MD15G1090000.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000400
Physical Location & Seq
Forward (+)
6269379 .. 6270672
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1090000.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAAGCAGCTGCGGGCATTGTTTTGTCCCCGGCTTTGCAAGTGCTCTTTGACAGATTAGCGTCACCGCTCCTACAAAAGGCTTCTGATCTCTTTGGTTACGACGACAACTTCCAGAGCCTGCAGCATGCGTTGGTGCGAGCTCAAGCTACCCTTGAAGTTGCAGAAGAACAGCAGTTCACCAACAAAGCTGCTAAGCTGTGGTTGTTGGACCTCAAGAAGGCAGTTTGCGATGCTCAGGACGTTCTCGACTTCTTTGCCGCTCGCGAAACCTTCAAGATTCGTCCTTTTAGTATTTCAGGAATAGATGATGCAAGTATAAAACCTGAAAAACTCAGGAAGATGCTCCAGCAGTTGGAAAGGACTGTAACTGAGGGATTTCGTATGTTCAATATCAGAGAGCCTACCCAGCTGCTGCCTAGTATCGTAGATCGAGGGTCTATGAAAAGGGAGACTAGCTCTTTTGTTAAGGACACAGAAATACATGGGAGGAAAGAAGAAGGGGAGGAGTTAGTCAAGCTGTTGATATCTTCTGATGCTAAGCAGGTTGAAGGAGGATACGCAGCTTGCGTTTCGATAATTGGTCATGGAGGAATTGGTAAGACGACTCTCGCTCAATTTGCATACAATGATAAGAGGGTAGTGCAACACTTTGATGTTAGGATGTGGATTTTTGTTTCTGTTGACTTCAACATCACAAAGATCATGAAAGGAATTATTGAATCTGTAACAAAGAGTAAGTGTGAGATCACCGGGATTGACGAACTTCAGTCTCAGGTTTGGAATTCCCTGCACAAGAAAAAATTTCTCCTCGTGTTAGATGATGTATGGACTGAAGATCCGGATGATTGGGAAAAGCTAACACCTTTGTTTAGAGAGGGAGTTGATGGATGCAAGATCGTTGTCACCACTCGCAGCAGAAAGATCCCGTTGATGATGGGCAGCTCAATTCAGTATCAGTTGAACGGTTTACCGTTTGGTGATTGCTTGGCTTTGTTTAATCAAAGGGCTTTTGGAAGAGGAGAAGAAGAAAAGTATCCACACCTTGTTCTGATTGGGAAGCAGATTGTCAAAAAATGTGGAGGGTTGCCATTAGCAGTAAAAAGCTTGGGAAGTGTACTGCGCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

378

Amino Acids

42.36

Weight (kDa)

8.1

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 89 8.7e-16 Rx N-terminal domain
NB-ARC PF00931 184 - 339 1.1e-33 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB7I CCANNNNNTGG 1 cut(s) 355
AccBSI CCGCTC 2 cut(s) 70, 263
AccII CGCG 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 841
AciI CCGC 3 cut(s) 14, 68, 261
AclWI GGATC 2 cut(s) 833, 919
AcsI RAATTY 2 cut(s) 784, 803
AcuI CTGAAG 2 cut(s) 752, 855
AfaI GTAC 1 cut(s) 1119
AfiI CCNNNNNNNGG 3 cut(s) 79, 220, 355
AgsI TTSAA 7 cut(s) 158, 277, 391, 551, 691, 722, 964
Alw21I GWGCWC 2 cut(s) 48, 145
Alw26I GTCTC 2 cut(s) 446, 777
AlwI GGATC 2 cut(s) 833, 919
AlwNI CAGNNNCTG 2 cut(s) 121, 415
Aor13HI TCCGGA 1 cut(s) 841
ApeKI GCWGC 9 cut(s) 8, 11, 124, 191, 412, 415, 563, 915, 942
ApoI RAATTY 2 cut(s) 784, 803
AspLEI GCGC 1 cut(s) 1125
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 2 cut(s) 32, 754
AsuHPI GGTGA 5 cut(s) 57, 172, 742, 898, 992
AvaII GGWCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 812
Bbv12I GWGCWC 2 cut(s) 48, 145
BbvI GCAGC 8 cut(s) 20, 136, 178, 399, 402, 575, 927, 954
BccI CCATC 2 cut(s) 881, 931
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciVI GTATCC 2 cut(s) 551, 1047
BcnI CCSGG 2 cut(s) 32, 754
BcoDI GTCTC 2 cut(s) 446, 777
BfaI CTAG 2 cut(s) 420, 456
BfmI CTRYAG 1 cut(s) 122
BfuAI ACCTGC 1 cut(s) 535
BfuI GTATCC 2 cut(s) 551, 1047
BlpI GCTNAGC 2 cut(s) 195, 540
Bme1390I CCNGG 2 cut(s) 32, 754
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 32, 754
BmsI GCATC 5 cut(s) 223, 301, 333, 526, 881
BplI GAGNNNNNCTC 2 cut(s) 443, 475
BpmI CTGGAG 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 237
Bpu1102I GCTNAGC 2 cut(s) 195, 540
BpuEI CTTGAG 2 cut(s) 129, 200
BpuMI CCSGG 2 cut(s) 32, 754
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 841
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 220, 355
BseAI TCCGGA 1 cut(s) 841
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 3 cut(s) 669, 850, 896
BseLI CCNNNNNNNGG 3 cut(s) 79, 220, 355
BseMII CTCAG 4 cut(s) 251, 349, 363, 788
BseRI GAGGAG 3 cut(s) 521, 800, 1035
BseXI GCAGC 8 cut(s) 20, 136, 178, 399, 402, 575, 927, 954
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 48, 145
BsiSI CCGG 3 cut(s) 32, 753, 842
BslFI GGGAC 1 cut(s) 13
BslI CCNNNNNNNGG 3 cut(s) 79, 220, 355
BsmAI GTCTC 2 cut(s) 446, 777
BsmFI GGGAC 1 cut(s) 13
Bsp1286I GDGCHC 2 cut(s) 48, 145
Bsp13I TCCGGA 1 cut(s) 841
Bsp143I GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
Bsp1720I GCTNAGC 2 cut(s) 195, 540
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 3 cut(s) 14, 68, 261
BspCNI CTCAG 4 cut(s) 250, 348, 364, 787
BspEI TCCGGA 1 cut(s) 841
BspFNI CGCG 1 cut(s) 267
BspHI TCATGA 1 cut(s) 705
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 535
BspPI GGATC 2 cut(s) 833, 919
BsrBI CCGCTC 2 cut(s) 70, 263
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
BssSI CACGAG 1 cut(s) 812
Bst2BI CACGAG 1 cut(s) 812
Bst4CI ACNGT 3 cut(s) 367, 968, 975
Bst6I CTCTTC 1 cut(s) 1012
BstC8I GCNNGC 6 cut(s) 16, 122, 129, 141, 265, 568
BstDEI CTNAG 6 cut(s) 195, 237, 335, 372, 540, 774
BstENI CCTNNNNNAGG 2 cut(s) 77, 218
BstF5I GGATG 3 cut(s) 669, 850, 896
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 1125
BstKTI GATC 7 cut(s) 91, 433, 705, 750, 841, 900, 927
BstMAI GTCTC 2 cut(s) 446, 777
BstMBI GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
BstMWI GCNNNNNNNGC 2 cut(s) 409, 620
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 30, 752
BstSFI CTRYAG 1 cut(s) 122
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 8 cut(s) 20, 136, 178, 399, 402, 575, 927, 954
BstX2I RGATCY 2 cut(s) 838, 924
BstYI RGATCY 2 cut(s) 838, 924
BsuI GTATCC 2 cut(s) 551, 1047
BtgZI GCGATG 1 cut(s) 246
BtsCI GGATG 3 cut(s) 669, 850, 896
BtuMI TCGCGA 1 cut(s) 267
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 6 cut(s) 16, 122, 129, 141, 265, 568
CaiI CAGNNNCTG 2 cut(s) 121, 415
CciI TCATGA 1 cut(s) 705
CfoI GCGC 1 cut(s) 1125
Cfr13I GGNCC 1 cut(s) 211
CseI GACGC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 1118
CspCI CAANNNNNGTGG 2 cut(s) 1060, 1095
CviAII CATG 4 cut(s) 128, 485, 587, 706
CviQI GTAC 1 cut(s) 1118
DdeI CTNAG 6 cut(s) 195, 237, 335, 372, 540, 774
DpnI GATC 7 cut(s) 90, 432, 704, 749, 840, 899, 926
DpnII GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
DraI TTTAAA 1 cut(s) 1129
Eam1104I CTCTTC 1 cut(s) 1012
EarI CTCTTC 1 cut(s) 1012
Ecl136II GAGCTC 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 143
Eco57I CTGAAG 2 cut(s) 752, 855
EcoICRI GAGCTC 1 cut(s) 143
EcoNI CCTNNNNNAGG 2 cut(s) 77, 218
EcoRI GAATTC 1 cut(s) 784
EcoRV GATATC 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 145
FaeI CATG 4 cut(s) 131, 488, 590, 709
FaiI YATR 9 cut(s) 129, 320, 386, 443, 486, 588, 625, 707, 829
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 1 cut(s) 13
FatI CATG 4 cut(s) 127, 484, 586, 705
FauI CCCGC 1 cut(s) 7
FokI GGATG 3 cut(s) 676, 857, 903
FriOI GRGCYC 1 cut(s) 145
FspBI CTAG 2 cut(s) 420, 456
GlaI GCGC 1 cut(s) 1124
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 1 cut(s) 332
HapII CCGG 3 cut(s) 32, 753, 842
HgaI GACGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 1125
Hin1II CATG 4 cut(s) 131, 488, 590, 709
Hin6I GCGC 1 cut(s) 1123
HinP1I GCGC 1 cut(s) 1123
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HindIII AAGCTT 1 cut(s) 1105
HinfI GANTC 3 cut(s) 280, 607, 722
HpaII CCGG 3 cut(s) 32, 753, 842
HphI GGTGA 5 cut(s) 57, 172, 742, 898, 992
Hpy166II GTNNAC 4 cut(s) 180, 685, 971, 1118
Hpy188I TCNGA 4 cut(s) 88, 398, 535, 1053
Hpy8I GTNNAC 4 cut(s) 180, 685, 971, 1118
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 4 cut(s) 214, 283, 494, 545
HpyCH4III ACNGT 3 cut(s) 367, 968, 975
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 8 cut(s) 40, 124, 164, 314, 623, 646, 793, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 409, 620
HpyF3I CTNAG 6 cut(s) 195, 237, 335, 372, 540, 774
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 4 cut(s) 131, 488, 590, 709
HspAI GCGC 1 cut(s) 1123
Kpn2I TCCGGA 1 cut(s) 841
Kzo9I GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
LmnI GCTCC 2 cut(s) 75, 351
Lsp1109I GCAGC 8 cut(s) 20, 136, 178, 399, 402, 575, 927, 954
LweI GCATC 5 cut(s) 223, 301, 333, 526, 881
MaeI CTAG 2 cut(s) 420, 456
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 5 cut(s) 63, 98, 367, 727, 904
MalI GATC 7 cut(s) 90, 432, 704, 749, 840, 899, 926
MbiI CCGCTC 2 cut(s) 70, 263
MboI GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
MboII GAAGA 8 cut(s) 179, 352, 509, 522, 848, 1029, 1037, 1040
MflI RGATCY 2 cut(s) 838, 924
MhlI GDGCHC 2 cut(s) 48, 145
MluCI AATT 7 cut(s) 579, 594, 617, 714, 784, 803, 948
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 2 cut(s) 189, 336
MroI TCCGGA 1 cut(s) 841
MseI TTAA 3 cut(s) 468, 999, 1128
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 2 cut(s) 11, 412
MspI CCGG 3 cut(s) 32, 753, 842
MspR9I CCNGG 2 cut(s) 32, 754
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 2 cut(s) 409, 620
NciI CCSGG 2 cut(s) 32, 754
NdeII GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
NlaIII CATG 4 cut(s) 131, 488, 590, 709
NmuCI GTSAC 2 cut(s) 63, 904
NruI TCGCGA 1 cut(s) 267
NspI RCATGY 1 cut(s) 131
PaeI GCATGC 1 cut(s) 131
PagI TCATGA 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PfeI GAWTC 2 cut(s) 280, 722
PflMI CCANNNNNTGG 1 cut(s) 355
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
Psp124BI GAGCTC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 408
PspPI GGNCC 1 cut(s) 211
PstI CTGCAG 1 cut(s) 126
PstNI CAGNNNCTG 2 cut(s) 121, 415
PsuI RGATCY 2 cut(s) 838, 924
PvuII CAGCTG 2 cut(s) 11, 412
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 1 cut(s) 1119
RsaNI GTAC 1 cut(s) 1118
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 3 cut(s) 468, 999, 1128
Sau3AI GATC 7 cut(s) 88, 430, 702, 747, 838, 897, 924
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 2 cut(s) 32, 754
SduI GDGCHC 2 cut(s) 48, 145
SfaNI GCATC 5 cut(s) 223, 301, 333, 526, 881
SfcI CTRYAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 144, 215
SmoI CTYRAG 2 cut(s) 144, 215
SphI GCATGC 1 cut(s) 131
Sse9I AATT 7 cut(s) 579, 594, 617, 714, 784, 803, 948
SsiI CCGC 3 cut(s) 14, 68, 261
SspMI CTAG 2 cut(s) 420, 456
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 2 cut(s) 30, 752
TaaI ACNGT 3 cut(s) 367, 968, 975
TaiI ACGT 1 cut(s) 246
TaqI TCGA 3 cut(s) 249, 433, 575
TasI AATT 7 cut(s) 579, 594, 617, 714, 784, 803, 948
TatI WGTACW 1 cut(s) 1117
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 280, 722
Tru1I TTAA 3 cut(s) 468, 999, 1128
Tru9I TTAA 3 cut(s) 468, 999, 1128
TseFI GTSAC 2 cut(s) 63, 904
TseI GCWGC 9 cut(s) 8, 11, 124, 191, 412, 415, 563, 915, 942
Tsp45I GTSAC 2 cut(s) 63, 904
TspDTI ATGAA 2 cut(s) 458, 722
Van91I CCANNNNNTGG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 211
XagI CCTNNNNNAGG 2 cut(s) 77, 218
XapI RAATTY 2 cut(s) 784, 803
XceI RCATGY 1 cut(s) 131
XspI CTAG 2 cut(s) 420, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.