FvH4_5g13221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000512
Physical Location & Seq
Reverse (-)
7466956 .. 7467626
671 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13221.t1

Sequence Viewer

Length: 504 bp
ATGAAGTTTACTATGGAGAATGCCAAGGCATCTGTGCTCGGAGTCATTGCAAAACTTAAGGGTTGGGTTCAGAAGGATTCTCCTGACGAAGAAATGGCACAACCACCACCACCGCCACCAGCAGAGCCCAAACAAGGGAACCACAACCTGCTAGATTACTGGGGCAAGAAACTTGTTGTGCTCTGCTCGGGGTCGGCCATCGATATGGCACTCCTCTCCGCCCAGATCCACTCTCAGCTTCCCGTAATCTTCTGCTTCCTCGGATTAACCATCATTCTTGCCTTTACTTGTTTCTTTGTAGGAAAATGTGTCCGCTCCAACTTTGAGCAAGCGGCTCAGGTGTTCGAGCGGATCGGCATTTTCTTTGGTGTCACGGCAATTTTCATAGCCATCTGTGTTCCATTTCCTCTGTGGTTTAAGTGTGTCGCCTGCTTCATCTATGCGCTCTGCTGGTTTACCATCTTGTATACTCGTGGAGGGTTTGTGGCTATTGCTCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.46

Weight (kDa)

8.43

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 156
AccBSI CCGCTC 2 cut(s) 315, 349
AccI GTMKAC 1 cut(s) 467
AciI CCGC 5 cut(s) 113, 219, 313, 332, 349
AclWI GGATC 2 cut(s) 220, 359
AcoI YGGCCR 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 134, 135
AflII CTTAAG 1 cut(s) 56
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw21I GWGCWC 2 cut(s) 39, 183
AlwI GGATC 2 cut(s) 220, 359
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 187
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 1 cut(s) 471
Bbv12I GWGCWC 2 cut(s) 39, 183
BccI CCATC 4 cut(s) 206, 278, 398, 467
BceAI ACGGC 1 cut(s) 390
BfaI CTAG 1 cut(s) 152
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 156
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 336
Bsa29I ATCGAT 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 24, 259
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 135
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 45
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 24, 259
BseLI CCNNNNNNNGG 2 cut(s) 134, 135
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 248, 350
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 1 cut(s) 203
BshFI GGCC 1 cut(s) 197
BshVI ATCGAT 1 cut(s) 201
BsiHKAI GWGCWC 2 cut(s) 39, 183
BsiHKCI CYCGRG 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 134, 135
BsmI GAATGC 1 cut(s) 25
BsnI GGCC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 3 cut(s) 39, 129, 183
Bsp143I GATC 2 cut(s) 225, 351
BspACI CCGC 5 cut(s) 113, 219, 313, 332, 349
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 247, 349
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 140
BspMI ACCTGC 1 cut(s) 156
BspPI GGATC 2 cut(s) 220, 359
BspTI CTTAAG 1 cut(s) 56
BsrBI CCGCTC 2 cut(s) 315, 349
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 24, 259
BssMI GATC 2 cut(s) 225, 351
BssNAI GTATAC 1 cut(s) 468
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 24
Bst1107I GTATAC 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 471
BstAFI CTTAAG 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 330, 430
BstDEI CTNAG 3 cut(s) 234, 336, 501
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 2 cut(s) 228, 354
BstMBI GATC 2 cut(s) 225, 351
BstX2I RGATCY 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 225
BstZ17I GTATAC 1 cut(s) 468
Bsu15I ATCGAT 1 cut(s) 201
BsuRI GGCC 1 cut(s) 197
BsuTUI ATCGAT 1 cut(s) 201
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 330, 430
CfoI GCGC 1 cut(s) 445
ClaI ATCGAT 1 cut(s) 201
CviJI RGCY 6 cut(s) 127, 197, 238, 335, 389, 488
CviKI_1 RGCY 6 cut(s) 127, 197, 238, 335, 389, 488
DdeI CTNAG 3 cut(s) 234, 336, 501
DpnI GATC 2 cut(s) 227, 353
DpnII GATC 2 cut(s) 225, 351
EaeI YGGCCR 1 cut(s) 195
EciI GGCGGA 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 24
Eco24I GRGCYC 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 24
FaiI YATR 5 cut(s) 14, 206, 386, 441, 468
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 333
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 1 cut(s) 152
GlaI GCGC 1 cut(s) 444
GluI GCNGC 1 cut(s) 333
HaeIII GGCC 1 cut(s) 197
HhaI GCGC 1 cut(s) 445
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HinfI GANTC 2 cut(s) 42, 77
Hpy166II GTNNAC 3 cut(s) 9, 456, 468
Hpy188I TCNGA 3 cut(s) 41, 72, 263
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 3 cut(s) 9, 456, 468
HpyAV CCTTC 1 cut(s) 67
HpyCH4V TGCA 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 234, 336, 501
HspAI GCGC 1 cut(s) 443
Kzo9I GATC 2 cut(s) 225, 351
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 8 cut(s) 96, 132, 145, 161, 236, 323, 436, 442
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 152
MaeIII GTNAC 1 cut(s) 370
MalI GATC 2 cut(s) 227, 353
MbiI CCGCTC 2 cut(s) 315, 349
MboI GATC 2 cut(s) 225, 351
MboII GAAGA 2 cut(s) 101, 241
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 3 cut(s) 39, 129, 183
MluCI AATT 1 cut(s) 378
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 4 cut(s) 224, 269, 417, 470
MseI TTAA 3 cut(s) 57, 266, 417
MslI CAYNNNNRTG 1 cut(s) 203
MspCI CTTAAG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 25
NdeII GATC 2 cut(s) 225, 351
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 370
PctI GAATGC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 1 cut(s) 334
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 140
PsuI RGATCY 1 cut(s) 225
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 3 cut(s) 57, 266, 417
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 2 cut(s) 225, 351
SchI GAGTC 1 cut(s) 51
SduI GDGCHC 3 cut(s) 39, 129, 183
SetI ASST 3 cut(s) 150, 240, 342
SfaNI GCATC 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 1 cut(s) 378
SsiI CCGC 5 cut(s) 113, 219, 313, 332, 349
SspMI CTAG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 24
TaqI TCGA 2 cut(s) 201, 345
TasI AATT 1 cut(s) 378
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 57, 266, 417
Tru9I TTAA 3 cut(s) 57, 266, 417
TseFI GTSAC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 3 cut(s) 17, 373, 424
Vha464I CTTAAG 1 cut(s) 56
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.