MD05G1050700.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000400
Physical Location & Seq
Forward (+)
8797786 .. 8799884
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050700.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGATGCCGCTGTAGGCATCATTTTGTCCCCGGCTCTGCAGGTTCTTTTCGACAGACTGGCATCCCCAGTCCTACAAGGGCTTGCTGATATCCTGGGTTTCAATTTTGACATCTTCCAAAGCCTGCAGCATGCACTGGTGCGGGCTCAAGCTACTCTTGAAGATGCAGAAGTGCAACAATTCACCAACAAAACTGTCAGACTCTGGCTGGTGGATCTCAAGAATGCAGTTTGTGATGCTGAGGATCTTCTCGACGTCTTTACTGTTAAACAAACTGCCATGATTGACCAAGACTTTGGTAAGCAGACAGTAGAAAGCTATACATTTCTTACTGATAAAGTCAGGAACATACTCCAGAAGCTAGAAGTAACTGTAGCTGAAGGATCTTCTAAGTTGAAGATTAGAGAGCCTACTCAGCCAAGGTCAGATCGACAATCGGACAAAAGGGAGACTAGCTCTTTCATCGACTTGAGGATATATGGAAGAGATGATGACAAAGAGACGTTAGTCCAACTGTTGATGTCTTCTCAAACTGTTTACCAGGATGGATATACGTATGCATCGTGTATTCCCATCATCGGTATTGGAGGAATTGTCATGGCTGGTCTTGGTTTGAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

208

Amino Acids

23.02

Weight (kDa)

4.77

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 92 1.3e-15 Rx N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 31
AciI CCGC 2 cut(s) 9, 142
AclWI GGATC 3 cut(s) 222, 252, 391
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 255
AfiI CCNNNNNNNGG 1 cut(s) 578
AgsI TTSAA 4 cut(s) 103, 161, 397, 616
AjnI CCWGG 2 cut(s) 93, 540
AluBI AGCT 5 cut(s) 152, 318, 361, 377, 456
AluI AGCT 5 cut(s) 152, 318, 361, 377, 456
Alw26I GTCTC 2 cut(s) 443, 494
AlwI GGATC 3 cut(s) 222, 252, 391
AlwNI CAGNNNCTG 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 127
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 175
BanII GRGCYC 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 516
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 539, 581
BciT130I CCWGG 2 cut(s) 95, 542
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 443, 494
BfaI CTAG 2 cut(s) 362, 453
BfmI CTRYAG 4 cut(s) 12, 38, 125, 372
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 2 cut(s) 9, 128
BlsI GCNGC 2 cut(s) 10, 129
Bme1390I CCNGG 3 cut(s) 32, 95, 542
BmrFI CCNGG 3 cut(s) 32, 95, 542
BmrI ACTGGG 1 cut(s) 62
BmsI GCATC 5 cut(s) 27, 71, 154, 226, 569
BmuI ACTGGG 1 cut(s) 62
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 132, 203, 490
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 3 cut(s) 30, 94, 419
BsaXI ACNNNNNCTCC 2 cut(s) 579, 609
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse1I ACTGG 3 cut(s) 63, 68, 141
BseBI CCWGG 2 cut(s) 95, 542
BseDI CCNNGG 3 cut(s) 30, 94, 419
BseGI GGATG 3 cut(s) 10, 62, 550
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMII CTCAG 2 cut(s) 231, 428
BseNI ACTGG 3 cut(s) 63, 68, 141
BseXI GCAGC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 578
BsmAI GTCTC 2 cut(s) 443, 494
BsmBI CGTCTC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 13
BsmI GAATGC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 4 cut(s) 214, 244, 383, 427
BspACI CCGC 2 cut(s) 9, 142
BspCNI CTCAG 2 cut(s) 232, 427
BspMAI CTGCAG 2 cut(s) 42, 129
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 3 cut(s) 222, 252, 391
BsrI ACTGG 3 cut(s) 63, 68, 141
BssECI CCNNGG 3 cut(s) 30, 94, 419
BssMI GATC 4 cut(s) 214, 244, 383, 427
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 1 cut(s) 419
Bst2UI CCWGG 2 cut(s) 95, 542
Bst4CI ACNGT 6 cut(s) 196, 265, 310, 373, 516, 535
Bst6I CTCTTC 1 cut(s) 478
BstACI GRCGYC 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 555
BstC8I GCNNGC 4 cut(s) 84, 125, 132, 144
BstDEI CTNAG 3 cut(s) 240, 390, 414
BstF5I GGATG 3 cut(s) 10, 62, 550
BstKTI GATC 4 cut(s) 217, 247, 386, 430
BstMAI GTCTC 2 cut(s) 443, 494
BstMBI GATC 4 cut(s) 214, 244, 383, 427
BstMWI GCNNNNNNNGC 1 cut(s) 415
BstNI CCWGG 2 cut(s) 95, 542
BstNSI RCATGY 1 cut(s) 134
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 3 cut(s) 30, 93, 540
BstSFI CTRYAG 4 cut(s) 12, 38, 125, 372
BstSNI TACGTA 1 cut(s) 555
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 3 cut(s) 214, 244, 383
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 3 cut(s) 214, 244, 383
BtsCI GGATG 3 cut(s) 10, 62, 550
BtsIMutI CAGTG 1 cut(s) 134
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 4 cut(s) 84, 125, 132, 144
CaiI CAGNNNCTG 1 cut(s) 204
CviAII CATG 4 cut(s) 131, 280, 598, 621
DdeI CTNAG 3 cut(s) 240, 390, 414
DpnI GATC 4 cut(s) 216, 246, 385, 429
DpnII GATC 4 cut(s) 214, 244, 383, 427
Eam1104I CTCTTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 478
Eco105I TACGTA 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 93, 540
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 419
Esp3I CGTCTC 1 cut(s) 494
FaeI CATG 4 cut(s) 134, 283, 601, 624
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 1 cut(s) 13
FatI CATG 4 cut(s) 130, 279, 597, 620
FauI CCCGC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 9, 128
FokI GGATG 3 cut(s) 17, 49, 557
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 2 cut(s) 9, 128
FspBI CTAG 2 cut(s) 362, 453
GluI GCNGC 2 cut(s) 9, 128
GsuI CTGGAG 1 cut(s) 338
HapII CCGG 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 4 cut(s) 134, 283, 601, 624
HinfI GANTC 1 cut(s) 201
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 200, 427, 439
Hpy188III TCNNGA 5 cut(s) 158, 220, 251, 343, 355
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 374
HpyCH4III ACNGT 6 cut(s) 196, 265, 310, 373, 516, 535
HpyCH4IV ACGT 3 cut(s) 255, 503, 554
HpyCH4V TGCA 7 cut(s) 40, 127, 134, 167, 175, 227, 560
HpyF10VI GCNNNNNNNGC 1 cut(s) 415
HpyF3I CTNAG 3 cut(s) 240, 390, 414
HpySE526I ACGT 3 cut(s) 255, 503, 554
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 4 cut(s) 134, 283, 601, 624
Kzo9I GATC 4 cut(s) 214, 244, 383, 427
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 5 cut(s) 27, 71, 154, 226, 569
MaeI CTAG 2 cut(s) 362, 453
MaeII ACGT 3 cut(s) 255, 503, 554
MaeIII GTNAC 1 cut(s) 367
MalI GATC 4 cut(s) 216, 246, 385, 429
MboI GATC 4 cut(s) 214, 244, 383, 427
MboII GAAGA 7 cut(s) 106, 173, 239, 378, 409, 495, 516
MflI RGATCY 3 cut(s) 214, 244, 383
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 3 cut(s) 103, 179, 591
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 3 cut(s) 235, 465, 581
Mph1103I ATGCAT 1 cut(s) 562
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 32, 95, 542
Mva1269I GAATGC 1 cut(s) 229
MvaI CCWGG 2 cut(s) 95, 542
MwoI GCNNNNNNNGC 1 cut(s) 415
NciI CCSGG 1 cut(s) 32
NdeII GATC 4 cut(s) 214, 244, 383, 427
NlaIII CATG 4 cut(s) 134, 283, 601, 624
NsiI ATGCAT 1 cut(s) 562
NspI RCATGY 1 cut(s) 134
PaeI GCATGC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PctI GAATGC 1 cut(s) 229
PkrI GCNGC 2 cut(s) 10, 129
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
Ppu21I YACGTR 1 cut(s) 555
PshAI GACNNNNGTC 1 cut(s) 506
Psp6I CCWGG 2 cut(s) 93, 540
PspGI CCWGG 2 cut(s) 93, 540
PstI CTGCAG 2 cut(s) 42, 129
PstNI CAGNNNCTG 1 cut(s) 204
PsuI RGATCY 3 cut(s) 214, 244, 383
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 9, 128
Sau3AI GATC 4 cut(s) 214, 244, 383, 427
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 3 cut(s) 32, 95, 542
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 5 cut(s) 27, 71, 154, 226, 569
SfcI CTRYAG 4 cut(s) 12, 38, 125, 372
SmlI CTYRAG 3 cut(s) 147, 218, 469
SmoI CTYRAG 3 cut(s) 147, 218, 469
SnaBI TACGTA 1 cut(s) 555
SphI GCATGC 1 cut(s) 134
Sse9I AATT 3 cut(s) 103, 179, 591
SsiI CCGC 2 cut(s) 9, 142
SspMI CTAG 2 cut(s) 362, 453
StyD4I CCNGG 3 cut(s) 30, 93, 540
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 6 cut(s) 196, 265, 310, 373, 516, 535
TaiI ACGT 3 cut(s) 258, 506, 557
TaqI TCGA 4 cut(s) 51, 252, 430, 465
TasI AATT 3 cut(s) 103, 179, 591
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 141
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 451
TspRI CASTG 1 cut(s) 141
XceI RCATGY 1 cut(s) 134
XspI CTAG 2 cut(s) 362, 453
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.