RchiOBHm_Chr7g0232901

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000400
Physical Location & Seq
Reverse (-)
57087871 .. 57092473
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20875

Sequence Viewer

Length: 3777 bp
ATGCAGCAGACGTTGCAGCGGATTAGAGCGGCGATAGAAGATGCAGAGGAGCGGCAGAGGAAAAGTGATAGGGTGAAGGATTTGCTGATAAATCTCAAAGCAATCGCAGTCGATGCTGGTAATCTGCTTGATGAGGTTGCCACCCTAGTTCTGCGAAAGCAATTGATGAAAGAGGAATCTCACGGACTACGTGCTGCCACTAAGAAAGACTTATTTAGACTACGTTACCAATGCAACATGTCCAGGGTTATTCACACACCAGGTGCTCAGACCAGATATCTCATATTCAAGCTTTTTGAAGCACAAGAAGAGTTTGACAACGACATTATTCAAGGGATACTGCTTCATCTAGGAATTGGTGAGCTGCAGAGTACTAAAATACTGGCAGACTTTCAAAGTGCCCCACTTGCCAGTGGAAAGGGAAGTAAGCAGATGTATCTAACGAGGGTAGTTTATTATGAGCTTTATCTTTCACGTCCGAAGGACAGGATTGGTGTGATAATGAGAGAGATTGGAGAGCGTACAAAATATGAGGTAAGGAAAACTTGCTTAACTCTTGAGTCGATTTCTACTTACCGTAAGAAGTCACGCAGACTAAAGGAGCTTAACGCAAGATGGAATGATGTAGACAAGGAAATGTCTAGGTTCCAGTTCAAAAAAACTGAGTCTTATAGACGGTCAACTACCAGGGAGAGACGAGAAACTGGACCCTTTCCCAATGAGTCAATGGTCTATGGCAGAGAAGAGGATGTGAATAAGATTGTGCGGATGCTATTGTCTTCCAGTAGTGGTCCTGAAGTTGCAGTAATTCCCATTGTTGGAATTGGAGGGATGGGGAAAACAACACTTGCTCAGTTAGTCTACAATGATCCGAGGGTTACAAGCCACTTTCAATCTTTGATGTGGGTGTCTGTCAATGGTAAGTTTAACCCGGCAGAGATCATAAATCAAATGTTGAGTTATGTCAGAGAGGGCAGCCATGACCTGTTTCAGATTGGGTTGCTGCAATCTCAATTGAGAGAGTCCTTACTGGGAAGAAGATACTTAATAGTGCTTGATGATGTGTGGAATGTGGATGAAGATGAATGGGATAAAGTAATGAATCCATTGAAAGGCTCCGAGGGTGGAAGTAAAATTATATTAACCACCTGGAATGAGGCGGTTGCAGACATAACTAGGACATTTCCCTCTTTTCGTTTGGAACCACTAAGAAAAGAAGAGTGCTGGAAGTTGTTCAAGCACCGAGCCTTTGCAGATGGAACAGAGGATGATTTTCCGAGGCTACTGCAGATCGGTGAGAAAATAGTAGACAAGTGCCAAGGTGTCCCATTTGTTGCAAATATGCTTGGAATCATGCTGCGCTTTAAAAGAGAAGCAAACGATTGGTTACATGTGCAAGAAAGTGAACTATGGAGTATTGATGCAGGTGAAAACAGAATTCTATCCATCTACAGGCTGATTTACAATCATTTGCCATTGCATCTGATAGCTTGCTTTGCATATTGTTCAATATTCCCAAAGAATTATGAGATTAATAAGGAAAAGTTGATCCATCAATGGCTCGCACATGGCTTAATTCCTTATAATTCAGGGTCTTCATTTGAGCTGGAGGACATTGGCAATGAGTATTTTAAGGATTTACTAATGATGTCTTTCTTCCAAGAAGTAAGAAAACCTGATGACAGAGGTATGGCAGAATTTAAGATGCATGATCTTATTAATGATCTTGCAAAATCAGTAGCTGGTGAGGAATTCTTGACTCTGGGAGAGGAATCCTCGACTCTAGGACAGGATATTCTCTCAAAGACATGTCATGCATCAGTTGTTTGCAGTTCTAGCTCTATTTTGACCCCTGAAGCCTTGTGTGAAGCAAAGAGATTGCAAACCCTCAATTTCCTGTCACCAAGAGAGGATTATATGGAAGCCATTCCAACCGTACTAGCAACTTTCAAACATCTCAGAATGCTGAATTTCAGTGGATCTGGAATTAAGAGTCTCCACCAAGAGATTGGTGGGCTACTGTCCTTGCGGTATCTTGATCTATCAAATACTCTCCTAGAGACGATGCCAGCGACTATCTGTGGTCTGTGCAATTTGCAGACCCTGAATCTCTCAAGTTGTATTGAGCTCAAGGAGTTACCAAGTGGTACTACCAAGTTAATAAATCTGAGACATCTGAACATAGATGATTGTCCAAGACTTGCTGGCATGCCCCCATCCATGGGAATTTTACGACGACTTCAAACTTTGCCGGTATATATCGTCGGCCCCAACTTTGAAACTTCTATTTTTCAGCTCTCATCAATGAATCTACGAGGGAAGTTAAAACTCAAATGTCTGGAGGATGCTAAAATTCCGTCTGGGAACAACATGATTAAAGTATGGATGAAAACGAGAGAATTTTCTTCATTGGAACTGTTGTGGCAAAATGACGGGTGCAAGCTAGATCATAACAGATCTAGGCAAGCTGGCAGGCAAGTTGATGGTCAAACTGATCTTATTCTGGTGGATTCTTTGACTGTATCGCCCTCTATAAAAAAGTTGTCAATAAATGGTTATTCTGGAACCAAGTTCCCAGAGGAGATGAGTTGGCCTCGGAACTTGACTGAGCTAAATATAATTAATTGCAGAAGATGTGAAAGTCTTCCTCCACTCGGTCAACTTCCTGTCCTGAAGATCCTCAACATCCAAGGAATGGATTCTGTTGTGCGCATTGGTGTCGAATTCTCTGGTGAAGGTGACAGACCGTTTAGTTCTCTTAAAGAGCTATCCCTCAAAGACTTTCCTGAATTAAGAACTTGGCGTAGTATTGATTCCGGAGAAGTATTTACTTGCCTGGAAAAACTTATTATCACAAATTGTCCTTTTTTGACAACCATGCCATGGTTTCCACATCTCCGAGACTTGAAGCTGAGCAAGTGCATGCAGCTTGACTTAGTATGGTCAGTATCAAAGCTTACCACACTCTCTACTCTTGTTATTGACTCCTTTCCACAGTTGAGCTTTCTACCAAAAAAATTGGTGCAAAACAATTCACATCTGATATCATTAACTGTTACTTCCTGCCCCAATATTAGCTCACTACCTGAAAATCTGGGAAACCTCACTGCTCTGAAATCGCTGAAAATTGAATGGTGTCACGGGCTAGATACTTTGCCAAGTGGACTAAAGTACCTCACTTCACTGGAGAACTTGGAAATAGTTGATTGTCGTGGTTTAATCTGTTTGCCAGAGGAAGGCATGGAAGGCTTGTGCTCACTTCGGTCATTTTCAATTGAGAACTGTTTGAACTTAACCTCTTTGCCTATGGGGATGAAAAACCTCACATCCCTTGAGAACCTCATGCTTATGTGTTTAAATCTGGTTCATCTACCAGAGATTTTTCAATACCTCTTGGCACTTAGAAGCCTGACGATTAGAAGCTGTGAAGAGCTTACAAGTCTGCCGGTGGGACTGCAACATGTCCAGAATTTACAATTCTTGGAAATTCATCGCTGCCCGAAACTGATGGAATTGCCTGAGTGGGTGGAGCATCTTGTTTCACTTCGTTCCTTGAAAATCTTAGACTGCCGAGAAATAAAGTTCTTGCCAAAAGGTCTACAATGTCTTGGAGCGCTCCATCACCTGTCCATAATTGACTGTCCTGTCCTTGAGAAGCGCTGCGAGATGAAAACTGGTGAGGACTGGCAGAAGATATCTCATATTCCATATAAACATTTTGGATCATCAGCAGTGCAGCACAGGCAAGACATTGCATCCACCACACAGAATCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1258

Amino Acids

143.26

Weight (kDa)

7.47

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 1 - 59 5.1e-09 Rx N-terminal domain
NB-ARC PF00931 248 - 418 4.6e-37 NB-ARC domain
WHD_DRP PF23559 504 - 577 5.3e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 645 - 926 8.5e-24 Leucine-rich repeat region
LRR_8 PF13855 652 - 707 1.5e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 765 - 899 8.4e-21 R13L1/DRL21 LRRs
LRR_14 PF23598 959 - 1072 3e-07 Leucine-rich repeat region
LRR_14 PF23598 1080 - 1235 6.2e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1584
AarI CACCTGC 1 cut(s) 1415
AasI GACNNNNNNGTC 1 cut(s) 3644
Acc16I TGCGCA 1 cut(s) 2711
Acc36I ACCTGC 1 cut(s) 1415
AccB7I CCANNNNNTGG 2 cut(s) 2695, 2883
AccBSI CCGCTC 2 cut(s) 29, 52
AccI GTMKAC 4 cut(s) 627, 861, 1308, 3598
AccIII TCCGGA 1 cut(s) 2816
AciI CCGC 6 cut(s) 19, 29, 52, 766, 1160, 2029
AclWI GGATC 5 cut(s) 863, 1543, 1987, 2671, 3730
AcuI CTGAAG 3 cut(s) 816, 1875, 2693
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 5 cut(s) 373, 523, 1938, 2149, 3173
AfeI AGCGCT 2 cut(s) 3615, 3659
AfiI CCNNNNNNNGG 9 cut(s) 818, 1112, 1452, 2221, 2222, 2654, 2695, 2883, 3236
AflIII ACRYGT 4 cut(s) 237, 1390, 1808, 3460
AjiI CACGTC 1 cut(s) 476
AjnI CCWGG 5 cut(s) 242, 259, 686, 1148, 2835
Alw21I GWGCWC 3 cut(s) 268, 2130, 3257
Alw26I GTCTC 5 cut(s) 688, 2000, 2054, 2164, 2895
AlwI GGATC 5 cut(s) 863, 1543, 1987, 2671, 3730
AlwNI CAGNNNCTG 2 cut(s) 1742, 2104
Aor13HI TCCGGA 1 cut(s) 2816
Aor51HI AGCGCT 2 cut(s) 3615, 3659
AoxI GGCC 2 cut(s) 2266, 2591
AseI ATTAAT 3 cut(s) 1533, 1719, 2622
Asp700I GAANNNNTTC 4 cut(s) 1232, 1926, 2643, 2698
AspLEI GCGC 4 cut(s) 1362, 2712, 3616, 3660
AspS9I GGNCC 3 cut(s) 707, 791, 2267
AsuC2I CCSGG 1 cut(s) 932
AvaII GGWCC 2 cut(s) 707, 791
BaeGI GKGCMC 1 cut(s) 403
BaeI ACNNNNGTAYC 2 cut(s) 3155, 3188
BanII GRGCYC 1 cut(s) 2130
BbsI GAAGAC 3 cut(s) 771, 1587, 2636
Bbv12I GWGCWC 3 cut(s) 268, 2130, 3257
BccI CCATC 9 cut(s) 609, 826, 1250, 1454, 1560, 2224, 2477, 3502, 3627
BcgI CGANNNNNNTGC 4 cut(s) 1267, 1301, 2701, 2735
BciT130I CCWGG 5 cut(s) 244, 261, 688, 1150, 2837
BciVI GTATCC 1 cut(s) 330
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 5 cut(s) 688, 2000, 2054, 2164, 2895
BfmI CTRYAG 3 cut(s) 365, 1286, 1450
BfoI RGCGCY 2 cut(s) 3617, 3661
BfuAI ACCTGC 1 cut(s) 1415
BfuI GTATCC 1 cut(s) 330
BglII AGATCT 1 cut(s) 2456
BlpI GCTNAGC 1 cut(s) 2912
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 6 cut(s) 244, 261, 688, 932, 1150, 2837
Bme18I GGWCC 2 cut(s) 707, 791
BmgBI CACGTC 1 cut(s) 476
BmgT120I GGNCC 3 cut(s) 707, 791, 2267
BmiI GGNNCC 6 cut(s) 647, 709, 1117, 1203, 2269, 2566
BmrFI CCNGG 6 cut(s) 244, 261, 688, 932, 1150, 2837
BmrI ACTGGG 1 cut(s) 1040
BmuI ACTGGG 1 cut(s) 1040
BpiI GAAGAC 3 cut(s) 771, 1587, 2636
BplI GAGNNNNNCTC 4 cut(s) 1760, 1792, 2578, 2610
BpmI CTGGAG 3 cut(s) 1628, 2360, 3206
Bpu1102I GCTNAGC 1 cut(s) 2912
BpuEI CTTGAG 5 cut(s) 578, 2098, 2114, 3353, 3671
BpuMI CCSGG 1 cut(s) 932
BsaAI YACGTR 1 cut(s) 191
BsaWI WCCGGW 1 cut(s) 2816
Bsc4I CCNNNNNNNGG 9 cut(s) 818, 1112, 1452, 2221, 2222, 2654, 2695, 2883, 3236
Bse118I RCCGGY 2 cut(s) 2251, 3445
Bse1I ACTGG 9 cut(s) 387, 411, 649, 709, 783, 1035, 3189, 3679, 3689
Bse3DI GCAATG 3 cut(s) 1475, 1627, 3750
BseAI TCCGGA 1 cut(s) 2816
BseBI CCWGG 5 cut(s) 244, 261, 688, 1150, 2837
BseLI CCNNNNNNNGG 9 cut(s) 818, 1112, 1452, 2221, 2222, 2654, 2695, 2883, 3236
BseMI GCAATG 3 cut(s) 1475, 1627, 3750
BseMII CTCAG 8 cut(s) 281, 654, 866, 1972, 2159, 2598, 2903, 3510
BseNI ACTGG 9 cut(s) 387, 411, 649, 709, 783, 1035, 3189, 3679, 3689
BseRI GAGGAG 2 cut(s) 62, 2594
BseSI GKGCMC 1 cut(s) 403
BsgI GTGCAG 1 cut(s) 3755
BshFI GGCC 2 cut(s) 2268, 2593
BsiHKAI GWGCWC 3 cut(s) 268, 2130, 3257
BsiSI CCGG 4 cut(s) 932, 2252, 2817, 3446
BslFI GGGAC 2 cut(s) 1310, 3465
BslI CCNNNNNNNGG 9 cut(s) 818, 1112, 1452, 2221, 2222, 2654, 2695, 2883, 3236
BsmAI GTCTC 5 cut(s) 688, 2000, 2054, 2164, 2895
BsmBI CGTCTC 2 cut(s) 688, 2054
BsmFI GGGAC 2 cut(s) 1310, 3465
BsmI GAATGC 1 cut(s) 1968
BsnI GGCC 2 cut(s) 2268, 2593
Bsp1286I GDGCHC 4 cut(s) 268, 403, 2130, 3257
Bsp13I TCCGGA 1 cut(s) 2816
Bsp1720I GCTNAGC 1 cut(s) 2912
Bsp19I CCATGG 2 cut(s) 2220, 2882
BspACI CCGC 6 cut(s) 19, 29, 52, 766, 1160, 2029
BspANI GGCC 2 cut(s) 2268, 2593
BspCNI CTCAG 8 cut(s) 280, 655, 865, 1971, 2160, 2599, 2904, 3511
BspEI TCCGGA 1 cut(s) 2816
BspLI GGNNCC 6 cut(s) 647, 709, 1117, 1203, 2269, 2566
BspMAI CTGCAG 2 cut(s) 369, 1290
BspMI ACCTGC 1 cut(s) 1415
BspPI GGATC 5 cut(s) 863, 1543, 1987, 2671, 3730
BspQI GCTCTTC 1 cut(s) 3423
BsrBI CCGCTC 2 cut(s) 29, 52
BsrDI GCAATG 3 cut(s) 1475, 1627, 3750
BsrFI RCCGGY 2 cut(s) 2251, 3445
BsrI ACTGG 9 cut(s) 387, 411, 649, 709, 783, 1035, 3189, 3679, 3689
BssAI RCCGGY 2 cut(s) 2251, 3445
BssT1I CCWWGG 4 cut(s) 1318, 2220, 2689, 2882
Bst2UI CCWGG 5 cut(s) 244, 261, 688, 1150, 2837
Bst6I CTCTTC 4 cut(s) 303, 738, 1212, 3423
BstAPI GCANNNNNTGC 2 cut(s) 13, 113
BstBAI YACGTR 1 cut(s) 191
BstDSI CCRYGG 2 cut(s) 2220, 2882
BstH2I RGCGCY 2 cut(s) 3617, 3661
BstHHI GCGC 4 cut(s) 1362, 2712, 3616, 3660
BstMAI GTCTC 5 cut(s) 688, 2000, 2054, 2164, 2895
BstMWI GCNNNNNNNGC 7 cut(s) 13, 113, 407, 1496, 1836, 3246, 3742
BstNI CCWGG 5 cut(s) 244, 261, 688, 1150, 2837
BstNSI RCATGY 6 cut(s) 241, 1394, 1812, 2212, 2926, 3464
BstSCI CCNGG 6 cut(s) 242, 259, 686, 930, 1148, 2835
BstSFI CTRYAG 3 cut(s) 365, 1286, 1450
BstSLI GKGCMC 1 cut(s) 403
BstV2I GAAGAC 3 cut(s) 771, 1587, 2636
BstX2I RGATCY 3 cut(s) 1979, 2456, 2676
BstXI CCANNNNNNTGG 1 cut(s) 2009
BstYI RGATCY 3 cut(s) 1979, 2456, 2676
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 2 cut(s) 2268, 2593
BtgI CCRYGG 2 cut(s) 2220, 2882
BtgZI GCGATG 1 cut(s) 3476
BtrI CACGTC 1 cut(s) 476
BtsI GCAGTG 2 cut(s) 3105, 3738
BtsIMutI CAGTG 5 cut(s) 418, 1981, 3105, 3182, 3738
BveI ACCTGC 1 cut(s) 1415
CaiI CAGNNNCTG 2 cut(s) 1742, 2104
CfoI GCGC 4 cut(s) 1362, 2712, 3616, 3660
Cfr10I RCCGGY 2 cut(s) 2251, 3445
Cfr13I GGNCC 3 cut(s) 707, 791, 2267
CsiI ACCWGGT 1 cut(s) 259
Csp6I GTAC 5 cut(s) 372, 522, 1937, 2148, 3172
CviQI GTAC 5 cut(s) 372, 522, 1937, 2148, 3172
DraI TTTAAA 2 cut(s) 1366, 3357
DraIII CACNNNGTG 1 cut(s) 263
DrdI GACNNNNNNGTC 1 cut(s) 3644
DseDI GACNNNNNNGTC 1 cut(s) 3644
Eam1104I CTCTTC 4 cut(s) 303, 738, 1212, 3423
EarI CTCTTC 4 cut(s) 303, 738, 1212, 3423
Ecl136II GAGCTC 1 cut(s) 2128
Eco130I CCWWGG 4 cut(s) 1318, 2220, 2689, 2882
Eco24I GRGCYC 1 cut(s) 2130
Eco32I GATATC 3 cut(s) 278, 3045, 3696
Eco47I GGWCC 2 cut(s) 707, 791
Eco47III AGCGCT 2 cut(s) 3615, 3659
Eco53kI GAGCTC 1 cut(s) 2128
Eco57I CTGAAG 3 cut(s) 816, 1875, 2693
EcoICRI GAGCTC 1 cut(s) 2128
EcoRI GAATTC 3 cut(s) 1437, 1751, 2723
EcoRII CCWGG 5 cut(s) 242, 259, 686, 1148, 2835
EcoRV GATATC 3 cut(s) 278, 3045, 3696
EcoT14I CCWWGG 4 cut(s) 1318, 2220, 2689, 2882
EcoT22I ATGCAT 2 cut(s) 1710, 1819
EcoT38I GRGCYC 1 cut(s) 2130
ErhI CCWWGG 4 cut(s) 1318, 2220, 2689, 2882
Esp3I CGTCTC 2 cut(s) 688, 2054
FalI AAGNNNNNCTT 4 cut(s) 194, 226, 529, 561
FaqI GGGAC 2 cut(s) 1310, 3465
FblI GTMKAC 4 cut(s) 627, 861, 1308, 3598
FriOI GRGCYC 1 cut(s) 2130
FspAI RTGCGCAY 1 cut(s) 2711
FspI TGCGCA 1 cut(s) 2711
GlaI GCGC 4 cut(s) 1361, 2711, 3615, 3659
GsuI CTGGAG 3 cut(s) 1628, 2360, 3206
HaeII RGCGCY 2 cut(s) 3617, 3661
HaeIII GGCC 2 cut(s) 2268, 2593
HapII CCGG 4 cut(s) 932, 2252, 2817, 3446
HhaI GCGC 4 cut(s) 1362, 2712, 3616, 3660
Hin6I GCGC 4 cut(s) 1360, 2710, 3614, 3658
HinP1I GCGC 4 cut(s) 1360, 2710, 3614, 3658
HincII GTYRAC 2 cut(s) 681, 2660
HindII GTYRAC 2 cut(s) 681, 2660
HindIII AAGCTT 2 cut(s) 290, 2954
HpaII CCGG 4 cut(s) 932, 2252, 2817, 3446
Hpy166II GTNNAC 8 cut(s) 628, 681, 862, 1309, 1406, 2660, 3164, 3599
Hpy8I GTNNAC 8 cut(s) 628, 681, 862, 1309, 1406, 2660, 3164, 3599
Hpy99I CGWCG 2 cut(s) 2238, 2267
HpyAV CCTTC 5 cut(s) 70, 475, 2729, 3230, 3239
HpyCH4IV ACGT 4 cut(s) 11, 190, 223, 475
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 113, 407, 1496, 1836, 3246, 3742
HpySE526I ACGT 4 cut(s) 11, 190, 223, 475
HspAI GCGC 4 cut(s) 1360, 2710, 3614, 3658
Kpn2I TCCGGA 1 cut(s) 2816
LguI GCTCTTC 1 cut(s) 3423
LmnI GCTCC 6 cut(s) 49, 601, 1121, 3529, 3611, 3621
MabI ACCWGGT 1 cut(s) 259
MaeII ACGT 4 cut(s) 11, 190, 223, 475
MaeIII GTNAC 9 cut(s) 224, 585, 877, 1386, 1899, 2136, 2738, 3055, 3137
MbiI CCGCTC 2 cut(s) 29, 52
MfeI CAATTG 3 cut(s) 161, 1013, 3273
MflI RGATCY 3 cut(s) 1979, 2456, 2676
MhlI GDGCHC 4 cut(s) 268, 403, 2130, 3257
MlyI GAGTC 8 cut(s) 569, 674, 731, 1031, 1753, 1774, 2002, 2978
MmeI TCCRAC 2 cut(s) 799, 1955
Mph1103I ATGCAT 2 cut(s) 1710, 1819
MroI TCCGGA 1 cut(s) 2816
MroXI GAANNNNTTC 4 cut(s) 1232, 1926, 2643, 2698
MslI CAYNNNNRTG 1 cut(s) 3347
MspA1I CMGCKG 1 cut(s) 19
MspI CCGG 4 cut(s) 932, 2252, 2817, 3446
MspR9I CCNGG 6 cut(s) 244, 261, 688, 932, 1150, 2837
MunI CAATTG 3 cut(s) 161, 1013, 3273
Mva1269I GAATGC 1 cut(s) 1968
MvaI CCWGG 5 cut(s) 244, 261, 688, 1150, 2837
MwoI GCNNNNNNNGC 7 cut(s) 13, 113, 407, 1496, 1836, 3246, 3742
NciI CCSGG 1 cut(s) 932
NcoI CCATGG 2 cut(s) 2220, 2882
NlaIV GGNNCC 6 cut(s) 647, 709, 1117, 1203, 2269, 2566
NmeAIII GCCGAG 1 cut(s) 3596
NmuCI GTSAC 4 cut(s) 585, 1899, 2738, 3137
NsbI TGCGCA 1 cut(s) 2711
NsiI ATGCAT 2 cut(s) 1710, 1819
NspI RCATGY 6 cut(s) 241, 1394, 1812, 2212, 2926, 3464
PaeI GCATGC 2 cut(s) 2212, 2926
PaqCI CACCTGC 1 cut(s) 1415
PciI ACATGT 4 cut(s) 237, 1390, 1808, 3460
PciSI GCTCTTC 1 cut(s) 3423
PcsI WCGNNNNNNNCGW 1 cut(s) 2069
PctI GAATGC 1 cut(s) 1968
PdmI GAANNNNTTC 4 cut(s) 1232, 1926, 2643, 2698
PflMI CCANNNNNTGG 2 cut(s) 2695, 2883
PleI GAGTC 8 cut(s) 568, 673, 730, 1030, 1753, 1774, 2001, 2978
PpsI GAGTC 8 cut(s) 568, 673, 730, 1030, 1753, 1774, 2001, 2978
Ppu21I YACGTR 1 cut(s) 191
PscI ACATGT 4 cut(s) 237, 1390, 1808, 3460
PshBI ATTAAT 3 cut(s) 1533, 1719, 2622
PsiI TTATAA 1 cut(s) 1584
Psp124BI GAGCTC 1 cut(s) 2130
Psp6I CCWGG 5 cut(s) 242, 259, 686, 1148, 2835
PspGI CCWGG 5 cut(s) 242, 259, 686, 1148, 2835
PspN4I GGNNCC 6 cut(s) 647, 709, 1117, 1203, 2269, 2566
PspPI GGNCC 3 cut(s) 707, 791, 2267
PstI CTGCAG 2 cut(s) 369, 1290
PstNI CAGNNNCTG 2 cut(s) 1742, 2104
PsuI RGATCY 3 cut(s) 1979, 2456, 2676
RsaI GTAC 5 cut(s) 373, 523, 1938, 2149, 3173
RsaNI GTAC 5 cut(s) 372, 522, 1937, 2148, 3172
RseI CAYNNNNRTG 1 cut(s) 3347
SacI GAGCTC 1 cut(s) 2130
SapI GCTCTTC 1 cut(s) 3423
Sau96I GGNCC 3 cut(s) 707, 791, 2267
ScaI AGTACT 1 cut(s) 373
SchI GAGTC 8 cut(s) 569, 674, 731, 1031, 1753, 1774, 2002, 2978
ScrFI CCNGG 6 cut(s) 244, 261, 688, 932, 1150, 2837
SduI GDGCHC 4 cut(s) 268, 403, 2130, 3257
SexAI ACCWGGT 1 cut(s) 259
SfcI CTRYAG 3 cut(s) 365, 1286, 1450
SinI GGWCC 2 cut(s) 707, 791
SmiMI CAYNNNNRTG 1 cut(s) 3347
SmlI CTYRAG 5 cut(s) 557, 2113, 2129, 3332, 3650
SmoI CTYRAG 5 cut(s) 557, 2113, 2129, 3332, 3650
SphI GCATGC 2 cut(s) 2212, 2926
SsiI CCGC 6 cut(s) 19, 29, 52, 766, 1160, 2029
SspI AATATT 2 cut(s) 1512, 3073
SstI GAGCTC 1 cut(s) 2130
StyD4I CCNGG 6 cut(s) 242, 259, 686, 930, 1148, 2835
StyI CCWWGG 4 cut(s) 1318, 2220, 2689, 2882
TaiI ACGT 4 cut(s) 14, 193, 226, 478
TaqI TCGA 4 cut(s) 111, 563, 1778, 2721
TaqII GACCGA 2 cut(s) 2645, 3252
TatI WGTACW 1 cut(s) 371
TauI GCSGC 2 cut(s) 32, 55
TscAI CASTG 5 cut(s) 418, 1981, 3112, 3189, 3738
TseFI GTSAC 4 cut(s) 585, 1899, 2738, 3137
Tsp45I GTSAC 4 cut(s) 585, 1899, 2738, 3137
TspGWI ACGGA 2 cut(s) 198, 2346
TspRI CASTG 5 cut(s) 418, 1981, 3112, 3189, 3738
Van91I CCANNNNNTGG 2 cut(s) 2695, 2883
VpaK11BI GGWCC 2 cut(s) 707, 791
VspI ATTAAT 3 cut(s) 1533, 1719, 2622
XceI RCATGY 6 cut(s) 241, 1394, 1812, 2212, 2926, 3464
XcmI CCANNNNNNNNNTGG 3 cut(s) 724, 2006, 2009
XmiI GTMKAC 4 cut(s) 627, 861, 1308, 3598
XmnI GAANNNNTTC 4 cut(s) 1232, 1926, 2643, 2698
ZrmI AGTACT 1 cut(s) 373
Zsp2I ATGCAT 2 cut(s) 1710, 1819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.