RLG00000021010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000512
Physical Location & Seq
Reverse (-)
71036601 .. 71040540
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021010

Sequence Viewer

Length: 741 bp
ATGGCTGCAAAAGATGGGGAGCTGAGGGTCTTCATAGTTTCTGGAGAGGTTTCTGGAGACACCACTGGCTCTCGTCTGATGGCATCTCTAAAGAAGCTCTCCTGTTCCAGTCCGCTTTTCTGGTCCTTATCAAAATCCCCGTGGAAGTCTCTGAATGCGGTCGTTGTATTGCGCCTAGTCGATGGCGTCCTGGATCGGTGGCATGGCGTCGTGGTTTTTATGGTTTTGTTTCGACGGCAGCACGAGTCGTATGGGTGCTGGGCTCCAGGCGATATGGCCTTTACTTCTGCTATCAAAAAAATGCTCCGGCTACTTCAGAAACTTAGTCCATTTGATGCTCCTCCCGACCCAGAAGCAGTGCGGCCTGATCAAGCACCAAAGCACCAAAACTTGGACTGGGCTAAGATACTTGTGGTATTCAGCTTAGCATCAGCAATTGAGATAGCTCTCCTATCCGTCCAGATTCACTCCCAGAAGCTCCCTATAATCTTTTACTTTCTCGAGCTCGCTCTCCTGCTTGCCTTTTCTTGTTTCTTCACCAGCAAGTCAGTTCATTCCAACTCTCCGCTAGTCGCTCGAGTTCTCGACCGATTTGGCATATTTTTTGGAGTGACTGCATTCTTCATATCCATCACTATTCCATTTCCAGCTCTATGGTTCAAATGTACTGCGTGCTTCGTCTACGCAGTTTCTTGGCTTGCAATCTTGTTTTGCAATTCTTCATCATCCTCTGCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

27.32

Weight (kDa)

9.32

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000512)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13130 FvH4_5g13140 FvH4_5g13141 FvH4_5g13170 FvH4_5g13220 FvH4_5g13221 FvH4_5g13222 FvH4_5g13223 FvH4_5g13230
malus_domestica MD03G1009100.v1.1 MD03G1009500.v1.1 MD03G1009600.v1.1 MD03G1010100.v1.1 MD03G1011400.v1.1 MD11G1013400.v1.1 MD11G1013900.v1.1 MD12G1151700.v1.1 MD12G1151900.v1.1 MD14G1141800.v1.1
prunus_persica Prupe.1G109400_v2.0.a1 Prupe.3G028900_v2.0.a1 Prupe.6G011200_v2.0.a1 Prupe.6G011700_v2.0.a1 Prupe.6G011800_v2.0.a1
pyrus_communis pycom03g01000 pycom07g22580 pycom11g00910 pycom11g00930
rosa_chinensis RchiOBHm_Chr7g0240781 RchiOBHm_Chr7g0241491
rosa_laevigata RLG00000000572 RLG00000005009 RLG00000014262 RLG00000021010 RLG00000021011 RLG00000026751
rosa_multiflora Rmu_sc0000215.1_g000008 Rmu_sc0001275.1_g000010 Rmu_sc0002761.1_g000010 Rmu_sc0003825.1_g000034 Rmu_sc0004712.1_g000019 Rmu_sc0004712.1_g000020 Rmu_sc0004712.1_g000025 Rmu_sc0004712.1_g000028 Rmu_sc0004712.1_g000030 Rmu_sc0004821.1_g000003 Rmu_sc0005888.1_g000011 Rmu_sc0007533.1_g000008 Rmu_sc0007533.1_g000010 Rmu_sc0008698.1_g000006 Rmu_sc0010322.1_g000017 Rmu_sc0010483.1_g000002 Rmu_sc0013122.1_g000003
rosa_roxburghii Rroxscaffold_176G00431730 Rroxscaffold_3G00219000 Rroxscaffold_3G00269990 Rroxscaffold_3G00270040 Rroxscaffold_3G00270050 Rroxscaffold_3G00270060 Rroxscaffold_4G00283100 Rroxscaffold_6G00430000
rosa_rugosa Rorug01G0382000 Rorug02G0463100 Rorug02G0644300 Rorug06G0011800 Rorug06G0461600 Rorug07G0344600 Rorug07G0344700
rosa_samantha Rh2AG527500 Rh2AG527800 Rh2CG512000 Rh2CG512200 Rh2DG550800 Rh2DG551000 Rh3CG048300 Rh3DG049200 Rh7AG062500 Rh7AG062700 Rh7AG062800 Rh7BG062900 Rh7BG063000 Rh7BG063100 Rh7BG469800 Rh7DG062500 Rh7DG062800 Rh7DG063400 Rh7DG063500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 391
AccI GTMKAC 1 cut(s) 681
AciI CCGC 4 cut(s) 113, 158, 361, 566
AclWI GGATC 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 299
AcyI GRCGYC 2 cut(s) 186, 207
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 1 cut(s) 391
AgsI TTSAA 1 cut(s) 661
AjnI CCWGG 2 cut(s) 189, 265
AluBI AGCT 7 cut(s) 22, 97, 423, 446, 478, 505, 650
AluI AGCT 7 cut(s) 22, 97, 423, 446, 478, 505, 650
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 2 cut(s) 51, 153
AlwI GGATC 1 cut(s) 201
Ama87I CYCGRG 2 cut(s) 500, 576
AoxI GGCC 2 cut(s) 276, 362
ApeKI GCWGC 2 cut(s) 5, 238
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 529
AvaI CYCGRG 2 cut(s) 500, 576
AvaII GGWCC 1 cut(s) 123
BanII GRGCYC 2 cut(s) 265, 507
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 507
BbvCI CCTCAGC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 250
BccI CCATC 4 cut(s) 8, 73, 176, 638
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 2 cut(s) 191, 267
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 51, 153
BfaI CTAG 2 cut(s) 176, 569
BisI GCNGC 3 cut(s) 6, 239, 362
BlpI GCTNAGC 1 cut(s) 424
BlsI GCNGC 3 cut(s) 7, 240, 363
Bme1390I CCNGG 2 cut(s) 191, 267
Bme18I GGWCC 1 cut(s) 123
BmeT110I CYCGRG 2 cut(s) 500, 576
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 2 cut(s) 191, 267
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 92, 325, 437
BmuI ACTGGG 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 3 cut(s) 63, 75, 249
Bpu10I CCTNAGC 1 cut(s) 23
Bpu1102I GCTNAGC 1 cut(s) 424
BsaHI GRCGYC 2 cut(s) 186, 207
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 391
Bse1I ACTGG 3 cut(s) 70, 108, 401
BseBI CCWGG 2 cut(s) 191, 267
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 1 cut(s) 725
BseLI CCNNNNNNNGG 1 cut(s) 391
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 3 cut(s) 70, 108, 401
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 250
BseYI CCCAGC 1 cut(s) 258
Bsh1285I CGRYCG 2 cut(s) 162, 589
BshFI GGCC 2 cut(s) 278, 364
BsiEI CGRYCG 2 cut(s) 162, 589
BsiHKAI GWGCWC 1 cut(s) 507
BsiHKCI CYCGRG 2 cut(s) 500, 576
BsiSI CCGG 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 391
BsmAI GTCTC 2 cut(s) 51, 153
BsmI GAATGC 2 cut(s) 160, 617
BsnI GGCC 2 cut(s) 278, 364
BsoBI CYCGRG 2 cut(s) 500, 576
Bsp1286I GDGCHC 2 cut(s) 265, 507
Bsp143I GATC 2 cut(s) 193, 367
Bsp1720I GCTNAGC 1 cut(s) 424
BspACI CCGC 4 cut(s) 113, 158, 361, 566
BspANI GGCC 2 cut(s) 278, 364
BspCNI CTCAG 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 1 cut(s) 201
BsrI ACTGG 3 cut(s) 70, 108, 401
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 193, 367
BssNI GRCGYC 2 cut(s) 186, 207
BssSI CACGAG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 2 cut(s) 191, 267
BstACI GRCGYC 2 cut(s) 186, 207
BstC8I GCNNGC 4 cut(s) 507, 519, 673, 699
BstDEI CTNAG 4 cut(s) 23, 323, 402, 424
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 2 cut(s) 196, 370
BstMAI GTCTC 2 cut(s) 51, 153
BstMBI GATC 2 cut(s) 193, 367
BstMCI CGRYCG 2 cut(s) 162, 589
BstNI CCWGG 2 cut(s) 191, 267
BstSCI CCNGG 2 cut(s) 189, 265
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 654
BsuRI GGCC 2 cut(s) 278, 364
BtgI CCRYGG 1 cut(s) 140
BtsCI GGATG 1 cut(s) 725
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 2 cut(s) 63, 363
Cac8I GCNNGC 4 cut(s) 507, 519, 673, 699
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 123
CseI GACGC 2 cut(s) 175, 196
Csp6I GTAC 1 cut(s) 666
CviAII CATG 1 cut(s) 203
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 4 cut(s) 23, 323, 402, 424
DpnI GATC 2 cut(s) 195, 369
DpnII GATC 2 cut(s) 193, 367
Ecl136II GAGCTC 1 cut(s) 505
Eco24I GRGCYC 2 cut(s) 265, 507
Eco47I GGWCC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 299
Eco88I CYCGRG 2 cut(s) 500, 576
EcoICRI GAGCTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 189, 265
EcoT38I GRGCYC 2 cut(s) 265, 507
FaeI CATG 1 cut(s) 206
FaiI YATR 9 cut(s) 35, 204, 221, 252, 275, 485, 599, 626, 655
FatI CATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 3 cut(s) 6, 239, 362
FokI GGATG 1 cut(s) 712
FriOI GRGCYC 2 cut(s) 265, 507
Fsp4HI GCNGC 3 cut(s) 6, 239, 362
FspBI CTAG 2 cut(s) 176, 569
GlaI GCGC 1 cut(s) 173
GluI GCNGC 3 cut(s) 6, 239, 362
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 3 cut(s) 63, 75, 249
HaeIII GGCC 2 cut(s) 278, 364
HapII CCGG 1 cut(s) 307
HgaI GACGC 2 cut(s) 175, 196
HhaI GCGC 1 cut(s) 174
Hin1I GRCGYC 2 cut(s) 186, 207
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 2 cut(s) 245, 463
HpaII CCGG 1 cut(s) 307
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 1 cut(s) 682
Hpy188I TCNGA 3 cut(s) 78, 153, 318
Hpy188III TCNNGA 6 cut(s) 42, 54, 344, 460, 500, 584
Hpy8I GTNNAC 1 cut(s) 682
Hpy99I CGWCG 2 cut(s) 212, 237
HpyCH4V TGCA 4 cut(s) 8, 617, 701, 714
HpyF3I CTNAG 4 cut(s) 23, 323, 402, 424
Hsp92I GRCGYC 2 cut(s) 186, 207
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 2 cut(s) 193, 367
LmnI GCTCC 5 cut(s) 19, 268, 309, 343, 483
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 3 cut(s) 92, 325, 437
MaeI CTAG 2 cut(s) 176, 569
MaeIII GTNAC 1 cut(s) 610
MalI GATC 2 cut(s) 195, 369
MboI GATC 2 cut(s) 193, 367
MboII GAAGA 4 cut(s) 22, 526, 613, 711
MfeI CAATTG 1 cut(s) 435
MhlI GDGCHC 2 cut(s) 265, 507
MluCI AATT 2 cut(s) 435, 715
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 4 cut(s) 18, 40, 351, 739
MspI CCGG 1 cut(s) 307
MspR9I CCNGG 2 cut(s) 191, 267
MunI CAATTG 1 cut(s) 435
Mva1269I GAATGC 2 cut(s) 160, 617
MvaI CCWGG 2 cut(s) 191, 267
NdeII GATC 2 cut(s) 193, 367
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 610
PaeR7I CTCGAG 2 cut(s) 500, 576
PctI GAATGC 2 cut(s) 160, 617
PfeI GAWTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 3 cut(s) 7, 240, 363
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 189, 265
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 2 cut(s) 189, 265
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 123
PspXI VCTCGAGB 1 cut(s) 576
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
SacI GAGCTC 1 cut(s) 507
SatI GCNGC 3 cut(s) 6, 239, 362
Sau3AI GATC 2 cut(s) 193, 367
Sau96I GGNCC 1 cut(s) 123
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 191, 267
SduI GDGCHC 2 cut(s) 265, 507
SetI ASST 8 cut(s) 24, 51, 99, 425, 448, 480, 507, 652
SfaNI GCATC 3 cut(s) 92, 325, 437
Sfr274I CTCGAG 2 cut(s) 500, 576
SinI GGWCC 1 cut(s) 123
SlaI CTCGAG 2 cut(s) 500, 576
SmlI CTYRAG 2 cut(s) 500, 576
SmoI CTYRAG 2 cut(s) 500, 576
Sse9I AATT 2 cut(s) 435, 715
SsiI CCGC 4 cut(s) 113, 158, 361, 566
SspMI CTAG 2 cut(s) 176, 569
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 189, 265
TaqI TCGA 5 cut(s) 180, 232, 501, 577, 585
TaqII GACCGA 1 cut(s) 603
TasI AATT 2 cut(s) 435, 715
TatI WGTACW 1 cut(s) 665
TauI GCSGC 1 cut(s) 364
TfiI GAWTC 1 cut(s) 463
TscAI CASTG 2 cut(s) 70, 363
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 2 cut(s) 5, 238
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 4 cut(s) 22, 542, 613, 711
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 2 cut(s) 70, 363
Van91I CCANNNNNTGG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 123
XhoI CTCGAG 2 cut(s) 500, 576
XmiI GTMKAC 1 cut(s) 681
XspI CTAG 2 cut(s) 176, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.