Rw1G018020

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000400
Physical Location & Seq
Reverse (-)
39711184 .. 39716746
5563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G018020.1

Sequence Viewer

Length: 3552 bp
ATGGCAGACGTAGTTTTATCACCAGCTTTGCAAGTGATCTTTGACAGAGTAGCATCCCCAGTCCTACAAAAGCTTGCGGATATCTGGGACATGAAGGACAACCTCCAGAGCCTACAACATGCCTTGGTCATGGTTCAAGCTATTCTTGAAGATGCAGAGGAGCAACAACTGACCAACAAAGCCGTCCGGCTTTGGCTGTCGAAGCTCAAGAACGCAGCTCATGATGCTGATGACCTTCTGGACTTCGTTGCTGCTAAAGACATCATTATGGGCCCTGGAAAATTATTAAAAAACCCAATTCCAAAATACATGGATGCTGATAAAGTCAGAAAGATGCTTTTGGCTTTAGAAATGACAGTAAACGAGGGTCTATTGAAGTTTAATTTTCGAGAGCCTGGTTCAGTAGATAGGCAATCTGATAAAAGGGAGACTAGCTCTTTTGTTTATGAGTCAGAAATATATGGAAGAGCTGATGACAAAGAGAAGTTAGTGAAACTGTTACTATCTTCTGAGTGTCACCAGGATGGATATGCTATGTGTATTGACTGTATTCCGATAATTGGTATTGGAGGAATTGGCAAGACGACTCTTGCCCAGTTGGCATATAATGATGAGCGGGTAATCCAACATTTTGATATTAGGCTGTGGACTTTTGTCTCGGATGATTTCAATATCAAGAAGATCATGAAGTCAATTATTGAGTCTGTAACAAGGGAAAATTGCAAGTTCTCGGAGAGTGACTTACTTCAAGCTCGGCTTTGGCATTTGCTGCACAACAAAAGATATCTAATTGTGTTGGATGATGTTTGGACTGAAGATCAAGATGACTGGGACAAACTAAGACCTTTGTTTAGAGGAGGTGTTGATGGATGCAAAATCATTATCACTTCCCGCAATAAAAAAGTCCCATTCATGATGGACTCCCCAACTTCGGCATATTATTTGAAGGGTTTGATGGAGGATGACTGCTGGGCTTTGTTCAAGCAACGAGCTTTTGGACGAGGAGAAGAAGAGAAGTATGCCAACCTCTGCCTGATTGGAAAGAAGATTGCGAAGAAATGTGGTGGTGTACCATTGGCAGCCAAAAGTTTGGGAAGTGTTATGCGCTTTAAAAGAGAAGAACGGCAATGGTTGTTTATGCAAAATAGTGAACTGTGGGAAGTAGAAGGATGGAAACAAAGAATTCTACCTGCGCTTATGTTGAGTTATCTTCATCTGCCATCACATCTTCGACAATGCTTTGCGTTCTGCTCCATATTTCCTAAAAATTATGAATTCAAGAAGCATAAATTAGTTCTCTTGTGGATGGCAGAGGGCTTAATTCTAGAAGAAAGTAGCAAGCGACCGGAAGACATTGGTGATGAATACTTTTCTGATTTGCTGTGGATGTCTTTCTTTCAAGAAGTAGAGTTACGTGATGGTGGCAGCACAGTTGGATACAAAATGAATGATGTCATTCATGACCTTGCACGATATGTTTCTGGAAATGAATCCGTTATGCTTGAACAAGGTCTTCCACCGAGTAATCCAGCACAAATTCGCCGCTCATCCATTATTTATAAATACAGGGAAGTTACAATTCCAGAAGCTCTCTTTGAAGCAGCACATTTACGAACTGTCTTATTGATTGGAGAATATGGACTTCTAAAAGACAGTAGTAAAATGTTATTAAGTTGTATATACTTGCGTGTGCTGGACTTGAACAACTGTGATGTTCATTTTTTGCCGTCATCAATAGGTGGGTTGATATGTTTGAGGTATCTCGACTTGTCTTATACACCTATCTCCAAGTTGCCGGCAACCACAAGGAATCTCTGTTCATTGCAGACTTTAAACCTCTCTGGATGTCATAATCTTAGAGTCTTGCCAGACTTCGAAGTAATGGCCGGCCTAAGACATCTCAATATAGCTGGATGTGATAGGTTGTGGTATATACCTCCTGGTATTAAAATGTTACTTCAACTTCAGACACTGCCATTATATTTCGTTCAAAAGAGGGTCGGTGATCTTGGTGTACTAGAGAATTTGAATCTTTATGGGGAGTTGAATATTACACGCTTGGAGAATGTGAGAAGCGCAACTGGAGCTCAATCATCTGGACTGAAGATGAAAGAAAATCTTGAGTCGTTGGGATTATGTTGGGGAGATTTTGAAGATGTGAACGACAGGAAAAAATCATTGAAGAAACTAGCCAAGTCCCCAGCCAAAAGGTTTTTTTTAGAAGCAGGGGTACGGTGGCATGAACCTGATGCTGAAGAGGCGATAGAAATTCTTGATGGCCTGAAACCACACAAGAATTTGAAAAAGCTAGTTATACATGGTTATCCAGGAATCAGATTTCCAGATTGGACTCTCCCAAATGTAATTGCAGTTGATTTGTCCAATTGTAAGAACTGTGAATATCTTCCAGCCCTTGGGAATCTTCTTCTACTCAAGACTCTTTCTCTGCACGGAATGGAGAGCGTGACACGCATTGGTTTTGAGTTCTATGGTGATAGTACAGACATATGCTTTTCATCACTTGAAGAACTATCGCTCAGTGATTTTCCGAACTTGGAAACCTGGTCGAGTGCAAATAATGAAAATGCATTTCCGAGCTTGAGGAAGTTAACCATAAATAGGTGTCCCAAGTTGACACATATCCCATTTTGTTTGTCTCTTCAACATTTGGAGCTGCGGGATTGTAATCCATCTTCAATCTTCATTGGAGGTTTAACTTTGCTGACAATGCTTGTAATAGAAAAACTTCCAGAGCTGCGCTCTCTACCACCAGGGCTTTCTGCATCAGCTAATCTGTCTTCTCTGGAGGTCTTATCTTGCCCCAAGCTTTGTTCCCTGCCTTTTGAGATCGGAAACCTCACTGCGCTGAAATCATTAACCATTCGTTGGTGTGATGAGTTGTCCTCTCTGCCACAGAGTTTGCAGAACCTCAAAGCTCTGGAGTCACTAGAGATTAGTGACTGTCATAGTATAATCTCGATGCCAGATGGTGCAATTGGGGGTTTGAGTTCCCTTCAAACTTTGTCCATTGAGAACTGCAGTAATCTGACGTCTTTGTCATCGAGTTTGGAACAACTTGCTTTCCTTGATCGGTTCACCATTATGTACTGTCCAAACCTTGGTTCTTTCGCTGAGGGTGTGCAACACCTCTCAACCCTTCGAAGTTTGTCTATATTGAGCTGTCCTTGGTTTGATTCTTTGCCTGAAGGGTTGCAAATGGTCAAGACGTTGCAGTGTCTTGAAATTAATAGCTGCCCGAATTTCACAGCTTTGCCAGAGTGGGTTGAGAAGCTTGCTTCACTCAGATCTTTGACCATTTTTGATTGTGCTAATTTGAAATTGTTACCACAAAGTCTAAAGCTTCTCACTAAACTTCAGCACCTCTCTATTCAAGAATGTCCTGAGCTTGAGAGAAGATGTAGACAAGGAAGCGGTGAGGAATGGCTTAAAATATCTCATGTTCCACATAAGTACATTGGATCACCGGAGATCAGGCAGTCTGGTAGTGGAGCAAGCACATCCAGCAGCTCTTCTTTTGATACATCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1183

Amino Acids

133.41

Weight (kDa)

6.05

Isoelectric Point (pI)

54.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 84 3.6e-17 Rx N-terminal domain
NB-ARC PF00931 175 - 333 7.2e-36 NB-ARC domain
WHD_DRP PF23559 419 - 490 1.4e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 554 - 890 9.3e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 671 - 819 5.7e-27 R13L1/DRL21 LRRs
LRR_14 PF23598 967 - 1131 3.1e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1560
AasI GACNNNNNNGTC 1 cut(s) 3055
AatII GACGTC 1 cut(s) 3053
Acc36I ACCTGC 1 cut(s) 1198
AccB7I CCANNNNNTGG 3 cut(s) 2414, 2886, 3119
AccBSI CCGCTC 2 cut(s) 616, 1545
AccI GTMKAC 1 cut(s) 3421
AciI CCGC 6 cut(s) 77, 616, 892, 1543, 2677, 3432
AclWI GGATC 1 cut(s) 3487
AcoI YGGCCR 1 cut(s) 1884
AcsI RAATTY 7 cut(s) 1182, 1274, 1536, 2023, 2268, 2296, 3259
AcuI CTGAAG 6 cut(s) 834, 1949, 2123, 2274, 3225, 3359
AcyI GRCGYC 1 cut(s) 3050
AfaI GTAC 6 cut(s) 1071, 2016, 2232, 2500, 3107, 3473
AfiI CCNNNNNNNGG 6 cut(s) 560, 931, 2414, 2619, 2886, 3119
AjnI CCWGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
AjuI GAANNNNNNNTTGG 2 cut(s) 3106, 3138
AloI GAACNNNNNNTCC 2 cut(s) 1801, 1833
Alw21I GWGCWC 1 cut(s) 2089
Alw26I GTCTC 3 cut(s) 422, 661, 2661
AlwI GGATC 1 cut(s) 3487
AlwNI CAGNNNCTG 1 cut(s) 1972
AoxI GGCC 4 cut(s) 271, 1884, 1888, 2278
ApaI GGGCCC 1 cut(s) 275
ApoI RAATTY 7 cut(s) 1182, 1274, 1536, 2023, 2268, 2296, 3259
AseI ATTAAT 1 cut(s) 3246
Asp700I GAANNNNTTC 2 cut(s) 2403, 2745
AspLEI GCGC 5 cut(s) 1107, 1195, 2078, 2760, 2866
AspS9I GGNCC 2 cut(s) 271, 272
AsuHPI GGTGA 8 cut(s) 12, 509, 1370, 2015, 2504, 3088, 3446, 3474
AsuII TTCGAA 2 cut(s) 1875, 3160
BaeGI GKGCMC 1 cut(s) 275
BaeI ACNNNNGTAYC 1 cut(s) 3531
BanII GRGCYC 2 cut(s) 275, 2089
BarI GAAGNNNNNNTAC 6 cut(s) 1395, 1427, 2214, 2246, 2677, 2709
BbsI GAAGAC 3 cut(s) 1356, 1505, 2790
Bbv12I GWGCWC 1 cut(s) 2089
BbvCI CCTCAGC 1 cut(s) 3132
BceAI ACGGC 3 cut(s) 167, 1139, 1711
BciT130I CCWGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
BciVI GTATCC 1 cut(s) 1430
BcoDI GTCTC 3 cut(s) 422, 661, 2661
BfaI CTAG 6 cut(s) 432, 1325, 2018, 2189, 2309, 2948
BfmI CTRYAG 1 cut(s) 3037
BfuAI ACCTGC 1 cut(s) 1198
BfuI GTATCC 1 cut(s) 1430
BglI GCCNNNNNGGC 1 cut(s) 599
BglII AGATCT 1 cut(s) 3305
Bme1390I CCNGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
BmgT120I GGNCC 2 cut(s) 271, 272
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
BmrI ACTGGG 3 cut(s) 53, 589, 838
BmsI GCATC 9 cut(s) 62, 142, 214, 304, 324, 860, 2239, 2792, 2970
BmuI ACTGGG 3 cut(s) 53, 589, 838
BoxI GACNNNNGTC 1 cut(s) 653
BpiI GAAGAC 3 cut(s) 1356, 1505, 2790
BplI GAGNNNNNCTC 6 cut(s) 419, 451, 2744, 2776, 2888, 2920
BpmI CTGGAG 4 cut(s) 89, 2103, 2825, 2960
Bpu10I CCTNAGC 2 cut(s) 3132, 3402
Bpu14I TTCGAA 2 cut(s) 1875, 3160
BpuEI CTTGAG 5 cut(s) 191, 2141, 2417, 2620, 3428
BsaAI YACGTR 1 cut(s) 1415
BsaBI GATNNNNATC 1 cut(s) 2685
BsaHI GRCGYC 1 cut(s) 3050
BsaJI CCNNGG 6 cut(s) 123, 274, 2413, 2771, 3118, 3185
BsaWI WCCGGW 2 cut(s) 1345, 3484
Bsc4I CCNNNNNNNGG 6 cut(s) 560, 931, 2414, 2619, 2886, 3119
Bse118I RCCGGY 2 cut(s) 1795, 1886
Bse1I ACTGG 4 cut(s) 59, 595, 833, 2086
Bse3DI GCAATG 2 cut(s) 1133, 1820
Bse8I GATNNNNATC 1 cut(s) 2685
BseBI CCWGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
BseDI CCNNGG 6 cut(s) 123, 274, 2413, 2771, 3118, 3185
BseJI GATNNNNATC 1 cut(s) 2685
BseLI CCNNNNNNNGG 6 cut(s) 560, 931, 2414, 2619, 2886, 3119
BseMI GCAATG 2 cut(s) 1133, 1820
BseMII CTCAG 5 cut(s) 501, 2551, 3123, 3316, 3393
BseNI ACTGG 4 cut(s) 59, 595, 833, 2086
BseRI GAGGAG 3 cut(s) 173, 870, 1017
BseSI GKGCMC 1 cut(s) 275
BseYI CCCAGC 2 cut(s) 969, 2200
BsgI GTGCAG 2 cut(s) 755, 2432
Bsh1285I CGRYCG 1 cut(s) 1346
BshFI GGCC 4 cut(s) 273, 1886, 1890, 2280
BsiEI CGRYCG 1 cut(s) 1346
BsiHKAI GWGCWC 1 cut(s) 2089
BsiSI CCGG 5 cut(s) 187, 1346, 1796, 1887, 3485
BslFI GGGAC 5 cut(s) 101, 845, 890, 2182, 2610
BslI CCNNNNNNNGG 6 cut(s) 560, 931, 2414, 2619, 2886, 3119
BsmAI GTCTC 3 cut(s) 422, 661, 2661
BsmFI GGGAC 5 cut(s) 101, 845, 890, 2182, 2610
BsnI GGCC 4 cut(s) 273, 1886, 1890, 2280
Bsp119I TTCGAA 2 cut(s) 1875, 3160
Bsp120I GGGCCC 1 cut(s) 271
Bsp1286I GDGCHC 2 cut(s) 275, 2089
Bsp143I GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
BspACI CCGC 6 cut(s) 77, 616, 892, 1543, 2677, 3432
BspANI GGCC 4 cut(s) 273, 1886, 1890, 2280
BspCNI CTCAG 5 cut(s) 502, 2550, 3124, 3315, 3394
BspHI TCATGA 4 cut(s) 220, 684, 912, 1459
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 3041
BspMI ACCTGC 1 cut(s) 1198
BspPI GGATC 1 cut(s) 3487
BspQI GCTCTTC 2 cut(s) 460, 3535
BspT104I TTCGAA 2 cut(s) 1875, 3160
BsrBI CCGCTC 2 cut(s) 616, 1545
BsrDI GCAATG 2 cut(s) 1133, 1820
BsrFI RCCGGY 2 cut(s) 1795, 1886
BsrI ACTGG 4 cut(s) 59, 595, 833, 2086
BssAI RCCGGY 2 cut(s) 1795, 1886
BssECI CCNNGG 6 cut(s) 123, 274, 2413, 2771, 3118, 3185
BssMI GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
BssNI GRCGYC 1 cut(s) 3050
BssT1I CCWWGG 4 cut(s) 123, 2413, 3118, 3185
Bst2UI CCWGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
Bst6I CTCTTC 5 cut(s) 460, 1005, 2250, 2664, 3535
BstACI GRCGYC 1 cut(s) 3050
BstAPI GCANNNNNTGC 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 1415
BstBI TTCGAA 2 cut(s) 1875, 3160
BstC8I GCNNGC 6 cut(s) 75, 1340, 1797, 1888, 3294, 3514
BstDEI CTNAG 8 cut(s) 510, 839, 1856, 1892, 2537, 3132, 3302, 3402
BstHHI GCGC 5 cut(s) 1107, 1195, 2078, 2760, 2866
BstKTI GATC 9 cut(s) 39, 684, 820, 2008, 2850, 3091, 3308, 3482, 3492
BstMAI GTCTC 3 cut(s) 422, 661, 2661
BstMBI GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
BstMCI CGRYCG 1 cut(s) 1346
BstMWI GCNNNNNNNGC 9 cut(s) 202, 224, 599, 769, 2084, 2258, 2469, 2728, 3522
BstNI CCWGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
BstNSI RCATGY 1 cut(s) 122
BstPAI GACNNNNGTC 1 cut(s) 653
BstSCI CCNGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
BstSFI CTRYAG 1 cut(s) 3037
BstSLI GKGCMC 1 cut(s) 275
BstV2I GAAGAC 3 cut(s) 1356, 1505, 2790
BstX2I RGATCY 1 cut(s) 3305
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 1 cut(s) 3305
BsuI GTATCC 1 cut(s) 1430
BsuRI GGCC 4 cut(s) 273, 1886, 1890, 2280
BtsI GCAGTG 3 cut(s) 1970, 2859, 3239
BtsIMutI CAGTG 4 cut(s) 1970, 2545, 2859, 3239
BveI ACCTGC 1 cut(s) 1198
Cac8I GCNNGC 6 cut(s) 75, 1340, 1797, 1888, 3294, 3514
CaiI CAGNNNCTG 1 cut(s) 1972
CciI TCATGA 4 cut(s) 220, 684, 912, 1459
CfoI GCGC 5 cut(s) 1107, 1195, 2078, 2760, 2866
Cfr10I RCCGGY 2 cut(s) 1795, 1886
Cfr13I GGNCC 2 cut(s) 271, 272
CsiI ACCWGGT 1 cut(s) 2561
Csp6I GTAC 6 cut(s) 1070, 2015, 2231, 2499, 3106, 3472
CspCI CAANNNNNGTGG 2 cut(s) 2901, 2936
CviQI GTAC 6 cut(s) 1070, 2015, 2231, 2499, 3106, 3472
DdeI CTNAG 8 cut(s) 510, 839, 1856, 1892, 2537, 3132, 3302, 3402
DpnI GATC 9 cut(s) 38, 683, 819, 2007, 2849, 3090, 3307, 3481, 3491
DpnII GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
DraI TTTAAA 2 cut(s) 1111, 1833
DrdI GACNNNNNNGTC 1 cut(s) 3055
DseDI GACNNNNNNGTC 1 cut(s) 3055
EaeI YGGCCR 1 cut(s) 1884
Eam1104I CTCTTC 5 cut(s) 460, 1005, 2250, 2664, 3535
EarI CTCTTC 5 cut(s) 460, 1005, 2250, 2664, 3535
Ecl136II GAGCTC 1 cut(s) 2087
Eco130I CCWWGG 4 cut(s) 123, 2413, 3118, 3185
Eco24I GRGCYC 2 cut(s) 275, 2089
Eco32I GATATC 2 cut(s) 82, 785
Eco53kI GAGCTC 1 cut(s) 2087
Eco57I CTGAAG 6 cut(s) 834, 1949, 2123, 2274, 3225, 3359
EcoICRI GAGCTC 1 cut(s) 2087
EcoO109I RGGNCCY 1 cut(s) 272
EcoRI GAATTC 2 cut(s) 1182, 1274
EcoRII CCWGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
EcoRV GATATC 2 cut(s) 82, 785
EcoT14I CCWWGG 4 cut(s) 123, 2413, 3118, 3185
EcoT22I ATGCAT 1 cut(s) 2590
EcoT38I GRGCYC 2 cut(s) 275, 2089
ErhI CCWWGG 4 cut(s) 123, 2413, 3118, 3185
FalI AAGNNNNNCTT 6 cut(s) 129, 161, 741, 773, 2103, 2135
FaqI GGGAC 5 cut(s) 101, 845, 890, 2182, 2610
FauI CCCGC 3 cut(s) 609, 899, 2670
FauNDI CATATG 1 cut(s) 2507
FblI GTMKAC 1 cut(s) 3421
FriOI GRGCYC 2 cut(s) 275, 2089
FseI GGCCGGCC 1 cut(s) 1890
FspBI CTAG 6 cut(s) 432, 1325, 2018, 2189, 2309, 2948
GlaI GCGC 5 cut(s) 1106, 1194, 2077, 2759, 2865
GsaI CCCAGC 2 cut(s) 973, 2204
GsuI CTGGAG 4 cut(s) 89, 2103, 2825, 2960
HaeIII GGCC 4 cut(s) 273, 1886, 1890, 2280
HapII CCGG 5 cut(s) 187, 1346, 1796, 1887, 3485
HhaI GCGC 5 cut(s) 1107, 1195, 2078, 2760, 2866
Hin1I GRCGYC 1 cut(s) 3050
Hin6I GCGC 5 cut(s) 1105, 1193, 2076, 2758, 2864
HinP1I GCGC 5 cut(s) 1105, 1193, 2076, 2758, 2864
HincII GTYRAC 2 cut(s) 2610, 2634
HindII GTYRAC 2 cut(s) 2610, 2634
HindIII AAGCTT 4 cut(s) 71, 2825, 3290, 3359
HpaI GTTAAC 1 cut(s) 2610
HpaII CCGG 5 cut(s) 187, 1346, 1796, 1887, 3485
HphI GGTGA 8 cut(s) 12, 509, 1370, 2015, 2504, 3088, 3446, 3474
HpyAV CCTTC 7 cut(s) 88, 245, 940, 1160, 3023, 3167, 3200
HpyCH4IV ACGT 4 cut(s) 9, 1414, 3050, 3227
HpyF10VI GCNNNNNNNGC 9 cut(s) 202, 224, 599, 769, 2084, 2258, 2469, 2728, 3522
HpyF3I CTNAG 8 cut(s) 510, 839, 1856, 1892, 2537, 3132, 3302, 3402
HpySE526I ACGT 4 cut(s) 9, 1414, 3050, 3227
Hsp92I GRCGYC 1 cut(s) 3050
HspAI GCGC 5 cut(s) 1105, 1193, 2076, 2758, 2864
KroI GCCGGC 2 cut(s) 1795, 1886
KroNI GCCGGC 2 cut(s) 1797, 1888
KspAI GTTAAC 1 cut(s) 2610
Kzo9I GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
LguI GCTCTTC 2 cut(s) 460, 3535
LmnI GCTCC 5 cut(s) 160, 1256, 2084, 2671, 3509
LweI GCATC 9 cut(s) 62, 142, 214, 304, 324, 860, 2239, 2792, 2970
MabI ACCWGGT 1 cut(s) 2561
MaeI CTAG 6 cut(s) 432, 1325, 2018, 2189, 2309, 2948
MaeII ACGT 4 cut(s) 9, 1414, 3050, 3227
MalI GATC 9 cut(s) 38, 683, 819, 2007, 2849, 3090, 3307, 3481, 3491
MbiI CCGCTC 2 cut(s) 616, 1545
MboI GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
MfeI CAATTG 2 cut(s) 2383, 2994
MflI RGATCY 1 cut(s) 3305
MhlI GDGCHC 2 cut(s) 275, 2089
MlyI GAGTC 9 cut(s) 458, 580, 710, 914, 1869, 2132, 2344, 2431, 2951
MmeI TCCRAC 3 cut(s) 649, 777, 1414
Mph1103I ATGCAT 1 cut(s) 2590
MroNI GCCGGC 2 cut(s) 1795, 1886
MroXI GAANNNNTTC 2 cut(s) 2403, 2745
MslI CAYNNNNRTG 3 cut(s) 266, 522, 3101
MspI CCGG 5 cut(s) 187, 1346, 1796, 1887, 3485
MspR9I CCNGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
MunI CAATTG 2 cut(s) 2383, 2994
MvaI CCWGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
MwoI GCNNNNNNNGC 9 cut(s) 202, 224, 599, 769, 2084, 2258, 2469, 2728, 3522
NaeI GCCGGC 2 cut(s) 1797, 1888
NdeI CATATG 1 cut(s) 2507
NdeII GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
NgoMIV GCCGGC 2 cut(s) 1795, 1886
NlaIV GGNNCC 1 cut(s) 273
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 5 cut(s) 515, 737, 2464, 2943, 2957
NsiI ATGCAT 1 cut(s) 2590
NspI RCATGY 1 cut(s) 122
NspV TTCGAA 2 cut(s) 1875, 3160
PagI TCATGA 4 cut(s) 220, 684, 912, 1459
PciSI GCTCTTC 2 cut(s) 460, 3535
PdiI GCCGGC 2 cut(s) 1797, 1888
PdmI GAANNNNTTC 2 cut(s) 2403, 2745
PfeI GAWTC 6 cut(s) 1490, 1810, 2029, 2331, 2419, 3194
PflMI CCANNNNNTGG 3 cut(s) 2414, 2886, 3119
PfoI TCCNGGA 1 cut(s) 2326
PleI GAGTC 9 cut(s) 457, 580, 709, 914, 1868, 2131, 2344, 2431, 2950
PpsI GAGTC 9 cut(s) 457, 580, 709, 914, 1868, 2131, 2344, 2431, 2950
Ppu21I YACGTR 1 cut(s) 1415
PshAI GACNNNNGTC 1 cut(s) 653
PshBI ATTAAT 1 cut(s) 3246
PsiI TTATAA 1 cut(s) 1560
Psp124BI GAGCTC 1 cut(s) 2089
Psp6I CCWGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
PspFI CCCAGC 2 cut(s) 969, 2200
PspGI CCWGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
PspN4I GGNNCC 1 cut(s) 273
PspOMI GGGCCC 1 cut(s) 271
PspPI GGNCC 2 cut(s) 271, 272
PstI CTGCAG 1 cut(s) 3041
PstNI CAGNNNCTG 1 cut(s) 1972
PsuI RGATCY 1 cut(s) 3305
RigI GGCCGGCC 1 cut(s) 1890
RsaI GTAC 6 cut(s) 1071, 2016, 2232, 2500, 3107, 3473
RsaNI GTAC 6 cut(s) 1070, 2015, 2231, 2499, 3106, 3472
RseI CAYNNNNRTG 3 cut(s) 266, 522, 3101
SacI GAGCTC 1 cut(s) 2089
SapI GCTCTTC 2 cut(s) 460, 3535
Sau3AI GATC 9 cut(s) 36, 681, 817, 2005, 2847, 3088, 3305, 3479, 3489
Sau96I GGNCC 2 cut(s) 271, 272
SchI GAGTC 9 cut(s) 458, 580, 710, 914, 1869, 2132, 2344, 2431, 2951
ScrFI CCNGG 7 cut(s) 276, 396, 521, 1941, 2328, 2563, 2772
SduI GDGCHC 2 cut(s) 275, 2089
SexAI ACCWGGT 1 cut(s) 2561
SfaNI GCATC 9 cut(s) 62, 142, 214, 304, 324, 860, 2239, 2792, 2970
SfcI CTRYAG 1 cut(s) 3037
SfuI TTCGAA 2 cut(s) 1875, 3160
SmiMI CAYNNNNRTG 3 cut(s) 266, 522, 3101
SmlI CTYRAG 5 cut(s) 206, 2120, 2432, 2599, 3407
SmoI CTYRAG 5 cut(s) 206, 2120, 2432, 2599, 3407
SsiI CCGC 6 cut(s) 77, 616, 892, 1543, 2677, 3432
SspI AATATT 1 cut(s) 2050
SspMI CTAG 6 cut(s) 432, 1325, 2018, 2189, 2309, 2948
SstI GAGCTC 1 cut(s) 2089
StyD4I CCNGG 7 cut(s) 274, 394, 519, 1939, 2326, 2561, 2770
StyI CCWWGG 4 cut(s) 123, 2413, 3118, 3185
TaiI ACGT 4 cut(s) 12, 1417, 3053, 3230
TaqI TCGA 9 cut(s) 200, 388, 1231, 1764, 1875, 2567, 2978, 3062, 3160
TatI WGTACW 4 cut(s) 2014, 2498, 3105, 3471
TauI GCSGC 1 cut(s) 1545
TfiI GAWTC 6 cut(s) 1490, 1810, 2029, 2331, 2419, 3194
TscAI CASTG 4 cut(s) 1977, 2545, 2866, 3239
TseFI GTSAC 5 cut(s) 515, 737, 2464, 2943, 2957
Tsp45I GTSAC 5 cut(s) 515, 737, 2464, 2943, 2957
TspGWI ACGGA 2 cut(s) 1483, 2466
TspRI CASTG 4 cut(s) 1977, 2545, 2866, 3239
Van91I CCANNNNNTGG 3 cut(s) 2414, 2886, 3119
VspI ATTAAT 1 cut(s) 3246
XapI RAATTY 7 cut(s) 1182, 1274, 1536, 2023, 2268, 2296, 3259
XbaI TCTAGA 1 cut(s) 1324
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 3421
XmnI GAANNNNTTC 2 cut(s) 2403, 2745
XspI CTAG 6 cut(s) 432, 1325, 2018, 2189, 2309, 2948
ZraI GACGTC 1 cut(s) 3051
Zsp2I ATGCAT 1 cut(s) 2590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.