RchiOBHm_Chr6g0269431

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000400
Physical Location & Seq
Forward (+)
26731413 .. 26734433
3021 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24170

Sequence Viewer

Length: 2631 bp
ATGACAGACTCTCCAAATTCCCCTTTTTATTTGAAGGGCTTAGAGGAGGAGGATTGCTGGGCTTTGTTCAAGCAACAGGCTTTTGGGCGAGGAGAAGAAGAGAAATATCCACACCTTTTACCAATTGGTAAGCAGATTGTCAGAAAATGTGGAGGTGTGCCATTAGCAGCAAAAAGTTTGGGAAGTATAATGCGCTTCAAAAGAAATCAACAACAGTGGTTGTCTGTGCAAAATACTGAACTTTGGAAATTGGATGTGTGCGAACTAGACATTCTTCCTGCCCTGATGTTGAGTTACTTTTATCTACCATTTCATCTTAGAAGATGCTTTGCCTTCTGCTCAATGTTTCCAAAAGGTTATGAATTCAGCAAGCACAAGTTAATTCACATGTGCATGGCGGAAGGCTTATTATTGGGGGAAGACGTGTGCAAGCGACCTGAAAATGTTGGTGATGACTACTTCACCAATTTGTTGTGGATGTCTTTCTTTCAAGAAGTAGATCGATGTGATGGTGGAGGTGTAATTGGGTACAAAATGAATGATGTCATTCATGACCTCTCACAGTATGTTGCAGGGGACACAACCGTGATACTTGAAAATGGTCTCCAACCAAAGAGTTTAGAAAATATTCGCCACTCCTCTGTTGTTTATAGATATAGAGCAATTACAATTCCACGAGCTCTGTATGAAGCACAACACCTGCGAAGTCTGTTATTTATTGGAGAATCTGGCTTGATAAAGAACATTCATAAAATCTGTTCAAGTTTTGTATACTTGCGTATGCTAGATCTGAACAGTTGTGATGTTCACAAATTGCCAGAATCATTGTGCGACTTAATATGTTTGAGATATCTTGACCTGTCTTACACACCTATATTCACTCTTCCTTATGACATGGCATGGATACTCGTGTCTCTGCAGACTTTAAACCTACTTGGTTGCCCTAATCTGAAGCGTTTGCCTTACTTGGGAGGCATGACAGGCCTAAGACATCTTGATGTAACTGCATGCAGAAGTTTGACTCGGATGCCTAAGGGTTTTGGAGAACTACATCAACTTGAGACATTGCCATTGTATGTTATTAGCTACGACGGCGAGGCCTTTGAGCTGGGTGGTTTGAATCTTTATGGCACGTTGAATCTCACACACTTGGAAAATGTAACCAAGGCAGTAGAAGCGAAATCAGCAGGATTGAGGATGAAAGAGAATATCGAGTCATTGGGACTACATTGGAAAGGCGAAGATGTGGATGAATCAATCATGATAAAAGTACCTAAAGCCCAACAAGAAGCAGTCATCTCAGTATCACAGACAGTGCCACAGAATTCTGACCCTGTGGAAGAAATTCTTGAAGCCTTGAAACCGCACGACAATTTGAAGATGCTAGTTATAAATGGGTATCCAGGAACTAGACTTCCTGATTGGACTCTCCCGAATCTAACTGCAGTTTATTTGAAGGAATGTAGAAATTGTCAGAATCTTCCAGCTCTTGGGAATCTCCTGATGCTTAAAACTCTTTCTTTGCAAGGAATGGATTGTCTGAAACACGTTGGTGCAGATTTCTATGGTGATGGTACCAGCATACCATTCTTGTCACTTGAAGAGTTATCTCTCTGTGATTTATCCAACATGGAAGAGTGGTCGAGTGCAAATAACATGACTTCATTCCCGAGATTGAGGAAGTTAACGGTTAACAGATGTCCCAAGTTAGCACATATACCATTCTCTGAATCTCTTCAACATTTGGAGTTGCGCGGTTGTAAACTACGTTTGATGTCCATAGCAAATTTAAGTTTACTTTCTGTGCTCGTCTTGGAAAATATTCCAGACCTATCCTCTCTCCCAGAAGGACTTATTGCATCAGCTCATCTGTCTTGTCTGAAGATTTTGTCATGCCCCAAGCTTTGTTCACTGCCTTTGGAGATGGGAAATTGCCTTACTAGTCTGAAATCATTGACCATTGGTTGGTGTCAAGATCTTTCGTCTCTGCCTGACAGTTTGCAAAACCTCAAAGCTCTGGAATCACTGGAGATTAGTGACTGTCACTACATAATCTCCATGCCGGATGGTGCTATTGGAGGTTTGAGTTCCCTTCGAACTTTGTCCATTGAGAACTGCACTAGTCTGACTTCCTTGTCCTCAAGTTTGGAACATTTGACATTCCTTGAACACTTGACCATTATGTATTGTCCAAGTCTTGATTCTTTTCCAGAGGGTGTGCAACACCTCTCTGCCCTTCGAAGTTTGACTATCCTGAGCTGTCCTTGGTTTGACTCTCTGCCAAATGGCTTACAAAATGTCAGGACACTACACTGTTTGGAAATTAGTAGCTGCACAAATCTAAGAGCTTTGCCAGAATGGTTTGAGAATCTTGACTCTCTTAGATCCTTGACCATATCTGCTTGTCCTAATTTAGAAGAATTGCCGGCAGGTCTAAAGCATCTCACTAAACTCCAGTACCTCTCTATCCAAGAATGTCCCGAGCTTGAGCAAAGATGCAGACAAGGAAGTGGAGAGGATTGGTTCAAAATAGCTCATGTTCCACATAAATACATTAGTTCTCCTCAGGTCTGTCAATCCAGCGAAGCAAGCACCTCAGGCGGATCGTCTTCTCAAACATCTGCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

876

Amino Acids

98.02

Weight (kDa)

5.74

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 115 - 186 7.3e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 233 - 565 5.2e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 369 - 511 1e-28 R13L1/DRL21 LRRs
LRR_13 PF23286 646 - 832 2.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 696 - 825 2.1e-12 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 763 - 826 3.4e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1391
AarI CACCTGC 1 cut(s) 708
AasI GACNNNNNNGTC 1 cut(s) 2134
Acc36I ACCTGC 2 cut(s) 708, 2420
Acc65I GGTACC 1 cut(s) 1574
AccB1I GGYRCC 1 cut(s) 1574
AccB7I CCANNNNNTGG 2 cut(s) 1490, 1965
AccI GTMKAC 1 cut(s) 771
AccII CGCG 1 cut(s) 1755
AciI CCGC 4 cut(s) 398, 1364, 1755, 2601
AclWI GGATC 2 cut(s) 2379, 2611
AcsI RAATTY 5 cut(s) 16, 362, 1324, 1344, 1786
AcuI CTGAAG 2 cut(s) 971, 1901
AfaI GTAC 4 cut(s) 530, 1272, 1576, 2459
AfiI CCNNNNNNNGG 3 cut(s) 968, 1490, 1965
AflIII ACRYGT 3 cut(s) 387, 423, 1546
AhlI ACTAGT 2 cut(s) 1940, 2120
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 2185, 2217
AleI CACNNNNGTG 2 cut(s) 584, 1551
AloI GAACNNNNNNTCC 2 cut(s) 1399, 1431
Alw21I GWGCWC 2 cut(s) 682, 1809
Alw26I GTCTC 4 cut(s) 608, 918, 1055, 1989
AlwI GGATC 2 cut(s) 2379, 2611
Ama87I CYCGRG 2 cut(s) 1669, 2480
AoxI GGCC 2 cut(s) 982, 1098
ApeKI GCWGC 2 cut(s) 167, 2331
ApoI RAATTY 5 cut(s) 16, 362, 1324, 1344, 1786
Asp700I GAANNNNTTC 4 cut(s) 627, 1344, 1734, 1821
Asp718I GGTACC 1 cut(s) 1574
AspLEI GCGC 2 cut(s) 195, 1755
AsuHPI GGTGA 3 cut(s) 454, 461, 1580
AsuII TTCGAA 2 cut(s) 2095, 2239
AvaI CYCGRG 2 cut(s) 1669, 2480
AxyI CCTNAGG 3 cut(s) 1032, 2565, 2596
BaeI ACNNNNGTAYC 4 cut(s) 896, 929, 2441, 2474
BanI GGYRCC 1 cut(s) 1574
BanII GRGCYC 1 cut(s) 682
BauI CACGAG 2 cut(s) 675, 908
BbsI GAAGAC 2 cut(s) 426, 2601
Bbv12I GWGCWC 2 cut(s) 682, 1809
BbvI GCAGC 2 cut(s) 179, 2318
BccI CCATC 4 cut(s) 503, 1565, 1918, 2060
BceAI ACGGC 1 cut(s) 1108
BciT130I CCWGG 1 cut(s) 1404
BciVI GTATCC 2 cut(s) 897, 1410
BcoDI GTCTC 4 cut(s) 608, 918, 1055, 1989
BcuI ACTAGT 2 cut(s) 1940, 2120
BfaI CTAG 6 cut(s) 266, 785, 1385, 1410, 1941, 2121
BfmI CTRYAG 2 cut(s) 917, 1443
BfuAI ACCTGC 2 cut(s) 708, 2420
BfuI GTATCC 2 cut(s) 897, 1410
BglII AGATCT 2 cut(s) 787, 1975
BisI GCNGC 2 cut(s) 168, 2332
BlsI GCNGC 2 cut(s) 169, 2333
Bme1390I CCNGG 1 cut(s) 1404
BmeT110I CYCGRG 2 cut(s) 1669, 2480
BmgBI CACGTC 1 cut(s) 424
BmiI GGNNCC 1 cut(s) 1576
BmrFI CCNGG 1 cut(s) 1404
BmsI GCATC 7 cut(s) 314, 1017, 1371, 1494, 1868, 2449, 2486
BpiI GAAGAC 2 cut(s) 426, 2601
BpmI CTGGAG 2 cut(s) 2048, 2438
Bpu10I CCTNAGC 1 cut(s) 2255
Bpu14I TTCGAA 2 cut(s) 2095, 2239
BpuEI CTTGAG 3 cut(s) 1079, 2125, 2507
Bsa29I ATCGAT 1 cut(s) 502
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 2 cut(s) 1164, 2264
BsaXI ACNNNNNCTCC 3 cut(s) 25, 2039, 2069
Bsc4I CCNNNNNNNGG 3 cut(s) 968, 1490, 1965
Bse118I RCCGGY 1 cut(s) 2425
Bse1I ACTGG 2 cut(s) 2031, 2455
Bse21I CCTNAGG 3 cut(s) 1032, 2565, 2596
Bse3DI GCAATG 1 cut(s) 1064
BseBI CCWGG 1 cut(s) 1404
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 2 cut(s) 1164, 2264
BseGI GGATG 6 cut(s) 259, 483, 1032, 1203, 1255, 2071
BseLI CCNNNNNNNGG 3 cut(s) 968, 1490, 1965
BseMI GCAATG 1 cut(s) 1064
BseMII CTCAG 4 cut(s) 1314, 2246, 2579, 2610
BseNI ACTGG 2 cut(s) 2031, 2455
BseRI GAGGAG 5 cut(s) 59, 62, 105, 628, 2553
BseXI GCAGC 2 cut(s) 179, 2318
BseYI CCCAGC 2 cut(s) 57, 1108
BsgI GTGCAG 3 cut(s) 1575, 2101, 2317
Bsh1236I CGCG 1 cut(s) 1755
BshFI GGCC 2 cut(s) 984, 1100
BshNI GGYRCC 1 cut(s) 1574
BshVI ATCGAT 1 cut(s) 502
BsiHKAI GWGCWC 2 cut(s) 682, 1809
BsiHKCI CYCGRG 2 cut(s) 1669, 2480
BsiSI CCGG 2 cut(s) 2063, 2426
BslFI GGGAC 4 cut(s) 590, 1236, 1686, 2463
BslI CCNNNNNNNGG 3 cut(s) 968, 1490, 1965
BsmAI GTCTC 4 cut(s) 608, 918, 1055, 1989
BsmBI CGTCTC 1 cut(s) 1989
BsmFI GGGAC 4 cut(s) 590, 1236, 1686, 2463
BsnI GGCC 2 cut(s) 984, 1100
Bso31I GGTCTC 1 cut(s) 608
BsoBI CYCGRG 2 cut(s) 1669, 2480
Bsp119I TTCGAA 2 cut(s) 2095, 2239
Bsp1286I GDGCHC 2 cut(s) 682, 1809
Bsp143I GATC 5 cut(s) 499, 787, 1975, 2384, 2603
BspACI CCGC 4 cut(s) 398, 1364, 1755, 2601
BspANI GGCC 2 cut(s) 984, 1100
BspCNI CTCAG 4 cut(s) 1313, 2247, 2578, 2609
BspDI ATCGAT 1 cut(s) 502
BspFNI CGCG 1 cut(s) 1755
BspHI TCATGA 2 cut(s) 550, 1260
BspLI GGNNCC 1 cut(s) 1576
BspMAI CTGCAG 2 cut(s) 921, 1447
BspMI ACCTGC 2 cut(s) 708, 2420
BspPI GGATC 2 cut(s) 2379, 2611
BspT104I TTCGAA 2 cut(s) 2095, 2239
BspT107I GGYRCC 1 cut(s) 1574
BspTNI GGTCTC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 1064
BsrFI RCCGGY 1 cut(s) 2425
BsrI ACTGG 2 cut(s) 2031, 2455
BssAI RCCGGY 1 cut(s) 2425
BssECI CCNNGG 2 cut(s) 1164, 2264
BssMI GATC 5 cut(s) 499, 787, 1975, 2384, 2603
BssNAI GTATAC 1 cut(s) 772
BssSI CACGAG 2 cut(s) 675, 908
BssT1I CCWWGG 2 cut(s) 1164, 2264
Bst1107I GTATAC 1 cut(s) 772
Bst2BI CACGAG 2 cut(s) 675, 908
Bst2UI CCWGG 1 cut(s) 1404
Bst4CI ACNGT 9 cut(s) 216, 564, 586, 797, 1315, 1690, 1997, 2042, 2315
Bst6I CTCTTC 5 cut(s) 93, 888, 1596, 1629, 1740
BstBI TTCGAA 2 cut(s) 2095, 2239
BstC8I GCNNGC 5 cut(s) 371, 431, 1009, 2427, 2590
BstF5I GGATG 6 cut(s) 259, 483, 1032, 1203, 1255, 2071
BstFNI CGCG 1 cut(s) 1755
BstHHI GCGC 2 cut(s) 195, 1755
BstKTI GATC 5 cut(s) 502, 790, 1978, 2387, 2606
BstMAI GTCTC 4 cut(s) 608, 918, 1055, 1989
BstMBI GATC 5 cut(s) 499, 787, 1975, 2384, 2603
BstMWI GCNNNNNNNGC 6 cut(s) 981, 1092, 1175, 1184, 2589, 2598
BstNI CCWGG 1 cut(s) 1404
BstNSI RCATGY 2 cut(s) 391, 1011
BstSCI CCNGG 1 cut(s) 1402
BstSFI CTRYAG 2 cut(s) 917, 1443
BstUI CGCG 1 cut(s) 1755
BstV1I GCAGC 2 cut(s) 179, 2318
BstV2I GAAGAC 2 cut(s) 426, 2601
BstX2I RGATCY 3 cut(s) 787, 1975, 2384
BstYI RGATCY 3 cut(s) 787, 1975, 2384
BstZ17I GTATAC 1 cut(s) 772
Bsu15I ATCGAT 1 cut(s) 502
Bsu36I CCTNAGG 3 cut(s) 1032, 2565, 2596
BsuI GTATCC 2 cut(s) 897, 1410
BsuRI GGCC 2 cut(s) 984, 1100
BsuTUI ATCGAT 1 cut(s) 502
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 6 cut(s) 259, 483, 1032, 1203, 1255, 2071
BtsI GCAGTG 1 cut(s) 1910
BtsIMutI CAGTG 5 cut(s) 221, 1320, 1910, 2024, 2311
BveI ACCTGC 2 cut(s) 708, 2420
Cac8I GCNNGC 5 cut(s) 371, 431, 1009, 2427, 2590
CciI TCATGA 2 cut(s) 550, 1260
CfoI GCGC 2 cut(s) 195, 1755
Cfr10I RCCGGY 1 cut(s) 2425
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 4 cut(s) 529, 1271, 1575, 2458
CspCI CAANNNNNGTGG 2 cut(s) 197, 232
CviQI GTAC 4 cut(s) 529, 1271, 1575, 2458
DpnI GATC 5 cut(s) 501, 789, 1977, 2386, 2605
DpnII GATC 5 cut(s) 499, 787, 1975, 2384, 2603
DraI TTTAAA 1 cut(s) 927
DrdI GACNNNNNNGTC 1 cut(s) 2134
DseDI GACNNNNNNGTC 1 cut(s) 2134
Eam1104I CTCTTC 5 cut(s) 93, 888, 1596, 1629, 1740
EarI CTCTTC 5 cut(s) 93, 888, 1596, 1629, 1740
EciI GGCGGA 2 cut(s) 413, 2616
Ecl136II GAGCTC 1 cut(s) 680
Eco130I CCWWGG 2 cut(s) 1164, 2264
Eco147I AGGCCT 2 cut(s) 984, 1100
Eco24I GRGCYC 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 608
Eco32I GATATC 1 cut(s) 851
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 2 cut(s) 971, 1901
Eco81I CCTNAGG 3 cut(s) 1032, 2565, 2596
Eco88I CYCGRG 2 cut(s) 1669, 2480
EcoICRI GAGCTC 1 cut(s) 680
EcoRI GAATTC 2 cut(s) 362, 1324
EcoRII CCWGG 1 cut(s) 1402
EcoRV GATATC 1 cut(s) 851
EcoT14I CCWWGG 2 cut(s) 1164, 2264
EcoT38I GRGCYC 1 cut(s) 682
ErhI CCWWGG 2 cut(s) 1164, 2264
Esp3I CGTCTC 1 cut(s) 1989
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FaqI GGGAC 4 cut(s) 590, 1236, 1686, 2463
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 2 cut(s) 168, 2332
FokI GGATG 6 cut(s) 266, 490, 1039, 1210, 1262, 2078
FriOI GRGCYC 1 cut(s) 682
Fsp4HI GCNGC 2 cut(s) 168, 2332
FspBI CTAG 6 cut(s) 266, 785, 1385, 1410, 1941, 2121
GlaI GCGC 2 cut(s) 194, 1754
GluI GCNGC 2 cut(s) 168, 2332
GsaI CCCAGC 2 cut(s) 61, 1112
GsuI CTGGAG 2 cut(s) 2048, 2438
HaeIII GGCC 2 cut(s) 984, 1100
HapII CCGG 2 cut(s) 2063, 2426
HhaI GCGC 2 cut(s) 195, 1755
Hin6I GCGC 2 cut(s) 193, 1753
HinP1I GCGC 2 cut(s) 193, 1753
HincII GTYRAC 2 cut(s) 1686, 1693
HindII GTYRAC 2 cut(s) 1686, 1693
HindIII AAGCTT 1 cut(s) 1901
HpaI GTTAAC 2 cut(s) 1686, 1693
HpaII CCGG 2 cut(s) 2063, 2426
HphI GGTGA 3 cut(s) 454, 461, 1580
Hpy166II GTNNAC 7 cut(s) 772, 808, 1686, 1693, 1763, 1796, 1910
Hpy8I GTNNAC 7 cut(s) 772, 808, 1686, 1693, 1763, 1796, 1910
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 7 cut(s) 28, 343, 395, 1450, 1841, 2102, 2246
HpyCH4III ACNGT 9 cut(s) 216, 564, 586, 797, 1315, 1690, 1997, 2042, 2315
HpyCH4IV ACGT 4 cut(s) 423, 1133, 1548, 1768
HpyF10VI GCNNNNNNNGC 6 cut(s) 981, 1092, 1175, 1184, 2589, 2598
HpySE526I ACGT 4 cut(s) 423, 1133, 1548, 1768
HspAI GCGC 2 cut(s) 193, 1753
KpnI GGTACC 1 cut(s) 1578
KroI GCCGGC 1 cut(s) 2425
KroNI GCCGGC 1 cut(s) 2427
KspAI GTTAAC 2 cut(s) 1686, 1693
Kzo9I GATC 5 cut(s) 499, 787, 1975, 2384, 2603
Lsp1109I GCAGC 2 cut(s) 179, 2318
LweI GCATC 7 cut(s) 314, 1017, 1371, 1494, 1868, 2449, 2486
MaeI CTAG 6 cut(s) 266, 785, 1385, 1410, 1941, 2121
MaeII ACGT 4 cut(s) 423, 1133, 1548, 1768
MaeIII GTNAC 6 cut(s) 293, 1000, 1159, 1593, 2036, 2042
MalI GATC 5 cut(s) 501, 789, 1977, 2386, 2605
MboI GATC 5 cut(s) 499, 787, 1975, 2384, 2603
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 3 cut(s) 787, 1975, 2384
MhlI GDGCHC 2 cut(s) 682, 1809
MlyI GAGTC 6 cut(s) 2, 1015, 1223, 1420, 2267, 2369
MmeI TCCRAC 2 cut(s) 631, 1650
MroNI GCCGGC 1 cut(s) 2425
MroXI GAANNNNTTC 4 cut(s) 627, 1344, 1734, 1821
MseI TTAA 7 cut(s) 380, 836, 926, 1509, 1685, 1692, 1790
MslI CAYNNNNRTG 4 cut(s) 392, 584, 996, 1551
MspI CCGG 2 cut(s) 2063, 2426
MspR9I CCNGG 1 cut(s) 1404
MunI CAATTG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 1404
MvnI CGCG 1 cut(s) 1755
MwoI GCNNNNNNNGC 6 cut(s) 981, 1092, 1175, 1184, 2589, 2598
NaeI GCCGGC 1 cut(s) 2427
NdeII GATC 5 cut(s) 499, 787, 1975, 2384, 2603
NgoMIV GCCGGC 1 cut(s) 2425
NlaIV GGNNCC 1 cut(s) 1576
NmuCI GTSAC 3 cut(s) 1593, 2036, 2042
NspI RCATGY 2 cut(s) 391, 1011
NspV TTCGAA 2 cut(s) 2095, 2239
OliI CACNNNNGTG 2 cut(s) 584, 1551
PaeI GCATGC 1 cut(s) 1011
PagI TCATGA 2 cut(s) 550, 1260
PaqCI CACCTGC 1 cut(s) 708
PceI AGGCCT 2 cut(s) 984, 1100
PciI ACATGT 1 cut(s) 387
PdiI GCCGGC 1 cut(s) 2427
PdmI GAANNNNTTC 4 cut(s) 627, 1344, 1734, 1821
PflMI CCANNNNNTGG 2 cut(s) 1490, 1965
PfoI TCCNGGA 1 cut(s) 1402
PkrI GCNGC 2 cut(s) 169, 2333
PleI GAGTC 6 cut(s) 2, 1015, 1222, 1420, 2267, 2369
PpsI GAGTC 6 cut(s) 2, 1015, 1222, 1420, 2267, 2369
PscI ACATGT 1 cut(s) 387
PsiI TTATAA 1 cut(s) 1391
Psp124BI GAGCTC 1 cut(s) 682
Psp6I CCWGG 1 cut(s) 1402
PspFI CCCAGC 2 cut(s) 57, 1108
PspGI CCWGG 1 cut(s) 1402
PspN4I GGNNCC 1 cut(s) 1576
PstI CTGCAG 2 cut(s) 921, 1447
PsuI RGATCY 3 cut(s) 787, 1975, 2384
RsaI GTAC 4 cut(s) 530, 1272, 1576, 2459
RsaNI GTAC 4 cut(s) 529, 1271, 1575, 2458
RseI CAYNNNNRTG 4 cut(s) 392, 584, 996, 1551
SacI GAGCTC 1 cut(s) 682
SaqAI TTAA 7 cut(s) 380, 836, 926, 1509, 1685, 1692, 1790
SatI GCNGC 2 cut(s) 168, 2332
Sau3AI GATC 5 cut(s) 499, 787, 1975, 2384, 2603
SchI GAGTC 6 cut(s) 2, 1015, 1223, 1420, 2267, 2369
ScrFI CCNGG 1 cut(s) 1404
SduI GDGCHC 2 cut(s) 682, 1809
SfaNI GCATC 7 cut(s) 314, 1017, 1371, 1494, 1868, 2449, 2486
SfcI CTRYAG 2 cut(s) 917, 1443
SfuI TTCGAA 2 cut(s) 2095, 2239
SmiMI CAYNNNNRTG 4 cut(s) 392, 584, 996, 1551
SmlI CTYRAG 3 cut(s) 1058, 2140, 2486
SmoI CTYRAG 3 cut(s) 1058, 2140, 2486
SpeI ACTAGT 2 cut(s) 1940, 2120
SphI GCATGC 1 cut(s) 1011
SseBI AGGCCT 2 cut(s) 984, 1100
SsiI CCGC 4 cut(s) 398, 1364, 1755, 2601
SspI AATATT 2 cut(s) 628, 1822
SspMI CTAG 6 cut(s) 266, 785, 1385, 1410, 1941, 2121
SstI GAGCTC 1 cut(s) 682
StuI AGGCCT 2 cut(s) 984, 1100
StyD4I CCNGG 1 cut(s) 1402
StyI CCWWGG 2 cut(s) 1164, 2264
TaaI ACNGT 9 cut(s) 216, 564, 586, 797, 1315, 1690, 1997, 2042, 2315
TaiI ACGT 4 cut(s) 426, 1136, 1551, 1771
TaqI TCGA 5 cut(s) 502, 1212, 1643, 2095, 2239
Tru1I TTAA 7 cut(s) 380, 836, 926, 1509, 1685, 1692, 1790
Tru9I TTAA 7 cut(s) 380, 836, 926, 1509, 1685, 1692, 1790
TscAI CASTG 5 cut(s) 221, 1320, 1917, 2031, 2318
TseFI GTSAC 3 cut(s) 1593, 2036, 2042
TseI GCWGC 2 cut(s) 167, 2331
Tsp45I GTSAC 3 cut(s) 1593, 2036, 2042
TspDTI ATGAA 9 cut(s) 302, 375, 539, 551, 702, 737, 1214, 1266, 1653
TspRI CASTG 5 cut(s) 221, 1320, 1917, 2031, 2318
Van91I CCANNNNNTGG 2 cut(s) 1490, 1965
XapI RAATTY 5 cut(s) 16, 362, 1324, 1344, 1786
XceI RCATGY 2 cut(s) 391, 1011
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 4 cut(s) 627, 1344, 1734, 1821
XspI CTAG 6 cut(s) 266, 785, 1385, 1410, 1941, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.