Rroxscaffold_4G00306950

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000400
Physical Location & Seq
Forward (+)
27949264 .. 27952809
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306950.1

Sequence Viewer

Length: 2682 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCTGATATCTGGGACATGAAGGATAACCTCCAGAGCCTGCAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTTGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGACTTCTTTGCTGCTAAAGACATCATTATGGGTCCTGGAAAATTATGTAAGTGGACCTATCCAATTCCAAAATACATTGATGCTGATAAAGTCAGAAAGATGCTTTTGGCTTTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTTCAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTATGGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGGAGTTAGTGAAACTGTTACTATCTTCTGAGTGTCACCAGGATGGATATGCTATGTGTATTCCGATAATTGGTATCGGAGGAATTGGCAAGACGACTCTTGCTCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATGTTAGGCTGTGGACTTTTGTCTCGGATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAGATTGCAAGTTCTCGGAGAGTGACTTACTTCAAGCTCGGCTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGAGGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAACAAAAAAGTCCCATTCATGATGGACTCCCCAACTTCGCTATATTATTTGAAGGGTTTGATGGAGGATGGCTGCTGGGCTTTGTTCAAGCAACGAGCTTTTGGACGAGGAGAAGAAGAGAAGTATCCCAACCTCTGCCTGATTGGAAAGAAGATTGTGAAGAAATGTGGTGGTGTACCATTAGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTGTGGGAAGTGGAAGGATGCAAACAAAGAATTTTACCCTCGCTTATGTTGAGTTATCTTCATCTGTCATCACATCTTCGACAATGCTTTGCATTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAGGTAGCAAGCGACCGGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGAAGGTGGCAGCACAGTTGGATACAAAATGAATGATGTCATTCATGACCTTGCACGATATGTTTCTGGAAATGGATCCGTTATGCATGAACAAGGCCTTCCACCAAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGAACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAGTTGTATATACTTGCGTGTGCTGGACTTGAACAACTGTGTTGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACACCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCGTTGCAGACTTTAAACCTCTCTGGATGTCATCATCTTAGAGTCTTGCCAGACTTCGAAGTAATGACCGGCCTAAGACATCTCAATATAGCTGGATGTGATAGCTTGTGGTATATACCTCCTGGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCACAAGAGGGTCGGTGATCTTGGTGTACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACACTTGGAGAATGTGAGAAATGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATCTTGAGTGGTTGGGATTATATTGGGGAGATTTTGAACATGTGAACGACAGGAAAAAATCATTGAAGAAACTAGCCAAGTCCCCGGCCAAAAGGTTTTTTTTGGAAGCAGGGGTACGGTGGCATGAACCTGATGCTGAAGAGGCTGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAATCAGATTTCCAGATTGGACTCTCCCAAATGTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCTTCTACTAAAGACTCTTTCTCTGCACGGAATGGAGAGCGTGACGCGCATTGGCTTTGAGTTCTATGGTGATAGTACAGACACATGCTTTTCATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAACTTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATAAATAGGTGTCAAGTTGACACATATACCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

893

Amino Acids

101.8

Weight (kDa)

6.26

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 86 2.9e-18 Rx N-terminal domain
NB-ARC PF00931 161 - 332 5.2e-36 NB-ARC domain
WHD_DRP PF23559 418 - 489 6.3e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 553 - 877 2.8e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 670 - 818 3.1e-26 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1557
AccB7I CCANNNNNTGG 1 cut(s) 2411
AccBSI CCGCTC 2 cut(s) 613, 1542
AccII CGCG 1 cut(s) 2467
AciI CCGC 4 cut(s) 613, 889, 1540, 2672
AclWI GGATC 2 cut(s) 1482, 1495
AcoI YGGCCR 1 cut(s) 2199
AcsI RAATTY 6 cut(s) 1179, 1271, 1533, 2020, 2265, 2293
AcuI CTGAAG 4 cut(s) 831, 1946, 2120, 2271
AfaI GTAC 4 cut(s) 1068, 2013, 2229, 2497
AfiI CCNNNNNNNGG 3 cut(s) 557, 2411, 2616
AflIII ACRYGT 1 cut(s) 2152
AjnI CCWGG 6 cut(s) 274, 400, 525, 1936, 2323, 2558
AloI GAACNNNNNNTCC 2 cut(s) 1798, 1830
Alw21I GWGCWC 1 cut(s) 2086
Alw26I GTCTC 3 cut(s) 428, 658, 2656
AlwI GGATC 2 cut(s) 1482, 1495
AlwNI CAGNNNCTG 2 cut(s) 112, 1969
AoxI GGCC 4 cut(s) 1507, 1885, 2199, 2275
ApeKI GCWGC 7 cut(s) 215, 251, 766, 963, 1076, 1422, 2669
ApoI RAATTY 6 cut(s) 1179, 1271, 1533, 2020, 2265, 2293
Asp700I GAANNNNTTC 1 cut(s) 2400
AspLEI GCGC 2 cut(s) 1104, 2469
AspS9I GGNCC 2 cut(s) 272, 294
AsuC2I CCSGG 1 cut(s) 2198
AsuHPI GGTGA 5 cut(s) 12, 515, 1367, 2012, 2501
AsuII TTCGAA 1 cut(s) 1872
AvaII GGWCC 2 cut(s) 272, 294
BamHI GGATCC 1 cut(s) 1487
BanII GRGCYC 1 cut(s) 2086
BarI GAAGNNNNNNTAC 4 cut(s) 1392, 1424, 2211, 2243
BbsI GAAGAC 1 cut(s) 1353
Bbv12I GWGCWC 1 cut(s) 2086
BbvI GCAGC 7 cut(s) 227, 238, 753, 950, 1088, 1434, 2656
BccI CCATC 7 cut(s) 524, 857, 907, 946, 953, 1297, 2267
BceAI ACGGC 3 cut(s) 167, 1136, 1708
BciT130I CCWGG 6 cut(s) 276, 402, 527, 1938, 2325, 2560
BciVI GTATCC 2 cut(s) 1026, 1427
BcnI CCSGG 1 cut(s) 2198
BcoDI GTCTC 3 cut(s) 428, 658, 2656
BfaI CTAG 5 cut(s) 438, 1322, 2015, 2186, 2306
BfmI CTRYAG 1 cut(s) 2259
BfuI GTATCC 2 cut(s) 1026, 1427
BisI GCNGC 8 cut(s) 216, 252, 767, 964, 1077, 1423, 1540, 2670
BlsI GCNGC 8 cut(s) 217, 253, 768, 965, 1078, 1424, 1541, 2671
Bme1390I CCNGG 7 cut(s) 276, 402, 527, 1938, 2198, 2325, 2560
Bme18I GGWCC 2 cut(s) 272, 294
BmgT120I GGNCC 2 cut(s) 272, 294
BmiI GGNNCC 2 cut(s) 273, 1489
BmrFI CCNGG 7 cut(s) 276, 402, 527, 1938, 2198, 2325, 2560
BmrI ACTGGG 2 cut(s) 53, 835
BmsI GCATC 8 cut(s) 62, 142, 214, 310, 330, 857, 1157, 2236
BmuI ACTGGG 2 cut(s) 53, 835
BoxI GACNNNNGTC 1 cut(s) 650
BpiI GAAGAC 1 cut(s) 1353
BplI GAGNNNNNCTC 2 cut(s) 425, 457
BpmI CTGGAG 2 cut(s) 89, 2100
Bpu14I TTCGAA 1 cut(s) 1872
BpuEI CTTGAG 3 cut(s) 191, 2138, 2617
BpuMI CCSGG 1 cut(s) 2198
BsaAI YACGTR 1 cut(s) 1412
BsaJI CCNNGG 3 cut(s) 123, 2196, 2410
BsaWI WCCGGW 1 cut(s) 1342
Bsc4I CCNNNNNNNGG 3 cut(s) 557, 2411, 2616
Bse118I RCCGGY 2 cut(s) 1792, 1883
Bse1I ACTGG 3 cut(s) 59, 830, 2083
Bse3DI GCAATG 1 cut(s) 1130
BseBI CCWGG 6 cut(s) 276, 402, 527, 1938, 2325, 2560
BseDI CCNNGG 3 cut(s) 123, 2196, 2410
BseLI CCNNNNNNNGG 3 cut(s) 557, 2411, 2616
BseMI GCAATG 1 cut(s) 1130
BseMII CTCAG 3 cut(s) 507, 605, 2548
BseNI ACTGG 3 cut(s) 59, 830, 2083
BseRI GAGGAG 4 cut(s) 173, 503, 867, 1014
BseXI GCAGC 7 cut(s) 227, 238, 753, 950, 1088, 1434, 2656
BseYI CCCAGC 1 cut(s) 966
BsgI GTGCAG 2 cut(s) 752, 2429
Bsh1236I CGCG 1 cut(s) 2467
Bsh1285I CGRYCG 1 cut(s) 1343
BshFI GGCC 4 cut(s) 1509, 1887, 2201, 2277
BsiEI CGRYCG 1 cut(s) 1343
BsiHKAI GWGCWC 1 cut(s) 2086
BsiSI CCGG 5 cut(s) 187, 1343, 1793, 1884, 2198
BslFI GGGAC 4 cut(s) 101, 842, 887, 2179
BslI CCNNNNNNNGG 3 cut(s) 557, 2411, 2616
BsmAI GTCTC 3 cut(s) 428, 658, 2656
BsmFI GGGAC 4 cut(s) 101, 842, 887, 2179
BsmI GAATGC 1 cut(s) 1241
BsnI GGCC 4 cut(s) 1509, 1887, 2201, 2277
Bsp119I TTCGAA 1 cut(s) 1872
Bsp1286I GDGCHC 1 cut(s) 2086
Bsp143I GATC 5 cut(s) 36, 678, 814, 1487, 2002
BspACI CCGC 4 cut(s) 613, 889, 1540, 2672
BspANI GGCC 4 cut(s) 1509, 1887, 2201, 2277
BspCNI CTCAG 3 cut(s) 508, 604, 2547
BspFNI CGCG 1 cut(s) 2467
BspHI TCATGA 4 cut(s) 220, 681, 909, 1456
BspLI GGNNCC 2 cut(s) 273, 1489
BspPI GGATC 2 cut(s) 1482, 1495
BspQI GCTCTTC 1 cut(s) 466
BspT104I TTCGAA 1 cut(s) 1872
BsrBI CCGCTC 2 cut(s) 613, 1542
BsrDI GCAATG 1 cut(s) 1130
BsrFI RCCGGY 2 cut(s) 1792, 1883
BsrI ACTGG 3 cut(s) 59, 830, 2083
BssAI RCCGGY 2 cut(s) 1792, 1883
BssECI CCNNGG 3 cut(s) 123, 2196, 2410
BssMI GATC 5 cut(s) 36, 678, 814, 1487, 2002
BssT1I CCWWGG 2 cut(s) 123, 2410
Bst2UI CCWGG 6 cut(s) 276, 402, 527, 1938, 2325, 2560
Bst4CI ACNGT 9 cut(s) 364, 504, 1152, 1429, 1615, 1652, 1706, 2232, 2393
Bst6I CTCTTC 4 cut(s) 466, 1002, 2247, 2659
BstAPI GCANNNNNTGC 1 cut(s) 766
BstBAI YACGTR 1 cut(s) 1412
BstBI TTCGAA 1 cut(s) 1872
BstC8I GCNNGC 4 cut(s) 75, 113, 1337, 1794
BstDEI CTNAG 6 cut(s) 516, 591, 836, 1853, 1889, 2534
BstFNI CGCG 1 cut(s) 2467
BstHHI GCGC 2 cut(s) 1104, 2469
BstKTI GATC 5 cut(s) 39, 681, 817, 1490, 2005
BstMAI GTCTC 3 cut(s) 428, 658, 2656
BstMBI GATC 5 cut(s) 36, 678, 814, 1487, 2002
BstMCI CGRYCG 1 cut(s) 1343
BstMWI GCNNNNNNNGC 7 cut(s) 202, 224, 596, 766, 2081, 2255, 2466
BstNI CCWGG 6 cut(s) 276, 402, 527, 1938, 2325, 2560
BstNSI RCATGY 3 cut(s) 122, 2156, 2508
BstPAI GACNNNNGTC 1 cut(s) 650
BstSCI CCNGG 7 cut(s) 274, 400, 525, 1936, 2196, 2323, 2558
BstSFI CTRYAG 1 cut(s) 2259
BstUI CGCG 1 cut(s) 2467
BstV1I GCAGC 7 cut(s) 227, 238, 753, 950, 1088, 1434, 2656
BstV2I GAAGAC 1 cut(s) 1353
BstX2I RGATCY 1 cut(s) 1487
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 1 cut(s) 1487
BsuI GTATCC 2 cut(s) 1026, 1427
BsuRI GGCC 4 cut(s) 1509, 1887, 2201, 2277
BtsI GCAGTG 1 cut(s) 1967
BtsIMutI CAGTG 2 cut(s) 1967, 2542
Cac8I GCNNGC 4 cut(s) 75, 113, 1337, 1794
CaiI CAGNNNCTG 2 cut(s) 112, 1969
CciI TCATGA 4 cut(s) 220, 681, 909, 1456
CfoI GCGC 2 cut(s) 1104, 2469
Cfr10I RCCGGY 2 cut(s) 1792, 1883
Cfr13I GGNCC 2 cut(s) 272, 294
CseI GACGC 1 cut(s) 2473
CsiI ACCWGGT 1 cut(s) 2558
Csp6I GTAC 4 cut(s) 1067, 2012, 2228, 2496
CviQI GTAC 4 cut(s) 1067, 2012, 2228, 2496
DdeI CTNAG 6 cut(s) 516, 591, 836, 1853, 1889, 2534
DpnI GATC 5 cut(s) 38, 680, 816, 1489, 2004
DpnII GATC 5 cut(s) 36, 678, 814, 1487, 2002
DraI TTTAAA 2 cut(s) 1108, 1830
EaeI YGGCCR 1 cut(s) 2199
Eam1104I CTCTTC 4 cut(s) 466, 1002, 2247, 2659
EarI CTCTTC 4 cut(s) 466, 1002, 2247, 2659
Ecl136II GAGCTC 1 cut(s) 2084
Eco130I CCWWGG 2 cut(s) 123, 2410
Eco147I AGGCCT 1 cut(s) 1509
Eco24I GRGCYC 1 cut(s) 2086
Eco32I GATATC 2 cut(s) 82, 782
Eco47I GGWCC 2 cut(s) 272, 294
Eco53kI GAGCTC 1 cut(s) 2084
Eco57I CTGAAG 4 cut(s) 831, 1946, 2120, 2271
EcoICRI GAGCTC 1 cut(s) 2084
EcoO109I RGGNCCY 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 1271
EcoRII CCWGG 6 cut(s) 274, 400, 525, 1936, 2323, 2558
EcoRV GATATC 2 cut(s) 82, 782
EcoT14I CCWWGG 2 cut(s) 123, 2410
EcoT22I ATGCAT 2 cut(s) 1500, 2587
EcoT38I GRGCYC 1 cut(s) 2086
ErhI CCWWGG 2 cut(s) 123, 2410
FalI AAGNNNNNCTT 6 cut(s) 129, 161, 738, 770, 2100, 2132
FaqI GGGAC 4 cut(s) 101, 842, 887, 2179
FauI CCCGC 3 cut(s) 606, 896, 2665
Fnu4HI GCNGC 8 cut(s) 216, 252, 767, 964, 1077, 1423, 1540, 2670
FriOI GRGCYC 1 cut(s) 2086
Fsp4HI GCNGC 8 cut(s) 216, 252, 767, 964, 1077, 1423, 1540, 2670
FspBI CTAG 5 cut(s) 438, 1322, 2015, 2186, 2306
GlaI GCGC 2 cut(s) 1103, 2468
GluI GCNGC 8 cut(s) 216, 252, 767, 964, 1077, 1423, 1540, 2670
GsaI CCCAGC 1 cut(s) 970
GsuI CTGGAG 2 cut(s) 89, 2100
HaeIII GGCC 4 cut(s) 1509, 1887, 2201, 2277
HapII CCGG 5 cut(s) 187, 1343, 1793, 1884, 2198
HgaI GACGC 1 cut(s) 2473
HhaI GCGC 2 cut(s) 1104, 2469
Hin6I GCGC 2 cut(s) 1102, 2467
HinP1I GCGC 2 cut(s) 1102, 2467
HincII GTYRAC 2 cut(s) 2607, 2629
HindII GTYRAC 2 cut(s) 2607, 2629
HindIII AAGCTT 1 cut(s) 71
HpaI GTTAAC 1 cut(s) 2607
HpaII CCGG 5 cut(s) 187, 1343, 1793, 1884, 2198
HphI GGTGA 5 cut(s) 12, 515, 1367, 2012, 2501
HpyAV CCTTC 7 cut(s) 88, 245, 937, 1157, 1322, 1409, 1520
HpyCH4III ACNGT 9 cut(s) 364, 504, 1152, 1429, 1615, 1652, 1706, 2232, 2393
HpyCH4IV ACGT 2 cut(s) 9, 1411
HpyF10VI GCNNNNNNNGC 7 cut(s) 202, 224, 596, 766, 2081, 2255, 2466
HpyF3I CTNAG 6 cut(s) 516, 591, 836, 1853, 1889, 2534
HpySE526I ACGT 2 cut(s) 9, 1411
HspAI GCGC 2 cut(s) 1102, 2467
KroI GCCGGC 1 cut(s) 1792
KroNI GCCGGC 1 cut(s) 1794
KspAI GTTAAC 1 cut(s) 2607
Kzo9I GATC 5 cut(s) 36, 678, 814, 1487, 2002
LguI GCTCTTC 1 cut(s) 466
LmnI GCTCC 4 cut(s) 160, 1253, 2081, 2666
Lsp1109I GCAGC 7 cut(s) 227, 238, 753, 950, 1088, 1434, 2656
LweI GCATC 8 cut(s) 62, 142, 214, 310, 330, 857, 1157, 2236
MabI ACCWGGT 1 cut(s) 2558
MaeI CTAG 5 cut(s) 438, 1322, 2015, 2186, 2306
MaeII ACGT 2 cut(s) 9, 1411
MaeIII GTNAC 8 cut(s) 504, 521, 703, 734, 1407, 1570, 1950, 2461
MalI GATC 5 cut(s) 38, 680, 816, 1489, 2004
MbiI CCGCTC 2 cut(s) 613, 1542
MboI GATC 5 cut(s) 36, 678, 814, 1487, 2002
MfeI CAATTG 1 cut(s) 2380
MflI RGATCY 1 cut(s) 1487
MhlI GDGCHC 1 cut(s) 2086
MlyI GAGTC 7 cut(s) 464, 577, 707, 911, 1866, 2341, 2428
MmeI TCCRAC 3 cut(s) 646, 774, 1411
Mph1103I ATGCAT 2 cut(s) 1500, 2587
MroNI GCCGGC 1 cut(s) 1792
MroXI GAANNNNTTC 1 cut(s) 2400
MseI TTAA 7 cut(s) 387, 1107, 1316, 1667, 1829, 1944, 2606
MslI CAYNNNNRTG 3 cut(s) 266, 528, 630
MspI CCGG 5 cut(s) 187, 1343, 1793, 1884, 2198
MspR9I CCNGG 7 cut(s) 276, 402, 527, 1938, 2198, 2325, 2560
MunI CAATTG 1 cut(s) 2380
Mva1269I GAATGC 1 cut(s) 1241
MvaI CCWGG 6 cut(s) 276, 402, 527, 1938, 2325, 2560
MvnI CGCG 1 cut(s) 2467
MwoI GCNNNNNNNGC 7 cut(s) 202, 224, 596, 766, 2081, 2255, 2466
NaeI GCCGGC 1 cut(s) 1794
NciI CCSGG 1 cut(s) 2198
NdeII GATC 5 cut(s) 36, 678, 814, 1487, 2002
NgoMIV GCCGGC 1 cut(s) 1792
NlaIV GGNNCC 2 cut(s) 273, 1489
NmeAIII GCCGAG 1 cut(s) 730
NmuCI GTSAC 3 cut(s) 521, 734, 2461
NsiI ATGCAT 2 cut(s) 1500, 2587
NspI RCATGY 3 cut(s) 122, 2156, 2508
NspV TTCGAA 1 cut(s) 1872
PagI TCATGA 4 cut(s) 220, 681, 909, 1456
PceI AGGCCT 1 cut(s) 1509
PciI ACATGT 1 cut(s) 2152
PciSI GCTCTTC 1 cut(s) 466
PctI GAATGC 1 cut(s) 1241
PdiI GCCGGC 1 cut(s) 1794
PdmI GAANNNNTTC 1 cut(s) 2400
PfeI GAWTC 4 cut(s) 1807, 2026, 2328, 2416
PflMI CCANNNNNTGG 1 cut(s) 2411
PfoI TCCNGGA 2 cut(s) 274, 2323
PkrI GCNGC 8 cut(s) 217, 253, 768, 965, 1078, 1424, 1541, 2671
PleI GAGTC 7 cut(s) 463, 577, 706, 911, 1865, 2341, 2428
PpsI GAGTC 7 cut(s) 463, 577, 706, 911, 1865, 2341, 2428
Ppu21I YACGTR 1 cut(s) 1412
PpuMI RGGWCCY 1 cut(s) 272
PscI ACATGT 1 cut(s) 2152
PshAI GACNNNNGTC 1 cut(s) 650
PsiI TTATAA 1 cut(s) 1557
Psp124BI GAGCTC 1 cut(s) 2086
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 6 cut(s) 274, 400, 525, 1936, 2323, 2558
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 6 cut(s) 274, 400, 525, 1936, 2323, 2558
PspN4I GGNNCC 2 cut(s) 273, 1489
PspPI GGNCC 2 cut(s) 272, 294
PspPPI RGGWCCY 1 cut(s) 272
PstNI CAGNNNCTG 2 cut(s) 112, 1969
PsuI RGATCY 1 cut(s) 1487
RsaI GTAC 4 cut(s) 1068, 2013, 2229, 2497
RsaNI GTAC 4 cut(s) 1067, 2012, 2228, 2496
RseI CAYNNNNRTG 3 cut(s) 266, 528, 630
SacI GAGCTC 1 cut(s) 2086
SapI GCTCTTC 1 cut(s) 466
SaqAI TTAA 7 cut(s) 387, 1107, 1316, 1667, 1829, 1944, 2606
SatI GCNGC 8 cut(s) 216, 252, 767, 964, 1077, 1423, 1540, 2670
Sau3AI GATC 5 cut(s) 36, 678, 814, 1487, 2002
Sau96I GGNCC 2 cut(s) 272, 294
SchI GAGTC 7 cut(s) 464, 577, 707, 911, 1866, 2341, 2428
ScrFI CCNGG 7 cut(s) 276, 402, 527, 1938, 2198, 2325, 2560
SduI GDGCHC 1 cut(s) 2086
SexAI ACCWGGT 1 cut(s) 2558
SfaNI GCATC 8 cut(s) 62, 142, 214, 310, 330, 857, 1157, 2236
SfcI CTRYAG 1 cut(s) 2259
SfuI TTCGAA 1 cut(s) 1872
SinI GGWCC 2 cut(s) 272, 294
SmiMI CAYNNNNRTG 3 cut(s) 266, 528, 630
SmlI CTYRAG 3 cut(s) 206, 2117, 2596
SmoI CTYRAG 3 cut(s) 206, 2117, 2596
SseBI AGGCCT 1 cut(s) 1509
SsiI CCGC 4 cut(s) 613, 889, 1540, 2672
SspI AATATT 1 cut(s) 2047
SspMI CTAG 5 cut(s) 438, 1322, 2015, 2186, 2306
SstI GAGCTC 1 cut(s) 2086
StuI AGGCCT 1 cut(s) 1509
StyD4I CCNGG 7 cut(s) 274, 400, 525, 1936, 2196, 2323, 2558
StyI CCWWGG 2 cut(s) 123, 2410
TaaI ACNGT 9 cut(s) 364, 504, 1152, 1429, 1615, 1652, 1706, 2232, 2393
TaiI ACGT 2 cut(s) 12, 1414
TaqI TCGA 5 cut(s) 394, 1228, 1761, 1872, 2564
TatI WGTACW 2 cut(s) 2011, 2495
TauI GCSGC 1 cut(s) 1542
TfiI GAWTC 4 cut(s) 1807, 2026, 2328, 2416
Tru1I TTAA 7 cut(s) 387, 1107, 1316, 1667, 1829, 1944, 2606
Tru9I TTAA 7 cut(s) 387, 1107, 1316, 1667, 1829, 1944, 2606
TscAI CASTG 2 cut(s) 1974, 2542
TseFI GTSAC 3 cut(s) 521, 734, 2461
TseI GCWGC 7 cut(s) 215, 251, 766, 963, 1076, 1422, 2669
Tsp45I GTSAC 3 cut(s) 521, 734, 2461
TspGWI ACGGA 2 cut(s) 1480, 2463
TspRI CASTG 2 cut(s) 1974, 2542
Van91I CCANNNNNTGG 1 cut(s) 2411
VpaK11BI GGWCC 2 cut(s) 272, 294
XapI RAATTY 6 cut(s) 1179, 1271, 1533, 2020, 2265, 2293
XbaI TCTAGA 1 cut(s) 1321
XceI RCATGY 3 cut(s) 122, 2156, 2508
XmnI GAANNNNTTC 1 cut(s) 2400
XspI CTAG 5 cut(s) 438, 1322, 2015, 2186, 2306
Zsp2I ATGCAT 2 cut(s) 1500, 2587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.