Rmu_sc0004712.1_g000028

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004712.1
OG0000512
Physical Location & Seq
Reverse (-)
115117 .. 115731
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004712.1_g000028.1.cds

Sequence Viewer

Length: 615 bp
atgcaacgtccgctcgtgggtccatcgatccgtcgccccgtcggccacttttctttggtagttcaatcactctatacatgtggcaaacatcaacactccttctcttcacattcacatgcttgctctcctttctttgttcctggctttccattttttcgccaccaaatagctgctagtaaaatgagttttgctgcagctattacaaaactgctgcagctagttcagaaaatgctagcagtggcgcctgatgatcaagttccacggcgccaaaacttagactgggctaagataattgtggtgtttagcttggcatcagcaatcgacataactctcctatcagtccaagttcgcttccaccttcctatagtcttctactttctcgagcttgctatcttgcttgcctttacttgtttatttacaagcaagcaagtctattccaactgtccactagtctctcaggcactcgagcagtttggcatcttctttggagtcagtgccttcttcatatgcatcacagttccttttccggctgtcaggttcaaatgcattgcatgcttcatctacgcagtctcatggcttgtgatattcttttgtaattattgcttctgtgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.99

Weight (kDa)

9.23

Isoelectric Point (pI)

48.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 241, 264
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 1 cut(s) 11
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 43
AcyI GRCGYC 2 cut(s) 242, 265
AfiI CCNNNNNNNGG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 77
AgsI TTSAA 2 cut(s) 65, 541
AhlI ACTAGT 1 cut(s) 448
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 5 cut(s) 170, 197, 217, 306, 385
AluI AGCT 5 cut(s) 170, 197, 217, 306, 385
Alw26I GTCTC 2 cut(s) 457, 574
AlwI GGATC 1 cut(s) 22
Ama87I CYCGRG 2 cut(s) 380, 464
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 5 cut(s) 170, 191, 194, 211, 214
AspLEI GCGC 2 cut(s) 244, 267
AspS9I GGNCC 1 cut(s) 20
AsuNHI GCTAGC 1 cut(s) 232
AvaI CYCGRG 2 cut(s) 380, 464
AvaII GGWCC 1 cut(s) 20
BanI GGYRCC 2 cut(s) 241, 264
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 361
BbvI GCAGC 5 cut(s) 157, 178, 198, 206, 226
BccI CCATC 1 cut(s) 31
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 141
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 2 cut(s) 457, 574
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 4 cut(s) 174, 218, 233, 449
BfmI CTRYAG 3 cut(s) 192, 212, 363
BfoI RGCGCY 2 cut(s) 245, 268
BglI GCCNNNNNGGC 1 cut(s) 42
BisI GCNGC 5 cut(s) 171, 192, 195, 212, 215
BlsI GCNGC 5 cut(s) 172, 193, 196, 213, 216
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 1 cut(s) 20
BmeT110I CYCGRG 2 cut(s) 380, 464
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 3 cut(s) 21, 243, 266
BmrFI CCNGG 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 3 cut(s) 320, 486, 519
BmtI GCTAGC 1 cut(s) 236
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 361
Bsa29I ATCGAT 1 cut(s) 26
BsaHI GRCGYC 2 cut(s) 242, 265
BsaJI CCNNGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 1 cut(s) 141
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 284
BseXI GCAGC 5 cut(s) 157, 178, 198, 206, 226
BshFI GGCC 1 cut(s) 45
BshNI GGYRCC 2 cut(s) 241, 264
BshVI ATCGAT 1 cut(s) 26
BsiHKCI CYCGRG 2 cut(s) 380, 464
BsiSI CCGG 1 cut(s) 527
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 2 cut(s) 457, 574
BsnI GGCC 1 cut(s) 45
BsoBI CYCGRG 2 cut(s) 380, 464
Bsp143I GATC 2 cut(s) 27, 250
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 469
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 3 cut(s) 21, 243, 266
BspMAI CTGCAG 2 cut(s) 196, 216
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 2 cut(s) 241, 264
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 2 cut(s) 27, 250
BssNI GRCGYC 2 cut(s) 242, 265
BssSI CACGAG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 443, 517
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 2 cut(s) 242, 265
BstAPI GCANNNNNTGC 1 cut(s) 552
BstC8I GCNNGC 6 cut(s) 121, 234, 387, 399, 425, 553
BstDEI CTNAG 3 cut(s) 274, 285, 456
BstDSI CCRYGG 1 cut(s) 260
BstH2I RGCGCY 2 cut(s) 245, 268
BstHHI GCGC 2 cut(s) 244, 267
BstKTI GATC 2 cut(s) 30, 253
BstMAI GTCTC 2 cut(s) 457, 574
BstMBI GATC 2 cut(s) 27, 250
BstMWI GCNNNNNNNGC 3 cut(s) 10, 42, 552
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 3 cut(s) 81, 119, 555
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 3 cut(s) 192, 212, 363
BstV1I GCAGC 5 cut(s) 157, 178, 198, 206, 226
BstV2I GAAGAC 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 1 cut(s) 45
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 243, 499
Cac8I GCNNGC 6 cut(s) 121, 234, 387, 399, 425, 553
CfoI GCGC 2 cut(s) 244, 267
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 26
CviAII CATG 4 cut(s) 78, 116, 552, 573
DdeI CTNAG 3 cut(s) 274, 285, 456
DinI GGCGCC 2 cut(s) 243, 266
DpnI GATC 2 cut(s) 29, 252
DpnII GATC 2 cut(s) 27, 250
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 20
Eco88I CYCGRG 2 cut(s) 380, 464
EcoRII CCWGG 1 cut(s) 139
EcoT22I ATGCAT 2 cut(s) 512, 548
EgeI GGCGCC 2 cut(s) 243, 266
EheI GGCGCC 2 cut(s) 243, 266
FaeI CATG 4 cut(s) 81, 119, 555, 576
FaiI YATR 9 cut(s) 75, 79, 117, 326, 365, 506, 508, 553, 574
FatI CATG 4 cut(s) 77, 115, 551, 572
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 5 cut(s) 171, 192, 195, 212, 215
Fsp4HI GCNGC 5 cut(s) 171, 192, 195, 212, 215
FspBI CTAG 4 cut(s) 174, 218, 233, 449
GlaI GCGC 2 cut(s) 243, 266
GluI GCNGC 5 cut(s) 171, 192, 195, 212, 215
HaeII RGCGCY 2 cut(s) 245, 268
HaeIII GGCC 1 cut(s) 45
HapII CCGG 1 cut(s) 527
HhaI GCGC 2 cut(s) 244, 267
Hin1I GRCGYC 2 cut(s) 242, 265
Hin1II CATG 4 cut(s) 81, 119, 555, 576
Hin6I GCGC 2 cut(s) 242, 265
HinP1I GCGC 2 cut(s) 242, 265
HinfI GANTC 1 cut(s) 489
HpaII CCGG 1 cut(s) 527
Hpy166II GTNNAC 1 cut(s) 446
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 1 cut(s) 380
Hpy8I GTNNAC 1 cut(s) 446
Hpy99I CGWCG 2 cut(s) 36, 44
HpyAV CCTTC 3 cut(s) 109, 368, 508
HpyCH4III ACNGT 2 cut(s) 443, 517
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 6 cut(s) 4, 194, 214, 510, 546, 551
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 42, 552
HpyF3I CTNAG 3 cut(s) 274, 285, 456
HpySE526I ACGT 1 cut(s) 7
Hsp92I GRCGYC 2 cut(s) 242, 265
Hsp92II CATG 4 cut(s) 81, 119, 555, 576
HspAI GCGC 2 cut(s) 242, 265
KasI GGCGCC 2 cut(s) 241, 264
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 27, 250
LpnPI CCDG 7 cut(s) 126, 153, 258, 265, 443, 520, 540
Lsp1109I GCAGC 5 cut(s) 157, 178, 198, 206, 226
LweI GCATC 3 cut(s) 320, 486, 519
MaeI CTAG 4 cut(s) 174, 218, 233, 449
MaeII ACGT 1 cut(s) 7
MalI GATC 2 cut(s) 29, 252
MbiI CCGCTC 1 cut(s) 13
MboI GATC 2 cut(s) 27, 250
MboII GAAGA 4 cut(s) 96, 361, 472, 493
MluCI AATT 2 cut(s) 291, 595
Mly113I GGCGCC 2 cut(s) 242, 265
MlyI GAGTC 1 cut(s) 498
MmeI TCCRAC 1 cut(s) 462
Mph1103I ATGCAT 2 cut(s) 512, 548
MseI TTAA 1 cut(s) 613
MslI CAYNNNNRTG 1 cut(s) 114
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 3 cut(s) 10, 42, 552
NarI GGCGCC 2 cut(s) 242, 265
NdeI CATATG 1 cut(s) 506
NdeII GATC 2 cut(s) 27, 250
NheI GCTAGC 1 cut(s) 232
NlaIII CATG 4 cut(s) 81, 119, 555, 576
NlaIV GGNNCC 3 cut(s) 21, 243, 266
NsiI ATGCAT 2 cut(s) 512, 548
NspI RCATGY 3 cut(s) 81, 119, 555
PaeI GCATGC 1 cut(s) 555
PaeR7I CTCGAG 2 cut(s) 380, 464
PciI ACATGT 1 cut(s) 77
PkrI GCNGC 5 cut(s) 172, 193, 196, 213, 216
PleI GAGTC 1 cut(s) 497
PluTI GGCGCC 2 cut(s) 245, 268
PpsI GAGTC 1 cut(s) 497
PscI ACATGT 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 21, 243, 266
PspPI GGNCC 1 cut(s) 20
PspXI VCTCGAGB 1 cut(s) 464
PstI CTGCAG 2 cut(s) 196, 216
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 613
SatI GCNGC 5 cut(s) 171, 192, 195, 212, 215
Sau3AI GATC 2 cut(s) 27, 250
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 498
ScrFI CCNGG 1 cut(s) 141
SetI ASST 8 cut(s) 10, 172, 199, 219, 308, 360, 387, 539
SfaNI GCATC 3 cut(s) 320, 486, 519
SfcI CTRYAG 3 cut(s) 192, 212, 363
SfoI GGCGCC 2 cut(s) 243, 266
Sfr274I CTCGAG 2 cut(s) 380, 464
SinI GGWCC 1 cut(s) 20
SlaI CTCGAG 2 cut(s) 380, 464
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 380, 464
SmoI CTYRAG 2 cut(s) 380, 464
SpeI ACTAGT 1 cut(s) 448
SphI GCATGC 1 cut(s) 555
Sse9I AATT 2 cut(s) 291, 595
SsiI CCGC 1 cut(s) 11
SspDI GGCGCC 2 cut(s) 241, 264
SspMI CTAG 4 cut(s) 174, 218, 233, 449
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 443, 517
TaiI ACGT 1 cut(s) 10
TaqI TCGA 4 cut(s) 26, 321, 381, 465
TasI AATT 2 cut(s) 291, 595
Tru1I TTAA 1 cut(s) 613
Tru9I TTAA 1 cut(s) 613
TscAI CASTG 2 cut(s) 243, 499
TseI GCWGC 5 cut(s) 170, 191, 194, 211, 214
TspDTI ATGAA 2 cut(s) 493, 547
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 2 cut(s) 243, 499
VpaK11BI GGWCC 1 cut(s) 20
XceI RCATGY 3 cut(s) 81, 119, 555
XhoI CTCGAG 2 cut(s) 380, 464
XspI CTAG 4 cut(s) 174, 218, 233, 449
Zsp2I ATGCAT 2 cut(s) 512, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.