FvH4_5g13230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000512
Physical Location & Seq
Reverse (-)
7483211 .. 7483825
615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13230.t1

Sequence Viewer

Length: 480 bp
ATGAGTTTTGCTGCTGCTATTACAAACCTGCTGCAGCTACTTCAGACACTTGGCCCATCTCATGGTCCTCCAACTCCTCCTGACCCAGAAGCAGTGGCGCCGCCTGATGATCAAGCAGTTCCAGGGCGCCAAAACTTAGACTGGGCTAAGATAATTGTGGTGTTTAGCTTGGCATCAGCAATCGACATAGCTCTCGTATCAGTCCAAGTTCGCTCCCAACTTCCTCTAGTTTTCTACTTTCTCGAGCTTGCTATCTTGCTTGCCTTTACTTGTTTATTCACAGGCAAGACAGTTCATTCCAACTCTCCACTAGTAGCTCAGGTGCTCGAGCGATTCGGCATCTTCTTCGGCGTCAGCGCCTTCTTCATTTCCATCACCATTCCGTTTCCGGCTGTCTGGTTCAAATGCCTAGCATGCTTCATCTACGTGGTCTCATGGCTTGTGATATTCTTTTGCAATTCCTATTCTCTGCTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.44

Weight (kDa)

6.01

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 36
AccB1I GGYRCC 2 cut(s) 97, 126
AccB7I CCANNNNNTGG 1 cut(s) 62
AciI CCGC 1 cut(s) 101
AcuI CTGAAG 1 cut(s) 26
AcyI GRCGYC 3 cut(s) 98, 127, 351
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 1 cut(s) 403
AhlI ACTAGT 1 cut(s) 310
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 5 cut(s) 37, 168, 191, 247, 317
AluI AGCT 5 cut(s) 37, 168, 191, 247, 317
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 436
Ama87I CYCGRG 2 cut(s) 242, 326
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 4 cut(s) 11, 14, 31, 34
AspLEI GCGC 3 cut(s) 100, 129, 359
AspS9I GGNCC 2 cut(s) 53, 65
AsuHPI GGTGA 1 cut(s) 367
AvaI CYCGRG 2 cut(s) 242, 326
AvaII GGWCC 1 cut(s) 65
BanI GGYRCC 2 cut(s) 97, 126
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 2 cut(s) 18, 46
BccI CCATC 2 cut(s) 64, 380
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 436
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 3 cut(s) 227, 311, 410
BfmI CTRYAG 1 cut(s) 32
BfoI RGCGCY 3 cut(s) 101, 130, 360
BfuAI ACCTGC 1 cut(s) 36
BisI GCNGC 5 cut(s) 12, 15, 32, 35, 101
BlsI GCNGC 5 cut(s) 13, 16, 33, 36, 102
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 2 cut(s) 242, 326
BmgT120I GGNCC 2 cut(s) 53, 65
BmiI GGNNCC 2 cut(s) 99, 128
BmrFI CCNGG 1 cut(s) 123
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 182, 348
BmuI ACTGGG 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 427
BsaHI GRCGYC 3 cut(s) 98, 127, 351
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 2 cut(s) 18, 46
BshFI GGCC 1 cut(s) 54
BshNI GGYRCC 2 cut(s) 97, 126
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 2 cut(s) 242, 326
BsiSI CCGG 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 436
BsnI GGCC 1 cut(s) 54
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 2 cut(s) 242, 326
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 1 cut(s) 109
BspACI CCGC 1 cut(s) 101
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 2 cut(s) 99, 128
BspMAI CTGCAG 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 36
BspT107I GGYRCC 2 cut(s) 97, 126
BspTNI GGTCTC 1 cut(s) 436
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 1 cut(s) 109
BssNI GRCGYC 3 cut(s) 98, 127, 351
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 292
BstACI GRCGYC 3 cut(s) 98, 127, 351
BstBAI YACGTR 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 249, 261, 415
BstDEI CTNAG 3 cut(s) 136, 147, 318
BstH2I RGCGCY 3 cut(s) 101, 130, 360
BstHHI GCGC 3 cut(s) 100, 129, 359
BstKTI GATC 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 18, 46
BsuRI GGCC 1 cut(s) 54
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 99
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 3 cut(s) 249, 261, 415
CfoI GCGC 3 cut(s) 100, 129, 359
Cfr13I GGNCC 2 cut(s) 53, 65
CseI GACGC 1 cut(s) 340
CviAII CATG 3 cut(s) 62, 414, 435
CviJI RGCY 9 cut(s) 37, 54, 146, 168, 191, 247, 317, 392, 439
CviKI_1 RGCY 9 cut(s) 37, 54, 146, 168, 191, 247, 317, 392, 439
DdeI CTNAG 3 cut(s) 136, 147, 318
DinI GGCGCC 2 cut(s) 99, 128
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 26
Eco88I CYCGRG 2 cut(s) 242, 326
EcoRII CCWGG 1 cut(s) 121
EgeI GGCGCC 2 cut(s) 99, 128
EheI GGCGCC 2 cut(s) 99, 128
FaeI CATG 3 cut(s) 65, 417, 438
FaiI YATR 4 cut(s) 63, 188, 415, 436
FatI CATG 3 cut(s) 61, 413, 434
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 5 cut(s) 12, 15, 32, 35, 101
Fsp4HI GCNGC 5 cut(s) 12, 15, 32, 35, 101
FspBI CTAG 3 cut(s) 227, 311, 410
GlaI GCGC 3 cut(s) 99, 128, 358
GluI GCNGC 5 cut(s) 12, 15, 32, 35, 101
HaeII RGCGCY 3 cut(s) 101, 130, 360
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 389
HgaI GACGC 1 cut(s) 340
HhaI GCGC 3 cut(s) 100, 129, 359
Hin1I GRCGYC 3 cut(s) 98, 127, 351
Hin1II CATG 3 cut(s) 65, 417, 438
Hin6I GCGC 3 cut(s) 98, 127, 357
HinP1I GCGC 3 cut(s) 98, 127, 357
HinfI GANTC 1 cut(s) 333
HpaII CCGG 1 cut(s) 389
HphI GGTGA 1 cut(s) 367
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 2 cut(s) 80, 242
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 34, 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 3 cut(s) 136, 147, 318
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 3 cut(s) 98, 127, 351
Hsp92II CATG 3 cut(s) 65, 417, 438
HspAI GCGC 3 cut(s) 98, 127, 357
KasI GGCGCC 2 cut(s) 97, 126
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 2 cut(s) 18, 46
LweI GCATC 2 cut(s) 182, 348
MaeI CTAG 3 cut(s) 227, 311, 410
MaeII ACGT 1 cut(s) 426
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 3 cut(s) 334, 337, 355
MhlI GDGCHC 1 cut(s) 327
MluCI AATT 2 cut(s) 153, 457
Mly113I GGCGCC 2 cut(s) 98, 127
MmeI TCCRAC 2 cut(s) 95, 324
MnlI CCTC 3 cut(s) 78, 87, 234
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 414
NarI GGCGCC 2 cut(s) 98, 127
NdeII GATC 1 cut(s) 109
NlaIII CATG 3 cut(s) 65, 417, 438
NlaIV GGNNCC 2 cut(s) 99, 128
NspI RCATGY 1 cut(s) 417
PaeI GCATGC 1 cut(s) 417
PaeR7I CTCGAG 2 cut(s) 242, 326
PfeI GAWTC 1 cut(s) 333
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 5 cut(s) 13, 16, 33, 36, 102
PluTI GGCGCC 2 cut(s) 101, 130
Ppu21I YACGTR 1 cut(s) 427
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 2 cut(s) 99, 128
PspPI GGNCC 2 cut(s) 53, 65
PspXI VCTCGAGB 1 cut(s) 326
PstI CTGCAG 1 cut(s) 36
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 5 cut(s) 12, 15, 32, 35, 101
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 2 cut(s) 53, 65
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 327
SetI ASST 8 cut(s) 30, 39, 170, 193, 249, 319, 324, 429
SfaNI GCATC 2 cut(s) 182, 348
SfcI CTRYAG 1 cut(s) 32
SfoI GGCGCC 2 cut(s) 99, 128
Sfr274I CTCGAG 2 cut(s) 242, 326
SinI GGWCC 1 cut(s) 65
SlaI CTCGAG 2 cut(s) 242, 326
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 2 cut(s) 242, 326
SmoI CTYRAG 2 cut(s) 242, 326
SpeI ACTAGT 1 cut(s) 310
SphI GCATGC 1 cut(s) 417
Sse9I AATT 2 cut(s) 153, 457
SsiI CCGC 1 cut(s) 101
SspDI GGCGCC 2 cut(s) 97, 126
SspMI CTAG 3 cut(s) 227, 311, 410
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 429
TaqI TCGA 3 cut(s) 183, 243, 327
TasI AATT 2 cut(s) 153, 457
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 99
TseI GCWGC 4 cut(s) 11, 14, 31, 34
TspDTI ATGAA 3 cut(s) 284, 355, 409
TspGWI ACGGA 1 cut(s) 372
TspRI CASTG 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 65
XceI RCATGY 1 cut(s) 417
XhoI CTCGAG 2 cut(s) 242, 326
XspI CTAG 3 cut(s) 227, 311, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.