MD03G1009100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000512
Physical Location & Seq
Reverse (-)
709245 .. 709724
480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1009100.v1.1.491

Sequence Viewer

Length: 480 bp
ATGAAGGCATTTGTGCAGCAACTAGTCAACAAAATCTTCACTAGCGCGTTGGATAATGACCTAGAAACAACTGCACCCGCCGAACCACCAGCAGCAGCAGCTGCCGCCAACTCTAGGCAGCCTAGGAAGCACAACTTAGATTGGGCAACGATTATTCTGGCGTATTGCTTGTCAACGGCAATTGGAATGGCTCTCGTACCCATCCAACTGGATTCCAAGCAGCTCCCTCTAACCTTCTGCTTTCTCGGTCTCGCAATCACATTCTCTTTTTCTAGCATCTTGGTTAGCAAGTCCATTCAGAACTCCAAATGCCCACGAATCTTAGTTCATCTGTTCCATTATTTCGGTGTCTTCTTTGGAGTGACTGCCTTCTTCGTTTCCATCACAATCCCTTTTCCTCTCTGGTTCAAGTGTACTGCATCTGTCATCTATGTGGCCTCAGGCCTTGTAATACTGTTTTGTCACCACTACTATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.61

Weight (kDa)

9.06

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 47
AciI CCGC 2 cut(s) 78, 105
AfaI GTAC 2 cut(s) 198, 415
AfiI CCNNNNNNNGG 1 cut(s) 114
AgsI TTSAA 1 cut(s) 409
AhlI ACTAGT 1 cut(s) 22
AluBI AGCT 2 cut(s) 101, 223
AluI AGCT 2 cut(s) 101, 223
Alw26I GTCTC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 101
AoxI GGCC 2 cut(s) 435, 442
ApeKI GCWGC 7 cut(s) 16, 92, 95, 98, 101, 118, 220
AspA2I CCTAGG 1 cut(s) 122
AspLEI GCGC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 455
AvrII CCTAGG 1 cut(s) 122
AxyI CCTNAGG 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 7 cut(s) 28, 88, 104, 107, 110, 130, 232
BccI CCATC 2 cut(s) 209, 389
BceAI ACGGC 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 254
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 6 cut(s) 23, 42, 62, 114, 123, 273
BisI GCNGC 8 cut(s) 17, 93, 96, 99, 102, 105, 119, 221
BlnI CCTAGG 1 cut(s) 122
BlsI GCNGC 8 cut(s) 18, 94, 97, 100, 103, 106, 120, 222
BmsI GCATC 2 cut(s) 285, 428
BpiI GAAGAC 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 213
Bse21I CCTNAGG 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 1 cut(s) 213
BseXI GCAGC 7 cut(s) 28, 88, 104, 107, 110, 130, 232
BsgI GTGCAG 2 cut(s) 35, 57
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 437, 444
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 254
BsnI GGCC 2 cut(s) 437, 444
Bso31I GGTCTC 1 cut(s) 254
BspACI CCGC 2 cut(s) 78, 105
BspANI GGCC 2 cut(s) 437, 444
BspCNI CTCAG 1 cut(s) 452
BspFNI CGCG 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 1 cut(s) 213
BssECI CCNNGG 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 101
BstDEI CTNAG 3 cut(s) 136, 322, 439
BstF5I GGATG 1 cut(s) 201
BstFNI CGCG 1 cut(s) 47
BstHHI GCGC 1 cut(s) 47
BstMAI GTCTC 1 cut(s) 254
BstMWI GCNNNNNNNGC 4 cut(s) 98, 101, 104, 127
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 7 cut(s) 28, 88, 104, 107, 110, 130, 232
BstV2I GAAGAC 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 208
Bsu36I CCTNAGG 1 cut(s) 439
BsuRI GGCC 2 cut(s) 437, 444
BtsCI GGATG 1 cut(s) 201
CaiI CAGNNNCTG 1 cut(s) 101
CfoI GCGC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 197, 414
CviJI RGCY 6 cut(s) 101, 121, 191, 223, 437, 444
CviKI_1 RGCY 6 cut(s) 101, 121, 191, 223, 437, 444
CviQI GTAC 2 cut(s) 197, 414
DdeI CTNAG 3 cut(s) 136, 322, 439
Eco130I CCWWGG 1 cut(s) 122
Eco147I AGGCCT 1 cut(s) 444
Eco31I GGTCTC 1 cut(s) 254
Eco81I CCTNAGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaiI YATR 2 cut(s) 432, 474
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 8 cut(s) 17, 93, 96, 99, 102, 105, 119, 221
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 8 cut(s) 17, 93, 96, 99, 102, 105, 119, 221
FspBI CTAG 6 cut(s) 23, 42, 62, 114, 123, 273
GlaI GCGC 1 cut(s) 46
GluI GCNGC 8 cut(s) 17, 93, 96, 99, 102, 105, 119, 221
HaeIII GGCC 2 cut(s) 437, 444
HhaI GCGC 1 cut(s) 47
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 2 cut(s) 28, 174
HindII GTYRAC 2 cut(s) 28, 174
HinfI GANTC 2 cut(s) 212, 318
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 3 cut(s) 28, 174, 414
Hpy188I TCNGA 1 cut(s) 300
Hpy8I GTNNAC 3 cut(s) 28, 174, 414
HpyAV CCTTC 2 cut(s) 244, 379
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4V TGCA 3 cut(s) 16, 74, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 101, 104, 127
HpyF3I CTNAG 3 cut(s) 136, 322, 439
HspAI GCGC 1 cut(s) 45
LmnI GCTCC 1 cut(s) 228
LpnPI CCDG 5 cut(s) 102, 143, 194, 388, 426
Lsp1109I GCAGC 7 cut(s) 28, 88, 104, 107, 110, 130, 232
LweI GCATC 2 cut(s) 285, 428
MaeI CTAG 6 cut(s) 23, 42, 62, 114, 123, 273
MaeIII GTNAC 2 cut(s) 361, 461
MboII GAAGA 3 cut(s) 28, 343, 364
MfeI CAATTG 1 cut(s) 180
MluCI AATT 1 cut(s) 180
MmeI TCCRAC 2 cut(s) 30, 229
MnlI CCTC 3 cut(s) 237, 408, 448
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 101
MunI CAATTG 1 cut(s) 180
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 4 cut(s) 98, 101, 104, 127
NmuCI GTSAC 2 cut(s) 361, 461
PceI AGGCCT 1 cut(s) 444
PfeI GAWTC 2 cut(s) 212, 318
PkrI GCNGC 8 cut(s) 18, 94, 97, 100, 103, 106, 120, 222
PstNI CAGNNNCTG 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 2 cut(s) 198, 415
RsaNI GTAC 2 cut(s) 197, 414
RseI CAYNNNNRTG 1 cut(s) 431
SatI GCNGC 8 cut(s) 17, 93, 96, 99, 102, 105, 119, 221
SetI ASST 4 cut(s) 63, 103, 225, 236
SfaNI GCATC 2 cut(s) 285, 428
SmiMI CAYNNNNRTG 1 cut(s) 431
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 1 cut(s) 180
SseBI AGGCCT 1 cut(s) 444
SsiI CCGC 2 cut(s) 78, 105
SspMI CTAG 6 cut(s) 23, 42, 62, 114, 123, 273
StuI AGGCCT 1 cut(s) 444
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 456
TaqII GACCGA 1 cut(s) 236
TasI AATT 1 cut(s) 180
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 212, 318
TseFI GTSAC 2 cut(s) 361, 461
TseI GCWGC 7 cut(s) 16, 92, 95, 98, 101, 118, 220
Tsp45I GTSAC 2 cut(s) 361, 461
TspDTI ATGAA 2 cut(s) 17, 317
XmaJI CCTAGG 1 cut(s) 122
XspI CTAG 6 cut(s) 23, 42, 62, 114, 123, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.