Rh6AG166200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000400
Physical Location & Seq
Reverse (-)
26561893 .. 26565498
3606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG166200.1

Sequence Viewer

Length: 3606 bp
ATGGAAGCAGCTGCAAGCATAGTTTTATCTCCTGCTTTGCAAGTGCTGTTTGACAGACTAGCATCCCCGCTCCTCGAAAGGACTGCTGATATTTTCGGTTTCAAGGACAACCTCCGCAGCCTACGACATGCATTGTCAAGGGCTCAAGCTACCCTGGAGGATGCAGAAGAGCAACAATTCACCAGCAAAGCCATTAGACTTTGGCTGTCTGACCTCAAGAATGCAGCCATGGATGCACAGGATCTTCTGGAGTTCTTCATTGCTCATGAAACTTTGGAGAAAGAGACCGGCAAGGATGTTCCTCAGGTTTTGCTTACTGCTTCTAATCATCCGGACAATGTCAGAAAGGTTCTTCAGGCCTTAGAACTGAGTCTATCAGAGGGATTTCCACTGTTGAGTGTTAGAGATCCTGCCCTGCCTCGTATGGCCGATCGAAGATATGATAACAGGGAGACTAATTCCTTTTTTGTGGACTCGAAGATATATGGAAGAGATGATGACAAAGACAGGTTAGTCCAACTCTTGCTATCTTCTGCTGCAACCAGCCAGGTGAGTCAACAGGAATATGGATGTGTTGCAGCGTGCATTCCAATAATCGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAACTGACATATAATGATACGAGCATAATCCAACACTTCGATCTTAGGGTGTGGATTTCTGTCTCTACAGTCTTCAATGTGAAACATATACTGCAGACCATTGTTGCTTCTGCAACAAAGGAAGAGTCCAAGCTTTCAAAGATCCGGTCGATGGAAAATTTGCTTTATGAATCTTTACGCAACAAAAGGTATCTGATCGTATTAGATGATGTCTGGACGGAAGACCAAGATGACTGGGACAAATTAAGACCTTTGTTTACAGCGGGTCTGGATGGATGCAAAATCATTGTCACCACCCGCAGTAAGAAAATCCCATTCATGATGTACTTCCCAAATTATCCATATCATTTGAAGGGTTTGGCAGAGGATGATTGCTGGGCTGTGTTCAAACACCGGGCATTTGGATTAGGCGAAGAAGTCAATTATCCAAACCTTGTGCTGATTGGAAAGCAGATTGTCAAAAAATGTGGAGGTATACCTTTAGCAGCAAAAAGTATGGGAAGTGCATTGCGCTTGAAGAGACAGGAAAGGCAGTGGTTGTTCATGCGAGATTGCGAACTTTGGAAATTAGATGCAAGCCAGCATAAAGTTCTACCTGCTCTCATGCTGAGTTATCATCATCTACCATCTCATCTTCGACAATGTTTTGAATTCTGCTCATTGTTTCCCAAAAATTATGAGTTCAAGAAGCAGCGATTAATTCATCTATGGATGGCAGGAGGCTTAATTCTACAAGAAGGAAACAAGCGACCAGAAGACATTGGAGATGAGTACTTTGATGATTTGTTGTGGATGTCTTTCTTTGAAGAAGTAGAGCAATGTGATCATGGCAGAACAGCAATTGCATACAAAATGATTGACATCATTCATGATCTTGCACGATATGTTGCAGGTAAAGAATATGTGATCCTTGAACATGATCTTTCACTACATAGTCCAACACAAATTCGCCACTCATCTCTTGTTCCTAAAGGGGAAATCAGAATTCCAGAAGCTCTGTATGAAGCTGAACATTTGCGGTCTCTCTTAGTCATTGGAGGTTTTGGCACATTCAACAACCAAAGCAAATTATTTTCAAGTTTTGTATACCTGCGTATGCTAGACTTGAAGAGCTGTGATATAGATGATTTGCCAAGATCACTGGGTGGCTTGATATGTTTGAGGTATCTTGACATGTCTTACACACGCATCTCCAGGTTACCTGAAAGCACAAGTAATCTCTGTTCTTTGCAGACTTTAAACCTTGTTGGTTGTGGTCGCCTTAAAGTGTTACCCTTTTTGGGAAATATGATCAACCTGAGACATCTCAATATAACCGGATGTAATCGTTTGAATGAGATGCCTTTTGGTATTCAAAGATTACTTCAACTTCAGATATTGCCATTGTGTGTTGTCAGCAGGAACCAAAGGGCGCTCACTATGCTTGAACATTTGAATCTTTATGGCGAGTTGAATATCACCTACTTGGAGAACACAAGCTCTCCAGATGAAGCACAGTCAGCTCGATTGAATATGAAGGAAAATCTTGAGTCGGTGGGATTACATTGGGGATCCCAACCTCTGGATCAATCATTTCCGAGACTATTAGGTAAATCCCGACATGATGTAGTCATCTCCAGCTCTCAAGTAGCGCCACAGCTGTGTAAACGTGTGGAAGAAGTTCTTGACGGTCTGCAGCCACACAATAATCTGAAAAAGCTAGTTATAAATGGCTATCCGGGAATTAGATTTGCTGATTGGGCTCTCCCAAAAATGCTGATTACAGTTGATTTGATCAACTGTACAAATTGTCAACATCTTCCAGCCCTTGGGAATCTTCCGCTGCTCAAGACACTCTATATGCATGGAATGAATGGCGTCAAGTGCATTGGTACAGAGTTCTATGGTGACGGTACTGACGTAAGGTTTCCATCTCTGAAAGAACTATCTCTCAGTCATTTTCCAAATTTGGAACAATGGTCATGTGCAGATGATGGAAATGCATTTCCTCATTTGAGCAAGTTGACAGTTAGAAGCTGCCCCAAGTTACAACAGATATCAGTATCTCAGTCTCTTCAACATTTGGAGCTACGGGATTGCAACCCAACATTGATGTGCATAGAAAATTTAACTTTGCTTTCTGTTCTTGTAATAGAAAAACTTCCAGAGCTAACCTCTCTCCCAGAAGGGCTTTTTGTATCAACTCGTCTGTCGTCTCTGGAGATCCTGTCATGTCCTAAGCTTCGTTCACTGCCTTTGAAGATTGGAAACCTTGCTGCTCTGAAATCATTGACCATTCGATGGTGTGAAGAGCTATCCACCCTACCCCAGAGTATAGAGGACCTCAAAGCTCTGGAGTCACTGGAGATTAGTAACTGTAATAATATAATCTCCATGCCGGATGGTGGAATTGGAGGTCTGAGTTCCCTTCGAACTTTGTCTATTGAAAACTGCAATAATCTGATTTCTTTGTCATCGAGTTTGGAGAATCTCACATTCCTTGAGCACTTGGTCATTATGTATTGTCCAAATCTTACTTCTTTCCCAGAGGGCACGGGCGCGCAACCTCTCTCTGCCCTACGAAGTTTGACTATCTTGGGTTGTCCTTGGTTTGATTCTCTACCAAATGAATTGCAAAATCTCAGGACGCTACAATGCCTGGAAATTGGTGGCTGCCCGAATCTAACTGCTTTGCCAGACTGGTTTGAGAAACTGGATTCTCTCCGATCCTTGACCATTTGCGATTGTCCCAATTTAATGGTACTGCCGCCCTGTCTAACGCTTCTCACTAAACTCCAGCATCTCTCCATTCAAGATTGTCCTCAGCTCGAAGAAAGATGTAGACGGGGAATTGGTGAGGATTGGTTCAATATAGCACATGTGCCCCATCAATACATTGGTCCAAGTCAGCCATACATTGGTCCAAATCAGGTGACTGAGGTCGGGCTGTCGTATTCTTACCCTATGCCAAAAACTGCATCACACTACACTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1201

Amino Acids

135.48

Weight (kDa)

6.11

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 3.2e-17 Rx N-terminal domain
NB-ARC PF00931 195 - 344 6.7e-35 NB-ARC domain
WHD_DRP PF23559 431 - 503 8.3e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 548 - 898 1.4e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 683 - 827 8.5e-28 R13L1/DRL21 LRRs
LRR_14 PF23598 946 - 1043 3.4e-09 Leucine-rich repeat region
LRR_14 PF23598 1060 - 1143 1.9e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2336
AasI GACNNNNNNGTC 1 cut(s) 512
Acc36I ACCTGC 3 cut(s) 1234, 1511, 1728
AccB7I CCANNNNNTGG 4 cut(s) 2191, 2438, 2910, 3527
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 3 cut(s) 1105, 1716, 3451
AccII CGCG 1 cut(s) 3170
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 7 cut(s) 68, 115, 893, 928, 1648, 2450, 3377
AclWI GGATC 9 cut(s) 249, 401, 766, 1531, 2175, 2188, 2202, 2827, 3330
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 6 cut(s) 787, 1280, 1575, 1614, 2575, 2734
AcuI CTGAAG 2 cut(s) 338, 1985
AcyI GRCGYC 1 cut(s) 2487
AdeI CACNNNGTG 1 cut(s) 1775
AfaI GTAC 6 cut(s) 956, 1403, 2413, 2503, 2524, 3372
AfiI CCNNNNNNNGG 8 cut(s) 596, 781, 1910, 2191, 2438, 2910, 3014, 3527
AflIII ACRYGT 3 cut(s) 1803, 2278, 3487
AjnI CCWGG 4 cut(s) 153, 546, 1823, 3267
AjuI GAANNNNNNNTTGG 2 cut(s) 3130, 3162
AleI CACNNNNGTG 1 cut(s) 2269
Alw21I GWGCWC 1 cut(s) 3117
Alw26I GTCTC 9 cut(s) 278, 446, 697, 1144, 1656, 1924, 2203, 2685, 2829
AlwI GGATC 9 cut(s) 249, 401, 766, 1531, 2175, 2188, 2202, 2827, 3330
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 2 cut(s) 357, 426
ApoI RAATTY 6 cut(s) 787, 1280, 1575, 1614, 2575, 2734
ArsI GACNNNNNNTTYG 6 cut(s) 740, 761, 772, 793, 903, 935
AseI ATTAAT 1 cut(s) 1328
Asp700I GAANNNNTTC 2 cut(s) 2289, 2769
AspLEI GCGC 5 cut(s) 1143, 2044, 2263, 3170, 3172
AspS9I GGNCC 3 cut(s) 2950, 3509, 3530
AsuC2I CCSGG 2 cut(s) 1025, 2349
AsuHPI GGTGA 7 cut(s) 172, 562, 913, 2080, 2528, 3476, 3553
AsuII TTCGAA 1 cut(s) 3040
AvaII GGWCC 3 cut(s) 2950, 3509, 3530
AxyI CCTNAGG 1 cut(s) 303
BaeGI GKGCMC 2 cut(s) 3164, 3495
BaeI ACNNNNGTAYC 2 cut(s) 2514, 2547
BamHI GGATCC 1 cut(s) 2180
BanII GRGCYC 2 cut(s) 145, 2374
BbsI GAAGAC 3 cut(s) 694, 858, 1392
Bbv12I GWGCWC 1 cut(s) 3117
BbvCI CCTCAGC 1 cut(s) 3432
BccI CCATC 9 cut(s) 775, 896, 1264, 1336, 2548, 2597, 2904, 3005, 3504
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 4 cut(s) 155, 548, 1825, 3269
BclI TGATCA 3 cut(s) 1453, 1920, 2403
BcnI CCSGG 2 cut(s) 1025, 2349
BcoDI GTCTC 9 cut(s) 278, 446, 697, 1144, 1656, 1924, 2203, 2685, 2829
BfaI CTAG 3 cut(s) 59, 1730, 2330
BfmI CTRYAG 3 cut(s) 696, 722, 2303
BfoI RGCGCY 2 cut(s) 2045, 2264
BfuAI ACCTGC 3 cut(s) 1234, 1511, 1728
BmcAI AGTACT 1 cut(s) 1403
Bme1390I CCNGG 6 cut(s) 155, 548, 1025, 1825, 2349, 3269
Bme18I GGWCC 3 cut(s) 2950, 3509, 3530
BmgT120I GGNCC 3 cut(s) 2950, 3509, 3530
BmiI GGNNCC 2 cut(s) 2033, 2182
BmrFI CCNGG 6 cut(s) 155, 548, 1025, 1825, 2349, 3269
BmrI ACTGGG 2 cut(s) 874, 1781
BmsI GCATC 9 cut(s) 71, 151, 223, 896, 1192, 1827, 1959, 3418, 3596
BmuI ACTGGG 2 cut(s) 874, 1781
BoxI GACNNNNGTC 1 cut(s) 3548
BpiI GAAGAC 3 cut(s) 694, 858, 1392
BplI GAGNNNNNCTC 2 cut(s) 2768, 2800
BpmI CTGGAG 9 cut(s) 176, 269, 1807, 2097, 2230, 2849, 2984, 2993, 3389
Bpu10I CCTNAGC 2 cut(s) 2847, 3432
Bpu14I TTCGAA 1 cut(s) 3040
BpuEI CTTGAG 6 cut(s) 129, 200, 2177, 2238, 2441, 3131
BpuMI CCSGG 2 cut(s) 1025, 2349
BsaBI GATNNNNATC 1 cut(s) 1490
BsaHI GRCGYC 1 cut(s) 2487
BsaI GGTCTC 2 cut(s) 278, 1656
BsaJI CCNNGG 4 cut(s) 153, 228, 2437, 3215
BsaWI WCCGGW 3 cut(s) 331, 774, 1946
BsaXI ACNNNNNCTCC 4 cut(s) 2095, 2125, 2822, 2852
Bsc4I CCNNNNNNNGG 8 cut(s) 596, 781, 1910, 2191, 2438, 2910, 3014, 3527
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 5 cut(s) 869, 1776, 2976, 3314, 3327
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 3 cut(s) 258, 1136, 1454
Bse8I GATNNNNATC 1 cut(s) 1490
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 4 cut(s) 155, 548, 1825, 3269
BseDI CCNNGG 4 cut(s) 153, 228, 2437, 3215
BseJI GATNNNNATC 1 cut(s) 1490
BseLI CCNNNNNNNGG 8 cut(s) 596, 781, 1910, 2191, 2438, 2910, 3014, 3527
BseMI GCAATG 3 cut(s) 258, 1136, 1454
BseNI ACTGG 5 cut(s) 869, 1776, 2976, 3314, 3327
BsePI GCGCGC 1 cut(s) 3168
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 2 cut(s) 3164, 3495
BseYI CCCAGC 1 cut(s) 1005
BsgI GTGCAG 1 cut(s) 2616
Bsh1236I CGCG 1 cut(s) 3170
Bsh1285I CGRYCG 2 cut(s) 433, 779
BshFI GGCC 2 cut(s) 359, 428
BsiEI CGRYCG 2 cut(s) 433, 779
BsiHKAI GWGCWC 1 cut(s) 3117
BsiSI CCGG 7 cut(s) 288, 332, 775, 1024, 1947, 2348, 3008
BslFI GGGAC 2 cut(s) 881, 3342
BslI CCNNNNNNNGG 8 cut(s) 596, 781, 1910, 2191, 2438, 2910, 3014, 3527
BsmAI GTCTC 9 cut(s) 278, 446, 697, 1144, 1656, 1924, 2203, 2685, 2829
BsmBI CGTCTC 1 cut(s) 2829
BsmFI GGGAC 2 cut(s) 881, 3342
BsmI GAATGC 2 cut(s) 226, 585
BsnI GGCC 2 cut(s) 359, 428
Bso31I GGTCTC 2 cut(s) 278, 1656
Bsp119I TTCGAA 1 cut(s) 3040
Bsp1286I GDGCHC 5 cut(s) 145, 2374, 3117, 3164, 3495
Bsp13I TCCGGA 1 cut(s) 331
Bsp1407I TGTACA 1 cut(s) 2411
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 7 cut(s) 68, 115, 893, 928, 1648, 2450, 3377
BspANI GGCC 2 cut(s) 359, 428
BspEI TCCGGA 1 cut(s) 331
BspFNI CGCG 1 cut(s) 3170
BspHI TCATGA 3 cut(s) 265, 948, 1498
BspLI GGNNCC 2 cut(s) 2033, 2182
BspMAI CTGCAG 2 cut(s) 726, 2307
BspMI ACCTGC 3 cut(s) 1234, 1511, 1728
BspPI GGATC 9 cut(s) 249, 401, 766, 1531, 2175, 2188, 2202, 2827, 3330
BspQI GCTCTTC 3 cut(s) 162, 1733, 2913
BspT104I TTCGAA 1 cut(s) 3040
BspTNI GGTCTC 2 cut(s) 278, 1656
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 3 cut(s) 258, 1136, 1454
BsrFI RCCGGY 1 cut(s) 287
BsrGI TGTACA 1 cut(s) 2411
BsrI ACTGG 5 cut(s) 869, 1776, 2976, 3314, 3327
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 4 cut(s) 153, 228, 2437, 3215
BssHII GCGCGC 1 cut(s) 3168
BssNAI GTATAC 2 cut(s) 1106, 1717
BssNI GRCGYC 1 cut(s) 2487
BssT1I CCWWGG 3 cut(s) 228, 2437, 3215
Bst1107I GTATAC 2 cut(s) 1106, 1717
Bst2UI CCWGG 4 cut(s) 155, 548, 1825, 3269
Bst4CI ACNGT 9 cut(s) 393, 700, 2127, 2300, 2395, 2411, 2522, 2638, 2987
Bst6I CTCTTC 7 cut(s) 162, 484, 747, 1142, 1733, 2688, 2913
BstACI GRCGYC 1 cut(s) 2487
BstAUI TGTACA 1 cut(s) 2411
BstBI TTCGAA 1 cut(s) 3040
BstC8I GCNNGC 5 cut(s) 16, 583, 1207, 1211, 3170
BstDSI CCRYGG 1 cut(s) 228
BstEII GGTNACC 1 cut(s) 1827
BstFNI CGCG 1 cut(s) 3170
BstH2I RGCGCY 2 cut(s) 2045, 2264
BstHHI GCGC 5 cut(s) 1143, 2044, 2263, 3170, 3172
BstMAI GTCTC 9 cut(s) 278, 446, 697, 1144, 1656, 1924, 2203, 2685, 2829
BstMCI CGRYCG 2 cut(s) 433, 779
BstMWI GCNNNNNNNGC 5 cut(s) 233, 2050, 2129, 2369, 2493
BstNI CCWGG 4 cut(s) 155, 548, 1825, 3269
BstNSI RCATGY 3 cut(s) 131, 1807, 3491
BstPAI GACNNNNGTC 1 cut(s) 3548
BstPI GGTNACC 1 cut(s) 1827
BstSCI CCNGG 6 cut(s) 153, 546, 1023, 1823, 2347, 3267
BstSFI CTRYAG 3 cut(s) 696, 722, 2303
BstSLI GKGCMC 2 cut(s) 3164, 3495
BstUI CGCG 1 cut(s) 3170
BstV2I GAAGAC 3 cut(s) 694, 858, 1392
BstX2I RGATCY 5 cut(s) 241, 406, 771, 2180, 2832
BstXI CCANNNNNNTGG 1 cut(s) 3367
BstYI RGATCY 5 cut(s) 241, 406, 771, 2180, 2832
BstZ17I GTATAC 2 cut(s) 1106, 1717
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 2 cut(s) 359, 428
BtgI CCRYGG 1 cut(s) 228
BtsI GCAGTG 2 cut(s) 1169, 2858
BtsIMutI CAGTG 5 cut(s) 389, 1169, 1769, 2858, 2969
BveI ACCTGC 3 cut(s) 1234, 1511, 1728
Cac8I GCNNGC 5 cut(s) 16, 583, 1207, 1211, 3170
CciI TCATGA 3 cut(s) 265, 948, 1498
CfoI GCGC 5 cut(s) 1143, 2044, 2263, 3170, 3172
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 3 cut(s) 2950, 3509, 3530
CseI GACGC 2 cut(s) 2476, 3265
Csp6I GTAC 6 cut(s) 955, 1402, 2412, 2502, 2523, 3371
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviQI GTAC 6 cut(s) 955, 1402, 2412, 2502, 2523, 3371
DraI TTTAAA 1 cut(s) 1869
DraIII CACNNNGTG 1 cut(s) 1775
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 7 cut(s) 162, 484, 747, 1142, 1733, 2688, 2913
EarI CTCTTC 7 cut(s) 162, 484, 747, 1142, 1733, 2688, 2913
Eco130I CCWWGG 3 cut(s) 228, 2437, 3215
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 2 cut(s) 145, 2374
Eco31I GGTCTC 2 cut(s) 278, 1656
Eco32I GATATC 1 cut(s) 2667
Eco47I GGWCC 3 cut(s) 2950, 3509, 3530
Eco57I CTGAAG 2 cut(s) 338, 1985
Eco81I CCTNAGG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 1827
EcoO109I RGGNCCY 1 cut(s) 2950
EcoO65I GGTNACC 1 cut(s) 1827
EcoRI GAATTC 2 cut(s) 1280, 1614
EcoRII CCWGG 4 cut(s) 153, 546, 1823, 3267
EcoRV GATATC 1 cut(s) 2667
EcoT14I CCWWGG 3 cut(s) 228, 2437, 3215
EcoT22I ATGCAT 3 cut(s) 133, 2475, 2614
EcoT38I GRGCYC 2 cut(s) 145, 2374
ErhI CCWWGG 3 cut(s) 228, 2437, 3215
Esp3I CGTCTC 1 cut(s) 2829
FalI AAGNNNNNCTT 2 cut(s) 2277, 2309
FaqI GGGAC 2 cut(s) 881, 3342
FauI CCCGC 3 cut(s) 75, 886, 935
FbaI TGATCA 3 cut(s) 1453, 1920, 2403
FblI GTMKAC 3 cut(s) 1105, 1716, 3451
FriOI GRGCYC 2 cut(s) 145, 2374
FspBI CTAG 3 cut(s) 59, 1730, 2330
GlaI GCGC 5 cut(s) 1142, 2043, 2262, 3169, 3171
GsaI CCCAGC 1 cut(s) 1009
GsuI CTGGAG 9 cut(s) 176, 269, 1807, 2097, 2230, 2849, 2984, 2993, 3389
HaeII RGCGCY 2 cut(s) 2045, 2264
HaeIII GGCC 2 cut(s) 359, 428
HapII CCGG 7 cut(s) 288, 332, 775, 1024, 1947, 2348, 3008
HgaI GACGC 2 cut(s) 2476, 3265
HhaI GCGC 5 cut(s) 1143, 2044, 2263, 3170, 3172
Hin1I GRCGYC 1 cut(s) 2487
Hin6I GCGC 5 cut(s) 1141, 2042, 2261, 3168, 3170
HinP1I GCGC 5 cut(s) 1141, 2042, 2261, 3168, 3170
HincII GTYRAC 3 cut(s) 557, 2423, 2634
HindII GTYRAC 3 cut(s) 557, 2423, 2634
HindIII AAGCTT 2 cut(s) 761, 2849
HpaII CCGG 7 cut(s) 288, 332, 775, 1024, 1947, 2348, 3008
HphI GGTGA 7 cut(s) 172, 562, 913, 2080, 2528, 3476, 3553
HpyAV CCTTC 5 cut(s) 976, 1361, 2140, 2789, 3047
HpyCH4III ACNGT 9 cut(s) 393, 700, 2127, 2300, 2395, 2411, 2522, 2638, 2987
HpyCH4IV ACGT 2 cut(s) 2278, 2529
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 2050, 2129, 2369, 2493
HpySE526I ACGT 2 cut(s) 2278, 2529
Hsp92I GRCGYC 1 cut(s) 2487
HspAI GCGC 5 cut(s) 1141, 2042, 2261, 3168, 3170
Kpn2I TCCGGA 1 cut(s) 331
Ksp22I TGATCA 3 cut(s) 1453, 1920, 2403
LguI GCTCTTC 3 cut(s) 162, 1733, 2913
LmnI GCTCC 2 cut(s) 75, 2695
LweI GCATC 9 cut(s) 71, 151, 223, 896, 1192, 1827, 1959, 3418, 3596
MaeI CTAG 3 cut(s) 59, 1730, 2330
MaeII ACGT 2 cut(s) 2278, 2529
MaeIII GTNAC 8 cut(s) 919, 1827, 1899, 2516, 2655, 2967, 2981, 3541
MbiI CCGCTC 1 cut(s) 70
MfeI CAATTG 1 cut(s) 1470
MflI RGATCY 5 cut(s) 241, 406, 771, 2180, 2832
MhlI GDGCHC 5 cut(s) 145, 2374, 3117, 3164, 3495
MlyI GAGTC 6 cut(s) 379, 467, 562, 764, 2168, 2975
MmeI TCCRAC 3 cut(s) 541, 685, 1592
Mph1103I ATGCAT 3 cut(s) 133, 2475, 2614
MroI TCCGGA 1 cut(s) 331
MroXI GAANNNNTTC 2 cut(s) 2289, 2769
MseI TTAA 7 cut(s) 875, 1328, 1355, 1868, 1893, 2738, 3365
MslI CAYNNNNRTG 2 cut(s) 2269, 2722
MspA1I CMGCKG 4 cut(s) 11, 893, 2269, 2452
MspI CCGG 7 cut(s) 288, 332, 775, 1024, 1947, 2348, 3008
MspR9I CCNGG 6 cut(s) 155, 548, 1025, 1825, 2349, 3269
MunI CAATTG 1 cut(s) 1470
Mva1269I GAATGC 2 cut(s) 226, 585
MvaI CCWGG 4 cut(s) 155, 548, 1825, 3269
MvnI CGCG 1 cut(s) 3170
MwoI GCNNNNNNNGC 5 cut(s) 233, 2050, 2129, 2369, 2493
NciI CCSGG 2 cut(s) 1025, 2349
NcoI CCATGG 1 cut(s) 228
NlaIV GGNNCC 2 cut(s) 2033, 2182
NmuCI GTSAC 4 cut(s) 919, 2516, 2967, 3541
NsiI ATGCAT 3 cut(s) 133, 2475, 2614
NspI RCATGY 3 cut(s) 131, 1807, 3491
NspV TTCGAA 1 cut(s) 3040
OliI CACNNNNGTG 1 cut(s) 2269
PagI TCATGA 3 cut(s) 265, 948, 1498
PauI GCGCGC 1 cut(s) 3168
PceI AGGCCT 1 cut(s) 359
PciI ACATGT 2 cut(s) 1803, 3487
PciSI GCTCTTC 3 cut(s) 162, 1733, 2913
PcsI WCGNNNNNNNCGW 1 cut(s) 2526
PctI GAATGC 2 cut(s) 226, 585
PdmI GAANNNNTTC 2 cut(s) 2289, 2769
PfeI GAWTC 7 cut(s) 800, 2065, 2443, 3097, 3224, 3289, 3326
PflFI GACNNNGTC 1 cut(s) 338
PflMI CCANNNNNTGG 4 cut(s) 2191, 2438, 2910, 3527
PfoI TCCNGGA 1 cut(s) 2347
Ple19I CGATCG 1 cut(s) 433
PleI GAGTC 6 cut(s) 378, 467, 561, 763, 2167, 2974
PpsI GAGTC 6 cut(s) 378, 467, 561, 763, 2167, 2974
PpuMI RGGWCCY 1 cut(s) 2950
PscI ACATGT 2 cut(s) 1803, 3487
PshAI GACNNNNGTC 1 cut(s) 3548
PshBI ATTAAT 1 cut(s) 1328
PsiI TTATAA 1 cut(s) 2336
Psp5II RGGWCCY 1 cut(s) 2950
Psp6I CCWGG 4 cut(s) 153, 546, 1823, 3267
PspEI GGTNACC 1 cut(s) 1827
PspFI CCCAGC 1 cut(s) 1005
PspGI CCWGG 4 cut(s) 153, 546, 1823, 3267
PspN4I GGNNCC 2 cut(s) 2033, 2182
PspPI GGNCC 3 cut(s) 2950, 3509, 3530
PspPPI RGGWCCY 1 cut(s) 2950
PsrI GAACNNNNNNTAC 2 cut(s) 1837, 1869
PstI CTGCAG 2 cut(s) 726, 2307
PsuI RGATCY 5 cut(s) 241, 406, 771, 2180, 2832
PsyI GACNNNGTC 1 cut(s) 338
PteI GCGCGC 1 cut(s) 3168
PvuI CGATCG 1 cut(s) 433
PvuII CAGCTG 2 cut(s) 11, 2269
RsaI GTAC 6 cut(s) 956, 1403, 2413, 2503, 2524, 3372
RsaNI GTAC 6 cut(s) 955, 1402, 2412, 2502, 2523, 3371
RseI CAYNNNNRTG 2 cut(s) 2269, 2722
SapI GCTCTTC 3 cut(s) 162, 1733, 2913
SaqAI TTAA 7 cut(s) 875, 1328, 1355, 1868, 1893, 2738, 3365
Sau96I GGNCC 3 cut(s) 2950, 3509, 3530
ScaI AGTACT 1 cut(s) 1403
SchI GAGTC 6 cut(s) 379, 467, 562, 764, 2168, 2975
ScrFI CCNGG 6 cut(s) 155, 548, 1025, 1825, 2349, 3269
SduI GDGCHC 5 cut(s) 145, 2374, 3117, 3164, 3495
SfaNI GCATC 9 cut(s) 71, 151, 223, 896, 1192, 1827, 1959, 3418, 3596
SfcI CTRYAG 3 cut(s) 696, 722, 2303
SfuI TTCGAA 1 cut(s) 3040
SinI GGWCC 3 cut(s) 2950, 3509, 3530
SmiMI CAYNNNNRTG 2 cut(s) 2269, 2722
SmlI CTYRAG 6 cut(s) 144, 215, 2156, 2253, 2456, 3110
SmoI CTYRAG 6 cut(s) 144, 215, 2156, 2253, 2456, 3110
SseBI AGGCCT 1 cut(s) 359
SsiI CCGC 7 cut(s) 68, 115, 893, 928, 1648, 2450, 3377
SspMI CTAG 3 cut(s) 59, 1730, 2330
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 6 cut(s) 153, 546, 1023, 1823, 2347, 3267
StyI CCWWGG 3 cut(s) 228, 2437, 3215
TaaI ACNGT 9 cut(s) 393, 700, 2127, 2300, 2395, 2411, 2522, 2638, 2987
TaiI ACGT 2 cut(s) 2281, 2532
TatI WGTACW 3 cut(s) 954, 1401, 2411
TauI GCSGC 1 cut(s) 3379
TfiI GAWTC 7 cut(s) 800, 2065, 2443, 3097, 3224, 3289, 3326
Tru1I TTAA 7 cut(s) 875, 1328, 1355, 1868, 1893, 2738, 3365
Tru9I TTAA 7 cut(s) 875, 1328, 1355, 1868, 1893, 2738, 3365
TscAI CASTG 5 cut(s) 396, 1169, 1776, 2865, 2976
TseFI GTSAC 4 cut(s) 919, 2516, 2967, 3541
Tsp45I GTSAC 4 cut(s) 919, 2516, 2967, 3541
TspGWI ACGGA 1 cut(s) 863
TspRI CASTG 5 cut(s) 396, 1169, 1776, 2865, 2976
Tth111I GACNNNGTC 1 cut(s) 338
Van91I CCANNNNNTGG 4 cut(s) 2191, 2438, 2910, 3527
VpaK11BI GGWCC 3 cut(s) 2950, 3509, 3530
VspI ATTAAT 1 cut(s) 1328
XapI RAATTY 6 cut(s) 787, 1280, 1575, 1614, 2575, 2734
XceI RCATGY 3 cut(s) 131, 1807, 3491
XcmI CCANNNNNNNNNTGG 1 cut(s) 3503
XmiI GTMKAC 3 cut(s) 1105, 1716, 3451
XmnI GAANNNNTTC 2 cut(s) 2289, 2769
XspI CTAG 3 cut(s) 59, 1730, 2330
ZrmI AGTACT 1 cut(s) 1403
Zsp2I ATGCAT 3 cut(s) 133, 2475, 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.