Rh1DG209600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000400
Physical Location & Seq
Reverse (-)
40829288 .. 40832869
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG209600.1

Sequence Viewer

Length: 2907 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATCCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCTGGCTTTGGCTGTTGAAGCTCAAGAACGCTGCTCATGATGCTGATGACCTTCTGGATTTCGTTACGGCCAAAGGAAGAACTATGAGTCCTGGAAAAGGTAAGTGGACTTTAGCAATTCCAAAATCCATAGATGCTGACAAAGTCAGAAAGATGCTTTTGACATTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTACAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCGGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTAGTGAAACTGTTACTATCTTCTGAGTGTCACCAGGATGGTTATGCTATGTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGGATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAATCTCGACTTTGGCATTTGCTGTACAGCAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGAGGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAATTCCATTCATGATGGACTCCCCAACTTCGGCATATTATTTGAAGGGTTTGATGGAGGATGACTGCTGGGCTTTGTTCATGCAACGAGCTTTTGGACGAGGAGAAGAAGAGAAGTATCCCAACCTCTGCCTGATTGGAAAGAAGATTGCGAAGAAATGTGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACACCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCCAGACTTCGAAGTAATGACCGGTCTAAGACATCTCAATATAGCTGGATGTGATAGCTTGATTGCTCTACCTTCTGGTATTAAAAGGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCGCAAGAATCCCGGTAATCTTGGTGCACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACACTTGGAGAATGTAAGAAATGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATATTGAGTCGTTGGGATTATATTGGGGAGATTTTGAAGATGTGAACGATAGGAATAAATCATTGAAGAAACTAGCCAAGTCCCCAGCCAAAAGGTTTTTTTTAGAAGCAGGGGTACGGTGGCATGAACCTGGAAGCCTGGTACATGCTGAAAAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGATATACATGGTTATCCAGGAATCAGATTTCCAGATTGGACTCTCCCAAATCTAATTGCAGTTGATTTGTCCAATTGTAAGATCTGTGAATATCTTCCGGCCCTTGGGAATCTTCTTCTACTCAAGACTCTTTCTCTGCATGGAATGGAGAGCGTGAAGCGCATTGGCTTTGAGTTCTATGGTGATAGTACAGACATATACTTTTCAGCACTTGAAGAACTATTGCTCAGTGATTTTCCGAACTTGGAAGCCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAATTAACCATAAATAGGTGTCCCAAGTTGACACATATACCATATTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCAGTGGAGGTTTAACTTTGCTGACAGTGCTTGTAATAGAAAAACTTCCAGAGCTGCACTCTCTACCACAAGGGCTTTCTGCATCAGCTAGTCTGTCTTCTCTGGAGGTCTTGTCTTGCCCCAAGCTTTGTTCACTGCCTTTGGAGATGGGAAACCTCACTGCACTGAAATCTTTGACCATTCGTTGGTGTGATGAGTTGTCCTTTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAGTCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAATCTGACTTCTTTGTCATCGAGTTTGGAACAACTCGCTTTCCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCCCAGAGGGTGTGCAACACCTCTCAGCCCTTCGAAGTTTGTCTATCTTGAGCTGTCCTTGGTTTGATTCTCTGCCAGAAGGGTTACAAAATGTCAAGACGTTGCAATGTTTGGAAATTATTAGTTGCCCGAATCTAACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATCTTTGATTGTCCTAATTTGAAACTGTTGGCACAAAGTCTAAAACTTCTCACCAAACTCCAGCACCTCTCTATTCAAGAATGTCCCGAGCTTGAGGAAAGATGTAGACAAGGCAGTGGTGAGGATTGGCTGAAAATAGCTCATGTTCCACATAAGTACATTGGATCACTGGAGATGAGGCGGTCCAGTAGTGAAGCAAGTACATCAGGCAGCTCTTCTGTTGAAACATCTGTACAGCAAGCAACTGCTTCATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

968

Amino Acids

108.26

Weight (kDa)

6.02

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 86 2.8e-16 Rx N-terminal domain
NB-ARC PF00931 159 - 331 3.8e-36 NB-ARC domain
LRR_14 PF23598 349 - 505 4.4e-15 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 444 - 596 1.3e-25 R13L1/DRL21 LRRs
LRR_14 PF23598 640 - 763 2.5e-06 Leucine-rich repeat region
LRR_14 PF23598 664 - 803 5.9e-07 Leucine-rich repeat region
LRR_14 PF23598 817 - 910 2.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2386
AccB7I CCANNNNNTGG 2 cut(s) 2217, 2450
AccBSI CCGCTC 2 cut(s) 610, 2422
AccI GTMKAC 1 cut(s) 2752
AciI CCGC 6 cut(s) 610, 886, 1592, 2008, 2422, 2827
AclWI GGATC 1 cut(s) 2818
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 4 cut(s) 897, 1342, 1599, 1627
AcuI CTGAAG 4 cut(s) 828, 1268, 1442, 2017
AfaI GTAC 9 cut(s) 403, 767, 1551, 1578, 1831, 2438, 2804, 2848, 2880
AfiI CCNNNNNNNGG 7 cut(s) 281, 554, 925, 1745, 1950, 2217, 2450
AgeI ACCGGT 1 cut(s) 1205
AjnI CCWGG 7 cut(s) 274, 397, 522, 1564, 1572, 1657, 1892
AjuI GAANNNNNNNTTGG 2 cut(s) 2437, 2469
AloI GAACNNNNNNTCC 4 cut(s) 256, 288, 1120, 1152
Alw21I GWGCWC 2 cut(s) 1336, 1408
Alw26I GTCTC 3 cut(s) 425, 655, 1992
Alw44I GTGCAC 1 cut(s) 1332
AlwI GGATC 1 cut(s) 2818
AlwNI CAGNNNCTG 1 cut(s) 1291
Ama87I CYCGRG 1 cut(s) 2732
AoxI GGCC 3 cut(s) 252, 1609, 1740
ApaLI GTGCAC 1 cut(s) 1332
ApeKI GCWGC 4 cut(s) 215, 2005, 2086, 2856
ApoI RAATTY 4 cut(s) 897, 1342, 1599, 1627
AsiGI ACCGGT 1 cut(s) 1205
Asp700I GAANNNNTTC 2 cut(s) 1734, 2076
AspLEI GCGC 1 cut(s) 1803
AspS9I GGNCC 2 cut(s) 1741, 2829
AsuC2I CCSGG 1 cut(s) 1320
AsuHPI GGTGA 6 cut(s) 12, 512, 1835, 2419, 2689, 2777
AsuII TTCGAA 2 cut(s) 1194, 2491
AvaI CYCGRG 1 cut(s) 2732
AvaII GGWCC 1 cut(s) 2829
BaeGI GKGCMC 1 cut(s) 1336
BanII GRGCYC 1 cut(s) 1408
BarI GAAGNNNNNNTAC 6 cut(s) 1533, 1565, 2008, 2040, 2365, 2397
BbsI GAAGAC 1 cut(s) 2121
Bbv12I GWGCWC 2 cut(s) 1336, 1408
BbvI GCAGC 4 cut(s) 202, 1992, 2073, 2868
BccI CCATC 9 cut(s) 521, 854, 904, 943, 1601, 2029, 2173, 2312, 2654
BceAI ACGGC 2 cut(s) 167, 267
BciT130I CCWGG 7 cut(s) 276, 399, 524, 1566, 1574, 1659, 1894
BciVI GTATCC 1 cut(s) 1023
BcnI CCSGG 1 cut(s) 1320
BcoDI GTCTC 3 cut(s) 425, 655, 1992
BfaI CTAG 6 cut(s) 435, 1337, 1508, 1640, 2121, 2279
BfmI CTRYAG 1 cut(s) 2368
BfuI GTATCC 1 cut(s) 1023
BglII AGATCT 2 cut(s) 1722, 2636
BisI GCNGC 4 cut(s) 216, 2006, 2087, 2857
BlsI GCNGC 4 cut(s) 217, 2007, 2088, 2858
Bme1390I CCNGG 8 cut(s) 276, 399, 524, 1320, 1566, 1574, 1659, 1894
Bme18I GGWCC 1 cut(s) 2829
BmeT110I CYCGRG 1 cut(s) 2732
BmgT120I GGNCC 2 cut(s) 1741, 2829
BmrFI CCNGG 8 cut(s) 276, 399, 524, 1320, 1566, 1574, 1659, 1894
BmrI ACTGGG 2 cut(s) 53, 832
BmsI GCATC 8 cut(s) 62, 142, 214, 307, 327, 854, 2123, 2301
BmuI ACTGGG 2 cut(s) 53, 832
BoxI GACNNNNGTC 1 cut(s) 647
BpiI GAAGAC 1 cut(s) 2121
BplI GAGNNNNNCTC 4 cut(s) 422, 454, 2075, 2107
BpmI CTGGAG 6 cut(s) 89, 1422, 2156, 2291, 2690, 2837
Bpu14I TTCGAA 2 cut(s) 1194, 2491
BpuEI CTTGAG 6 cut(s) 191, 1748, 1951, 2438, 2527, 2759
BpuMI CCSGG 1 cut(s) 1320
BsaBI GATNNNNATC 1 cut(s) 2016
BsaJI CCNNGG 4 cut(s) 123, 1744, 2449, 2516
BsaWI WCCGGW 1 cut(s) 1205
BsaXI ACNNNNNCTCC 2 cut(s) 2129, 2159
Bsc4I CCNNNNNNNGG 7 cut(s) 281, 554, 925, 1745, 1950, 2217, 2450
Bse118I RCCGGY 2 cut(s) 1114, 1205
Bse1I ACTGG 5 cut(s) 59, 827, 1405, 2820, 2832
Bse3DI GCAATG 2 cut(s) 1139, 2570
Bse8I GATNNNNATC 1 cut(s) 2016
BseBI CCWGG 7 cut(s) 276, 399, 524, 1566, 1574, 1659, 1894
BseDI CCNNGG 4 cut(s) 123, 1744, 2449, 2516
BseGI GGATG 8 cut(s) 53, 532, 661, 799, 869, 961, 1169, 1238
BseJI GATNNNNATC 1 cut(s) 2016
BseLI CCNNNNNNNGG 7 cut(s) 281, 554, 925, 1745, 1950, 2217, 2450
BseMI GCAATG 2 cut(s) 1139, 2570
BseMII CTCAG 5 cut(s) 504, 602, 1882, 2496, 2647
BseNI ACTGG 5 cut(s) 59, 827, 1405, 2820, 2832
BseRI GAGGAG 3 cut(s) 173, 864, 1011
BseSI GKGCMC 1 cut(s) 1336
BseXI GCAGC 4 cut(s) 202, 1992, 2073, 2868
BseYI CCCAGC 2 cut(s) 963, 1519
BsgI GTGCAG 2 cut(s) 2072, 2178
BshFI GGCC 3 cut(s) 254, 1611, 1742
BshTI ACCGGT 1 cut(s) 1205
BsiHKAI GWGCWC 2 cut(s) 1336, 1408
BsiHKCI CYCGRG 1 cut(s) 2732
BsiSI CCGG 4 cut(s) 1115, 1206, 1320, 1739
BslFI GGGAC 5 cut(s) 101, 839, 1501, 1941, 2715
BslI CCNNNNNNNGG 7 cut(s) 281, 554, 925, 1745, 1950, 2217, 2450
BsmAI GTCTC 3 cut(s) 425, 655, 1992
BsmFI GGGAC 5 cut(s) 101, 839, 1501, 1941, 2715
BsnI GGCC 3 cut(s) 254, 1611, 1742
BsoBI CYCGRG 1 cut(s) 2732
Bsp119I TTCGAA 2 cut(s) 1194, 2491
Bsp1286I GDGCHC 2 cut(s) 1336, 1408
Bsp1407I TGTACA 2 cut(s) 765, 2878
Bsp143I GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
BspACI CCGC 6 cut(s) 610, 886, 1592, 2008, 2422, 2827
BspANI GGCC 3 cut(s) 254, 1611, 1742
BspCNI CTCAG 5 cut(s) 505, 601, 1881, 2495, 2646
BspHI TCATGA 4 cut(s) 220, 678, 906, 2903
BspMAI CTGCAG 1 cut(s) 2372
BspPI GGATC 1 cut(s) 2818
BspQI GCTCTTC 2 cut(s) 463, 2866
BspT104I TTCGAA 2 cut(s) 1194, 2491
BsrBI CCGCTC 2 cut(s) 610, 2422
BsrDI GCAATG 2 cut(s) 1139, 2570
BsrFI RCCGGY 2 cut(s) 1114, 1205
BsrGI TGTACA 2 cut(s) 765, 2878
BsrI ACTGG 5 cut(s) 59, 827, 1405, 2820, 2832
BssAI RCCGGY 2 cut(s) 1114, 1205
BssECI CCNNGG 4 cut(s) 123, 1744, 2449, 2516
BssMI GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
BssT1I CCWWGG 4 cut(s) 123, 1744, 2449, 2516
Bst2UI CCWGG 7 cut(s) 276, 399, 524, 1566, 1574, 1659, 1894
Bst4CI ACNGT 8 cut(s) 361, 406, 501, 1554, 2059, 2294, 2441, 2673
Bst6I CTCTTC 4 cut(s) 463, 999, 1995, 2866
BstAUI TGTACA 2 cut(s) 765, 2878
BstBI TTCGAA 2 cut(s) 1194, 2491
BstC8I GCNNGC 4 cut(s) 75, 1116, 2625, 2886
BstDEI CTNAG 8 cut(s) 513, 588, 833, 1175, 1211, 1868, 2482, 2633
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 8 cut(s) 53, 532, 661, 799, 869, 961, 1169, 1238
BstHHI GCGC 1 cut(s) 1803
BstKTI GATC 6 cut(s) 39, 678, 814, 1725, 2639, 2813
BstMAI GTCTC 3 cut(s) 425, 655, 1992
BstMBI GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
BstMWI GCNNNNNNNGC 7 cut(s) 202, 224, 593, 1403, 1589, 1800, 2059
BstNI CCWGG 7 cut(s) 276, 399, 524, 1566, 1574, 1659, 1894
BstNSI RCATGY 2 cut(s) 122, 1583
BstPAI GACNNNNGTC 1 cut(s) 647
BstSCI CCNGG 8 cut(s) 274, 397, 522, 1318, 1564, 1572, 1657, 1892
BstSFI CTRYAG 1 cut(s) 2368
BstSLI GKGCMC 1 cut(s) 1336
BstV1I GCAGC 4 cut(s) 202, 1992, 2073, 2868
BstV2I GAAGAC 1 cut(s) 2121
BstX2I RGATCY 2 cut(s) 1722, 2636
BstYI RGATCY 2 cut(s) 1722, 2636
BsuI GTATCC 1 cut(s) 1023
BsuRI GGCC 3 cut(s) 254, 1611, 1742
BtsCI GGATG 8 cut(s) 53, 532, 661, 799, 869, 961, 1169, 1238
BtsI GCAGTG 4 cut(s) 1289, 2165, 2190, 2767
BtsIMutI CAGTG 9 cut(s) 1289, 1876, 2041, 2064, 2165, 2190, 2195, 2767, 2813
Cac8I GCNNGC 4 cut(s) 75, 1116, 2625, 2886
CaiI CAGNNNCTG 1 cut(s) 1291
CciI TCATGA 4 cut(s) 220, 678, 906, 2903
CfoI GCGC 1 cut(s) 1803
Cfr10I RCCGGY 2 cut(s) 1114, 1205
Cfr13I GGNCC 2 cut(s) 1741, 2829
Csp6I GTAC 9 cut(s) 402, 766, 1550, 1577, 1830, 2437, 2803, 2847, 2879
CspAI ACCGGT 1 cut(s) 1205
CspCI CAANNNNNGTGG 2 cut(s) 2232, 2267
CviQI GTAC 9 cut(s) 402, 766, 1550, 1577, 1830, 2437, 2803, 2847, 2879
DdeI CTNAG 8 cut(s) 513, 588, 833, 1175, 1211, 1868, 2482, 2633
DpnI GATC 6 cut(s) 38, 677, 813, 1724, 2638, 2812
DpnII GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
DraI TTTAAA 1 cut(s) 1152
DrdI GACNNNNNNGTC 1 cut(s) 2386
DseDI GACNNNNNNGTC 1 cut(s) 2386
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 4 cut(s) 463, 999, 1995, 2866
EarI CTCTTC 4 cut(s) 463, 999, 1995, 2866
Ecl136II GAGCTC 1 cut(s) 1406
Eco130I CCWWGG 4 cut(s) 123, 1744, 2449, 2516
Eco24I GRGCYC 1 cut(s) 1408
Eco32I GATATC 2 cut(s) 82, 779
Eco47I GGWCC 1 cut(s) 2829
Eco53kI GAGCTC 1 cut(s) 1406
Eco57I CTGAAG 4 cut(s) 828, 1268, 1442, 2017
Eco88I CYCGRG 1 cut(s) 2732
EcoICRI GAGCTC 1 cut(s) 1406
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoRII CCWGG 7 cut(s) 274, 397, 522, 1564, 1572, 1657, 1892
EcoRV GATATC 2 cut(s) 82, 779
EcoT14I CCWWGG 4 cut(s) 123, 1744, 2449, 2516
EcoT22I ATGCAT 1 cut(s) 1921
EcoT38I GRGCYC 1 cut(s) 1408
ErhI CCWWGG 4 cut(s) 123, 1744, 2449, 2516
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 5 cut(s) 101, 839, 1501, 1941, 2715
FauI CCCGC 3 cut(s) 603, 893, 2001
FblI GTMKAC 1 cut(s) 2752
Fnu4HI GCNGC 4 cut(s) 216, 2006, 2087, 2857
FokI GGATG 8 cut(s) 40, 539, 668, 806, 876, 968, 1176, 1245
FriOI GRGCYC 1 cut(s) 1408
Fsp4HI GCNGC 4 cut(s) 216, 2006, 2087, 2857
FspBI CTAG 6 cut(s) 435, 1337, 1508, 1640, 2121, 2279
GlaI GCGC 1 cut(s) 1802
GluI GCNGC 4 cut(s) 216, 2006, 2087, 2857
GsaI CCCAGC 2 cut(s) 967, 1523
GsuI CTGGAG 6 cut(s) 89, 1422, 2156, 2291, 2690, 2837
HaeIII GGCC 3 cut(s) 254, 1611, 1742
HapII CCGG 4 cut(s) 1115, 1206, 1320, 1739
HhaI GCGC 1 cut(s) 1803
Hin6I GCGC 1 cut(s) 1801
HinP1I GCGC 1 cut(s) 1801
HincII GTYRAC 1 cut(s) 1965
HindII GTYRAC 1 cut(s) 1965
HindIII AAGCTT 3 cut(s) 71, 2156, 2621
HpaII CCGG 4 cut(s) 1115, 1206, 1320, 1739
HphI GGTGA 6 cut(s) 12, 512, 1835, 2419, 2689, 2777
Hpy166II GTNNAC 9 cut(s) 291, 364, 642, 1334, 1480, 1965, 2165, 2427, 2753
Hpy8I GTNNAC 9 cut(s) 291, 364, 642, 1334, 1480, 1965, 2165, 2427, 2753
HpyAV CCTTC 7 cut(s) 88, 245, 934, 1266, 2354, 2498, 2531
HpyCH4III ACNGT 8 cut(s) 361, 406, 501, 1554, 2059, 2294, 2441, 2673
HpyCH4IV ACGT 2 cut(s) 9, 2558
HpyF10VI GCNNNNNNNGC 7 cut(s) 202, 224, 593, 1403, 1589, 1800, 2059
HpyF3I CTNAG 8 cut(s) 513, 588, 833, 1175, 1211, 1868, 2482, 2633
HpySE526I ACGT 2 cut(s) 9, 2558
HspAI GCGC 1 cut(s) 1801
KroI GCCGGC 1 cut(s) 1114
KroNI GCCGGC 1 cut(s) 1116
Kzo9I GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
LguI GCTCTTC 2 cut(s) 463, 2866
LmnI GCTCC 3 cut(s) 160, 1403, 2002
Lsp1109I GCAGC 4 cut(s) 202, 1992, 2073, 2868
LweI GCATC 8 cut(s) 62, 142, 214, 307, 327, 854, 2123, 2301
MaeI CTAG 6 cut(s) 435, 1337, 1508, 1640, 2121, 2279
MaeII ACGT 2 cut(s) 9, 2558
MaeIII GTNAC 9 cut(s) 247, 501, 518, 700, 731, 1272, 2274, 2288, 2541
MalI GATC 6 cut(s) 38, 677, 813, 1724, 2638, 2812
MbiI CCGCTC 2 cut(s) 610, 2422
MboI GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
MfeI CAATTG 2 cut(s) 1714, 2325
MflI RGATCY 2 cut(s) 1722, 2636
MhlI GDGCHC 2 cut(s) 1336, 1408
MmeI TCCRAC 3 cut(s) 435, 643, 771
Mph1103I ATGCAT 1 cut(s) 1921
MroNI GCCGGC 1 cut(s) 1114
MroXI GAANNNNTTC 2 cut(s) 1734, 2076
MseI TTAA 5 cut(s) 384, 1151, 1266, 1940, 2045
MslI CAYNNNNRTG 4 cut(s) 525, 627, 2432, 2902
MspI CCGG 4 cut(s) 1115, 1206, 1320, 1739
MspR9I CCNGG 8 cut(s) 276, 399, 524, 1320, 1566, 1574, 1659, 1894
MunI CAATTG 2 cut(s) 1714, 2325
MvaI CCWGG 7 cut(s) 276, 399, 524, 1566, 1574, 1659, 1894
MwoI GCNNNNNNNGC 7 cut(s) 202, 224, 593, 1403, 1589, 1800, 2059
NaeI GCCGGC 1 cut(s) 1116
NciI CCSGG 1 cut(s) 1320
NdeII GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
NgoMIV GCCGGC 1 cut(s) 1114
NmuCI GTSAC 4 cut(s) 518, 731, 2274, 2288
NsiI ATGCAT 1 cut(s) 1921
NspI RCATGY 2 cut(s) 122, 1583
NspV TTCGAA 2 cut(s) 1194, 2491
PagI TCATGA 4 cut(s) 220, 678, 906, 2903
PciSI GCTCTTC 2 cut(s) 463, 2866
PdiI GCCGGC 1 cut(s) 1116
PdmI GAANNNNTTC 2 cut(s) 1734, 2076
PfeI GAWTC 7 cut(s) 1129, 1315, 1348, 1662, 1750, 2525, 2590
PflFI GACNNNGTC 1 cut(s) 326
PflMI CCANNNNNTGG 2 cut(s) 2217, 2450
PfoI TCCNGGA 2 cut(s) 274, 1657
PinAI ACCGGT 1 cut(s) 1205
PkrI GCNGC 4 cut(s) 217, 2007, 2088, 2858
PshAI GACNNNNGTC 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 1408
Psp6I CCWGG 7 cut(s) 274, 397, 522, 1564, 1572, 1657, 1892
PspFI CCCAGC 2 cut(s) 963, 1519
PspGI CCWGG 7 cut(s) 274, 397, 522, 1564, 1572, 1657, 1892
PspPI GGNCC 2 cut(s) 1741, 2829
PstI CTGCAG 1 cut(s) 2372
PstNI CAGNNNCTG 1 cut(s) 1291
PsuI RGATCY 2 cut(s) 1722, 2636
PsyI GACNNNGTC 1 cut(s) 326
RsaI GTAC 9 cut(s) 403, 767, 1551, 1578, 1831, 2438, 2804, 2848, 2880
RsaNI GTAC 9 cut(s) 402, 766, 1550, 1577, 1830, 2437, 2803, 2847, 2879
RseI CAYNNNNRTG 4 cut(s) 525, 627, 2432, 2902
SacI GAGCTC 1 cut(s) 1408
SapI GCTCTTC 2 cut(s) 463, 2866
SaqAI TTAA 5 cut(s) 384, 1151, 1266, 1940, 2045
SatI GCNGC 4 cut(s) 216, 2006, 2087, 2857
Sau3AI GATC 6 cut(s) 36, 675, 811, 1722, 2636, 2810
Sau96I GGNCC 2 cut(s) 1741, 2829
ScrFI CCNGG 8 cut(s) 276, 399, 524, 1320, 1566, 1574, 1659, 1894
SduI GDGCHC 2 cut(s) 1336, 1408
SfaNI GCATC 8 cut(s) 62, 142, 214, 307, 327, 854, 2123, 2301
SfcI CTRYAG 1 cut(s) 2368
SfuI TTCGAA 2 cut(s) 1194, 2491
SinI GGWCC 1 cut(s) 2829
SmiMI CAYNNNNRTG 4 cut(s) 525, 627, 2432, 2902
SmlI CTYRAG 6 cut(s) 206, 1763, 1930, 2417, 2506, 2738
SmoI CTYRAG 6 cut(s) 206, 1763, 1930, 2417, 2506, 2738
SsiI CCGC 6 cut(s) 610, 886, 1592, 2008, 2422, 2827
SspI AATATT 2 cut(s) 1369, 1438
SspMI CTAG 6 cut(s) 435, 1337, 1508, 1640, 2121, 2279
SstI GAGCTC 1 cut(s) 1408
StyD4I CCNGG 8 cut(s) 274, 397, 522, 1318, 1564, 1572, 1657, 1892
StyI CCWWGG 4 cut(s) 123, 1744, 2449, 2516
TaaI ACNGT 8 cut(s) 361, 406, 501, 1554, 2059, 2294, 2441, 2673
TaiI ACGT 2 cut(s) 12, 2561
TaqI TCGA 8 cut(s) 391, 748, 1083, 1194, 1898, 2309, 2393, 2491
TatI WGTACW 6 cut(s) 765, 1829, 2436, 2802, 2846, 2878
TfiI GAWTC 7 cut(s) 1129, 1315, 1348, 1662, 1750, 2525, 2590
Tru1I TTAA 5 cut(s) 384, 1151, 1266, 1940, 2045
Tru9I TTAA 5 cut(s) 384, 1151, 1266, 1940, 2045
TscAI CASTG 9 cut(s) 1296, 1876, 2041, 2064, 2172, 2197, 2202, 2767, 2820
TseFI GTSAC 4 cut(s) 518, 731, 2274, 2288
TseI GCWGC 4 cut(s) 215, 2005, 2086, 2856
Tsp45I GTSAC 4 cut(s) 518, 731, 2274, 2288
TspDTI ATGAA 9 cut(s) 107, 695, 895, 964, 1128, 1442, 1575, 1926, 2886
TspRI CASTG 9 cut(s) 1296, 1876, 2041, 2064, 2172, 2197, 2202, 2767, 2820
Tth111I GACNNNGTC 1 cut(s) 326
Van91I CCANNNNNTGG 2 cut(s) 2217, 2450
VneI GTGCAC 1 cut(s) 1332
VpaK11BI GGWCC 1 cut(s) 2829
XagI CCTNNNNNAGG 1 cut(s) 279
XapI RAATTY 4 cut(s) 897, 1342, 1599, 1627
XceI RCATGY 2 cut(s) 122, 1583
XmiI GTMKAC 1 cut(s) 2752
XmnI GAANNNNTTC 2 cut(s) 1734, 2076
XspI CTAG 6 cut(s) 435, 1337, 1508, 1640, 2121, 2279
Zsp2I ATGCAT 1 cut(s) 1921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.