MD05G1050800.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000400
Physical Location & Seq
Forward (+)
8810208 .. 8813238
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050800.v1.1.491

Sequence Viewer

Length: 2991 bp
ATGGATGCCGCTGTAGGCATCATTTTGTCCCCGGCTCTGCAGGTTCTTTTCGACAGACTGGCATCCCCAGTCCTACAAGGGCTTGCTGATATCCTGGGTTTCAATTTTGGCATCTTCCAAAGCCTGCAGCATGCACTGGTGCGGGCTCAAGCTACTCTTGAAGATGCAGAAGTGCAACAATTCACCAACAGAACTGTCAGACTCTGGCTGGCGGATCTCAAGAATGCAGTGTGTGATGCTGAGGATCTTCTTGACGTCTTTACTGTTAAACAAACTGCCATGATTGACCAAGACTTTGGCAAGCAGACAGTAGAAAGCTATACAGTTCTTACTGATAAAGTCAGGAAGATACTCCAGAAGCTAGAAGTGACTGTAGCTGAAGGATCTTCTAAGTTGAAGATTAGAGAGCCTACTCAGCCAAGGTCAGATCGACAATCGGACAAAAGGGAGACTAGCTCTTTCATCGACTCGAGGATATATGGAAGAGATGATGACAAAGAGACGTTAGTCCAACTGTTGATGTCTTCTCAAACTGTTTACCAGGAGGGATATACGTATGCATCGTGTATTCCCATCATCGGTATTGGAGGAATTGGTAAGACCACTCTTGCTCAATTAGCATACAATGATAAGGTGGTTATTCAACACTTCGATATTAGGATGTGGATTTTTGTCTCTTCTGATTTCAATGTCAAGAAGATCATGAAGACAATTATTGCGTCTATAACGAATGATGAATGCAAGTTGTCGGAGAATGAACTACTTCAGTCTCGGCTTTGGCAATTGCTGCAGAACAAAAGATATCTGATCGTTTTAGATGATGTTTGGACTGAAGACCAGGATGATTGGGACAAACTAAGACCTTTGTTTAGAGAGGGTGTTGATGGATGCAAAATCATTGTCACTACCCGCAGTAAAAAGATCCCATTCATGATGGACTTCCCAAATTCCCCAATGTATTTGAGTGGTTTGACGGATGATGACTGTTGGGCTTTGTTCAAGCAACGCGCTTTTGGACGAGGAGAAGAAGAGAAATATCCGAACCTTTCGCTGATTGGGAAACAGATTGTCAGAAAATGTGGAGGTGTGCCATTAGCAGCAAAAAGTTTGGGAAGTTCAATGCGCTTGAAAAGAGACGAAAAGCAATGGTTGTCTATGCGAGATTGCGAACTTTGGAAGTTAGATGAAAAGCAGCATAAAGTTTTGCCTGCCCTCATGCTGAGCTATCATAATCTACCATCACATCTCAGAGAATGCTTTTCATTTTGCTCAATTTTTCCAAAAAATTATGAATTCAAGAAGGAGAAGTTAATTCATATGTGGATGGCATCAGGCTTAATTCCACAAGATGGGAGCCGGCGGCCAGAAGACATCGGTGATGAGTACTTTGCCGGTTTGTTGTGGTTGTCTTTCTTTCAAGAAGTAGGGGGTGATGGTGGGGCCTTAGTTGGATACAAGATGAATGATGTTATTCATGATCTTGCACAATATGTAGCAGGAAACGAATCCTTGATGCTGGAACATAGTGCAGCACAGATTCGCCATGCATCTGTTGTTTACAAGTACAGAGCAATTGGAATTCCAAAGGAGCTACTTGAAGCAAAACATTTGCGAACTCTCTTATTGATTGGAGAATCCGGCTTGTTAAATAACAGAAGTAAAATGTTTTCAAGTTTTGGGTACTTGCGTCTACTAGACCTGAGCAGTTGTGGTGTTTTTGATTTGCCAGAATCATTAGGTGGCTTGATATGCTTGAGGTATCTTGACCTATCTTACACACCTATTTTTGAGTTACCTCACAGCACAAGGAATCTCTGCTCTTTGCAGACTTTGAACCTATTTGGTTGTCGGAATCTTATACGGTTGCCAAGCTTGGTAAAGATGATCAGCCTAAGACATCTCAATCTAATTGGATGTGTAAGTTTGGCTTCCATGCCTCTTGAGATTGGAAAATTACGGAAACTTCAGACACTGCCATTGTTTGTCGTCAACAGGATTCCAAAGGCCCTTAGTACGCTGGAAGGTTTAAATCTTTATGGAAAGTTGAATATTACCCGCTTGGAGAATGCAAGATATGCAGAGTCAGCTGGATTGAAGTTGAAAGAAAATCTTGAGTCATTAGGATTATATTGGGGACCACGTAGTGGATTTGAAGACGGTCAAGAATCATTTGGGAAACCTGAAGCCCAGCGTGAAGAATTTGCTTTCGGATATCAGACAGTGACAGGACAACGTGACTCTCTGGAAAAAATTCTTGAAGGCCTGGAACCTCACCAAAATCTGAAAAAGCTGATTATAGATGGCTACCCAGGAATCAGATTTCCTCAGTGGGCTCTCCCAAATCTTGTTGCCGTCAATTTCACCAACTGTACAAATTGTGAGCATCTTCCTGCCCTTGGGAATCTTCTGCTACTCAAGACTCTTTCTCTGCATAGAATGGATGCTGTGAAGAGAATTGGTGTAGAGTTATATGGTGATGGTATGGACGTATGGTTTCCATCACTCGAAGAACTATTAATAAGTGATTTTCCTAACTTGGAAGAGTGGTCAAATGCAAATGGTGGAAGCGCATTCCCTAGATTGAAGAAATTAACTGTCAAAAGGTGTCCCAAGTTGGCACATATGCCATTACCTCAGTTCCTTGAGCATTTGGAGCTGCGGGACTGTAATCCAACGATGACGTCCATATCAAGTTTAAGTTTGCTTTCTGTGCTTGTTCTAGAAAAAATTCCAAACTTGTTCTCTTTGCCAGAAGGGCTTTTTGCATCGGCTAGTCTGTCTTCTTTAAAGATCTTGTCGTGCCCCAAGCTTCGTTCAGTGCCTTTGGAGATTGGAACCCTCACTGCTTTGAAATCACTGACTATTAGTTGGTGTGATGAGTTATCCTATCTGCCACAGAGTTTGCAGAACCTCAGGAGTCTGGAGTCTCTGGAGATTAGTGACTGTCATAGTCTAATATCCATGCCAAATGGTGGAATTGCAGCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

997

Amino Acids

112.2

Weight (kDa)

6.56

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 92 3.6e-14 Rx N-terminal domain
NB-ARC PF00931 189 - 339 1.7e-39 NB-ARC domain
WHD_DRP PF23559 425 - 495 1.9e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 554 - 866 8.8e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 670 - 813 6.5e-26 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000400)

Species Orthologous Gene IDs
malus_domestica MD05G1050700.v1.1 MD05G1050800.v1.1 MD08G1110700.v1.1 MD15G1090000.v1.1 MD15G1090100.v1.1 MD15G1090300.v1.1
prunus_persica Prupe.1G541300_v2.0.a1 Prupe.1G541300_v2.0.a1 Prupe.8G077100_v2.0.a1 Prupe.8G077200_v2.0.a1 Prupe.8G077500_v2.0.a1 Prupe.8G077600_v2.0.a1 Prupe.8G211300_v2.0.a1
pyrus_communis pycom05g04280 pycom08g09220 pycom15g08440
rosa_chinensis RchiOBHm_Chr1g0348461 RchiOBHm_Chr1g0348561 RchiOBHm_Chr6g0269201 RchiOBHm_Chr6g0269411 RchiOBHm_Chr6g0269421 RchiOBHm_Chr6g0269431 RchiOBHm_Chr7g0232901 RchiOBHm_Chr7g0232931 RchiOBHm_Chr7g0232961 RchiOBHm_Chr7g0232971
rosa_laevigata RLG00000001310
rosa_multiflora Rmu_sc0000091.1_g000001 Rmu_sc0000091.1_g000002 Rmu_sc0000091.1_g000008 Rmu_sc0000743.1_g000003 Rmu_sc0000743.1_g000004 Rmu_sc0005353.1_g000002 Rmu_sc0008668.1_g000002 Rmu_sc0015938.1_g000001 Rmu_ssc0000018.1_g000023 Rmu_ssc0000291.1_g000019 Rmu_ssc0000291.1_g000020 Rmu_ssc0000291.1_g000026 Rmu_ssc0000291.1_g000027
rosa_roxburghii Rroxscaffold_3G00227800 Rroxscaffold_3G00227820 Rroxscaffold_4G00306930 Rroxscaffold_4G00306950 Rroxscaffold_7G00198730
rosa_rugosa Rorug01G0194900 Rorug01G0194900 Rorug01G0195000 Rorug01G0195100 Rorug06G0045900 Rorug06G0046100 Rorug06G0047500 Rorug06G0047500 Rorug06G0047600 Rorug06G0047700 Rorug07G0276400 Rorug07G0276600 Rorug07G0276800.1 Rorug07G0276900
rosa_samantha Rh1AG213000 Rh1AG213100 Rh1BG179500 Rh1CG197300 Rh1CG197400 Rh1DG209600 Rh6AG166200 Rh6AG168000 Rh6AG168100 Rh6AG168200 Rh6BG171700 Rh6BG171800 Rh6BG173500 Rh6CG165300 Rh6CG167500 Rh6CG167600 Rh6DG156900 Rh6DG159400 Rh6DG159500 Rh6DG159600 Rh7AG431600 Rh7AG431700 Rh7AG431800 Rh7AG432100 Rh7AG432200 Rh7BG404700 Rh7BG404800 Rh7BG404900 Rh7BG405000 Rh7CG450600 Rh7CG450700 Rh7CG450800 Rh7CG451200 Rh7DG421300
rosa_wichuraiana Rw0G004710 Rw0G010410 Rw1G018020 Rw1G018040 Rw6G014350 Rw6G014450 Rw6G014470 Rw7G035660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 258, 2684
Acc36I ACCTGC 1 cut(s) 31
AccB7I CCANNNNNTGG 3 cut(s) 1349, 2144, 2972
AccI GTMKAC 1 cut(s) 1690
AccII CGCG 1 cut(s) 1008
AciI CCGC 7 cut(s) 9, 142, 212, 910, 1360, 2056, 2659
AclWI GGATC 4 cut(s) 222, 252, 391, 916
AcoI YGGCCR 1 cut(s) 1361
AcsI RAATTY 6 cut(s) 946, 1292, 1578, 2198, 2250, 2727
AcuI CTGAAG 5 cut(s) 399, 749, 852, 1949, 2202
AcyI GRCGYC 2 cut(s) 255, 2681
AdeI CACNNNGTG 1 cut(s) 2144
AfaI GTAC 5 cut(s) 1385, 1565, 1682, 2014, 2371
AfiI CCNNNNNNNGG 5 cut(s) 578, 1349, 2144, 2396, 2972
AjnI CCWGG 5 cut(s) 93, 540, 837, 2262, 2308
Alw26I GTCTC 6 cut(s) 443, 494, 679, 774, 1128, 2931
AlwI GGATC 4 cut(s) 222, 252, 391, 916
AlwNI CAGNNNCTG 2 cut(s) 204, 1972
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 4 cut(s) 1361, 1440, 2004, 2260
ApeKI GCWGC 7 cut(s) 127, 787, 1097, 1192, 1529, 2656, 2981
ApoI RAATTY 6 cut(s) 946, 1292, 1578, 2198, 2250, 2727
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 885, 917
AseI ATTAAT 1 cut(s) 2516
Asp700I GAANNNNTTC 3 cut(s) 762, 2250, 2727
AspLEI GCGC 3 cut(s) 1010, 1125, 2570
AspS9I GGNCC 3 cut(s) 1440, 2005, 2135
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 6 cut(s) 175, 1388, 1442, 2264, 2353, 2486
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 1 cut(s) 2135
AxyI CCTNAGG 1 cut(s) 2912
BaeGI GKGCMC 1 cut(s) 2804
BaeI ACNNNNGTAYC 2 cut(s) 1672, 1705
BanII GRGCYC 2 cut(s) 148, 2335
BbsI GAAGAC 6 cut(s) 516, 713, 840, 1374, 2160, 2772
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 6 cut(s) 139, 774, 1109, 1204, 1541, 2643
BceAI ACGGC 1 cut(s) 2336
BciT130I CCWGG 5 cut(s) 95, 542, 839, 2264, 2310
BciVI GTATCC 1 cut(s) 1445
BclI TGATCA 1 cut(s) 1884
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 6 cut(s) 443, 494, 679, 774, 1128, 2931
BfaI CTAG 6 cut(s) 362, 453, 1694, 2577, 2720, 2772
BfmI CTRYAG 5 cut(s) 12, 38, 125, 372, 788
BfuAI ACCTGC 1 cut(s) 31
BfuI GTATCC 1 cut(s) 1445
BglI GCCNNNNNGGC 1 cut(s) 2755
BglII AGATCT 1 cut(s) 2790
BisI GCNGC 9 cut(s) 9, 128, 788, 1098, 1193, 1361, 1530, 2657, 2982
BlpI GCTNAGC 1 cut(s) 1220
BlsI GCNGC 9 cut(s) 10, 129, 789, 1099, 1194, 1362, 1531, 2658, 2983
BmcAI AGTACT 1 cut(s) 1385
Bme1390I CCNGG 6 cut(s) 32, 95, 542, 839, 2264, 2310
Bme18I GGWCC 1 cut(s) 2135
BmeT110I CYCGRG 1 cut(s) 469
BmgT120I GGNCC 3 cut(s) 1440, 2005, 2135
BmiI GGNNCC 5 cut(s) 1355, 1441, 2136, 2268, 2836
BmrFI CCNGG 6 cut(s) 32, 95, 542, 839, 2264, 2310
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 6 cut(s) 516, 713, 840, 1374, 2160, 2772
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 3 cut(s) 338, 2942, 2951
Bpu10I CCTNAGC 2 cut(s) 240, 1700
Bpu1102I GCTNAGC 1 cut(s) 1220
BpuEI CTTGAG 7 cut(s) 132, 203, 1774, 1961, 2132, 2399, 2663
BpuMI CCSGG 1 cut(s) 32
BsaAI YACGTR 2 cut(s) 555, 2141
BsaHI GRCGYC 2 cut(s) 255, 2681
BsaJI CCNNGG 5 cut(s) 30, 94, 419, 2308, 2395
Bsc4I CCNNNNNNNGG 5 cut(s) 578, 1349, 2144, 2396, 2972
Bse118I RCCGGY 2 cut(s) 1356, 1391
Bse1I ACTGG 3 cut(s) 63, 68, 141
Bse21I CCTNAGG 1 cut(s) 2912
Bse3DI GCAATG 1 cut(s) 1151
BseBI CCWGG 5 cut(s) 95, 542, 839, 2264, 2310
BseDI CCNNGG 5 cut(s) 30, 94, 419, 2308, 2395
BseGI GGATG 9 cut(s) 10, 62, 666, 847, 893, 982, 1329, 1919, 2446
BseLI CCNNNNNNNGG 5 cut(s) 578, 1349, 2144, 2396, 2972
BseMI GCAATG 1 cut(s) 1151
BseMII CTCAG 8 cut(s) 231, 428, 1211, 1261, 1691, 2339, 2648, 2926
BseNI ACTGG 3 cut(s) 63, 68, 141
BseRI GAGGAG 1 cut(s) 1035
BseSI GKGCMC 1 cut(s) 2804
BseXI GCAGC 6 cut(s) 139, 774, 1109, 1204, 1541, 2643
BseYI CCCAGC 1 cut(s) 2187
BsgI GTGCAG 1 cut(s) 1548
Bsh1236I CGCG 1 cut(s) 1008
BshFI GGCC 4 cut(s) 1363, 1442, 2006, 2262
BsiHKCI CYCGRG 1 cut(s) 469
BsiSI CCGG 4 cut(s) 32, 1357, 1392, 1638
BslFI GGGAC 5 cut(s) 13, 863, 2148, 2592, 2675
BslI CCNNNNNNNGG 5 cut(s) 578, 1349, 2144, 2396, 2972
BsmAI GTCTC 6 cut(s) 443, 494, 679, 774, 1128, 2931
BsmBI CGTCTC 2 cut(s) 494, 1128
BsmFI GGGAC 5 cut(s) 13, 863, 2148, 2592, 2675
BsmI GAATGC 5 cut(s) 229, 743, 1259, 2071, 2570
BsnI GGCC 4 cut(s) 1363, 1442, 2006, 2262
BsoBI CYCGRG 1 cut(s) 469
Bsp1286I GDGCHC 3 cut(s) 148, 2335, 2804
Bsp1407I TGTACA 1 cut(s) 2369
Bsp1720I GCTNAGC 1 cut(s) 1220
BspACI CCGC 7 cut(s) 9, 142, 212, 910, 1360, 2056, 2659
BspANI GGCC 4 cut(s) 1363, 1442, 2006, 2262
BspCNI CTCAG 8 cut(s) 232, 427, 1212, 1260, 1692, 2338, 2647, 2925
BspFNI CGCG 1 cut(s) 1008
BspHI TCATGA 3 cut(s) 702, 930, 1474
BspLI GGNNCC 5 cut(s) 1355, 1441, 2136, 2268, 2836
BspMAI CTGCAG 3 cut(s) 42, 129, 792
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 4 cut(s) 222, 252, 391, 916
BsrDI GCAATG 1 cut(s) 1151
BsrFI RCCGGY 2 cut(s) 1356, 1391
BsrGI TGTACA 1 cut(s) 2369
BsrI ACTGG 3 cut(s) 63, 68, 141
BssAI RCCGGY 2 cut(s) 1356, 1391
BssECI CCNNGG 5 cut(s) 30, 94, 419, 2308, 2395
BssNI GRCGYC 2 cut(s) 255, 2681
BssT1I CCWWGG 2 cut(s) 419, 2395
Bst2UI CCWGG 5 cut(s) 95, 542, 839, 2264, 2310
Bst6I CTCTTC 5 cut(s) 478, 682, 1023, 2444, 2535
BstACI GRCGYC 2 cut(s) 255, 2681
BstAPI GCANNNNNTGC 2 cut(s) 787, 2075
BstAUI TGTACA 1 cut(s) 2369
BstBAI YACGTR 2 cut(s) 555, 2141
BstC8I GCNNGC 8 cut(s) 84, 125, 132, 144, 210, 302, 1209, 1358
BstF5I GGATG 9 cut(s) 10, 62, 666, 847, 893, 982, 1329, 1919, 2446
BstFNI CGCG 1 cut(s) 1008
BstHHI GCGC 3 cut(s) 1010, 1125, 2570
BstMAI GTCTC 6 cut(s) 443, 494, 679, 774, 1128, 2931
BstMWI GCNNNNNNNGC 9 cut(s) 415, 617, 787, 1749, 2075, 2084, 2653, 2710, 2755
BstNI CCWGG 5 cut(s) 95, 542, 839, 2264, 2310
BstNSI RCATGY 1 cut(s) 134
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 6 cut(s) 30, 93, 540, 837, 2262, 2308
BstSFI CTRYAG 5 cut(s) 12, 38, 125, 372, 788
BstSLI GKGCMC 1 cut(s) 2804
BstSNI TACGTA 1 cut(s) 555
BstUI CGCG 1 cut(s) 1008
BstV1I GCAGC 6 cut(s) 139, 774, 1109, 1204, 1541, 2643
BstV2I GAAGAC 6 cut(s) 516, 713, 840, 1374, 2160, 2772
BstX2I RGATCY 5 cut(s) 214, 244, 383, 921, 2790
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 5 cut(s) 214, 244, 383, 921, 2790
Bsu36I CCTNAGG 1 cut(s) 2912
BsuI GTATCC 1 cut(s) 1445
BsuRI GGCC 4 cut(s) 1363, 1442, 2006, 2262
BtsCI GGATG 9 cut(s) 10, 62, 666, 847, 893, 982, 1329, 1919, 2446
BtsI GCAGTG 3 cut(s) 234, 1970, 2841
BtsIMutI CAGTG 8 cut(s) 134, 234, 1970, 2226, 2333, 2823, 2841, 2855
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 8 cut(s) 84, 125, 132, 144, 210, 302, 1209, 1358
CaiI CAGNNNCTG 2 cut(s) 204, 1972
CciI TCATGA 3 cut(s) 702, 930, 1474
CfoI GCGC 3 cut(s) 1010, 1125, 2570
Cfr10I RCCGGY 2 cut(s) 1356, 1391
Cfr13I GGNCC 3 cut(s) 1440, 2005, 2135
CseI GACGC 2 cut(s) 708, 1676
Csp6I GTAC 5 cut(s) 1384, 1564, 1681, 2013, 2370
CspCI CAANNNNNGTGG 2 cut(s) 2883, 2918
CviAII CATG 9 cut(s) 131, 280, 703, 931, 1216, 1475, 1544, 1933, 2962
CviQI GTAC 5 cut(s) 1384, 1564, 1681, 2013, 2370
DraI TTTAAA 2 cut(s) 2028, 2787
DraIII CACNNNGTG 1 cut(s) 2144
EaeI YGGCCR 1 cut(s) 1361
Eam1104I CTCTTC 5 cut(s) 478, 682, 1023, 2444, 2535
EarI CTCTTC 5 cut(s) 478, 682, 1023, 2444, 2535
EciI GGCGGA 1 cut(s) 227
Eco105I TACGTA 1 cut(s) 555
Eco130I CCWWGG 2 cut(s) 419, 2395
Eco147I AGGCCT 1 cut(s) 2262
Eco24I GRGCYC 2 cut(s) 148, 2335
Eco32I GATATC 3 cut(s) 91, 803, 2213
Eco47I GGWCC 1 cut(s) 2135
Eco57I CTGAAG 5 cut(s) 399, 749, 852, 1949, 2202
Eco81I CCTNAGG 1 cut(s) 2912
Eco88I CYCGRG 1 cut(s) 469
EcoO109I RGGNCCY 2 cut(s) 1440, 2005
EcoRI GAATTC 2 cut(s) 1292, 1578
EcoRII CCWGG 5 cut(s) 93, 540, 837, 2262, 2308
EcoRV GATATC 3 cut(s) 91, 803, 2213
EcoT14I CCWWGG 2 cut(s) 419, 2395
EcoT22I ATGCAT 2 cut(s) 562, 1549
EcoT38I GRGCYC 2 cut(s) 148, 2335
ErhI CCWWGG 2 cut(s) 419, 2395
Esp3I CGTCTC 2 cut(s) 494, 1128
FaeI CATG 9 cut(s) 134, 283, 706, 934, 1219, 1478, 1547, 1936, 2965
FalI AAGNNNNNCTT 6 cut(s) 141, 173, 1912, 1944, 2094, 2126
FaqI GGGAC 5 cut(s) 13, 863, 2148, 2592, 2675
FatI CATG 9 cut(s) 130, 279, 702, 930, 1215, 1474, 1543, 1932, 2961
FauI CCCGC 4 cut(s) 135, 917, 2063, 2652
FauNDI CATATG 2 cut(s) 1317, 2622
FbaI TGATCA 1 cut(s) 1884
FblI GTMKAC 1 cut(s) 1690
Fnu4HI GCNGC 9 cut(s) 9, 128, 788, 1098, 1193, 1361, 1530, 2657, 2982
FokI GGATG 9 cut(s) 17, 49, 673, 854, 900, 989, 1336, 1926, 2453
FriOI GRGCYC 2 cut(s) 148, 2335
Fsp4HI GCNGC 9 cut(s) 9, 128, 788, 1098, 1193, 1361, 1530, 2657, 2982
FspBI CTAG 6 cut(s) 362, 453, 1694, 2577, 2720, 2772
GlaI GCGC 3 cut(s) 1009, 1124, 2569
GluI GCNGC 9 cut(s) 9, 128, 788, 1098, 1193, 1361, 1530, 2657, 2982
GsaI CCCAGC 1 cut(s) 2191
GsuI CTGGAG 3 cut(s) 338, 2942, 2951
HaeIII GGCC 4 cut(s) 1363, 1442, 2006, 2262
HapII CCGG 4 cut(s) 32, 1357, 1392, 1638
HgaI GACGC 2 cut(s) 708, 1676
HhaI GCGC 3 cut(s) 1010, 1125, 2570
Hin1I GRCGYC 2 cut(s) 255, 2681
Hin1II CATG 9 cut(s) 134, 283, 706, 934, 1219, 1478, 1547, 1936, 2965
Hin6I GCGC 3 cut(s) 1008, 1123, 2568
HinP1I GCGC 3 cut(s) 1008, 1123, 2568
HincII GTYRAC 1 cut(s) 1990
HindII GTYRAC 1 cut(s) 1990
HindIII AAGCTT 2 cut(s) 1870, 2807
HpaII CCGG 4 cut(s) 32, 1357, 1392, 1638
HphI GGTGA 6 cut(s) 175, 1388, 1442, 2264, 2353, 2486
Hpy166II GTNNAC 4 cut(s) 538, 1558, 1691, 1990
Hpy8I GTNNAC 4 cut(s) 538, 1558, 1691, 1990
HpyAV CCTTC 5 cut(s) 374, 1294, 2015, 2252, 2747
HpyCH4IV ACGT 7 cut(s) 255, 503, 554, 2140, 2233, 2487, 2681
HpyF10VI GCNNNNNNNGC 9 cut(s) 415, 617, 787, 1749, 2075, 2084, 2653, 2710, 2755
HpySE526I ACGT 7 cut(s) 255, 503, 554, 2140, 2233, 2487, 2681
Hsp92I GRCGYC 2 cut(s) 255, 2681
Hsp92II CATG 9 cut(s) 134, 283, 706, 934, 1219, 1478, 1547, 1936, 2965
HspAI GCGC 3 cut(s) 1008, 1123, 2568
KroI GCCGGC 1 cut(s) 1356
KroNI GCCGGC 1 cut(s) 1358
Ksp22I TGATCA 1 cut(s) 1884
LmnI GCTCC 3 cut(s) 1353, 1588, 2653
Lsp1109I GCAGC 6 cut(s) 139, 774, 1109, 1204, 1541, 2643
MaeI CTAG 6 cut(s) 362, 453, 1694, 2577, 2720, 2772
MaeII ACGT 7 cut(s) 255, 503, 554, 2140, 2233, 2487, 2681
MaeIII GTNAC 6 cut(s) 367, 901, 1791, 2221, 2234, 2939
MfeI CAATTG 2 cut(s) 782, 1572
MflI RGATCY 5 cut(s) 214, 244, 383, 921, 2790
MhlI GDGCHC 3 cut(s) 148, 2335, 2804
MlyI GAGTC 8 cut(s) 195, 461, 2090, 2123, 2231, 2413, 2926, 2933
MmeI TCCRAC 5 cut(s) 535, 729, 1429, 1829, 2696
Mph1103I ATGCAT 2 cut(s) 562, 1549
MroNI GCCGGC 1 cut(s) 1356
MroXI GAANNNNTTC 3 cut(s) 762, 2250, 2727
MseI TTAA 9 cut(s) 267, 1310, 1337, 1646, 2027, 2516, 2591, 2696, 2786
MspA1I CMGCKG 2 cut(s) 11, 2087
MspI CCGG 4 cut(s) 32, 1357, 1392, 1638
MspR9I CCNGG 6 cut(s) 32, 95, 542, 839, 2264, 2310
MunI CAATTG 2 cut(s) 782, 1572
Mva1269I GAATGC 5 cut(s) 229, 743, 1259, 2071, 2570
MvaI CCWGG 5 cut(s) 95, 542, 839, 2264, 2310
MvnI CGCG 1 cut(s) 1008
MwoI GCNNNNNNNGC 9 cut(s) 415, 617, 787, 1749, 2075, 2084, 2653, 2710, 2755
NaeI GCCGGC 1 cut(s) 1358
NciI CCSGG 1 cut(s) 32
NdeI CATATG 2 cut(s) 1317, 2622
NgoMIV GCCGGC 1 cut(s) 1356
NlaIII CATG 9 cut(s) 134, 283, 706, 934, 1219, 1478, 1547, 1936, 2965
NlaIV GGNNCC 5 cut(s) 1355, 1441, 2136, 2268, 2836
NmeAIII GCCGAG 1 cut(s) 751
NmuCI GTSAC 5 cut(s) 367, 901, 2221, 2234, 2939
NsiI ATGCAT 2 cut(s) 562, 1549
NspI RCATGY 1 cut(s) 134
PaeI GCATGC 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 469
PagI TCATGA 3 cut(s) 702, 930, 1474
PceI AGGCCT 1 cut(s) 2262
PcsI WCGNNNNNNNCGW 1 cut(s) 560
PctI GAATGC 5 cut(s) 229, 743, 1259, 2071, 2570
PdiI GCCGGC 1 cut(s) 1358
PdmI GAANNNNTTC 3 cut(s) 762, 2250, 2727
PflMI CCANNNNNTGG 3 cut(s) 1349, 2144, 2972
PkrI GCNGC 9 cut(s) 10, 129, 789, 1099, 1194, 1362, 1531, 2658, 2983
PleI GAGTC 8 cut(s) 195, 461, 2089, 2122, 2231, 2413, 2925, 2932
PpsI GAGTC 8 cut(s) 195, 461, 2089, 2122, 2231, 2413, 2925, 2932
Ppu21I YACGTR 2 cut(s) 555, 2141
PshAI GACNNNNGTC 1 cut(s) 506
PshBI ATTAAT 1 cut(s) 2516
Psp6I CCWGG 5 cut(s) 93, 540, 837, 2262, 2308
PspFI CCCAGC 1 cut(s) 2187
PspGI CCWGG 5 cut(s) 93, 540, 837, 2262, 2308
PspN4I GGNNCC 5 cut(s) 1355, 1441, 2136, 2268, 2836
PspPI GGNCC 3 cut(s) 1440, 2005, 2135
PspXI VCTCGAGB 1 cut(s) 469
PstI CTGCAG 3 cut(s) 42, 129, 792
PstNI CAGNNNCTG 2 cut(s) 204, 1972
PsuI RGATCY 5 cut(s) 214, 244, 383, 921, 2790
PvuII CAGCTG 1 cut(s) 2087
RsaI GTAC 5 cut(s) 1385, 1565, 1682, 2014, 2371
RsaNI GTAC 5 cut(s) 1384, 1564, 1681, 2013, 2370
SaqAI TTAA 9 cut(s) 267, 1310, 1337, 1646, 2027, 2516, 2591, 2696, 2786
SatI GCNGC 9 cut(s) 9, 128, 788, 1098, 1193, 1361, 1530, 2657, 2982
Sau96I GGNCC 3 cut(s) 1440, 2005, 2135
ScaI AGTACT 1 cut(s) 1385
SchI GAGTC 8 cut(s) 195, 461, 2090, 2123, 2231, 2413, 2926, 2933
ScrFI CCNGG 6 cut(s) 32, 95, 542, 839, 2264, 2310
SduI GDGCHC 3 cut(s) 148, 2335, 2804
SfcI CTRYAG 5 cut(s) 12, 38, 125, 372, 788
Sfr274I CTCGAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 2135
SlaI CTCGAG 1 cut(s) 469
SmlI CTYRAG 8 cut(s) 147, 218, 469, 1753, 1940, 2111, 2414, 2642
SmoI CTYRAG 8 cut(s) 147, 218, 469, 1753, 1940, 2111, 2414, 2642
SnaBI TACGTA 1 cut(s) 555
SphI GCATGC 1 cut(s) 134
SseBI AGGCCT 1 cut(s) 2262
SsiI CCGC 7 cut(s) 9, 142, 212, 910, 1360, 2056, 2659
SspI AATATT 1 cut(s) 2050
SspMI CTAG 6 cut(s) 362, 453, 1694, 2577, 2720, 2772
StuI AGGCCT 1 cut(s) 2262
StyD4I CCNGG 6 cut(s) 30, 93, 540, 837, 2262, 2308
StyI CCWWGG 2 cut(s) 419, 2395
TaiI ACGT 7 cut(s) 258, 506, 557, 2143, 2236, 2490, 2684
TaqI TCGA 6 cut(s) 51, 430, 465, 470, 651, 2505
TatI WGTACW 3 cut(s) 1383, 1563, 2369
TauI GCSGC 2 cut(s) 11, 1363
Tru1I TTAA 9 cut(s) 267, 1310, 1337, 1646, 2027, 2516, 2591, 2696, 2786
Tru9I TTAA 9 cut(s) 267, 1310, 1337, 1646, 2027, 2516, 2591, 2696, 2786
TscAI CASTG 8 cut(s) 141, 234, 1977, 2226, 2333, 2823, 2848, 2862
TseFI GTSAC 5 cut(s) 367, 901, 2221, 2234, 2939
TseI GCWGC 7 cut(s) 127, 787, 1097, 1192, 1529, 2656, 2981
Tsp45I GTSAC 5 cut(s) 367, 901, 2221, 2234, 2939
TspGWI ACGGA 2 cut(s) 989, 1972
TspRI CASTG 8 cut(s) 141, 234, 1977, 2226, 2333, 2823, 2848, 2862
Van91I CCANNNNNTGG 3 cut(s) 1349, 2144, 2972
VpaK11BI GGWCC 1 cut(s) 2135
VspI ATTAAT 1 cut(s) 2516
XapI RAATTY 6 cut(s) 946, 1292, 1578, 2198, 2250, 2727
XbaI TCTAGA 1 cut(s) 2719
XceI RCATGY 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 1690
XmnI GAANNNNTTC 3 cut(s) 762, 2250, 2727
XspI CTAG 6 cut(s) 362, 453, 1694, 2577, 2720, 2772
ZraI GACGTC 2 cut(s) 256, 2682
ZrmI AGTACT 1 cut(s) 1385
Zsp2I ATGCAT 2 cut(s) 562, 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.