Rh1AG213000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000400
Physical Location & Seq
Reverse (-)
40698864 .. 40702913
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG213000.1

Sequence Viewer

Length: 3570 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCGGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTCGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGACTTCGTTGCTGCTAAAGACATCATTATGGGCCCTGGAAAATTATGTAAGTGGACCTATCCAATTCCAAAATACATAGATGCTGATAAAGTCAGAAAGATGCTTTTGGCTTTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTTCAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTTCTGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTAGTGAAACTGTTACTATCTTCTGAGTGTCACCAGGATGGATATGCTATGTGTATTGACTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCCCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATATTAGGCTGTGGACTTTTGTCTCGCATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACGAGGGAAAATTGCAAGTTCTCGGAGAGTGACTTACTTCAAGCTCGGCTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGAGGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAGTCCCATTCATGATGGACTCCCCAACTTCGGCATATTATTTGAAGGGTTTGATGGAGGATGACTGCTGGGCTTTGTTCAAGCAACGAGCTTTTGGACGAGGAGAAGAAGAGAAGTATGCCAACCTCTGCCTGATTGGAAAGAAGATTGCGAAGAAATGTGGTGGTGTACCATTGGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTGTGGGAAGTAGAAGGATGGAAACAAAGAATTTTACCTGCGCTTATGTTGAGTTATCTTCATCTGCCATCACATCTTCGACAATGCTTTGCGTTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAAGTAGCAAGCGACCGGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGCAGCACAGTTGGATACAAAATGAATGATGTCATTCACGACCTTGCACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCGAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGCACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAGTTGTATATACTTGCGTGTGCTGGACTTGAACAACTGTGATGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACACCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCCAGACTTCGAAGTAATGGCCGGCCTAAGACATCTCAATATAGCTGGATGTGATAGGTTGTGGTATATACCTCCTGGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCAAAAGAGGGTCGGTGATCTTGGTGTACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATAACACGCTTGGAGAATGTGAGAAGCGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATCTTGAGTCGTTGGGATTATGTTGGGGAGATTTTGAAGATGTGAACGACAGGAAAAAATCATTGAAGAAACCAGCCAGGTCCTCAGCCAAGTCCTCAGCCAAATGGTACTGTTCAGAAGTAGGGATGCGGTGGCATGAACCTGATGCTGAAGAGGCGGTAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAGTCAGATTTCCAGATTGGACTCTCCCAAATGTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCTTCTACTCAAGACTCTTTCTCTGCACGGAATGGAGAGCGTGACACGCATTGGTTGTGAGTTCTATGGTGATAGTACAGACATATGCTTTTCATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAACTTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATAAATAGGTGTCCCAAGTTGACACATATCCCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCATTGGAGGTTTAACATTGCTGACAATGCTTGTAATAGAAAAACTTCCAGAGCTGCGCTCTCTACCACCAGGGCTTTCTGCATCAGCTAATCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCCCTGCCTTTGGAGATCGGAAACCTCACTGCGCTGAAATCATTAACCATTCGTTGGTGTGATGAGTTGTCCTCTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAGTCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGAGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAATCTGACGTCTTTGTCATCGAGTTTGGAACAACTTGCTTTCCTTGAGCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCGCTGAGGGTGTGCAACACCTCTCAACCCTTCGAAGTTTGTCTATATTGAGCTGTCCTTGGTTTGATTCTTTGCCTGAAGGGTTGCAAATGGTCAAGACGTTGCAGTGTCTTGAAATTAGTAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATTTTTGATTGTGCTAATTTGAAACTGTTGCCACAAAGTCTAAAGCTTCTCACTAAACTTCAGCACCTCTCTATTCAAGAATGTCCTGAGCTTGAGAGAAGATGTAGACAAGGAAGCGGTGAGGAGTGGCTTAAAATAGCTCATGTTCCACATAAGTACATTGGATCACCAGAGATCAGGCAGTCTGGTAGTGGAGCAAGCACATCCAGCAGCTCTTCTTTTGATACATCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1189

Amino Acids

133.94

Weight (kDa)

6.12

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 84 3.6e-17 Rx N-terminal domain
NB-ARC PF00931 177 - 335 5e-36 NB-ARC domain
WHD_DRP PF23559 421 - 492 1.4e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 556 - 896 1.2e-19 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 673 - 825 1.1e-26 R13L1/DRL21 LRRs
LRR_14 PF23598 869 - 991 1.7e-06 Leucine-rich repeat region
LRR_14 PF23598 956 - 1137 1.1e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1566
AasI GACNNNNNNGTC 1 cut(s) 3073
AatII GACGTC 1 cut(s) 3071
Acc36I ACCTGC 1 cut(s) 1204
AccB7I CCANNNNNTGG 3 cut(s) 2432, 2904, 3137
AccBSI CCGCTC 3 cut(s) 622, 1551, 3109
AccI GTMKAC 1 cut(s) 3439
AciI CCGC 9 cut(s) 77, 622, 898, 1549, 2251, 2279, 2695, 3109, 3450
AclWI GGATC 1 cut(s) 3505
AcoI YGGCCR 1 cut(s) 1890
AcsI RAATTY 7 cut(s) 1188, 1280, 1542, 2029, 2286, 2314, 3277
AcuI CTGAAG 6 cut(s) 840, 1955, 2129, 2292, 3243, 3377
AcyI GRCGYC 1 cut(s) 3068
AfaI GTAC 6 cut(s) 1077, 2022, 2231, 2518, 3125, 3491
AfiI CCNNNNNNNGG 7 cut(s) 566, 937, 2432, 2637, 2860, 2904, 3137
AjnI CCWGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
AjuI GAANNNNNNNTTGG 2 cut(s) 3124, 3156
AloI GAACNNNNNNTCC 2 cut(s) 1807, 1839
Alw21I GWGCWC 1 cut(s) 2095
Alw26I GTCTC 3 cut(s) 428, 667, 2679
AlwI GGATC 1 cut(s) 3505
AlwNI CAGNNNCTG 1 cut(s) 1978
AoxI GGCC 4 cut(s) 271, 1890, 1894, 2296
ApaI GGGCCC 1 cut(s) 275
ApoI RAATTY 7 cut(s) 1188, 1280, 1542, 2029, 2286, 2314, 3277
Asp700I GAANNNNTTC 2 cut(s) 2421, 2763
AspLEI GCGC 5 cut(s) 1113, 1201, 2084, 2778, 2884
AspS9I GGNCC 4 cut(s) 271, 272, 294, 2202
AsuHPI GGTGA 8 cut(s) 12, 515, 1376, 2021, 2522, 3106, 3464, 3492
AsuII TTCGAA 2 cut(s) 1881, 3178
AvaII GGWCC 2 cut(s) 294, 2202
BaeGI GKGCMC 1 cut(s) 275
BaeI ACNNNNGTAYC 1 cut(s) 3549
BanII GRGCYC 2 cut(s) 275, 2095
BarI GAAGNNNNNNTAC 4 cut(s) 1401, 1433, 2695, 2727
BbsI GAAGAC 3 cut(s) 1362, 1511, 2808
Bbv12I GWGCWC 1 cut(s) 2095
BbvCI CCTCAGC 3 cut(s) 2206, 2218, 3150
BceAI ACGGC 3 cut(s) 167, 1145, 1717
BciT130I CCWGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
BciVI GTATCC 1 cut(s) 1436
BcoDI GTCTC 3 cut(s) 428, 667, 2679
BfaI CTAG 5 cut(s) 438, 1331, 2024, 2327, 2966
BfmI CTRYAG 1 cut(s) 3055
BfuAI ACCTGC 1 cut(s) 1204
BfuI GTATCC 1 cut(s) 1436
BglI GCCNNNNNGGC 1 cut(s) 605
BglII AGATCT 1 cut(s) 3323
Bme1390I CCNGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
Bme18I GGWCC 2 cut(s) 294, 2202
BmgT120I GGNCC 4 cut(s) 271, 272, 294, 2202
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
BmrI ACTGGG 3 cut(s) 53, 595, 844
BmuI ACTGGG 3 cut(s) 53, 595, 844
BoxI GACNNNNGTC 1 cut(s) 659
BpiI GAAGAC 3 cut(s) 1362, 1511, 2808
BplI GAGNNNNNCTC 6 cut(s) 425, 457, 2762, 2794, 2906, 2938
BpmI CTGGAG 4 cut(s) 89, 2109, 2843, 2978
Bpu10I CCTNAGC 4 cut(s) 2206, 2218, 3150, 3420
Bpu14I TTCGAA 2 cut(s) 1881, 3178
BpuEI CTTGAG 6 cut(s) 191, 2147, 2435, 2638, 3125, 3446
BsaAI YACGTR 1 cut(s) 1421
BsaBI GATNNNNATC 1 cut(s) 2703
BsaHI GRCGYC 1 cut(s) 3068
BsaJI CCNNGG 6 cut(s) 123, 274, 2431, 2789, 3136, 3203
BsaWI WCCGGW 1 cut(s) 1351
Bsc4I CCNNNNNNNGG 7 cut(s) 566, 937, 2432, 2637, 2860, 2904, 3137
Bse118I RCCGGY 2 cut(s) 1801, 1892
Bse1I ACTGG 4 cut(s) 59, 601, 839, 2092
Bse3DI GCAATG 3 cut(s) 1139, 1826, 2735
Bse8I GATNNNNATC 1 cut(s) 2703
BseBI CCWGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
BseDI CCNNGG 6 cut(s) 123, 274, 2431, 2789, 3136, 3203
BseJI GATNNNNATC 1 cut(s) 2703
BseLI CCNNNNNNNGG 7 cut(s) 566, 937, 2432, 2637, 2860, 2904, 3137
BseMI GCAATG 3 cut(s) 1139, 1826, 2735
BseMII CTCAG 8 cut(s) 444, 507, 2220, 2232, 2569, 3141, 3334, 3411
BseNI ACTGG 4 cut(s) 59, 601, 839, 2092
BseRI GAGGAG 4 cut(s) 173, 876, 1023, 3470
BseSI GKGCMC 1 cut(s) 275
BseYI CCCAGC 1 cut(s) 975
BsgI GTGCAG 2 cut(s) 761, 2450
Bsh1285I CGRYCG 1 cut(s) 1352
BshFI GGCC 4 cut(s) 273, 1892, 1896, 2298
BsiEI CGRYCG 1 cut(s) 1352
BsiHKAI GWGCWC 1 cut(s) 2095
BsiSI CCGG 4 cut(s) 187, 1352, 1802, 1893
BslFI GGGAC 4 cut(s) 101, 851, 896, 2628
BslI CCNNNNNNNGG 7 cut(s) 566, 937, 2432, 2637, 2860, 2904, 3137
BsmAI GTCTC 3 cut(s) 428, 667, 2679
BsmFI GGGAC 4 cut(s) 101, 851, 896, 2628
BsnI GGCC 4 cut(s) 273, 1892, 1896, 2298
Bsp119I TTCGAA 2 cut(s) 1881, 3178
Bsp120I GGGCCC 1 cut(s) 271
Bsp1286I GDGCHC 2 cut(s) 275, 2095
Bsp143I GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
BspACI CCGC 9 cut(s) 77, 622, 898, 1549, 2251, 2279, 2695, 3109, 3450
BspANI GGCC 4 cut(s) 273, 1892, 1896, 2298
BspCNI CTCAG 8 cut(s) 445, 508, 2219, 2231, 2568, 3142, 3333, 3412
BspHI TCATGA 3 cut(s) 220, 690, 918
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 3059
BspMI ACCTGC 1 cut(s) 1204
BspPI GGATC 1 cut(s) 3505
BspQI GCTCTTC 2 cut(s) 466, 3553
BspT104I TTCGAA 2 cut(s) 1881, 3178
BsrBI CCGCTC 3 cut(s) 622, 1551, 3109
BsrDI GCAATG 3 cut(s) 1139, 1826, 2735
BsrFI RCCGGY 2 cut(s) 1801, 1892
BsrI ACTGG 4 cut(s) 59, 601, 839, 2092
BssAI RCCGGY 2 cut(s) 1801, 1892
BssECI CCNNGG 6 cut(s) 123, 274, 2431, 2789, 3136, 3203
BssMI GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
BssNI GRCGYC 1 cut(s) 3068
BssT1I CCWWGG 4 cut(s) 123, 2431, 3136, 3203
Bst2UI CCWGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
Bst6I CTCTTC 5 cut(s) 466, 1011, 2268, 2682, 3553
BstACI GRCGYC 1 cut(s) 3068
BstAPI GCANNNNNTGC 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 1421
BstBI TTCGAA 2 cut(s) 1881, 3178
BstC8I GCNNGC 6 cut(s) 75, 1346, 1803, 1894, 3312, 3532
BstHHI GCGC 5 cut(s) 1113, 1201, 2084, 2778, 2884
BstKTI GATC 8 cut(s) 39, 690, 826, 2014, 2868, 3326, 3500, 3510
BstMAI GTCTC 3 cut(s) 428, 667, 2679
BstMBI GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
BstMCI CGRYCG 1 cut(s) 1352
BstMWI GCNNNNNNNGC 9 cut(s) 202, 224, 605, 775, 2090, 2276, 2487, 2746, 3540
BstNI CCWGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 659
BstSCI CCNGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
BstSFI CTRYAG 1 cut(s) 3055
BstSLI GKGCMC 1 cut(s) 275
BstV2I GAAGAC 3 cut(s) 1362, 1511, 2808
BstX2I RGATCY 1 cut(s) 3323
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 3323
BsuI GTATCC 1 cut(s) 1436
BsuRI GGCC 4 cut(s) 273, 1892, 1896, 2298
BtsI GCAGTG 3 cut(s) 1976, 2877, 3257
BtsIMutI CAGTG 4 cut(s) 1976, 2563, 2877, 3257
BveI ACCTGC 1 cut(s) 1204
Cac8I GCNNGC 6 cut(s) 75, 1346, 1803, 1894, 3312, 3532
CaiI CAGNNNCTG 1 cut(s) 1978
CciI TCATGA 3 cut(s) 220, 690, 918
CfoI GCGC 5 cut(s) 1113, 1201, 2084, 2778, 2884
Cfr10I RCCGGY 2 cut(s) 1801, 1892
Cfr13I GGNCC 4 cut(s) 271, 272, 294, 2202
CsiI ACCWGGT 1 cut(s) 2579
Csp6I GTAC 6 cut(s) 1076, 2021, 2230, 2517, 3124, 3490
CspCI CAANNNNNGTGG 2 cut(s) 2919, 2954
CviQI GTAC 6 cut(s) 1076, 2021, 2230, 2517, 3124, 3490
DpnI GATC 8 cut(s) 38, 689, 825, 2013, 2867, 3325, 3499, 3509
DpnII GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
DraI TTTAAA 2 cut(s) 1117, 1839
DrdI GACNNNNNNGTC 1 cut(s) 3073
DseDI GACNNNNNNGTC 1 cut(s) 3073
EaeI YGGCCR 1 cut(s) 1890
Eam1104I CTCTTC 5 cut(s) 466, 1011, 2268, 2682, 3553
EarI CTCTTC 5 cut(s) 466, 1011, 2268, 2682, 3553
Ecl136II GAGCTC 1 cut(s) 2093
Eco130I CCWWGG 4 cut(s) 123, 2431, 3136, 3203
Eco24I GRGCYC 2 cut(s) 275, 2095
Eco32I GATATC 2 cut(s) 82, 791
Eco47I GGWCC 2 cut(s) 294, 2202
Eco53kI GAGCTC 1 cut(s) 2093
Eco57I CTGAAG 6 cut(s) 840, 1955, 2129, 2292, 3243, 3377
EcoICRI GAGCTC 1 cut(s) 2093
EcoO109I RGGNCCY 2 cut(s) 272, 2202
EcoRI GAATTC 1 cut(s) 1280
EcoRII CCWGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
EcoRV GATATC 2 cut(s) 82, 791
EcoT14I CCWWGG 4 cut(s) 123, 2431, 3136, 3203
EcoT22I ATGCAT 1 cut(s) 2608
EcoT38I GRGCYC 2 cut(s) 275, 2095
ErhI CCWWGG 4 cut(s) 123, 2431, 3136, 3203
FalI AAGNNNNNCTT 6 cut(s) 129, 161, 747, 779, 2109, 2141
FaqI GGGAC 4 cut(s) 101, 851, 896, 2628
FauI CCCGC 3 cut(s) 615, 905, 2688
FauNDI CATATG 1 cut(s) 2525
FblI GTMKAC 1 cut(s) 3439
FriOI GRGCYC 2 cut(s) 275, 2095
FseI GGCCGGCC 1 cut(s) 1896
FspBI CTAG 5 cut(s) 438, 1331, 2024, 2327, 2966
GlaI GCGC 5 cut(s) 1112, 1200, 2083, 2777, 2883
GsaI CCCAGC 1 cut(s) 979
GsuI CTGGAG 4 cut(s) 89, 2109, 2843, 2978
HaeIII GGCC 4 cut(s) 273, 1892, 1896, 2298
HapII CCGG 4 cut(s) 187, 1352, 1802, 1893
HhaI GCGC 5 cut(s) 1113, 1201, 2084, 2778, 2884
Hin1I GRCGYC 1 cut(s) 3068
Hin6I GCGC 5 cut(s) 1111, 1199, 2082, 2776, 2882
HinP1I GCGC 5 cut(s) 1111, 1199, 2082, 2776, 2882
HincII GTYRAC 2 cut(s) 2628, 2652
HindII GTYRAC 2 cut(s) 2628, 2652
HindIII AAGCTT 4 cut(s) 71, 2843, 3308, 3377
HpaI GTTAAC 1 cut(s) 2628
HpaII CCGG 4 cut(s) 187, 1352, 1802, 1893
HphI GGTGA 8 cut(s) 12, 515, 1376, 2021, 2522, 3106, 3464, 3492
HpyAV CCTTC 7 cut(s) 88, 245, 946, 1166, 3041, 3185, 3218
HpyCH4IV ACGT 4 cut(s) 9, 1420, 3068, 3245
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 224, 605, 775, 2090, 2276, 2487, 2746, 3540
HpySE526I ACGT 4 cut(s) 9, 1420, 3068, 3245
Hsp92I GRCGYC 1 cut(s) 3068
HspAI GCGC 5 cut(s) 1111, 1199, 2082, 2776, 2882
KroI GCCGGC 2 cut(s) 1801, 1892
KroNI GCCGGC 2 cut(s) 1803, 1894
KspAI GTTAAC 1 cut(s) 2628
Kzo9I GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
LguI GCTCTTC 2 cut(s) 466, 3553
LmnI GCTCC 5 cut(s) 160, 1262, 2090, 2689, 3527
MabI ACCWGGT 1 cut(s) 2579
MaeI CTAG 5 cut(s) 438, 1331, 2024, 2327, 2966
MaeII ACGT 4 cut(s) 9, 1420, 3068, 3245
MalI GATC 8 cut(s) 38, 689, 825, 2013, 2867, 3325, 3499, 3509
MbiI CCGCTC 3 cut(s) 622, 1551, 3109
MboI GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
MfeI CAATTG 2 cut(s) 2401, 3012
MflI RGATCY 1 cut(s) 3323
MhlI GDGCHC 2 cut(s) 275, 2095
MmeI TCCRAC 3 cut(s) 655, 783, 1420
Mph1103I ATGCAT 1 cut(s) 2608
MroNI GCCGGC 2 cut(s) 1801, 1892
MroXI GAANNNNTTC 2 cut(s) 2421, 2763
MslI CAYNNNNRTG 3 cut(s) 266, 528, 3119
MspI CCGG 4 cut(s) 187, 1352, 1802, 1893
MspR9I CCNGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
MunI CAATTG 2 cut(s) 2401, 3012
MvaI CCWGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
MwoI GCNNNNNNNGC 9 cut(s) 202, 224, 605, 775, 2090, 2276, 2487, 2746, 3540
NaeI GCCGGC 2 cut(s) 1803, 1894
NdeI CATATG 1 cut(s) 2525
NdeII GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
NgoMIV GCCGGC 2 cut(s) 1801, 1892
NlaIV GGNNCC 1 cut(s) 273
NmeAIII GCCGAG 1 cut(s) 739
NmuCI GTSAC 5 cut(s) 521, 743, 2482, 2961, 2975
NsiI ATGCAT 1 cut(s) 2608
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1881, 3178
PagI TCATGA 3 cut(s) 220, 690, 918
PciSI GCTCTTC 2 cut(s) 466, 3553
PdiI GCCGGC 2 cut(s) 1803, 1894
PdmI GAANNNNTTC 2 cut(s) 2421, 2763
PfeI GAWTC 5 cut(s) 1496, 1816, 2035, 2437, 3212
PflMI CCANNNNNTGG 3 cut(s) 2432, 2904, 3137
PfoI TCCNGGA 1 cut(s) 2344
Ppu21I YACGTR 1 cut(s) 1421
PpuMI RGGWCCY 1 cut(s) 2202
PshAI GACNNNNGTC 1 cut(s) 659
PsiI TTATAA 1 cut(s) 1566
Psp124BI GAGCTC 1 cut(s) 2095
Psp5II RGGWCCY 1 cut(s) 2202
Psp6I CCWGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
PspFI CCCAGC 1 cut(s) 975
PspGI CCWGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
PspN4I GGNNCC 1 cut(s) 273
PspOMI GGGCCC 1 cut(s) 271
PspPI GGNCC 4 cut(s) 271, 272, 294, 2202
PspPPI RGGWCCY 1 cut(s) 2202
PstI CTGCAG 1 cut(s) 3059
PstNI CAGNNNCTG 1 cut(s) 1978
PsuI RGATCY 1 cut(s) 3323
RigI GGCCGGCC 1 cut(s) 1896
RsaI GTAC 6 cut(s) 1077, 2022, 2231, 2518, 3125, 3491
RsaNI GTAC 6 cut(s) 1076, 2021, 2230, 2517, 3124, 3490
RseI CAYNNNNRTG 3 cut(s) 266, 528, 3119
SacI GAGCTC 1 cut(s) 2095
SapI GCTCTTC 2 cut(s) 466, 3553
Sau3AI GATC 8 cut(s) 36, 687, 823, 2011, 2865, 3323, 3497, 3507
Sau96I GGNCC 4 cut(s) 271, 272, 294, 2202
ScrFI CCNGG 8 cut(s) 276, 402, 527, 1947, 2200, 2346, 2581, 2790
SduI GDGCHC 2 cut(s) 275, 2095
SexAI ACCWGGT 1 cut(s) 2579
SfcI CTRYAG 1 cut(s) 3055
SfuI TTCGAA 2 cut(s) 1881, 3178
SinI GGWCC 2 cut(s) 294, 2202
SmiMI CAYNNNNRTG 3 cut(s) 266, 528, 3119
SmlI CTYRAG 6 cut(s) 206, 2126, 2450, 2617, 3104, 3425
SmoI CTYRAG 6 cut(s) 206, 2126, 2450, 2617, 3104, 3425
SsiI CCGC 9 cut(s) 77, 622, 898, 1549, 2251, 2279, 2695, 3109, 3450
SspMI CTAG 5 cut(s) 438, 1331, 2024, 2327, 2966
SstI GAGCTC 1 cut(s) 2095
StyD4I CCNGG 8 cut(s) 274, 400, 525, 1945, 2198, 2344, 2579, 2788
StyI CCWWGG 4 cut(s) 123, 2431, 3136, 3203
TaiI ACGT 4 cut(s) 12, 1423, 3071, 3248
TaqI TCGA 9 cut(s) 200, 394, 1237, 1770, 1881, 2585, 2996, 3080, 3178
TatI WGTACW 4 cut(s) 2020, 2516, 3123, 3489
TauI GCSGC 1 cut(s) 1551
TfiI GAWTC 5 cut(s) 1496, 1816, 2035, 2437, 3212
TscAI CASTG 4 cut(s) 1983, 2563, 2884, 3257
TseFI GTSAC 5 cut(s) 521, 743, 2482, 2961, 2975
Tsp45I GTSAC 5 cut(s) 521, 743, 2482, 2961, 2975
TspGWI ACGGA 2 cut(s) 1489, 2484
TspRI CASTG 4 cut(s) 1983, 2563, 2884, 3257
Van91I CCANNNNNTGG 3 cut(s) 2432, 2904, 3137
VpaK11BI GGWCC 2 cut(s) 294, 2202
XapI RAATTY 7 cut(s) 1188, 1280, 1542, 2029, 2286, 2314, 3277
XbaI TCTAGA 1 cut(s) 1330
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 3439
XmnI GAANNNNTTC 2 cut(s) 2421, 2763
XspI CTAG 5 cut(s) 438, 1331, 2024, 2327, 2966
ZraI GACGTC 1 cut(s) 3069
Zsp2I ATGCAT 1 cut(s) 2608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.