 114, 431
DpnII GATC 6 cut(s) 17, 50, 70, 94, 112, 429
EaeI YGGCCR 2 cut(s) 3, 84
Eco47I GGWCC 1 cut(s) 543
Eco81I CCTNAGG 1 cut(s) 359
EcoNI CCTNNNNNAGG 2 cut(s) 268, 529
EcoT22I ATGCAT 1 cut(s) 495
FaeI CATG 2 cut(s) 91, 579
FaiI YATR 5 cut(s) 89, 381, 396, 495, 577
FaqI GGGAC 1 cut(s) 456
FatI CATG 2 cut(s) 87, 575
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 2 cut(s) 285, 538
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 2 cut(s) 285, 538
GlaI GCGC 1 cut(s) 536
GluI GCNGC 2 cut(s) 285, 538
HaeIII GGCC 4 cut(s) 5, 86, 188, 329
HapII CCGG 1 cut(s) 338
HhaI GCGC 1 cut(s) 537
Hin1II CATG 2 cut(s) 91, 579
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 1 cut(s) 279
HpaII CCGG 1 cut(s) 338
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 5 cut(s) 171, 206, 471, 505, 547
Hpy188III TCNNGA 2 cut(s) 149, 361
Hpy8I GTNNAC 1 cut(s) 305
Hpy99I CGWCG 1 cut(s) 122
HpyCH4III ACNGT 3 cut(s) 349, 447, 479
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 326
HpyF3I CTNAG 1 cut(s) 359
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 2 cut(s) 91, 579
HspAI GCGC 1 cut(s) 535
Kzo9I GATC 6 cut(s) 17, 50, 70, 94, 112, 429
LpnPI CCDG 9 cut(s) 94, 129, 166, 235, 346, 351, 406, 478, 526
Lsp1109I GCAGC 2 cut(s) 271, 549
LweI GCATC 1 cut(s) 480
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 460
MalI GATC 6 cut(s) 19, 52, 72, 96, 114, 431
MboI GATC 6 cut(s) 17, 50, 70, 94, 112, 429
MboII GAAGA 5 cut(s) 15, 48, 77, 235, 484
MlsI TGGCCA 2 cut(s) 5, 86
MluCI AATT 4 cut(s) 123, 176, 391, 451
MluNI TGGCCA 2 cut(s) 5, 86
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 7 cut(s) 148, 178, 318, 368, 397, 432, 525
Mox20I TGGCCA 2 cut(s) 5, 86
Mph1103I ATGCAT 1 cut(s) 495
MroXI GAANNNNTTC 2 cut(s) 176, 198
MscI TGGCCA 2 cut(s) 5, 86
MslI CAYNNNNRTG 1 cut(s) 566
Msp20I TGGCCA 2 cut(s) 5, 86
MspA1I CMGCKG 2 cut(s) 249, 540
MspI CCGG 1 cut(s) 338
MwoI GCNNNNNNNGC 2 cut(s) 194, 326
NdeII GATC 6 cut(s) 17, 50, 70, 94, 112, 429
NlaIII CATG 2 cut(s) 91, 579
NsiI ATGCAT 1 cut(s) 495
NspV TTCGAA 1 cut(s) 256
PdmI GAANNNNTTC 2 cut(s) 176, 198
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 2 cut(s) 286, 539
PspPI GGNCC 2 cut(s) 327, 543
PvuII CAGCTG 1 cut(s) 540
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 1 cut(s) 566
SatI GCNGC 2 cut(s) 285, 538
Sau3AI GATC 6 cut(s) 17, 50, 70, 94, 112, 429
Sau96I GGNCC 2 cut(s) 327, 543
SetI ASST 5 cut(s) 43, 120, 360, 511, 542
SfaNI GCATC 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 566
Sse9I AATT 4 cut(s) 123, 176, 391, 451
SsiI CCGC 3 cut(s) 59, 132, 247
TaaI ACNGT 3 cut(s) 349, 447, 479
TaiI ACGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 256, 432, 558
TasI AATT 4 cut(s) 123, 176, 391, 451
TatI WGTACW 1 cut(s) 447
TfiI GAWTC 1 cut(s) 279
TscAI CASTG 2 cut(s) 484, 525
TseI GCWGC 2 cut(s) 284, 537
TspDTI ATGAA 2 cut(s) 287, 330
TspRI CASTG 2 cut(s) 484, 525
VpaK11BI GGWCC 1 cut(s) 543
XagI CCTNNNNNAGG 2 cut(s) 268, 529
XapI RAATTY 1 cut(s) 176
XmnI GAANNNNTTC 2 cut(s) 176, 198
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.