MD12G1151700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000512
Physical Location & Seq
Reverse (-)
23138076 .. 23138495
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1151700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGAAGGCTTTTCTGCAGCAACTACTCAACAAGATATTCACTAGCGTGTTGGATAATGACCTTGACCCATCCAACTTAGATTGGGCAACGATTATTCTGGCGTATTGTTTGTCAACGACAATTGGAATGGCTCTTGTACCCATCCAACTGCACTCCAAGCAGCTCCCTATATCCTTGTGCTTTCTCGGTCTCATAATCAGACTCTCCTTCTCAAGCATCTTCGTTAGCAAGTTCATTCAGTACTCCAAATGCTCACGAATCATAGTACATCTCGTCCATTATTTCGCTGTCTTTTTTGGGGTGATCGCCTTATTCATTTCCATCACAATCCCATTTCCCCTCTGGTTCAAGTTTACTGCCTCTGTCATCTATGCGGCCTTAGGCCTTGTAATACTATTTTGTCACCACTACTATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.83

Weight (kDa)

8.98

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 374
AfaI GTAC 3 cut(s) 138, 242, 267
AgsI TTSAA 1 cut(s) 349
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw26I GTCTC 1 cut(s) 194
AoxI GGCC 2 cut(s) 375, 382
ApeKI GCWGC 2 cut(s) 16, 160
AsuHPI GGTGA 2 cut(s) 313, 395
AxyI CCTNAGG 1 cut(s) 379
BbvI GCAGC 2 cut(s) 28, 172
BccI CCATC 3 cut(s) 76, 149, 329
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 14
BisI GCNGC 3 cut(s) 17, 161, 375
BlsI GCNGC 3 cut(s) 18, 162, 376
BmcAI AGTACT 1 cut(s) 242
BmsI GCATC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 194
Bse21I CCTNAGG 1 cut(s) 379
BseGI GGATG 2 cut(s) 68, 141
BseXI GCAGC 2 cut(s) 28, 172
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 2 cut(s) 377, 384
BsmAI GTCTC 1 cut(s) 194
BsnI GGCC 2 cut(s) 377, 384
Bso31I GGTCTC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 303
BspACI CCGC 1 cut(s) 374
BspANI GGCC 2 cut(s) 377, 384
BspMAI CTGCAG 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 194
BssMI GATC 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 76, 379
BstF5I GGATG 2 cut(s) 68, 141
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 1 cut(s) 303
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 28, 172
Bsu36I CCTNAGG 1 cut(s) 379
BsuRI GGCC 2 cut(s) 377, 384
BtsCI GGATG 2 cut(s) 68, 141
Csp6I GTAC 3 cut(s) 137, 241, 266
CviJI RGCY 5 cut(s) 8, 131, 163, 377, 384
CviKI_1 RGCY 5 cut(s) 8, 131, 163, 377, 384
CviQI GTAC 3 cut(s) 137, 241, 266
DdeI CTNAG 2 cut(s) 76, 379
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 194
Eco81I CCTNAGG 1 cut(s) 379
FaiI YATR 5 cut(s) 170, 194, 263, 372, 414
Fnu4HI GCNGC 3 cut(s) 17, 161, 375
FokI GGATG 2 cut(s) 55, 128
Fsp4HI GCNGC 3 cut(s) 17, 161, 375
FspBI CTAG 1 cut(s) 42
GluI GCNGC 3 cut(s) 17, 161, 375
HaeIII GGCC 2 cut(s) 377, 384
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 201, 258
HphI GGTGA 2 cut(s) 313, 395
Hpy166II GTNNAC 2 cut(s) 114, 354
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 255
Hpy8I GTNNAC 2 cut(s) 114, 354
HpyAV CCTTC 1 cut(s) 217
HpyCH4V TGCA 2 cut(s) 16, 151
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 76, 379
Kzo9I GATC 1 cut(s) 303
LmnI GCTCC 1 cut(s) 168
LpnPI CCDG 2 cut(s) 83, 328
Lsp1109I GCAGC 2 cut(s) 28, 172
LweI GCATC 1 cut(s) 225
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 401
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 1 cut(s) 211
MfeI CAATTG 1 cut(s) 120
MluCI AATT 1 cut(s) 120
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 3 cut(s) 30, 96, 169
MnlI CCTC 2 cut(s) 350, 370
MslI CAYNNNNRTG 1 cut(s) 44
MunI CAATTG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 401
OliI CACNNNNGTG 1 cut(s) 44
PceI AGGCCT 1 cut(s) 384
PfeI GAWTC 1 cut(s) 258
PkrI GCNGC 3 cut(s) 18, 162, 376
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PstI CTGCAG 1 cut(s) 18
RsaI GTAC 3 cut(s) 138, 242, 267
RsaNI GTAC 3 cut(s) 137, 241, 266
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 3 cut(s) 17, 161, 375
Sau3AI GATC 1 cut(s) 303
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 1 cut(s) 195
SetI ASST 2 cut(s) 63, 165
SfaNI GCATC 1 cut(s) 225
SfcI CTRYAG 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 1 cut(s) 120
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 1 cut(s) 374
SspMI CTAG 1 cut(s) 42
StuI AGGCCT 1 cut(s) 384
TaqII GACCGA 1 cut(s) 176
TasI AATT 1 cut(s) 120
TatI WGTACW 2 cut(s) 240, 265
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 1 cut(s) 258
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 16, 160
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 3 cut(s) 17, 223, 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 339
XspI CTAG 1 cut(s) 42
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.