No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 389 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 194, 442 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AclWI | GGATC | 1 cut(s) | 179 |
| AcuI | CTGAAG | 1 cut(s) | 398 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 194, 214, 442 |
| AgsI | TTSAA | 2 cut(s) | 344, 509 |
| AjiI | CACGTC | 1 cut(s) | 88 |
| AjnI | CCWGG | 1 cut(s) | 207 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 291, 323 |
| AluBI | AGCT | 3 cut(s) | 131, 137, 307 |
| AluI | AGCT | 3 cut(s) | 131, 137, 307 |
| AlwI | GGATC | 1 cut(s) | 179 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 367 |
| AoxI | GGCC | 1 cut(s) | 288 |
| ApeKI | GCWGC | 4 cut(s) | 355, 358, 361, 467 |
| AspLEI | GCGC | 2 cut(s) | 390, 435 |
| AsuHPI | GGTGA | 2 cut(s) | 229, 513 |
| BbvI | GCAGC | 4 cut(s) | 367, 370, 373, 454 |
| BccI | CCATC | 2 cut(s) | 101, 436 |
| BciT130I | CCWGG | 1 cut(s) | 209 |
| BclI | TGATCA | 2 cut(s) | 238, 406 |
| BfaI | CTAG | 2 cut(s) | 401, 422 |
| BfmI | CTRYAG | 1 cut(s) | 132 |
| BisI | GCNGC | 4 cut(s) | 356, 359, 362, 468 |
| BlsI | GCNGC | 4 cut(s) | 357, 360, 363, 469 |
| Bme1390I | CCNGG | 1 cut(s) | 209 |
| BmgBI | CACGTC | 1 cut(s) | 88 |
| BmrFI | CCNGG | 1 cut(s) | 209 |
| BmrI | ACTGGG | 1 cut(s) | 18 |
| BmsI | GCATC | 5 cut(s) | 12, 324, 363, 454, 479 |
| BmuI | ACTGGG | 1 cut(s) | 18 |
| BpmI | CTGGAG | 1 cut(s) | 389 |
| BsaBI | GATNNNNATC | 1 cut(s) | 446 |
| BsaJI | CCNNGG | 2 cut(s) | 188, 208 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 194, 214, 442 |
| Bse1I | ACTGG | 2 cut(s) | 13, 372 |
| Bse3DI | GCAATG | 1 cut(s) | 270 |
| Bse8I | GATNNNNATC | 1 cut(s) | 446 |
| BseBI | CCWGG | 1 cut(s) | 209 |
| BseDI | CCNNGG | 2 cut(s) | 188, 208 |
| BseGI | GGATG | 2 cut(s) | 339, 469 |
| BseJI | GATNNNNATC | 1 cut(s) | 446 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 194, 214, 442 |
| BseMI | GCAATG | 1 cut(s) | 270 |
| BseMII | CTCAG | 2 cut(s) | 9, 92 |
| BseNI | ACTGG | 2 cut(s) | 13, 372 |
| BseXI | GCAGC | 4 cut(s) | 367, 370, 373, 454 |
| BshFI | GGCC | 1 cut(s) | 290 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 194, 214, 442 |
| BsnI | GGCC | 1 cut(s) | 290 |
| Bsp143I | GATC | 4 cut(s) | 55, 184, 238, 406 |
| BspANI | GGCC | 1 cut(s) | 290 |
| BspCNI | CTCAG | 2 cut(s) | 10, 91 |
| BspPI | GGATC | 1 cut(s) | 179 |
| BsrDI | GCAATG | 1 cut(s) | 270 |
| BsrI | ACTGG | 2 cut(s) | 13, 372 |
| BssECI | CCNNGG | 2 cut(s) | 188, 208 |
| BssMI | GATC | 4 cut(s) | 55, 184, 238, 406 |
| BssT1I | CCWWGG | 1 cut(s) | 188 |
| Bst2UI | CCWGG | 1 cut(s) | 209 |
| Bst6I | CTCTTC | 2 cut(s) | 153, 276 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 204 |
| BstC8I | GCNNGC | 1 cut(s) | 437 |
| BstDEI | CTNAG | 2 cut(s) | 18, 78 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 212 |
| BstF5I | GGATG | 2 cut(s) | 339, 469 |
| BstHHI | GCGC | 2 cut(s) | 390, 435 |
| BstKTI | GATC | 4 cut(s) | 58, 187, 241, 409 |
| BstMBI | GATC | 4 cut(s) | 55, 184, 238, 406 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 204, 361 |
| BstNI | CCWGG | 1 cut(s) | 209 |
| BstSCI | CCNGG | 1 cut(s) | 207 |
| BstSFI | CTRYAG | 1 cut(s) | 132 |
| BstV1I | GCAGC | 4 cut(s) | 367, 370, 373, 454 |
| BsuRI | GGCC | 1 cut(s) | 290 |
| BtrI | CACGTC | 1 cut(s) | 88 |
| BtsCI | GGATG | 2 cut(s) | 339, 469 |
| BtsI | GCAGTG | 1 cut(s) | 144 |
| BtsIMutI | CAGTG | 1 cut(s) | 144 |
| Cac8I | GCNNGC | 1 cut(s) | 437 |
| CaiI | CAGNNNCTG | 1 cut(s) | 367 |
| CfoI | GCGC | 2 cut(s) | 390, 435 |
| CviAII | CATG | 2 cut(s) | 204, 483 |
| CviJI | RGCY | 6 cut(s) | 82, 131, 137, 290, 302, 307 |
| CviKI_1 | RGCY | 6 cut(s) | 82, 131, 137, 290, 302, 307 |
| DdeI | CTNAG | 2 cut(s) | 18, 78 |
| DpnI | GATC | 4 cut(s) | 57, 186, 240, 408 |
| DpnII | GATC | 4 cut(s) | 55, 184, 238, 406 |
| Eam1104I | CTCTTC | 2 cut(s) | 153, 276 |
| EarI | CTCTTC | 2 cut(s) | 153, 276 |
| Eco130I | CCWWGG | 1 cut(s) | 188 |
| Eco57I | CTGAAG | 1 cut(s) | 398 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 212 |
| EcoRII | CCWGG | 1 cut(s) | 207 |
| EcoT14I | CCWWGG | 1 cut(s) | 188 |
| ErhI | CCWWGG | 1 cut(s) | 188 |
| FaeI | CATG | 2 cut(s) | 207, 486 |
| FaiI | YATR | 7 cut(s) | 200, 205, 224, 231, 248, 264, 484 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 291, 323 |
| FatI | CATG | 2 cut(s) | 203, 482 |
| FbaI | TGATCA | 2 cut(s) | 238, 406 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 4 cut(s) | 356, 359, 362, 468 |
| FokI | GGATG | 2 cut(s) | 346, 476 |
| Fsp4HI | GCNGC | 4 cut(s) | 356, 359, 362, 468 |
| FspBI | CTAG | 2 cut(s) | 401, 422 |
| FspI | TGCGCA | 1 cut(s) | 389 |
| GlaI | GCGC | 2 cut(s) | 389, 434 |
| GluI | GCNGC | 4 cut(s) | 356, 359, 362, 468 |
| GsuI | CTGGAG | 1 cut(s) | 389 |
| HaeIII | GGCC | 1 cut(s) | 290 |
| HhaI | GCGC | 2 cut(s) | 390, 435 |
| Hin1II | CATG | 2 cut(s) | 207, 486 |
| Hin6I | GCGC | 2 cut(s) | 388, 433 |
| HinP1I | GCGC | 2 cut(s) | 388, 433 |
| HinfI | GANTC | 2 cut(s) | 340, 493 |
| HphI | GGTGA | 2 cut(s) | 229, 513 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188I | TCNGA | 1 cut(s) | 19 |
| Hpy188III | TCNNGA | 4 cut(s) | 170, 242, 344, 497 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| HpyAV | CCTTC | 1 cut(s) | 76 |
| HpyCH4IV | ACGT | 1 cut(s) | 87 |
| HpyCH4V | TGCA | 6 cut(s) | 104, 275, 337, 355, 416, 470 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 204, 361 |
| HpyF3I | CTNAG | 2 cut(s) | 18, 78 |
| HpySE526I | ACGT | 1 cut(s) | 87 |
| Hsp92II | CATG | 2 cut(s) | 207, 486 |
| HspAI | GCGC | 2 cut(s) | 388, 433 |
| Ksp22I | TGATCA | 2 cut(s) | 238, 406 |
| Kzo9I | GATC | 4 cut(s) | 55, 184, 238, 406 |
| LmnI | GCTCC | 3 cut(s) | 34, 311, 487 |
| Lsp1109I | GCAGC | 4 cut(s) | 367, 370, 373, 454 |
| LweI | GCATC | 5 cut(s) | 12, 324, 363, 454, 479 |
| MaeI | CTAG | 2 cut(s) | 401, 422 |
| MaeII | ACGT | 1 cut(s) | 87 |
| MaeIII | GTNAC | 1 cut(s) | 139 |
| MalI | GATC | 4 cut(s) | 57, 186, 240, 408 |
| MboI | GATC | 4 cut(s) | 55, 184, 238, 406 |
| MboII | GAAGA | 3 cut(s) | 103, 170, 293 |
| MfeI | CAATTG | 1 cut(s) | 38 |
| MluCI | AATT | 2 cut(s) | 38, 99 |
| MnlI | CCTC | 5 cut(s) | 6, 85, 109, 454, 507 |
| MseI | TTAA | 1 cut(s) | 531 |
| MspR9I | CCNGG | 1 cut(s) | 209 |
| MunI | CAATTG | 1 cut(s) | 38 |
| MvaI | CCWGG | 1 cut(s) | 209 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 204, 361 |
| NdeII | GATC | 4 cut(s) | 55, 184, 238, 406 |
| NlaIII | CATG | 2 cut(s) | 207, 486 |
| NmeAIII | GCCGAG | 1 cut(s) | 328 |
| NmuCI | GTSAC | 1 cut(s) | 139 |
| NsbI | TGCGCA | 1 cut(s) | 389 |
| PfeI | GAWTC | 2 cut(s) | 340, 493 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 194, 442 |
| PkrI | GCNGC | 4 cut(s) | 357, 360, 363, 469 |
| Psp6I | CCWGG | 1 cut(s) | 207 |
| PspGI | CCWGG | 1 cut(s) | 207 |
| PstNI | CAGNNNCTG | 1 cut(s) | 367 |
| SaqAI | TTAA | 1 cut(s) | 531 |
| SatI | GCNGC | 4 cut(s) | 356, 359, 362, 468 |
| Sau3AI | GATC | 4 cut(s) | 55, 184, 238, 406 |
| ScrFI | CCNGG | 1 cut(s) | 209 |
| SetI | ASST | 9 cut(s) | 17, 77, 90, 133, 139, 194, 219, 309, 526 |
| SfaNI | GCATC | 5 cut(s) | 12, 324, 363, 454, 479 |
| SfcI | CTRYAG | 1 cut(s) | 132 |
| Sse9I | AATT | 2 cut(s) | 38, 99 |
| SspMI | CTAG | 2 cut(s) | 401, 422 |
| StyD4I | CCNGG | 1 cut(s) | 207 |
| StyI | CCWWGG | 1 cut(s) | 188 |
| TaiI | ACGT | 1 cut(s) | 90 |
| TasI | AATT | 2 cut(s) | 38, 99 |
| TfiI | GAWTC | 2 cut(s) | 340, 493 |
| Tru1I | TTAA | 1 cut(s) | 531 |
| Tru9I | TTAA | 1 cut(s) | 531 |
| TscAI | CASTG | 1 cut(s) | 151 |
| TseFI | GTSAC | 1 cut(s) | 139 |
| TseI | GCWGC | 4 cut(s) | 355, 358, 361, 467 |
| Tsp45I | GTSAC | 1 cut(s) | 139 |
| TspDTI | ATGAA | 3 cut(s) | 35, 259, 312 |
| TspRI | CASTG | 1 cut(s) | 151 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 194, 442 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 212 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XspI | CTAG | 2 cut(s) | 401, 422 |