pycom05g04280

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000400
Physical Location & Seq
Forward (+)
5023029 .. 5026715
3687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04280.1

Sequence Viewer

Length: 3573 bp
ATGCTATCCTTGCTTAACATCTGGTTTTCTTTGCCCCTGGATATGGATGCCACTGTAGGCATCATTTTGTCCCCGGCTCTGCAGGTTCTTTTTGACAGACTGGCATCCCCAGTCCTACAAGGGCTTGCTGATATCCTGGGTTTCAATTTTGACATCTTCCAAAGTTTGCAGCATGCACTGGTGCGGGCTCAAGCTACTCTTGAAGATGCAGAAGTGCAACAATTCACCAACAAAACTGTCAGACTCTGGCTGGCTGATCTCAAGAATACAGTTTGTGATGCTGAGGATCTTCTCGACGTCTTTACTGTTAAACAAACTGCCATGATTGACCAAGACTTTGGTAAGCAGACAGTAGAAAGCTATACAGTTCTTACCGATAAAGTCAGGAAAATACTCCAGAAGCTAGAAGTGATTGTAGCTGAAGGATCTTCTAAGTTGAAGATTAGAGAGCCTACTCAGCCAAGGTCAGATCGACAATCGGACAAAAGGGAGACTAGCTCTTTCATCGACTCAAGGATATATGGAAGAGATGATGACAAAGAGACGTTAGTCCAACTGTTGATGTCTTCTTATATTGTTTACCATGAGGGATATACATATGCATCGTGTATTCCCATCATCGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAATTAGCATACAATGATAAGGTGATTATTCAACACTTTGATGTTAAGATGTGGATTTTTGTCTCTTTTGATTTCAATGTCAAGAAGATCATGAAGACAATTATTGCGTCTATAACGAATGATGAATGCAAGTTGTCGGAGATTGAACTACTTCAGTCTCGAATTTGGCAATTATTGCAGAACAAAAGATATCTTATCGTTTTGGATGATGTTTGGACTGAAGACCAGGATGATTGGGACAAACTAAGACCCTTGTTTAGAGAGGGTGTTGATGGATGCAAAATCATTGTCACTACCCGCAGTAAAAAGATCCCATTCATGATGGACTTCCCAAATTCCCCAATGTATTTGAGTGGTTTGACGGATGATGACTGTTGGGCTTTGTTCAAGCAACGCGCTTTTGGACGAGGAGAAGAAGAGAATTATCTGAACCTTTCGCTGATTGGGAAACAGATTGTCAGAAAATGTGGAGGTGTGCCATTAGCAGCAAATAGTTTGGGAAGTTCAATGCGCTTGAAAAGAGACGAAAAGCAATGGTTGTCTATGCGAGATTGCGAACTTTGGAAATTAGATGAAAAGGAGCATAAAGTTTTGCCCGCCCTCATGCTGAGCTATCATCATCTACCATCACATCTCAGAGAATGCTTTTCGTTTTGCTCAATTTTTCCAAAAAATTATGAATTCAAGAAGGAGAAATTAATTCATATGTGGATGGCATCAAGCTTAATTCTACAAGATGGGAGCCGGCGGCCAGAGGACATTGCTGATGAATACTTTGCCGGTTTGTTGTGGTTGTCTTTCTTTCAAGAAGTAAAGGGTGATGGTGGGGCCTTAGTTGGATACAAGATGAATGATGTTATTCATGATCTTGCACAATATGTAGCAGGAAACGAATCTTTGATGCTTGAACATAGTGCAGCACAAATTCGCCATGCATCTGTTGTTTACAAGTACAAGGCAATTGGAATTCCAAAGGACCTACTTGAAGCAAAACATTTACGAACTCTCTTATTGATTGGGGAATCCGGCTTGTTAAATAACAGAAGTAAAATGTTTTCAAGTTTTGGGTACTTACGTCTACTAGACCTGAGCAGTTGTGGTGTTTCTGATTTGCCAGAATCATTAGGTGGCTTGATATGCTTGAGGTATCTTGACCTATCATACACACCTATTTTTGAGTTACCTCACAGCACAAGGAATCTCTGCTCTTTGCAGACTTTGAACCTATTTGGTTGTCGGAATCTTATACGGTTGCCAAGCTTGGTAAAGATGATCAGCCTAAGACATCTCAATCTAATTGGATGTGTAAGTTTGGCTTCCATGCCTCTTGAGATTGGAAAATTACGGAAACTTCAAACACTGCCATTGTTTGTGGTTAACAGGATTCCAATGGCCCTTGGTGCGCTGAAACGTTTAAATCTTTATGGAAAGTTGAATGTTACCCGCTTGGAGAATGCAAGATATGGAGCCGATGCAGAGTCAGCTGGATTGAAGTTGAAAGAAAATCTTGAGTCATTAGGGTTATATTGGGGACCATGTAGTGGATTTGAAGACAGTCAAGAATCATTTGGGAAACCTGAAGCCCGACATGAAGAATTTTCTTTCGGATATCAGACCGTGACAGGACAACGTGACACTATGGAAAAAATTCTTGAAGGCCTGGAACCTCACCAAAATCTGAAAAAGCTGATTATAAATGGCTACCCAGGAATCAGATTTCCTCAGTGGGCTCTCCCAAATCTTGTTGCCATCAATTTCACCAACTGTACAAATTGTGAGCATCTTCCTGCCCTTGGGAATCTTCTGCTACTCAAGACTCTTTCTCTGCATAGAATGCATGGTGTGAAGAGAATTGGTGTAGAGTTATATGGTGATGGTATGGACGTATGGTTTCCATCACTCGAAGAACTATTAATAAGTGATTTTCCCAACTTGGAAGAGTGGTCAAATGCAAATGGTGGAAGTGCATTCTCTAGATTGAAGAAATTAACTGTCAAAAGGTGTCCCAAGTTAGCACATATGCCATTACCTCAGTTCCTTGAGCATTTGGAGCTACGGGACTGTAATCCAACGATGACGTCCACATCAAGTTTAAGTTTGCTTTCTGTGCTTGTTCTAGAAAAAATTCCAAACTTGTTCTCTTTGCCAGAAGGGCTCTTTGCATCAGCTAGTCTGTCTTCTTTAAAGATCTTATCGTGCCCCAAGCTTCGTTCAGTGCCTTTGGAGATTGGAACCCTCACTGCTTTGAAATCACTGACCATTAGTTGGTGTGATGAGTTGTCCTATCTGCCACAGAGTTTGCAGAACCTCAAGAGTCTGGAGTCACTGGAGATTAGTGACTGTCATAGTCTAATATCCATGCCAAATGGTGGAATTGCAAGTCTAAGTTCTCTTCGAACTTTGTCCATTGAGAACTGCAGCAATCTGACTTTTTTGTCATCGAGTTTGGAGCATCTCAAATTTCTCGAGCACTTGACAATAATGTATTGTCCAAAGCTTGGTTCTTTTCCAGAGGGCGTGCAACACCTCTCTTCACTTCGAAGTTTCACTATCTTGAACTGTCCTTGGTTTGATACTCTACCTATTGGATTAATGAATCTTCAAACGCTGCACTGCTTGGAAATTAGTAGCTGCCCTAACCTAGATGCTTTGCCAGACTGGTTGGAGAATCTTGCTTCACTTAGATCACTAACCATATCAGACTGTCCTAATTCAAGAGTTCTGCCACCAGGCCTCAAGTATCTCAAAGAACTCCAACACCTCTCCATTCAAGAATGTCCCGAGCTAGAGGAAAGATGTAAACAAGGTAGTGGTGAGGACTGGTTGAAAATAGCTCATGTCCCACACAAGTACATTGGCTCACCTGATCAAGCCATGCAGTCTGACAAAGCAAGCACATCCAGCAGCTCTTCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1191

Amino Acids

134.12

Weight (kDa)

6.22

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 23 - 106 2.8e-14 Rx N-terminal domain
NB-ARC PF00931 203 - 353 7e-38 NB-ARC domain
WHD_DRP PF23559 439 - 509 7e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 568 - 858 6.3e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 685 - 830 6.6e-28 R13L1/DRL21 LRRs
LRR_14 PF23598 908 - 1046 3.9e-09 Leucine-rich repeat region
LRR_14 PF23598 1034 - 1150 1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2348
AasI GACNNNNNNGTC 1 cut(s) 3088
AatII GACGTC 2 cut(s) 300, 2735
Acc36I ACCTGC 1 cut(s) 73
AccB7I CCANNNNNTGG 4 cut(s) 2195, 2919, 3023, 3152
AccI GTMKAC 1 cut(s) 1732
AccII CGCG 1 cut(s) 1050
AciI CCGC 5 cut(s) 184, 952, 1251, 1402, 2098
AclI AACGTT 1 cut(s) 2065
AclWI GGATC 3 cut(s) 294, 433, 958
AcoI YGGCCR 1 cut(s) 1403
AcsI RAATTY 9 cut(s) 816, 988, 1334, 1578, 1620, 2249, 2301, 2778, 3113
AcuI CTGAAG 4 cut(s) 441, 791, 894, 2253
AcyI GRCGYC 2 cut(s) 297, 2732
AfaI GTAC 4 cut(s) 1607, 1724, 2422, 3506
AfiI CCNNNNNNNGG 7 cut(s) 43, 620, 2195, 2447, 2919, 3023, 3152
AjnI CCWGG 6 cut(s) 36, 135, 879, 2313, 2359, 3382
AjuI GAANNNNNNNTTGG 2 cut(s) 3139, 3171
Alw21I GWGCWC 1 cut(s) 3126
Alw26I GTCTC 5 cut(s) 485, 536, 721, 816, 1170
AlwI GGATC 3 cut(s) 294, 433, 958
AlwNI CAGNNNCTG 1 cut(s) 246
Ama87I CYCGRG 2 cut(s) 3119, 3434
AoxI GGCC 5 cut(s) 1403, 1482, 2046, 2311, 3385
ApeKI GCWGC 7 cut(s) 169, 1139, 1571, 3072, 3262, 3285, 3558
ApoI RAATTY 9 cut(s) 816, 988, 1334, 1578, 1620, 2249, 2301, 2778, 3113
ArsI GACNNNNNNTTYG 4 cut(s) 320, 352, 927, 959
AseI ATTAAT 3 cut(s) 1352, 2567, 3245
Asp700I GAANNNNTTC 3 cut(s) 804, 2301, 2778
AspLEI GCGC 3 cut(s) 1052, 1167, 2059
AspS9I GGNCC 4 cut(s) 1482, 1630, 2047, 2186
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 8 cut(s) 217, 688, 1484, 2315, 2404, 2537, 3479, 3507
AsuII TTCGAA 2 cut(s) 3049, 3193
AvaI CYCGRG 2 cut(s) 3119, 3434
AvaII GGWCC 2 cut(s) 1630, 2186
BaeGI GKGCMC 1 cut(s) 2855
BaeI ACNNNNGTAYC 2 cut(s) 1714, 1747
BanII GRGCYC 3 cut(s) 190, 2386, 2811
BbsI GAAGAC 5 cut(s) 558, 755, 882, 2211, 2823
Bbv12I GWGCWC 1 cut(s) 3126
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 6 cut(s) 181, 1151, 1583, 3084, 3249, 3272
BciT130I CCWGG 6 cut(s) 38, 137, 881, 2315, 2361, 3384
BciVI GTATCC 1 cut(s) 1487
BclI TGATCA 2 cut(s) 1926, 3520
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 5 cut(s) 485, 536, 721, 816, 1170
BfaI CTAG 8 cut(s) 404, 495, 1736, 2628, 2771, 2823, 3296, 3440
BfmI CTRYAG 3 cut(s) 54, 80, 3070
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 1487
BglI GCCNNNNNGGC 1 cut(s) 2806
BglII AGATCT 1 cut(s) 2841
BisI GCNGC 8 cut(s) 170, 1140, 1403, 1572, 3073, 3263, 3286, 3559
BlpI GCTNAGC 1 cut(s) 1262
BlsI GCNGC 8 cut(s) 171, 1141, 1404, 1573, 3074, 3264, 3287, 3560
Bme1390I CCNGG 7 cut(s) 38, 74, 137, 881, 2315, 2361, 3384
Bme18I GGWCC 2 cut(s) 1630, 2186
BmeT110I CYCGRG 2 cut(s) 3119, 3434
BmgT120I GGNCC 4 cut(s) 1482, 1630, 2047, 2186
BmiI GGNNCC 6 cut(s) 1397, 1483, 2122, 2187, 2319, 2887
BmrFI CCNGG 7 cut(s) 38, 74, 137, 881, 2315, 2361, 3384
BmrI ACTGGG 1 cut(s) 104
BmuI ACTGGG 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 548
BpiI GAAGAC 5 cut(s) 558, 755, 882, 2211, 2823
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 3 cut(s) 380, 2993, 3002
Bpu10I CCTNAGC 2 cut(s) 282, 1742
Bpu1102I GCTNAGC 1 cut(s) 1262
Bpu14I TTCGAA 2 cut(s) 3049, 3193
BpuMI CCSGG 1 cut(s) 74
BsaHI GRCGYC 2 cut(s) 297, 2732
BsaJI CCNNGG 8 cut(s) 36, 72, 136, 461, 2050, 2359, 2446, 3218
Bsc4I CCNNNNNNNGG 7 cut(s) 43, 620, 2195, 2447, 2919, 3023, 3152
Bse118I RCCGGY 2 cut(s) 1398, 1433
Bse1I ACTGG 6 cut(s) 105, 110, 183, 2985, 3317, 3479
Bse3DI GCAATG 2 cut(s) 1193, 1413
BseBI CCWGG 6 cut(s) 38, 137, 881, 2315, 2361, 3384
BseDI CCNNGG 8 cut(s) 36, 72, 136, 461, 2050, 2359, 2446, 3218
BseGI GGATG 9 cut(s) 52, 104, 865, 889, 935, 1024, 1371, 1961, 3551
BseLI CCNNNNNNNGG 7 cut(s) 43, 620, 2195, 2447, 2919, 3023, 3152
BseMI GCAATG 2 cut(s) 1193, 1413
BseMII CTCAG 7 cut(s) 273, 470, 1253, 1303, 1733, 2390, 2699
BseNI ACTGG 6 cut(s) 105, 110, 183, 2985, 3317, 3479
BseRI GAGGAG 1 cut(s) 1077
BseSI GKGCMC 1 cut(s) 2855
BseXI GCAGC 6 cut(s) 181, 1151, 1583, 3084, 3249, 3272
BsgI GTGCAG 2 cut(s) 1590, 3248
Bsh1236I CGCG 1 cut(s) 1050
BshFI GGCC 5 cut(s) 1405, 1484, 2048, 2313, 3387
BsiHKAI GWGCWC 1 cut(s) 3126
BsiHKCI CYCGRG 2 cut(s) 3119, 3434
BsiSI CCGG 4 cut(s) 74, 1399, 1434, 1680
BslFI GGGAC 7 cut(s) 55, 905, 2199, 2643, 2726, 3417, 3479
BslI CCNNNNNNNGG 7 cut(s) 43, 620, 2195, 2447, 2919, 3023, 3152
BsmAI GTCTC 5 cut(s) 485, 536, 721, 816, 1170
BsmBI CGTCTC 2 cut(s) 536, 1170
BsmFI GGGAC 7 cut(s) 55, 905, 2199, 2643, 2726, 3417, 3479
BsmI GAATGC 5 cut(s) 785, 1301, 2113, 2493, 2621
BsnI GGCC 5 cut(s) 1405, 1484, 2048, 2313, 3387
BsoBI CYCGRG 2 cut(s) 3119, 3434
Bsp119I TTCGAA 2 cut(s) 3049, 3193
Bsp1286I GDGCHC 5 cut(s) 190, 2386, 2811, 2855, 3126
Bsp1407I TGTACA 1 cut(s) 2420
Bsp1720I GCTNAGC 1 cut(s) 1262
BspACI CCGC 5 cut(s) 184, 952, 1251, 1402, 2098
BspANI GGCC 5 cut(s) 1405, 1484, 2048, 2313, 3387
BspCNI CTCAG 7 cut(s) 274, 469, 1254, 1302, 1734, 2389, 2698
BspFNI CGCG 1 cut(s) 1050
BspHI TCATGA 3 cut(s) 744, 972, 1516
BspLI GGNNCC 6 cut(s) 1397, 1483, 2122, 2187, 2319, 2887
BspMAI CTGCAG 2 cut(s) 84, 3074
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 3 cut(s) 294, 433, 958
BspQI GCTCTTC 1 cut(s) 3568
BspT104I TTCGAA 2 cut(s) 3049, 3193
BsrDI GCAATG 2 cut(s) 1193, 1413
BsrFI RCCGGY 2 cut(s) 1398, 1433
BsrGI TGTACA 1 cut(s) 2420
BsrI ACTGG 6 cut(s) 105, 110, 183, 2985, 3317, 3479
BssAI RCCGGY 2 cut(s) 1398, 1433
BssECI CCNNGG 8 cut(s) 36, 72, 136, 461, 2050, 2359, 2446, 3218
BssNI GRCGYC 2 cut(s) 297, 2732
BssT1I CCWWGG 4 cut(s) 461, 2050, 2446, 3218
Bst2UI CCWGG 6 cut(s) 38, 137, 881, 2315, 2361, 3384
Bst6I CTCTTC 7 cut(s) 520, 1065, 2495, 2586, 3051, 3190, 3568
BstACI GRCGYC 2 cut(s) 297, 2732
BstAPI GCANNNNNTGC 2 cut(s) 829, 2488
BstAUI TGTACA 1 cut(s) 2420
BstBI TTCGAA 2 cut(s) 3049, 3193
BstC8I GCNNGC 8 cut(s) 126, 174, 186, 252, 1251, 1400, 3173, 3547
BstF5I GGATG 9 cut(s) 52, 104, 865, 889, 935, 1024, 1371, 1961, 3551
BstFNI CGCG 1 cut(s) 1050
BstHHI GCGC 3 cut(s) 1052, 1167, 2059
BstMAI GTCTC 5 cut(s) 485, 536, 721, 816, 1170
BstNI CCWGG 6 cut(s) 38, 137, 881, 2315, 2361, 3384
BstNSI RCATGY 1 cut(s) 176
BstPAI GACNNNNGTC 1 cut(s) 548
BstSCI CCNGG 7 cut(s) 36, 72, 135, 879, 2313, 2359, 3382
BstSFI CTRYAG 3 cut(s) 54, 80, 3070
BstSLI GKGCMC 1 cut(s) 2855
BstUI CGCG 1 cut(s) 1050
BstV1I GCAGC 6 cut(s) 181, 1151, 1583, 3084, 3249, 3272
BstV2I GAAGAC 5 cut(s) 558, 755, 882, 2211, 2823
BstX2I RGATCY 4 cut(s) 286, 425, 963, 2841
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 4 cut(s) 286, 425, 963, 2841
BsuI GTATCC 1 cut(s) 1487
BsuRI GGCC 5 cut(s) 1405, 1484, 2048, 2313, 3387
BtsCI GGATG 9 cut(s) 52, 104, 865, 889, 935, 1024, 1371, 1961, 3551
BtsI GCAGTG 3 cut(s) 2012, 2892, 3265
BtsIMutI CAGTG 9 cut(s) 51, 176, 2012, 2384, 2874, 2892, 2906, 2978, 3265
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 8 cut(s) 126, 174, 186, 252, 1251, 1400, 3173, 3547
CaiI CAGNNNCTG 1 cut(s) 246
CciI TCATGA 3 cut(s) 744, 972, 1516
CfoI GCGC 3 cut(s) 1052, 1167, 2059
Cfr10I RCCGGY 2 cut(s) 1398, 1433
Cfr13I GGNCC 4 cut(s) 1482, 1630, 2047, 2186
CseI GACGC 1 cut(s) 750
Csp6I GTAC 4 cut(s) 1606, 1723, 2421, 3505
CspCI CAANNNNNGTGG 2 cut(s) 2934, 2969
CviQI GTAC 4 cut(s) 1606, 1723, 2421, 3505
DraI TTTAAA 2 cut(s) 2070, 2838
DrdI GACNNNNNNGTC 1 cut(s) 3088
DseDI GACNNNNNNGTC 1 cut(s) 3088
EaeI YGGCCR 1 cut(s) 1403
Eam1104I CTCTTC 7 cut(s) 520, 1065, 2495, 2586, 3051, 3190, 3568
EarI CTCTTC 7 cut(s) 520, 1065, 2495, 2586, 3051, 3190, 3568
Eco130I CCWWGG 4 cut(s) 461, 2050, 2446, 3218
Eco147I AGGCCT 2 cut(s) 2313, 3387
Eco24I GRGCYC 3 cut(s) 190, 2386, 2811
Eco32I GATATC 3 cut(s) 133, 845, 2264
Eco47I GGWCC 2 cut(s) 1630, 2186
Eco57I CTGAAG 4 cut(s) 441, 791, 894, 2253
Eco88I CYCGRG 2 cut(s) 3119, 3434
EcoO109I RGGNCCY 2 cut(s) 1482, 1630
EcoRI GAATTC 2 cut(s) 1334, 1620
EcoRII CCWGG 6 cut(s) 36, 135, 879, 2313, 2359, 3382
EcoRV GATATC 3 cut(s) 133, 845, 2264
EcoT14I CCWWGG 4 cut(s) 461, 2050, 2446, 3218
EcoT22I ATGCAT 3 cut(s) 604, 1591, 2493
EcoT38I GRGCYC 3 cut(s) 190, 2386, 2811
ErhI CCWWGG 4 cut(s) 461, 2050, 2446, 3218
Esp3I CGTCTC 2 cut(s) 536, 1170
FalI AAGNNNNNCTT 6 cut(s) 183, 215, 1954, 1986, 2145, 2177
FaqI GGGAC 7 cut(s) 55, 905, 2199, 2643, 2726, 3417, 3479
FauI CCCGC 4 cut(s) 177, 959, 1258, 2105
FauNDI CATATG 3 cut(s) 598, 1359, 2673
FbaI TGATCA 2 cut(s) 1926, 3520
FblI GTMKAC 1 cut(s) 1732
Fnu4HI GCNGC 8 cut(s) 170, 1140, 1403, 1572, 3073, 3263, 3286, 3559
FokI GGATG 9 cut(s) 59, 91, 872, 896, 942, 1031, 1378, 1968, 3538
FriOI GRGCYC 3 cut(s) 190, 2386, 2811
Fsp4HI GCNGC 8 cut(s) 170, 1140, 1403, 1572, 3073, 3263, 3286, 3559
FspBI CTAG 8 cut(s) 404, 495, 1736, 2628, 2771, 2823, 3296, 3440
GlaI GCGC 3 cut(s) 1051, 1166, 2058
GluI GCNGC 8 cut(s) 170, 1140, 1403, 1572, 3073, 3263, 3286, 3559
GsuI CTGGAG 3 cut(s) 380, 2993, 3002
HaeIII GGCC 5 cut(s) 1405, 1484, 2048, 2313, 3387
HapII CCGG 4 cut(s) 74, 1399, 1434, 1680
HgaI GACGC 1 cut(s) 750
HhaI GCGC 3 cut(s) 1052, 1167, 2059
Hin1I GRCGYC 2 cut(s) 297, 2732
Hin6I GCGC 3 cut(s) 1050, 1165, 2057
HinP1I GCGC 3 cut(s) 1050, 1165, 2057
HincII GTYRAC 1 cut(s) 2032
HindII GTYRAC 1 cut(s) 2032
HindIII AAGCTT 4 cut(s) 1375, 1912, 2858, 3149
HpaI GTTAAC 1 cut(s) 2032
HpaII CCGG 4 cut(s) 74, 1399, 1434, 1680
HphI GGTGA 8 cut(s) 217, 688, 1484, 2315, 2404, 2537, 3479, 3507
Hpy166II GTNNAC 6 cut(s) 580, 1600, 1733, 2032, 2736, 3455
Hpy8I GTNNAC 6 cut(s) 580, 1600, 1733, 2032, 2736, 3455
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 4 cut(s) 416, 1336, 2303, 2798
HpyCH4IV ACGT 7 cut(s) 297, 545, 1729, 2065, 2284, 2538, 2732
HpySE526I ACGT 7 cut(s) 297, 545, 1729, 2065, 2284, 2538, 2732
Hsp92I GRCGYC 2 cut(s) 297, 2732
HspAI GCGC 3 cut(s) 1050, 1165, 2057
KroI GCCGGC 1 cut(s) 1398
KroNI GCCGGC 1 cut(s) 1400
Ksp22I TGATCA 2 cut(s) 1926, 3520
KspAI GTTAAC 1 cut(s) 2032
LguI GCTCTTC 1 cut(s) 3568
LmnI GCTCC 5 cut(s) 1234, 1395, 2120, 2704, 3103
Lsp1109I GCAGC 6 cut(s) 181, 1151, 1583, 3084, 3249, 3272
MaeI CTAG 8 cut(s) 404, 495, 1736, 2628, 2771, 2823, 3296, 3440
MaeII ACGT 7 cut(s) 297, 545, 1729, 2065, 2284, 2538, 2732
MaeIII GTNAC 7 cut(s) 943, 1833, 2092, 2272, 2285, 2976, 2990
MfeI CAATTG 1 cut(s) 1614
MflI RGATCY 4 cut(s) 286, 425, 963, 2841
MhlI GDGCHC 5 cut(s) 190, 2386, 2811, 2855, 3126
MlyI GAGTC 7 cut(s) 237, 503, 2141, 2174, 2464, 2977, 2984
MmeI TCCRAC 7 cut(s) 577, 771, 1471, 1871, 2747, 3297, 3433
Mph1103I ATGCAT 3 cut(s) 604, 1591, 2493
MroNI GCCGGC 1 cut(s) 1398
MroXI GAANNNNTTC 3 cut(s) 804, 2301, 2778
MslI CAYNNNNRTG 1 cut(s) 693
MspA1I CMGCKG 1 cut(s) 2138
MspI CCGG 4 cut(s) 74, 1399, 1434, 1680
MspR9I CCNGG 7 cut(s) 38, 74, 137, 881, 2315, 2361, 3384
MunI CAATTG 1 cut(s) 1614
Mva1269I GAATGC 5 cut(s) 785, 1301, 2113, 2493, 2621
MvaI CCWGG 6 cut(s) 38, 137, 881, 2315, 2361, 3384
MvnI CGCG 1 cut(s) 1050
NaeI GCCGGC 1 cut(s) 1400
NciI CCSGG 1 cut(s) 74
NdeI CATATG 3 cut(s) 598, 1359, 2673
NgoMIV GCCGGC 1 cut(s) 1398
NlaIV GGNNCC 6 cut(s) 1397, 1483, 2122, 2187, 2319, 2887
NmuCI GTSAC 5 cut(s) 943, 2272, 2285, 2976, 2990
NsiI ATGCAT 3 cut(s) 604, 1591, 2493
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 2 cut(s) 3049, 3193
PaeI GCATGC 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 3119
PagI TCATGA 3 cut(s) 744, 972, 1516
PceI AGGCCT 2 cut(s) 2313, 3387
PciSI GCTCTTC 1 cut(s) 3568
PctI GAATGC 5 cut(s) 785, 1301, 2113, 2493, 2621
PdiI GCCGGC 1 cut(s) 1400
PdmI GAANNNNTTC 3 cut(s) 804, 2301, 2778
PflMI CCANNNNNTGG 4 cut(s) 2195, 2919, 3023, 3152
PkrI GCNGC 8 cut(s) 171, 1141, 1404, 1573, 3074, 3264, 3287, 3560
PleI GAGTC 7 cut(s) 237, 503, 2140, 2173, 2464, 2976, 2983
PpsI GAGTC 7 cut(s) 237, 503, 2140, 2173, 2464, 2976, 2983
PpuMI RGGWCCY 1 cut(s) 1630
PshAI GACNNNNGTC 1 cut(s) 548
PshBI ATTAAT 3 cut(s) 1352, 2567, 3245
PsiI TTATAA 1 cut(s) 2348
Psp1406I AACGTT 1 cut(s) 2065
Psp5II RGGWCCY 1 cut(s) 1630
Psp6I CCWGG 6 cut(s) 36, 135, 879, 2313, 2359, 3382
PspGI CCWGG 6 cut(s) 36, 135, 879, 2313, 2359, 3382
PspN4I GGNNCC 6 cut(s) 1397, 1483, 2122, 2187, 2319, 2887
PspPI GGNCC 4 cut(s) 1482, 1630, 2047, 2186
PspPPI RGGWCCY 1 cut(s) 1630
PstI CTGCAG 2 cut(s) 84, 3074
PstNI CAGNNNCTG 1 cut(s) 246
PsuI RGATCY 4 cut(s) 286, 425, 963, 2841
PvuII CAGCTG 1 cut(s) 2138
RsaI GTAC 4 cut(s) 1607, 1724, 2422, 3506
RsaNI GTAC 4 cut(s) 1606, 1723, 2421, 3505
RseI CAYNNNNRTG 1 cut(s) 693
SapI GCTCTTC 1 cut(s) 3568
SatI GCNGC 8 cut(s) 170, 1140, 1403, 1572, 3073, 3263, 3286, 3559
Sau96I GGNCC 4 cut(s) 1482, 1630, 2047, 2186
SchI GAGTC 7 cut(s) 237, 503, 2141, 2174, 2464, 2977, 2984
ScrFI CCNGG 7 cut(s) 38, 74, 137, 881, 2315, 2361, 3384
SduI GDGCHC 5 cut(s) 190, 2386, 2811, 2855, 3126
SfcI CTRYAG 3 cut(s) 54, 80, 3070
Sfr274I CTCGAG 1 cut(s) 3119
SfuI TTCGAA 2 cut(s) 3049, 3193
SinI GGWCC 2 cut(s) 1630, 2186
SlaI CTCGAG 1 cut(s) 3119
SmiMI CAYNNNNRTG 1 cut(s) 693
SphI GCATGC 1 cut(s) 176
SseBI AGGCCT 2 cut(s) 2313, 3387
SsiI CCGC 5 cut(s) 184, 952, 1251, 1402, 2098
SspMI CTAG 8 cut(s) 404, 495, 1736, 2628, 2771, 2823, 3296, 3440
StuI AGGCCT 2 cut(s) 2313, 3387
StyD4I CCNGG 7 cut(s) 36, 72, 135, 879, 2313, 2359, 3382
StyI CCWWGG 4 cut(s) 461, 2050, 2446, 3218
TaiI ACGT 7 cut(s) 300, 548, 1732, 2068, 2287, 2541, 2735
TaqI TCGA 9 cut(s) 294, 472, 507, 814, 2556, 3049, 3095, 3120, 3193
TatI WGTACW 3 cut(s) 1605, 2420, 3504
TauI GCSGC 1 cut(s) 1405
TscAI CASTG 9 cut(s) 58, 183, 2019, 2384, 2874, 2899, 2913, 2985, 3272
TseFI GTSAC 5 cut(s) 943, 2272, 2285, 2976, 2990
TseI GCWGC 7 cut(s) 169, 1139, 1571, 3072, 3262, 3285, 3558
Tsp45I GTSAC 5 cut(s) 943, 2272, 2285, 2976, 2990
TspGWI ACGGA 2 cut(s) 1031, 2014
TspRI CASTG 9 cut(s) 58, 183, 2019, 2384, 2874, 2899, 2913, 2985, 3272
Van91I CCANNNNNTGG 4 cut(s) 2195, 2919, 3023, 3152
VpaK11BI GGWCC 2 cut(s) 1630, 2186
VspI ATTAAT 3 cut(s) 1352, 2567, 3245
XapI RAATTY 9 cut(s) 816, 988, 1334, 1578, 1620, 2249, 2301, 2778, 3113
XbaI TCTAGA 2 cut(s) 2627, 2770
XceI RCATGY 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 3119
XmiI GTMKAC 1 cut(s) 1732
XmnI GAANNNNTTC 3 cut(s) 804, 2301, 2778
XspI CTAG 8 cut(s) 404, 495, 1736, 2628, 2771, 2823, 3296, 3440
ZraI GACGTC 2 cut(s) 298, 2733
Zsp2I ATGCAT 3 cut(s) 604, 1591, 2493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.