MD03G1010100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000512
Physical Location & Seq
Reverse (-)
769261 .. 770863
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010100.v1.1.491

Sequence Viewer

Length: 474 bp
ATGAAGAACAATATTGCTAGTGCTTATCGGTGCCTTCGGGCATTTTTTCAGCAACAACTCAACAAGGTCATCAACGCGTTGGATAATAATCTTGACCCAGAAGCAATAGTACCGGCTGATCAACCACCGCTGCGGCCAGCCACGGCCACCAGCTCTAGGACTTCGAAGCACAACTTAGACTGGGCAAAGATTATCATGGCGTATTGCTTGTCGACAGCTATTGGAATGGCTCTCGTTCCCATCCAACTTCATTCCGACCATCTCCCTCTAACTTTTTGCGTTCTCGGTCTCGCAATCCTACTAGCCTTTACTTGTATAATGGTTAGCAAATTTATCCAGCACTCCAACTCTCCACGGAGAATAGTTCATCTCTTCCATTATTTCGGGGTCTTCTTTGGAGTCACCGCTTTCTTCATTTCCGTCACAATCCCATTTCCTGTCTGGTTCAAATGGAGCAGCAGACATATGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.74

Weight (kDa)

9.64

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 212
AccII CGCG 1 cut(s) 77
AciI CCGC 3 cut(s) 128, 133, 405
AcoI YGGCCR 2 cut(s) 134, 144
AcsI RAATTY 1 cut(s) 329
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 1 cut(s) 448
AluBI AGCT 3 cut(s) 153, 218, 471
AluI AGCT 3 cut(s) 153, 218, 471
Alw26I GTCTC 1 cut(s) 293
AoxI GGCC 2 cut(s) 134, 144
ApeKI GCWGC 2 cut(s) 130, 456
ApoI RAATTY 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 394
AsuII TTCGAA 1 cut(s) 164
BanI GGYRCC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 93, 125
BbsI GAAGAC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 117, 468
BccI CCATC 2 cut(s) 248, 267
BceAI ACGGC 1 cut(s) 159
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 3 cut(s) 18, 156, 302
BisI GCNGC 3 cut(s) 131, 134, 457
BlsI GCNGC 3 cut(s) 132, 135, 458
BmiI GGNNCC 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 382
Bpu14I TTCGAA 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 141, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 141, 353
BseGI GGATG 1 cut(s) 240
BseJI GATNNNNATC 1 cut(s) 87
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 117, 468
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 136, 146
BshNI GGYRCC 1 cut(s) 30
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 293
BsnI GGCC 2 cut(s) 136, 146
Bso31I GGTCTC 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 164
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 3 cut(s) 128, 133, 405
BspANI GGCC 2 cut(s) 136, 146
BspFNI CGCG 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 32
BspT104I TTCGAA 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 141, 353
BssMI GATC 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 175
BstDSI CCRYGG 2 cut(s) 141, 353
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 1 cut(s) 118
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 117, 468
BstV2I GAAGAC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 136, 146
BtgI CCRYGG 2 cut(s) 141, 353
BtsCI GGATG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 112
Csp6I GTAC 1 cut(s) 110
CviAII CATG 1 cut(s) 196
CviJI RGCY 9 cut(s) 116, 136, 140, 146, 153, 218, 230, 305, 471
CviKI_1 RGCY 9 cut(s) 116, 136, 140, 146, 153, 218, 230, 305, 471
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 1 cut(s) 175
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 134, 144
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 293
FaeI CATG 1 cut(s) 199
FaiI YATR 4 cut(s) 197, 317, 465, 467
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FatI CATG 1 cut(s) 195
FauNDI CATATG 1 cut(s) 465
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 131, 134, 457
FokI GGATG 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 131, 134, 457
FspBI CTAG 3 cut(s) 18, 156, 302
GluI GCNGC 3 cut(s) 131, 134, 457
HaeIII GGCC 2 cut(s) 136, 146
HapII CCGG 1 cut(s) 113
Hin1II CATG 1 cut(s) 199
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 1 cut(s) 399
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 1 cut(s) 213
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 1 cut(s) 213
HpyAV CCTTC 1 cut(s) 44
HpyF3I CTNAG 1 cut(s) 175
Hsp92II CATG 1 cut(s) 199
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 1 cut(s) 118
LmnI GCTCC 1 cut(s) 453
LpnPI CCDG 8 cut(s) 111, 126, 150, 163, 166, 350, 427, 450
Lsp1109I GCAGC 2 cut(s) 117, 468
MaeI CTAG 3 cut(s) 18, 156, 302
MaeIII GTNAC 2 cut(s) 400, 421
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 4 cut(s) 16, 364, 382, 403
MluCI AATT 1 cut(s) 329
MluI ACGCGT 1 cut(s) 75
MlyI GAGTC 1 cut(s) 408
MmeI TCCRAC 4 cut(s) 60, 268, 279, 369
MnlI CCTC 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 77
NdeI CATATG 1 cut(s) 465
NdeII GATC 1 cut(s) 118
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 400, 421
NspV TTCGAA 1 cut(s) 164
PkrI GCNGC 3 cut(s) 132, 135, 458
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PspN4I GGNNCC 1 cut(s) 32
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SalI GTCGAC 1 cut(s) 211
SatI GCNGC 3 cut(s) 131, 134, 457
Sau3AI GATC 1 cut(s) 118
SchI GAGTC 1 cut(s) 408
SetI ASST 4 cut(s) 69, 155, 220, 473
SfuI TTCGAA 1 cut(s) 164
Sse9I AATT 1 cut(s) 329
SsiI CCGC 3 cut(s) 128, 133, 405
SspI AATATT 1 cut(s) 13
SspMI CTAG 3 cut(s) 18, 156, 302
TaqI TCGA 2 cut(s) 164, 212
TaqII GACCGA 1 cut(s) 275
TasI AATT 1 cut(s) 329
TauI GCSGC 1 cut(s) 136
TseFI GTSAC 2 cut(s) 400, 421
TseI GCWGC 2 cut(s) 130, 456
Tsp45I GTSAC 2 cut(s) 400, 421
TspDTI ATGAA 4 cut(s) 17, 239, 356, 403
TspGWI ACGGA 2 cut(s) 370, 409
XapI RAATTY 1 cut(s) 329
XcmI CCANNNNNNNNNTGG 1 cut(s) 438
XmiI GTMKAC 1 cut(s) 212
XspI CTAG 3 cut(s) 18, 156, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.